Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 202,043 C→A 5.8% intergenic (+47/‑58) lpxD → / → fabZ UDP‑3‑O‑(3‑hydroxymyristoyl)‑glucosamine N‑acyltransferase/(3R)‑hydroxymyristol acyl carrier protein dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913202,0430CA 5.8% 329.1 / 4.6 107intergenic (+47/‑58)lpxD/fabZUDP‑3‑O‑(3‑hydroxymyristoyl)‑glucosamine N‑acyltransferase/(3R)‑hydroxymyristol acyl carrier protein dehydratase
Reads supporting (aligned to +/- strand):  ref base C (50/50);  new base A (4/3);  total (54/53)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 108 total aligned reads displayed.

ACCCAACAAAGTCTGGCGCAAAACCGCTGCACTGGTGATGAACATTGATGACATGAGCAAGCGTCTGAAATCGCTTGAGCGCAAGGTTAATCAACAAGACTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAAGAGTATCTTGACTACTAACACTCATACTCTGCAGATTGAAGAGATTTTAGAACTTCTGCCGCACCGTTTCCCGTTCTTACTGGTGGATCGCGT  >  NC_000913/201894‑202186
                                                                                                                                                     |                                                                                                                                               
aCCCAACAAAGTCTGGCGCAAAACCGCTGCACTGGTGATGAACATTGATGACATGAGCAAGCGTCTGAAATCGCTTGAGCGCAAGGTTAATCAACAAGACTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTAc                                                                                                                                                 <  2:501801/150‑1 (MQ=255)
 cccAACAAAGTCTGGCGCAAAACCGCTGCACTGGTGATGAACATTGATGACATGAGCAAGCGTCTGAAATCGCTTGAGCGCAAGGTTAATCAACAAGACTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACg                                                                                                                                                >  1:1259470/1‑150 (MQ=255)
  ccAACAAAGTCTGGCGCAAAACCGCTGCACTGGTGATGAACATTGATGACATGAGCAAGCGTCTGAAATCACTTGAGCGCAAGGTTAATCAACAAGACTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGa                                                                                                                                               >  1:1418525/1‑150 (MQ=255)
   caacaaAGTCTGGCGCAAAACCGCTGCACTGGTGATGAACATTGATGACATGAGCAAGCGACTGAAAACGCTTGAGCGCAAGGTTAATCAACATGACTAACGTTACATCTTTTGTTCCACAAACTTTACGGCCTGTCTCATTCTTACGAt                                                                                                                                              >  2:228141/1‑150 (MQ=255)
          gTCTGGCGCAAAACCGCTGCACTGGTGATGAACATTGATGACATGAGCAAGCGTCTGAAATCGCTTGAGCGCAAGGTTAATCAACAAGACTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCa                                                                                                                                       >  2:775391/1‑150 (MQ=255)
            cTGGCGCAAAACCGCTGCACTGGTGATGAACATTGATGACATGAGCAAGCGTCTGAAATCGCTTGAGCGCAAGGTTAATCAACAAGACTAACGTTGCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAgg                                                                                                                                     >  1:262104/1‑150 (MQ=255)
              ggCGCAAAACCGCTGCACTGGTGATGAACATTGATGACATGAGCAAGCGTCTGAAATCGCTTGAGCGCAAGGTTAATCAACAAGACTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGcc                                                                                                                                   >  2:1192585/1‑150 (MQ=255)
              ggCGCAAAACCGCTGCACTGGTGATGAACATTGATGACATGAGCAAGCGTCTGAAATCGCTTGAGCGCAAGGTTAATCAACAAGACTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGcc                                                                                                                                   >  2:272746/1‑150 (MQ=255)
               gcgcAAAACCGCTGCACTGGTGATGAACATTGATGACATGAGCAAGCGTCTGAAATCGCTTGAGCGCAAGGTTAATCAACAAGACTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGCCGCAATCGTAAGAATGAGACAGGCCg                                                                                                                                  <  2:566764/150‑1 (MQ=255)
                  cAAAACCGCTGCACTGGTGATGAACATTGATGACATGAGCAAGCGTCTGAAATCGCTTGAGCGCAAGGTTAATCAACAAGACTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGCCGCAATCGTAAGAATGAGACAGGCCgtaa                                                                                                                               <  2:690344/150‑3 (MQ=38)
                   ataaacGCTGCACTGGTGATTAACATTGATGACATGAGCAAGCGTCTGAAATCGCTTGAGCGCAAGGTTAATCAACAAGACTAACGTTCCATCTTTTGTTCGCCTAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGtt                                                                                                                              <  1:137813/145‑1 (MQ=255)
                    aaaCCGCTGCACTGGTGATGAACATTGATGACATGAGCAAGCGTCTGAAATCGCTTGAGCGCAAGGTTAATCAACAAGACTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGtta                                                                                                                             <  2:1135410/150‑1 (MQ=255)
                     aaCCGCTGCACTGGTGATGAACATTGATGACATGAGCAAGCGTCTGAAATCGCTTGAGCGCAAGGTTAATCAACAAGACTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGttat                                                                                                                            >  2:67672/1‑150 (MQ=255)
                      aCCGCTGCACTGGTGATGAACATTGATGACATGAGCAAGCGTCTGAAATCGCTTGAGCGCAAGGTTAATCAACAAGACTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGttatt                                                                                                                           >  2:1129374/1‑150 (MQ=255)
                      aCCGCTGCACTGGTGATGAACATTGATGACATGAGCAAGCGTCTGAAATCGCTTGAGCGCAAGGTTAATCAACAAGACTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGttatt                                                                                                                           >  2:1123566/1‑150 (MQ=255)
                        cGCTGCACTGGTGATGAACATTGATGACATGAGCAAGCGTCTGAAATCGCTTGAGTGCAAGGTTAATCAACAAGACTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTtattat                                                                                                                         <  2:703776/150‑1 (MQ=255)
                          cTGCACTGGTGATGATCATTGATGTCATTAGCAAGCGTCTGAAATCGCTTGAGCGCAAGGGTAATCAACAAGACTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTg                                                                                                                       <  2:1591327/150‑1 (MQ=255)
                           tGCACTGGTGATGAACATTGATGACATGAGCAAGCGTCTGAAATCGCTTGAGCGCAAGGTTAATCAACAAGACTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGt                                                                                                                      <  2:1063876/150‑1 (MQ=255)
                           tGCACTGGTGAGGAACATTGATGACATGAGCAAGCGTCTGAAATCGCTTGAGCGCAAGGTTAATCAACAAGACTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGt                                                                                                                      <  2:1058307/150‑1 (MQ=255)
                            tgaCTGGTGATGAACATTGATGTCATGATCAATCGTCTGAAATGGCTTGAGCGCAATGTTAATCAACAAGTCTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTAAGATTGCGGCAGGCCGTGTTATTATTGTc                                                                                                                     <  2:1425682/148‑1 (MQ=255)
                            gCACTGGTGATGAACATTGATGACATGAGCAAGCGTCTGAAATCGCTTGAGCGCAAGGTTAATCAACAAGACTAACGTTCCATCTTTTGTTCGCCAAACTATACGGCCTGTCTCATTCTTAAGATTGCGGCAGGCCGTGTTATTATTGTc                                                                                                                     >  2:955813/1‑150 (MQ=255)
                             cACTGGTGATGAACATTGATGACATGAGCAAGCGTCTGAAATCGCTTGAGCGCAAGGTTAATCAACAAGACTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCg                                                                                                                    <  1:594272/150‑1 (MQ=255)
                             cACTGGTGATGAACATTGATGACATGAGCAAGCGTCTGAAATCGCTTGAGCGCAAGGTTAATCAACAAGACTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCg                                                                                                                    >  1:1072095/1‑150 (MQ=255)
                             cACTGGTGATGAACATTGATGACATGAGCAAGCGTCTGAAATCGCTTGAGCGCAAGGTTAATCAACAAGACTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCg                                                                                                                    <  1:1185602/150‑1 (MQ=255)
                             cACTGGTGATGAACATTGATGACATGAGCAAGCGTCTGAAATCGCTTGAGCGCAAGGTTAATCAACAAGACTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCg                                                                                                                    <  1:357426/150‑1 (MQ=255)
                              aCTGGTGATGAACATTGATGACATGAGCAAGCGTCTGAAATCGCTTGAGCGCAAGGTTAATCAACAAGACTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGt                                                                                                                   <  1:961121/150‑1 (MQ=255)
                               cTGGTGATGAACATTGATGACATGAGCAAGCGTCTGAAATCGCTTGAGCGCAAGGTTAATCAACAAGACTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATACTTACGATTGCGGCAGGCCGTGTTATTAATGTCGtt                                                                                                                  >  2:387370/1‑150 (MQ=255)
                                 ggTGATGAACATTGATGACATGCGCACGCGTCTGAAATCGCTTGAGCGCAAGGTTAATCCACAAGACTAACGTTCCATCTTTTGTTCGCCAAACTGTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTc                                                                                                                <  2:224174/150‑1 (MQ=255)
                                 ggTGATGAACATTGATGACATGAGCAAGCGTCTGAAATCGCTTGAGCGCAAGGTTAATCAACAAGACTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTc                                                                                                                <  2:809155/150‑1 (MQ=255)
                                   tgatgaACATTGATGACATGAGCAAGCGTCTGAAATCGCTTGAGCGCAAGGTTAATCAACAAGACTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCtt                                                                                                              <  1:189291/150‑1 (MQ=255)
                                    gatgaACATTGATGACATGAGCAAGCGTCTGAAATCGCTTGAGCGCAAGGTTAATCAACAAGACTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTta                                                                                                             >  1:1388678/1‑150 (MQ=255)
                                        aaCATTGATGACATGAGCAAGCGTCTGAAATCGCTTGAGCGCAAGGTTAATCAACAAGACTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGttatta                                                                                                                          <  1:1152044/133‑1 (MQ=255)
                                        aaCATTGATGACATGAGCAAGCGTCTGAAATCGCTTGAGCGCAAGGTTAATCAACAAGACTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGttatta                                                                                                                          >  2:1152044/1‑133 (MQ=255)
                                        aaCATTGATGACATGAGCAAGCGTCTGAAATCGCTTGAGCGCAAGGTTAATCAACAAGACTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATAtt                                                                                                         <  2:1259470/150‑1 (MQ=255)
                                         aCATTGATGACATGAGCAAGCGTCTGAAATCGCTTGAGCGCAAGGTTAATCAACAAGACTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATAttt                                                                                                        >  2:1527424/1‑150 (MQ=255)
                                          aatttctgaCATTAGCAAGCGGATGAAATCGCTTTAGCTCAATGGTACTCAACAAGAATAACGTTCCATCTTTTGTTCGCCAAAATTTACGTCCTGTATCATTCTTACGATTGCGGCAGGCCGTTTTATTATTTTCGTTTCTTATAtttt                                                                                                       <  1:126272/144‑1 (MQ=255)
                                           aTTGATGACATGAGCAAGCGTCTGAAATCGCTTGAGCGCAAGGTTAATCAACAAGACTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTg                                                                                                      <  1:1529157/150‑1 (MQ=255)
                                           aTTGATGACATGAGCAAGCGTCTGAAATCGCTTGAGCGCAAGGTTAATCAACAAGACTAACGTTCCATCTGTTGTACGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTAATAGTGTCGTTTCTTATATTTTg                                                                                                      >  2:843660/1‑150 (MQ=255)
                                            ttGATGACATGAGCAAGCGTCTGAAATCGCTTGAGCGCAAGGTTAATCAACAAGACTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGa                                                                                                     <  1:549344/150‑1 (MQ=255)
                                            ttGATGACATGAGCAAGCGTCTGAAATCGCTTGAGCGCAAGGTTAATCAACAAGACTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGa                                                                                                     <  2:119156/150‑1 (MQ=255)
                                                tgaCATGAGCAAGCGTCTGAAATCGCTTGAGCGCAAGGTTAATCAACAAGACTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAgg                                                                                                 >  1:1035003/1‑150 (MQ=255)
                                                tgaCATGAGCAAGCGTCTGAAATCGCTTGAGCGCAAGGTTAATCAACAAGACTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAgg                                                                                                 <  1:319171/150‑1 (MQ=255)
                                                tgaCATGAGCAAGCGTCTGAAATCGCTTGAGCGCAAGGTTAATCAACAAGACTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAgg                                                                                                 <  1:1572417/150‑1 (MQ=255)
                                                 gaCATGAGCAAGCGTCTGAAATCGCTTGAGCGCAAGGTTAATCAACAAGACTAACGTTCCATCTTTTGTTCGCCAAACTTAACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTAATTATTGTCGTTTCTTATATTTTGACAGGa                                                                                                >  1:260736/1‑150 (MQ=255)
                                                   cATGAGCAAGCGTCTGAAATCGCTTGAGCGCAAGGTTAATCAACAAGACTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAag                                                                                              <  2:542692/150‑1 (MQ=255)
                                                     tGAGCAAGCGTCTGAAATCGCTTGAGCGCAAGGTTAATCAACAAGACTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAagag                                                                                            >  1:1635201/1‑150 (MQ=255)
                                                         cAAGCGTCTGAAATCGCTTGAGCGCAAGGTTAATCAACAAGACTAACGTTCCAACTTTTGTTCGCCAAACTTTACGGCCTGTATCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAAGAGTAta                                                                                        >  2:1712640/1‑149 (MQ=255)
                                                          aaGCTTCTGAAATCGCTTGATCGCAAGGTTAATCAACAATACTATCGTTCCATCTTTTTTTCTCCAAACTTTACGTCCTGTCTGATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAAGAGTATCt                                                                                       <  1:287091/150‑1 (MQ=255)
                                                          aaGCGTCTGAAATCGCTTGAGCGCAAGGTTAATCAACAAGACTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAAGAGTATCt                                                                                       <  2:530852/150‑1 (MQ=255)
                                                           aGCGTCTGAAATCGCTTGAGCGCAAGGTTAATCAACAAGACTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAAGAGTATCtt                                                                                      >  1:925554/1‑150 (MQ=255)
                                                            gCGTCTGAAATCTCTTGAGCTCAAGGTTAATCAACAATACTAACGTTCTATCTTTTGTTCGCCAAACTTTACGGCCTTTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAAGTGTATCTTg                                                                                     <  2:1141261/150‑1 (MQ=255)
                                                              gTCTGAAATCGCTTGAGCGCAAGGTTAATCAACAAGACTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAAGAGTATCTTGac                                                                                   >  2:394929/1‑150 (MQ=255)
                                                              gTCTGAAATCGCTTGAGCGCAAGGTTAATCAACAAGACTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAAGAGTATCTTGac                                                                                   >  2:974140/1‑150 (MQ=255)
                                                              gTCTGAAATCGCTTGAGCGCAAGGTTAATCAACAAGACTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAAGAGTATCTTGaa                                                                                   >  1:589321/1‑149 (MQ=255)
                                                               tCTGAAATCGCTTGAGCGCAAGGTTAATCAACAAGACTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAAGAGTATCTTGact                                                                                  <  2:1018863/150‑1 (MQ=255)
                                                                cTGAAATCGCTTGAGCGCAAGGTTAATCAACAAGACTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAAGAGTATCTTGacta                                                                                 >  2:838356/1‑150 (MQ=255)
                                                                   acagcGCTTGAGCGCAAGGTTAATCAACAAGACAAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAAGAGTATCTTGActacta                                                                              <  2:292045/146‑1 (MQ=255)
                                                                   aaaTCGCTTGAGCGCAAGGTTAATCAACAAGACTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTTTTATTGTCGTTTCTTATATTTTGACAGGAAGAGTATCTTGActacta                                                                              >  2:101049/1‑150 (MQ=255)
                                                                   aaaTCGCTTGAGCGCAAGGTTAATCAACAAGACTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAAGAGTATCTTGActacta                                                                              <  1:272746/150‑1 (MQ=255)
                                                                             aGCGCAAGGTTAATCAACAAGACTTACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAAGAGTATCTTGACTACTAACACTCATAc                                                                    <  2:1313277/150‑1 (MQ=255)
                                                                             aGCGCAAGGTTAATCAACAAGACTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAAGAGTATCTTGACTACTAACACTCATAc                                                                    >  2:416198/1‑150 (MQ=255)
                                                                                   aGGTTAATCAACAAGACTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAAGAGTATCTTGACTACTAACACTCATACTCTGCa                                                              <  1:1064065/150‑1 (MQ=255)
                                                                                    ggTTAATCAACAAGACTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAAGAGTATCTTGGCTACTAACACTCATACTCTGCAg                                                             <  2:1403355/150‑1 (MQ=255)
                                                                                         aTCAACAAGACTAACGTTCCATCTTTTGTTAGCCAAACTTTACGGCCTGTCTCATTCTTACTATTGCTGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTTACAGGAAGAGTATCTAGACTAAAAACACTCATACTATGCAGATTGa                                                        >  2:1531282/1‑150 (MQ=255)
                                                                                           caacaaGACTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAAGAGTATCTTGACTACTAACACTCATACTCTGCAGATTGAag                                                      <  1:123335/150‑1 (MQ=255)
                                                                                           caacaaGACTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAAGAGTATCTTGACTACTAACACTCATACTCTGCAGATTGAag                                                      >  1:1422839/1‑150 (MQ=255)
                                                                                              caaGACTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAAGAGTATCTTGACTACTAACACTCATACTCTGCAGATTGAAgaga                                                   >  1:931104/1‑150 (MQ=255)
                                                                                                   cTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAAGAGTATCTTGACTACTAACACTCATACTCTGCAGATTGAAGAGATTTTa                                              >  2:983498/1‑150 (MQ=255)
                                                                                                       cGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAAGAGTATCTTGACTACTAACACTCATACTCTGCAGATTGAAGAGATTTTAGAAc                                          <  2:183265/150‑1 (MQ=255)
                                                                                                            cATGTTTTGTTCTCCAAAGTTTTCGGCCTGTGTCTTTCTTACGATTCTGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAAGAGTATCTTGACTTCTAACACTCTTACTCTGGAGATTGAAGAGATTTTAGAACTTCTg                                     <  1:228141/150‑1 (MQ=255)
                                                                                                             aTCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAAGAGTATCTTGACTACTAACACTCATACTCTGCAGATTGAAGAGATTTTAGAACTTCTGc                                    >  2:1387812/1‑150 (MQ=255)
                                                                                                               cTTTTGTTCGCCAAACTTTACGTCATGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGGTTCTTATAATTTGACAGGAAGGGTAACTTGACTACTTACTCTGATACTCAGCAGATTGAAGAGATTTTAGAACTTCTGcag                                  >  1:1090165/1‑148 (MQ=255)
                                                                                                               cTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCAGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAAGAGTATCTTGACTACTAACACTGATACTCTGCAGATTGAAGAGATTTTAGAACTTCTGCCg                                  >  1:67867/1‑150 (MQ=255)
                                                                                                                  ttGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAAGAGTATCTTGACTACTAACACTCATACTCTGCAGATTGAAGAGATTTTAGAACTTCTGCCGCAc                               >  1:1588175/1‑150 (MQ=255)
                                                                                                                     ttCGCCAATCTTTACGGCCTTTCTCATTATTACGATTTCGGCAGGCCGTTTTATTATTGTCGTTTCTTATATTTTGACAGGAAGAGTATCTTGACTACTTACACTCATACTCTGGAGATTGAAGAGATTTTAGAACTTGTGCCGCACCGt                            <  2:516131/150‑1 (MQ=255)
                                                                                                                       cGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTGTCTTATATTTTGACAGGAAGAGTATCTTGACTACTAACACTCATACTCTGCAGATTGAAGAGAGTTTAGAACTTCTGCCGCACCGttt                          >  1:892646/1‑150 (MQ=255)
                                                                                                                        gCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAAGAGTATCTTGACTACTAACACTCATACTCTGCAGATTGAAGAGATTTTAGAACTTCTGCCGCACCGTTTc                         <  2:1564698/150‑1 (MQ=255)
                                                                                                                        gCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAAGAGTATCTTGACTACTAACACTCATACTCTGCAGATTGAAGAGATTTTAGAACTTCTGCCGCACCGTTTc                         >  2:64703/1‑150 (MQ=255)
                                                                                                                        gCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAAGAGTATCTTGACTACTAACACTCATACTCTGCAGATTGAAGAGATTTTAGAACTTCTGCCGCACCGTTTc                         >  1:615588/1‑150 (MQ=255)
                                                                                                                         ccAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAAGAGTATCTTGACTACTAACACTCATACTCTGCAGATTGAAGAGATTTTAGAACTTCTGCCGCACCGTTTcc                        >  2:1690401/1‑150 (MQ=255)
                                                                                                                         ccAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAAGAGTATCTTGACTACTAACACTCATACTCTGCAGATTGAAGAGATTTTAGAACTTCTGCCGCACCGTTTcc                        <  1:99517/150‑1 (MQ=255)
                                                                                                                         ccAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAAGAGTATCTTGACTACTAACACTCATACTCTGCAGATTGAAGAGATTTTAGAACTTCTGCCGCACCGTTTcc                        <  2:146159/150‑1 (MQ=255)
                                                                                                                          cAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAAGAGTATCTTGACTACTAACACTCATACTCTGCAGATTGAAGAGATTTTAGAACTTCTGCCGCACCGTTTccc                       <  1:394929/150‑1 (MQ=255)
                                                                                                                          cAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAAGAGTATCTTGACTACTAACACTCATACTCTGCAGATTGAAGAGATTTTAGAACTTCTGCCGCACCGTTTccc                       <  1:974140/150‑1 (MQ=255)
                                                                                                                            aataacaCGGCCTGCCGCAATCGTAAGAATGAGACAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAAGAGTATCTTGACTACTAACACTCATACTCTGCAGATTGAAGAGATTTTAGAACTTCTGCCGCACCGTTTCCCGt                     >  2:1402039/7‑150 (MQ=37)
                                                                                                                               tttACGTCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAAGAGTATCTTGACTACTAACACTCATACTCTGCAGATTGAAGAGATTTTAGAACTTCTGCCGCACCGTTTCCCGTTCt                  <  1:19426/150‑1 (MQ=255)
                                                                                                                               tttACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAAGAGTATCTTGACTACTAACACTCATACTCTGCAGATTGAAGAGATTTTAGAACTTCTGCCGCACCGTTTCCCGTTCt                  <  1:666258/150‑1 (MQ=255)
                                                                                                                                 tACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAAGAGTATCTTGACTACTAACACTCATACTCTGCAGATTGAAGAGATTTTAGAACTTCTGCCGCACCGTTTCCCGTTCTTa                <  1:1123566/150‑1 (MQ=255)
                                                                                                                                 tACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAAGAGTATCTTGACTACTAACACTCATACTCTGCAGATTGAAGAGATTTTAGAACTTCTGCCGCACCGTTTCCCGTTCTTa                <  1:1129374/150‑1 (MQ=255)
                                                                                                                                  aCGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAAGAGTATCTTGACTACTAACACTCATACTCTGCAGATTGAAGAGATTTTAGAACTTCTGCCGCACCGTTTCCCGTTCTTAc               <  2:1072095/150‑1 (MQ=255)
                                                                                                                                    ggCCTGTCTAAATCTTACGATTGCGGCAAGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAAGAGTATCTTGACTACTAACACTCATACTCTGCACATTGAAGAGATTTTAGAACTTCTGACGCACCGTTTCCCTTTATTACtt             >  2:1640879/1‑149 (MQ=255)
                                                                                                                                    ggCCTGCCGCAATCGTAAGAATGAGACAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAAGAGTATCTTGACTACTAACACTCATACTCTGCAGATTGAAGAGATTTTAGAACTTCTGCCGCACCGTTTCCCGTTCTTACtg             >  1:1188441/1‑150 (MQ=255)
                                                                                                                                    ggCCTGCCGCAATCGTAAGAATGAGACAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAAGAGTATCTTGACTACTAACACTCATACTCTGCAGATTGAAGAGATTTTAGAACTTCTGCCGCACCGTTTCCCGTTCTTACtg             >  1:510191/1‑150 (MQ=255)
                                                                                                                                         gTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAAGAGTATCTTGACTACTAACACTCATACTCTGCAGATTGAAGAGATTTTAGAACTTCTGCCGCACCGTTTCCCGTTCTTACTGGTGGa        <  2:1240734/150‑1 (MQ=255)
                                                                                                                                         gTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAAGAGTATCTTGACTACTAACACTCATACTCTGCAGATTGAAGAGATTTTAGAACTTCTGCCGCACCGTTTCCCGTTCTTACTGGTGGa        >  1:38769/1‑150 (MQ=255)
                                                                                                                                           ctcATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAAGAGTATCTTGACTACTAACACTCATACTCTGCAGATTGAAGAGATTTTAGAACTTCTGCCGCACCGTTTCCCGTTCTTACTGGTGGATc      <  2:1635201/150‑1 (MQ=255)
                                                                                                                                            tcATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAAGAGTATCTTGCCTACTAACACTCATACTCTGCAGATTGAAGAGATTTTAGAACTTCTGCCGCACCGTTTCCCGTTCTTACTGGTGGATcg     >  2:838879/1‑150 (MQ=255)
                                                                                                                                            tcATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAAGAGTATCTTGACTACTAACACTCATACTCTGCAGATTGAAGAGATTTTAGAACTTCTGCCGCACCGTTTCCCGTTCTTACTGGTGGATcg     <  1:838356/150‑1 (MQ=255)
                                                                                                                                             cATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAAGAGTATCTTGACTACTAACACTCATACTCTGCAGATTGAAGAGATTTTAGAACTTCTGCCGCACCGTTTCCCGTTCTTACTGGTGGATcgc    >  2:1675582/1‑150 (MQ=255)
                                                                                                                                               ttCTTACGATTGCGGCAGTCCGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAAGAGTATCTTGACTACTAACACTCATACTCTGCAGATTGAAGAGATTTTAGAACTTCTGCCGCACCGTTTCCCGTTCTTACTGGTGGATCGCGt  <  2:262104/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                               
ACCCAACAAAGTCTGGCGCAAAACCGCTGCACTGGTGATGAACATTGATGACATGAGCAAGCGTCTGAAATCGCTTGAGCGCAAGGTTAATCAACAAGACTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAAGAGTATCTTGACTACTAACACTCATACTCTGCAGATTGAAGAGATTTTAGAACTTCTGCCGCACCGTTTCCCGTTCTTACTGGTGGATCGCGT  >  NC_000913/201894‑202186

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: