Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,169,658 C→A 6.7% intergenic (+47/+35) yegS → / ← gatR phosphatidylglycerol kinase, metal‑dependent/pseudogene, repressor for gat operon; interrupted by IS3; split galactitol utilization operon repressor, fragment 2; split galactitol utilization operon repressor, interrupted

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,169,6580CA 6.7% 302.0 / 5.6 105intergenic (+47/+35)yegS/gatRphosphatidylglycerol kinase, metal‑dependent/pseudogene, repressor for gat operon; interrupted by IS3; split galactitol utilization operon repressor, fragment 2; split galactitol utilization operon repressor, interrupted
Reads supporting (aligned to +/- strand):  ref base C (43/53);  new base A (5/3);  total (49/56)
Fisher's exact test for biased strand distribution p-value = 4.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 106 total aligned reads displayed.

ATCTTGATGGCGAACCGTTGAGTGGGCAAAATTTTCATATTGAAATACTTCCGGCAGCGTTGCGTTGTCGATTACCACCAGATTGTCCACTGTTGCGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCATCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGATTAAAA  >  NC_000913/2169511‑2169807
                                                                                                                                                   |                                                                                                                                                     
aTCTTGATGGCGAACCGTTGAGTGGGCAAAATTATCATATTGAAATACTTCCGGCAGCGTTGCGTTGTCGATTACCACCAGATTGTCCACTGTTGCGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCgg                                                                                                                                                     <  2:422086/150‑1 (MQ=255)
  cTTGATGGCGAACCGTTGAGTGGGCAAAATTTTCATATTGAAATACTTCCGGCAGCGTTGCGTTGTCGATTACCACCAGATTGTCCACTGTTGCGTTAATCAAACTATTCTCTCTTGAACCAGGGAGCATCCGTAATGCCGGATGCGgtg                                                                                                                                                   >  1:625955/1‑150 (MQ=255)
  attGATGGCGAACCGTTGAGTGGGCAAAATTTTCATATTGAAATACTTCCGGCAGCGTTGCGTTGTCGATTACCACCAGATTGTCCACTGTTGCGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGTATGCGgtg                                                                                                                                                   <  1:1690162/149‑1 (MQ=255)
    tGATGGCGAACCGTTGAGTGGGCAAAATTTTCATATTGAAATACTTCCGGCAGCGTTGCGTTGTCGATTACCACCAGATTGTCCACTGTTGCGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTa                                                                                                                                                 <  2:1266877/150‑1 (MQ=255)
      aTGGCGAACCGTTGAGTGGGCAAAATTTTCATATTGAAATACTTCCGGCAGCGTTGCGTTGTCGATTACCACCAGATTGTCCACTGTTGCGTTAATCAAACTATTCTCTTTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTaaa                                                                                                                                               >  2:1585882/1‑150 (MQ=255)
        ggCGATCTGTTGAGGGGGCAAAATTTTCATATTGAAATACTTCCGGCAGCGTTGCGTTGTAGATTACCACCAGATTGTCCACTGTTGCGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAaca                                                                                                                                             <  2:238435/150‑1 (MQ=255)
          cGAACCGTTGAGTGGGCAAAATTTTCATATTGAAATACTTCCGGCAGCGTTGCGTTGTCGATTACCACCAGATTGTCCACTGTTGCGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACAcc                                                                                                                                           <  1:1716577/150‑1 (MQ=255)
               cGTTGAGTGGGCAAAATTTTCATATTGAAATACTTCCGGCAGCGTTGCGTTGTCGATTACCACCAGATTGTCCACTGTTGCGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATc                                                                                                                                      >  1:125548/1‑150 (MQ=255)
               cGTTGAGTGGGCAAAATTTTCATATTGAAATACTTCCGGCAGCGTTGCGTTGTCGATTACCACCAGATTGTCCACTGTTGCGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCAGTGTAAACACCTTATc                                                                                                                                      <  2:1105772/150‑1 (MQ=255)
                   tattGGTCAAAATTTTCATATTGAAATACTTCCTGCAGCGTTGCGTTGTCGATTACCACCAGATTGTCCACTGTTGCGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATTATTTGTAAACACCTTATCCGGc                                                                                                                                  <  1:1444662/147‑1 (MQ=255)
                    aGTGGGCAAAATTTTCATATTGAAATACTTCCGGCAGCGTTGCGTTGTCGATTACCACCAGATTGTCCACTGTTGCGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCa                                                                                                                                 <  2:585854/150‑1 (MQ=255)
                     gTGGGCAAAATTTTCATATTGAAATACTTCCGGCAGCGTTGCGTTGTCGATTACCACCAGATTGTCCACTGTTGCGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTACACACCTTCTCCTGCAt                                                                                                                                >  1:266367/1‑150 (MQ=255)
                     gTGGGCAAAATTTTCATATTGAAATACTTCCGGCAGCGTTGCGGTGTCGATTACCACCAGATTGTCCACTGTTGCGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATAAGGTGTTTACACCGCATCCGGCAt                                                                                                                                <  2:704355/150‑1 (MQ=255)
                      tGGGCAAAATTTTCATATTGAAATACTTCCGGCAGCGTTGCGTTGTCGAATACCACCAGATTGTCCACTGTTGCGTTAATCAAACTATGCTCTCATGAACCAGGGAGAATCCGTAATGCCGGATAAGGTGTTTACACCGCATCCGGCAtt                                                                                                                               <  1:1101502/150‑2 (MQ=255)
                       gggCAAAATTTTCATATTGAAATACTTCCGGCAGCGTTGCGTTGTCGATTACCACCAGATTGTCCACTGTTGCGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATAc                                                                                                                              <  2:1079408/150‑1 (MQ=255)
                        ggCAAAATTTTCATATTGAAATACTTCCGGCAGCGTTGCGTTGTCGATTACCACCAGATTGTCCACTGTTGCGTTAATCAAACTATTCTCTCTTGAACCAGGGAGATTCAGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACa                                                                                                                             >  2:327830/1‑150 (MQ=255)
                            aaaTTTTCATATTGAAATACTTCCGGCAGCGTTGCGTTGTCGATTACCACCAGATTGTCCACTGTTGCGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAAc                                                                                                                         <  1:216800/150‑1 (MQ=255)
                              aTTTTCATATTGAAATACTTCCGGCAGCGTTGCGTTGTCGATTACCACCAGATTGTCCACTGTTGCGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACaa                                                                                                                       >  1:390626/1‑150 (MQ=255)
                               ttttCATATTGAAATACTTCCGGCAGCGTTGCGTTGTCGATTACCACCAGATTGTCCACTGTTGCGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAAt                                                                                                                      <  1:1297793/150‑1 (MQ=255)
                                     tatTGAAATACTTCCGGCAGCGTTGCGTTGTCGATTACCACCAGATTGTCCACTGTTGCGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAACGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTaca                                                                                                                >  2:585248/1‑150 (MQ=255)
                                            aTACTTCCGGCAGCGTTGCGTTGTCGATTACCACCAGATTGTCCACTGTTGCGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGtaat                                                                                                         <  1:1105075/150‑1 (MQ=255)
                                               cTTCCGGCAGCGTTGCGTTGTCGATTACCACCAGATTGTCCACTGTTGCGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGtaattaa                                                                                                      >  1:918776/1‑150 (MQ=255)
                                               cTTCCGGCAGCGTTGCGTTGTCGATTACCACCAGATTGTCCACTGTTGCGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGtaattaa                                                                                                      >  2:1530677/1‑150 (MQ=255)
                                                   cGGCAGCGTTGCGTTGTCGATTACCACCAGATTGTCCACTGTTGCGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGTAATTAAtttt                                                                                                  <  2:1549800/150‑1 (MQ=255)
                                                    ggCAGCGTTGCGTTGTCGATTACCACCAGATTGTCCACTGTTGCGTTAATCAAACTATTCTCTCTTGAACCAGTGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTa                                                                                                 <  1:27331/150‑1 (MQ=255)
                                                      cAGCGTTGCGTTGTCGATTACCACCAGATTGTCCACTGTTGCGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTAcc                                                                                               >  1:1144899/1‑150 (MQ=255)
                                                       aGCGTTGCGTTGTCGATTACCACCAGATTGTCCACTGTTGCGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTAccc                                                                                              <  2:918776/150‑1 (MQ=255)
                                                        gcgttgcgttGTCGATTACCACCAGATTGTCCACTGTTGCGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCcg                                                                                             <  2:1437295/150‑1 (MQ=255)
                                                        gcgttgcgttGTCGATTACCACCAGATTGTCCACTGTTGCGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCcg                                                                                             <  2:1445072/150‑1 (MQ=255)
                                                        gcgttgcgttGTCGATTACCACCAGATTGTCCACTGTTGCGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCcg                                                                                             <  2:640023/150‑1 (MQ=255)
                                                            tgcgttgTCGATTACCACCAGATTGTCCACTGTTGCGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGtc                                                                                         <  1:1675274/150‑1 (MQ=255)
                                                             gcgttgTCGATTACCACCAGATTGTCCACTGTTGCGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGtct                                                                                        <  2:1473523/150‑1 (MQ=255)
                                                             gcgttgTCGATTACCACCAGATTGTCCACTGTTGCGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGtct                                                                                        <  1:1578817/150‑1 (MQ=255)
                                                               gttgTCGATTACCACCAGATTGTCCACTGTTGCGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGtcttc                                                                                      >  1:754361/1‑150 (MQ=255)
                                                                ttgTCGATTACCACCAGATTGTCCACTGTTGCGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGtcttct                                                                                     >  2:221250/1‑150 (MQ=255)
                                                                ttgTAGATTACCACCAGATTGTCCACTGTTGCGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGtcttct                                                                                     <  2:1318022/150‑1 (MQ=255)
                                                                 tgTCGATTACCACCAGATTGTCCACTGTTGCGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTg                                                                                    <  1:795052/150‑1 (MQ=255)
                                                                  gTCGATTACCACCAGATTGTCCACTGTTGCGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGc                                                                                   <  1:799193/150‑1 (MQ=255)
                                                                  gTCGATTACCACCAGATTGTCCACTGTTGCGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGc                                                                                   <  2:937976/150‑1 (MQ=255)
                                                                  gTCGATTACCACCAGATTGTCCACTGTTGCGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACATTATCCGGCATACAGAACAATACTACACAGTAATTAATTTTACCCGCGTCTTCTGc                                                                                   >  2:439998/1‑150 (MQ=255)
                                                                      aTTACCACCAGATTGTCCACTGTTGCGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATg                                                                               <  1:469530/150‑1 (MQ=255)
                                                                         accaccAGATTGTCCACTGTTGCGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCa                                                                            <  2:420962/150‑1 (MQ=255)
                                                                         accaccAGATTGTCCACTGTTGCGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCa                                                                            >  1:1166857/1‑150 (MQ=255)
                                                                          ccaccaGATTGTCCACTGTTGCGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGATCAATACTAGACCGTAATTAATTTTACCCGCGTCTTCTGCAATGACAg                                                                           >  2:1043088/1‑150 (MQ=255)
                                                                          ccaccaGATTGTCCACTGTTGCGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAg                                                                           >  2:45435/1‑150 (MQ=255)
                                                                          ccaccaGATTGTCCACTGTTGCGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAg                                                                           >  2:782588/1‑150 (MQ=255)
                                                                          ccaccaGATTGTCCACTGTTGCGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAg                                                                           >  2:1242518/1‑150 (MQ=255)
                                                                          ccaccaGATTGTCCACTGTTGCGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAg                                                                           >  2:1483605/1‑150 (MQ=255)
                                                                           caccaGATTGTCCACTGTTGCGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGt                                                                          >  2:1445199/1‑150 (MQ=255)
                                                                            accaGATTGTCCACTGTTGCGTTAATCAAACTATTCTCTTTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGtt                                                                         <  1:1585882/150‑1 (MQ=255)
                                                                            accaGATTGTCCACTGTTGCGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGTATGCGGTGTAAACACCTTATACGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGtt                                                                         <  1:1048625/150‑1 (MQ=255)
                                                                            accaGATTGTCCACTGTTGCGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGtt                                                                         <  1:1456789/150‑1 (MQ=255)
                                                                             ccaGATTGTCCACTGTTGCGTTATTCAAACTATTCTCACTTGAACCAGGCAGAATCCGTAATGCCGGATGCGTTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGAAAATAATTTTACCCGCGACTTCTCCAATGCCAGttt                                                                        >  1:280794/1‑150 (MQ=255)
                                                                                 aTTGTCCACTGTTGCGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATct                                                                    <  1:439998/150‑2 (MQ=255)
                                                                                    gTCCACTGTTGCTTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCAcc                                                                 <  2:1166130/150‑1 (MQ=255)
                                                                                      ccACTGTTGCGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACctc                                                               <  2:154091/150‑1 (MQ=255)
                                                                                         cTGTTGCGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGa                                                            <  1:1145886/150‑1 (MQ=255)
                                                                                         cTGTTGCGTTAATCAAACTATTATCTCTTGAAAAAGGGAGAATCAGTAATTACGGATGCGGTGTAAACACCTTATCCGGCATACAGAAAAATACTAAACCGTAATTAATTTTACCAGAGTCTTATGCAATGACAGTATATCAACCTCTGa                                                            >  1:568690/1‑150 (MQ=255)
                                                                                          tGTTGCGTTAATCAAACTATTCTCTCTTGAACCAGGGTGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGac                                                           >  2:1547339/1‑150 (MQ=255)
                                                                                            ttGCGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCACCTCTGAcaca                                                        <  2:1247742/150‑1 (MQ=255)
                                                                                                gTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGca                                                     <  2:1546956/150‑1 (MQ=255)
                                                                                                     tCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGTCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCatcatc                                                <  1:1118239/150‑1 (MQ=255)
                                                                                                      cAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCATCATCg                                               >  2:956599/1‑150 (MQ=255)
                                                                                                      cAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCATCATCg                                               <  1:1029781/150‑1 (MQ=255)
                                                                                                      cAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCATCATCg                                               >  1:615447/1‑150 (MQ=255)
                                                                                                       aaaCTATTCTCTCTTTAACCAGGAAGAAACCGTAATGCCGGATGAGCTGTAAACACCTTATCCGGCATACAGAACAATACTACACCCTAATTATGTTAACACGCGTCTTCTGCAATGCAAGTTTATCCACCTCTGACAAAGCATCATCgg                                              >  1:115658/1‑150 (MQ=255)
                                                                                                        aaCTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCATCATCGGt                                             <  2:1144899/150‑1 (MQ=255)
                                                                                                          cTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCATCATCGGtaa                                           <  2:1375647/150‑1 (MQ=255)
                                                                                                           tATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTGTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCATCATCGGtaat                                          <  2:629724/150‑1 (MQ=255)
                                                                                                           tATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCATCATCGGtaat                                          <  1:1530677/150‑1 (MQ=255)
                                                                                                            aTTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCATCATCGGtaata                                         <  2:286384/150‑1 (MQ=255)
                                                                                                            aTTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCATCATCGGtaata                                         <  1:1631412/150‑1 (MQ=255)
                                                                                                                 ctctTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCATCATCGGTaataataat                                    >  2:1688739/1‑150 (MQ=255)
                                                                                                                 ctctTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCATCATCGGTaataataat                                    >  1:764894/1‑150 (MQ=255)
                                                                                                                  tctTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCATCATCGGTAATAATAATg                                   >  2:986203/1‑150 (MQ=255)
                                                                                                                   ctTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCATCATCGGTAATAATAATGt                                  >  1:1682766/1‑150 (MQ=255)
                                                                                                                      gAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCATCATCGGTAATAATAATGTCaa                               <  1:1460363/150‑1 (MQ=255)
                                                                                                                         ccAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCATCATCGGTAATAATAATGTCAAttt                            >  1:1688247/1‑150 (MQ=255)
                                                                                                                         ccAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCAACCTCTGACACAGCATCATCGGTAATAATAATGTCAAttt                            >  2:872708/1‑150 (MQ=255)
                                                                                                                          cAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCATCATCGGTAATAATAATGTCAAtttt                           >  2:871712/1‑150 (MQ=255)
                                                                                                                           aGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCATCATCGGTAATAATAATGTCAAttttt                          >  1:1017556/1‑150 (MQ=255)
                                                                                                                           aGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCATCATCGGTAATAATAATGTCAAttttt                          >  2:898787/1‑150 (MQ=255)
                                                                                                                           aGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCATCATCGGTAATAATAATGTCAAttttt                          >  2:572531/1‑150 (MQ=255)
                                                                                                                            gggAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCTGCATACAGTACAATTCTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCATCATCGGTAATAATAATGTCAAtttttt                         <  2:929759/150‑1 (MQ=255)
                                                                                                                             ggAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCATCATCGGTAATAATAATGTCAATTTTTTc                        <  1:1242518/150‑1 (MQ=255)
                                                                                                                               agaATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCATCATCGGTAATAATAATGTCAATTTTTTCGa                      >  2:1506983/1‑150 (MQ=255)
                                                                                                                                 aaTCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATAATACACCGTAATTAATTTTACCCGCGTATTCTGCAATTCCAGTTTATCCACCTCTGACACAGCATCATCGGTAATAATAATGTCAATTTTTTCGAtt                    <  1:1578076/150‑1 (MQ=255)
                                                                                                                                    ccGTAATGCCGGATGCGGTGTAATCCCCTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATCCCAGTTTATCCACCTCTCACAAAGCATCATCTGTAATAATAATGTCAATTATTTCGATTGgt                 >  2:727411/1‑150 (MQ=255)
                                                                                                                                     cGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACCGCATCATCGGTAATAATAATGTCAATTTTTTCGATTGgta                >  2:465515/1‑150 (MQ=255)
                                                                                                                                     agTAATGCCTGATGCTTTTTAAACACCTTATACTTCATACAGAACAATACTACACCGTAATTAATTTTACCCTCGTCTTCTGCAATGCCATTTTATCCAACTCTGACACATCATCATCTGTAATAATAATTTCAATTTTTTCGATTGgta                <  2:568690/149‑1 (MQ=255)
                                                                                                                                      gTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCATCATCGGTAATAATAATGTCAATTTTTTCGATTGgtag               <  1:956599/150‑1 (MQ=255)
                                                                                                                                        aaTGCCGGATGTGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCATCATCGGTAATAATAATGTCAATTTTTTCGATTGgtagta             >  2:705677/1‑150 (MQ=255)
                                                                                                                                         aTGCCGGATAAGGTGTTTACACCGCATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCCCCTCTGACACAGCATCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTAc            >  2:789330/1‑150 (MQ=255)
                                                                                                                                         aTGCCGGATAAGGTGTTTACACCGCATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCATCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTAc            >  2:1462317/1‑150 (MQ=255)
                                                                                                                                         aTGCCGGATAAGGTGTTTACACCGCATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCATCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTAc            >  2:331456/1‑150 (MQ=255)
                                                                                                                                          tGCCGGATAAGGTGTTTACACCGCATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCATCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTAcc           >  1:206594/1‑150 (MQ=255)
                                                                                                                                              ggATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCATCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGAt       >  2:1586978/1‑150 (MQ=255)
                                                                                                                                              ctaagCGGTGTAAATAGATTATCCGGGATAAAGATCAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATTCCAGTTTATCCACCTCTGAGAATGCATCATCGGTAATAATATTGTCAATTTTTTCGATTGGTAGTACCTGAt       <  2:115658/146‑1 (MQ=255)
                                                                                                                                               gATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCATCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGAtt      >  2:768009/1‑150 (MQ=255)
                                                                                                                                                   cGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCATCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGATTaaaa  >  1:566211/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
ATCTTGATGGCGAACCGTTGAGTGGGCAAAATTTTCATATTGAAATACTTCCGGCAGCGTTGCGTTGTCGATTACCACCAGATTGTCCACTGTTGCGTTAATCAAACTATTCTCTCTTGAACCAGGGAGAATCCGTAATGCCGGATGCGGTGTAAACACCTTATCCGGCATACAGAACAATACTACACCGTAATTAATTTTACCCGCGTCTTCTGCAATGCCAGTTTATCCACCTCTGACACAGCATCATCGGTAATAATAATGTCAATTTTTTCGATTGGTAGTACCTGATTAAAA  >  NC_000913/2169511‑2169807

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: