Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 202,049 C→A 5.1% intergenic (+53/‑52) lpxD → / → fabZ UDP‑3‑O‑(3‑hydroxymyristoyl)‑glucosamine N‑acyltransferase/(3R)‑hydroxymyristol acyl carrier protein dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913202,0490CA 5.1% 326.5 / 4.7 107intergenic (+53/‑52)lpxD/fabZUDP‑3‑O‑(3‑hydroxymyristoyl)‑glucosamine N‑acyltransferase/(3R)‑hydroxymyristol acyl carrier protein dehydratase
Reads supporting (aligned to +/- strand):  ref base C (49/52);  new base A (3/2);  total (52/55)
Fisher's exact test for biased strand distribution p-value = 6.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.42e-01

Only 100 of 109 total aligned reads displayed.

CAAAGTCTGGCGCAAAACCGCTGCACTGGTGATGAACATTGATGACATGAGCAAGCGTCTGAAATCGCTTGAGCGCAAGGTTAATCAACAAGACTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAAGAGTATCTTGACTACTAACACTCATACTCTGCAGATTGAAGAGATTTTAGAACTTCTGCCGCACCGTTTCCCGTTCTTACTGGTGGATCGCGTGCTGGATTTTGA  >  NC_000913/201900‑202198
                                                                                                                                                     |                                                                                                                                                     
caaAGTCTGGCGCAAAACCGCTGCACTGGTGATGAACATTGATGACATGAGCAAGCGTCTGAAATCGCTTGAGCGCAAGGTTAATCAACAAGACTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGc                                                                                                                                                       >  1:799966/1‑150 (MQ=255)
    gTCTGGCGCAAAACCGCTGCACTGGTGATGAACATTGATGACATGAGCAAGCGTCTGAAATCGCTTGAGCGCAAGGTTAATCAACAAGACTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCa                                                                                                                                                   >  2:775391/1‑150 (MQ=255)
        ggCGCAAAACCGCTGCACTGGTGATGAACATTGATGACATGAGCAAGCGTCTGAAATCGCTTGAGCGCAAGGTTAATCAACAAGACTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGcc                                                                                                                                               >  2:272746/1‑150 (MQ=255)
         gcgcAAAACCGCTGCACTGGTGATGAACATTGATGACATGAGCAAGCGTCTGAAATCGCTTGAGCGCAAGGTTAATCAACAAGACTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGCCGCAATCGTAAGAATGAGACAGGCCg                                                                                                                                              <  2:566764/150‑1 (MQ=255)
            cAAAACCGCTGCACTGGTGATGAACATTGATGACATGAGCAAGCGTCTGAAATCGCTTGAGCGCAAGGTTAATCAACAAGACTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGCCGCAATCGTAAGAATGAGACAGGCCgtaa                                                                                                                                           <  2:690344/150‑3 (MQ=38)
              aaaCCGCTGCACTGGTGATGAACATTGATGACATGAGCAAGCGTCTGAAATCGCTTGAGCGCAAGGTTAATCAACAAGACTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGtta                                                                                                                                         <  2:1135410/150‑1 (MQ=255)
               aaCCGCTGCACTGGTGATGAACATTGATGACATGAGCAAGCGTCTGAAATCGCTTGAGCGCAAGGTTAATCAACAAGACTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGttat                                                                                                                                        >  2:67672/1‑150 (MQ=255)
                aCCGCTGCACTGGTGATGAACATTGATGACATGAGCAAGCGTCTGAAATCGCTTGAGCGCAAGGTTAATCAACAAGACTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGttatt                                                                                                                                       >  2:1129374/1‑150 (MQ=255)
                aCCGCTGCACTGGTGATGAACATTGATGACATGAGCAAGCGTCTGAAATCGCTTGAGCGCAAGGTTAATCAACAAGACTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGttatt                                                                                                                                       >  2:1123566/1‑150 (MQ=255)
                  cGCTGCACTGGTGATGAACATTGATGACATGAGCAAGCGTCTGAAATCGCTTGAGTGCAAGGTTAATCAACAAGACTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTtattat                                                                                                                                     <  2:703776/150‑1 (MQ=255)
                    cTGCACTGGTGATGATCATTGATGTCATTAGCAAGCGTCTGAAATCGCTTGAGCGCAAGGGTAATCAACAAGACTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTg                                                                                                                                   <  2:1591327/150‑1 (MQ=255)
                     tGCACTGGTGATGAACATTGATGACATGAGCAAGCGTCTGAAATCGCTTGAGCGCAAGGTTAATCAACAAGACTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGt                                                                                                                                  <  2:1063876/150‑1 (MQ=255)
                     tGCACTGGTGAGGAACATTGATGACATGAGCAAGCGTCTGAAATCGCTTGAGCGCAAGGTTAATCAACAAGACTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGt                                                                                                                                  <  2:1058307/150‑1 (MQ=255)
                      tgaCTGGTGATGAACATTGATGTCATGATCAATCGTCTGAAATGGCTTGAGCGCAATGTTAATCAACAAGTCTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTAAGATTGCGGCAGGCCGTGTTATTATTGTc                                                                                                                                 <  2:1425682/148‑1 (MQ=255)
                      gCACTGGTGATGAACATTGATGACATGAGCAAGCGTCTGAAATCGCTTGAGCGCAAGGTTAATCAACAAGACTAACGTTCCATCTTTTGTTCGCCAAACTATACGGCCTGTCTCATTCTTAAGATTGCGGCAGGCCGTGTTATTATTGTc                                                                                                                                 >  2:955813/1‑150 (MQ=255)
                       cACTGGTGATGAACATTGATGACATGAGCAAGCGTCTGAAATCGCTTGAGCGCAAGGTTAATCAACAAGACTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCg                                                                                                                                >  1:1072095/1‑150 (MQ=255)
                       cACTGGTGATGAACATTGATGACATGAGCAAGCGTCTGAAATCGCTTGAGCGCAAGGTTAATCAACAAGACTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCg                                                                                                                                <  1:1185602/150‑1 (MQ=255)
                       cACTGGTGATGAACATTGATGACATGAGCAAGCGTCTGAAATCGCTTGAGCGCAAGGTTAATCAACAAGACTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCg                                                                                                                                <  1:357426/150‑1 (MQ=255)
                        aCTGGTGATGAACATTGATGACATGAGCAAGCGTCTGAAATCGCTTGAGCGCAAGGTTAATCAACAAGACTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGt                                                                                                                               <  1:961121/150‑1 (MQ=255)
                         cTGGTGATGAACATTGATGACATGAGCAAGCGTCTGAAATCGCTTGAGCGCAAGGTTAATCAACAAGACTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATACTTACGATTGCGGCAGGCCGTGTTATTAATGTCGtt                                                                                                                              >  2:387370/1‑150 (MQ=255)
                           ggTGATGAACATTGATGACATGCGCACGCGTCTGAAATCGCTTGAGCGCAAGGTTAATCCACAAGACTAACGTTCCATCTTTTGTTCGCCAAACTGTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTc                                                                                                                            <  2:224174/150‑1 (MQ=255)
                           ggTGATGAACATTGATGACATGAGCAAGCGTCTGAAATCGCTTGAGCGCAAGGTTAATCAACAAGACTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTc                                                                                                                            <  2:809155/150‑1 (MQ=255)
                             tgatgaACATTGATGACATGAGCAAGCGTCTGAAATCGCTTGAGCGCAAGGTTAATCAACAAGACTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCtt                                                                                                                          <  1:189291/150‑1 (MQ=255)
                              gatgaACATTGATGACATGAGCAAGCGTCTGAAATCGCTTGAGCGCAAGGTTAATCAACAAGACTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTta                                                                                                                         >  1:1388678/1‑150 (MQ=255)
                              gatgaACATTGATGACATGAGCAAGCGTCTGAAATCGCTTGAGCGCAAGGTTAATCAACAAGACTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTta                                                                                                                         >  1:1381880/1‑150 (MQ=255)
                                  aaCATTGATGACATGAGCAAGCGTCTGAAATCGCTTGAGCGCAAGGTTAATCAACAAGACTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGttatta                                                                                                                                      <  1:1152044/133‑1 (MQ=255)
                                  aaCATTGATGACATGAGCAAGCGTCTGAAATCGCTTGAGCGCAAGGTTAATCAACAAGACTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGttatta                                                                                                                                      >  2:1152044/1‑133 (MQ=255)
                                  aaCATTGATGACATGAGCAAGCGTCTGAAATCGCTTGAGCGCAAGGTTAATCAACAAGACTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATAtt                                                                                                                     <  2:1259470/150‑1 (MQ=255)
                                   aCATTGATGACATGAGCAAGCGTCTGAAATCGCTTGAGCGCAAGGTTAATCAACAAGACTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATAttt                                                                                                                    >  2:1527424/1‑150 (MQ=255)
                                    aatttctgaCATTAGCAAGCGGATGAAATCGCTTTAGCTCAATGGTACTCAACAAGAATAACGTTCCATCTTTTGTTCGCCAAAATTTACGTCCTGTATCATTCTTACGATTGCGGCAGGCCGTTTTATTATTTTCGTTTCTTATAtttt                                                                                                                   <  1:126272/144‑1 (MQ=255)
                                     aTTGATGACATGAGCAAGCGTCTGAAATCGCTTGAGCGCAAGGTTAATCAACAAGACTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTg                                                                                                                  <  1:1529157/150‑1 (MQ=255)
                                     aTTGATGACATGAGCAAGCGTCTGAAATCGCTTGAGCGCAAGGTTAATCAACAAGACTAACGTTCCATCTGTTGTACGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTAATAGTGTCGTTTCTTATATTTTg                                                                                                                  >  2:843660/1‑150 (MQ=255)
                                      ttGATGACATGAGCAAGCGTCTGAAATCGCTTGAGCGCAAGGTTAATCAACAAGACTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGa                                                                                                                 <  2:119156/150‑1 (MQ=255)
                                      ttGATGACATGAGCAAGCGTCTGAAATCGCTTGAGCGCAAGGTTAATCAACAAGACTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGa                                                                                                                 <  1:549344/150‑1 (MQ=255)
                                          tgaCATGAGCAAGCGTCTGAAATCGCTTGAGCGCAAGGTTAATCAACAAGACTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAgg                                                                                                             >  1:1035003/1‑150 (MQ=255)
                                          tgaCATGAGCAAGCGTCTGAAATCGCTTGAGCGCAAGGTTAATCAACAAGACTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAgg                                                                                                             <  1:1572417/150‑1 (MQ=255)
                                          tgaCATGAGCAAGCGTCTGAAATCGCTTGAGCGCAAGGTTAATCAACAAGACTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAgg                                                                                                             <  1:319171/150‑1 (MQ=255)
                                           gaCATGAGCAAGCGTCTGAAATCGCTTGAGCGCAAGGTTAATCAACAAGACTAACGTTCCATCTTTTGTTCGCCAAACTTAACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTAATTATTGTCGTTTCTTATATTTTGACAGGa                                                                                                            >  1:260736/1‑150 (MQ=255)
                                             cATGAGCAAGCGTCTGAAATCGCTTGAGCGCAAGGTTAATCAACAAGACTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAag                                                                                                          <  2:542692/150‑1 (MQ=255)
                                               tGAGCAAGCGTCTGAAATCGCTTGAGCGCAAGGTTAATCAACAAGACTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAagag                                                                                                        >  1:1635201/1‑150 (MQ=255)
                                                   cAAGCGTCTGAAATCGCTTGAGCGCAAGGTTAATCAACAAGACTAACGTTCCAACTTTTGTTCGCCAAACTTTACGGCCTGTATCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAAGAGTAta                                                                                                    >  2:1712640/1‑149 (MQ=255)
                                                    aaGCTTCTGAAATCGCTTGATCGCAAGGTTAATCAACAATACTATCGTTCCATCTTTTTTTCTCCAAACTTTACGTCCTGTCTGATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAAGAGTATCt                                                                                                   <  1:287091/150‑1 (MQ=255)
                                                    aaGCGTCTGAAATCGCTTGAGCGCAAGGTTAATCAACAAGACTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAAGAGTATCt                                                                                                   <  2:530852/150‑1 (MQ=255)
                                                     aGCGTCTGAAATCGCTTGAGCGCAAGGTTAATCAACAAGACTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAAGAGTATCtt                                                                                                  >  1:925554/1‑150 (MQ=255)
                                                      gCGTCTGAAATCTCTTGAGCTCAAGGTTAATCAACAATACTAACGTTCTATCTTTTGTTCGCCAAACTTTACGGCCTTTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAAGTGTATCTTg                                                                                                 <  2:1141261/150‑1 (MQ=255)
                                                        gTCTGAAATCGCTTGAGCGCAAGGTTAATCAACAAGACTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAAGAGTATCTTGac                                                                                               >  2:974140/1‑150 (MQ=255)
                                                        gTCTGAAATCGCTTGAGCGCAAGGTTAATCAACAAGACTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAAGAGTATCTTGac                                                                                               >  2:394929/1‑150 (MQ=255)
                                                        gTCTGAAATCGCTTGAGCGCAAGGTTAATCAACAAGACTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAAGAGTATCTTGaa                                                                                               >  1:589321/1‑149 (MQ=255)
                                                         tCTGAAATCGCTTGAGCGCAAGGTTAATCAACAAGACTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAAGAGTATCTTGact                                                                                              <  2:1018863/150‑1 (MQ=255)
                                                          cTGAAATCGCTTGAGCGCAAGGTTAATCAACAAGACTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAAGAGTATCTTGacta                                                                                             >  2:838356/1‑150 (MQ=255)
                                                           tGAAATCACTTGAGCGCAAGGTTAATCAACAAGACTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAAGAGTATCTTGactac                                                                                            <  2:1418525/150‑1 (MQ=255)
                                                             acagcGCTTGAGCGCAAGGTTAATCAACAAGACAAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAAGAGTATCTTGActacta                                                                                          <  2:292045/146‑1 (MQ=255)
                                                             aaaTCGCTTGAGCGCAAGGTTAATCAACAAGACTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAAGAGTATCTTGActacta                                                                                          <  1:272746/150‑1 (MQ=255)
                                                                   cTTGAGCGCAAGGTTAATCAACAAGACTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAAGAGTATCTTGACTACTAACACTc                                                                                    >  2:568648/1‑150 (MQ=255)
                                                                       aGCGCAAGGTTAATCAACAAGACTTACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAAGAGTATCTTGACTACTAACACTCATAc                                                                                <  2:1313277/150‑1 (MQ=255)
                                                                       aGCGCAAGGTTAATCAACAAGACTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAAGAGTATCTTGACTACTAACACTCATAc                                                                                >  2:416198/1‑150 (MQ=255)
                                                                             aGGTTAATCAACAAGACTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAAGAGTATCTTGACTACTAACACTCATACTCTGCa                                                                          <  1:1064065/150‑1 (MQ=255)
                                                                              ggTTAATCAACAAGACTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAAGAGTATCTTGGCTACTAACACTCATACTCTGCAg                                                                         <  2:1403355/150‑1 (MQ=255)
                                                                                     caacaaGACTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAAGAGTATCTTGACTACTAACACTCATACTCTGCAGATTGAag                                                                  <  1:123335/150‑1 (MQ=255)
                                                                                     caacaaGACTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAAGAGTATCTTGACTACTAACACTCATACTCTGCAGATTGAag                                                                  >  1:1422839/1‑150 (MQ=255)
                                                                                        caaGACTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAAGAGTATCTTGACTACTAACACTCATACTCTGCAGATTGAAgaga                                                               >  1:931104/1‑150 (MQ=255)
                                                                                         aaGACTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAAGAGTATCTTGACTACTAACACTCATACTCTGCAGATTGAAGAGAt                                                              >  2:206470/1‑150 (MQ=255)
                                                                                          aGACTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAAGAGTATCTTGACTACTAACACTCATACTCTGCAGATTGAAGAGAtt                                                             >  2:1098198/1‑150 (MQ=255)
                                                                                             cTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAAGAGTATCTTGACTACTAACACTCATACTCTGCAGATTGAAGAGATTTTa                                                          >  2:983498/1‑150 (MQ=255)
                                                                                                 cGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAAGAGTATCTTGACTACTAACACTCATACTCTGCAGATTGAAGAGATTTTAGAAc                                                      <  2:183265/150‑1 (MQ=255)
                                                                                                      cATGTTTTGTTCTCCAAAGTTTTCGGCCTGTGTCTTTCTTACGATTCTGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAAGAGTATCTTGACTTCTAACACTCTTACTCTGGAGATTGAAGAGATTTTAGAACTTCTg                                                 <  1:228141/150‑1 (MQ=255)
                                                                                                       aTCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAAGAGTATCTTGACTACTAACACTCATACTCTGCAGATTGAAGAGATTTTAGAACTTCTGc                                                >  2:1387812/1‑150 (MQ=255)
                                                                                                         cTTTTGTTCGCCAAACTTTACGTCATGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGGTTCTTATAATTTGACAGGAAGGGTAACTTGACTACTTACTCTGATACTCAGCAGATTGAAGAGATTTTAGAACTTCTGcag                                              >  1:1090165/1‑148 (MQ=255)
                                                                                                         cTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCAGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAAGAGTATCTTGACTACTAACACTGATACTCTGCAGATTGAAGAGATTTTAGAACTTCTGCCg                                              >  1:67867/1‑150 (MQ=255)
                                                                                                            ttGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAAGAGTATCTTGACTACTAACACTCATACTCTGCAGATTGAAGAGATTTTAGAACTTCTGCCGCAc                                           >  1:1588175/1‑150 (MQ=255)
                                                                                                               ttCGCCAATCTTTACGGCCTTTCTCATTATTACGATTTCGGCAGGCCGTTTTATTATTGTCGTTTCTTATATTTTGACAGGAAGAGTATCTTGACTACTTACACTCATACTCTGGAGATTGAAGAGATTTTAGAACTTGTGCCGCACCGt                                        <  2:516131/150‑1 (MQ=255)
                                                                                                                 cGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTGTCTTATATTTTGACAGGAAGAGTATCTTGACTACTAACACTCATACTCTGCAGATTGAAGAGAGTTTAGAACTTCTGCCGCACCGttt                                      >  1:892646/1‑150 (MQ=255)
                                                                                                                  gCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAAGAGTATCTTGACTACTAACACTCATACTCTGCAGATTGAAGAGATTTTAGAACTTCTGCCGCACCGTTTc                                     <  2:1564698/150‑1 (MQ=255)
                                                                                                                  gCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAAGAGTATCTTGACTACTAACACTCATACTCTGCAGATTGAAGAGATTTTAGAACTTCTGCCGCACCGTTTc                                     >  1:615588/1‑150 (MQ=255)
                                                                                                                   ccAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAAGAGTATCTTGACTACTAACACTCATACTCTGCAGATTGAAGAGATTTTAGAACTTCTGCCGCACCGTTTcc                                    <  2:146159/150‑1 (MQ=255)
                                                                                                                   ccAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAAGAGTATCTTGACTACTAACACTCATACTCTGCAGATTGAAGAGATTTTAGAACTTCTGCCGCACCGTTTcc                                    >  2:1690401/1‑150 (MQ=255)
                                                                                                                   ccAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAAGAGTATCTTGACTACTAACACTCATACTCTGCAGATTGAAGAGATTTTAGAACTTCTGCCGCACCGTTTcc                                    <  1:99517/150‑1 (MQ=255)
                                                                                                                    cAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAAGAGTATCTTGACTACTAACACTCATACTCTGCAGATTGAAGAGATTTTAGAACTTCTGCCGCACCGTTTccc                                   <  1:974140/150‑1 (MQ=255)
                                                                                                                    cAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAAGAGTATCTTGACTACTAACACTCATACTCTGCAGATTGAAGAGATTTTAGAACTTCTGCCGCACCGTTTccc                                   <  1:394929/150‑1 (MQ=255)
                                                                                                                      aataacaCGGCCTGCCGCAATCGTAAGAATGAGACAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAAGAGTATCTTGACTACTAACACTCATACTCTGCAGATTGAAGAGATTTTAGAACTTCTGCCGCACCGTTTCCCGt                                 >  2:1402039/7‑150 (MQ=37)
                                                                                                                         tttACGTCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAAGAGTATCTTGACTACTAACACTCATACTCTGCAGATTGAAGAGATTTTAGAACTTCTGCCGCACCGTTTCCCGTTCt                              <  1:19426/150‑1 (MQ=255)
                                                                                                                         tttACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAAGAGTATCTTGACTACTAACACTCATACTCTGCAGATTGAAGAGATTTTAGAACTTCTGCCGCACCGTTTCCCGTTCt                              <  1:666258/150‑1 (MQ=255)
                                                                                                                           tACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAAGAGTATCTTGACTACTAACACTCATACTCTGCAGATTGAAGAGATTTTAGAACTTCTGCCGCACCGTTTCCCGTTCTTa                            <  1:1129374/150‑1 (MQ=255)
                                                                                                                           tACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAAGAGTATCTTGACTACTAACACTCATACTCTGCAGATTGAAGAGATTTTAGAACTTCTGCCGCACCGTTTCCCGTTCTTa                            <  1:1123566/150‑1 (MQ=255)
                                                                                                                            aCGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAAGAGTATCTTGACTACTAACACTCATACTCTGCAGATTGAAGAGATTTTAGAACTTCTGCCGCACCGTTTCCCGTTCTTAc                           <  2:1072095/150‑1 (MQ=255)
                                                                                                                             cGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAAGAGTAGCTTGACTACTAACACTCATTCTCTGCAGATTGAAGAGATTTTAGAACTTCTGCCGCTCCGTTTCCCGTTCTGACt                          >  1:269419/1‑150 (MQ=255)
                                                                                                                              ggCCTGTCTAAATCTTACGATTGCGGCAAGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAAGAGTATCTTGACTACTAACACTCATACTCTGCACATTGAAGAGATTTTAGAACTTCTGACGCACCGTTTCCCTTTATTACtt                         >  2:1640879/1‑149 (MQ=255)
                                                                                                                              ggCCTGCCGCAATCGTAAGAATGAGACAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAAGAGTATCTTGACTACTAACACTCATACTCTGCAGATTGAAGAGATTTTAGAACTTCTGCCGCACCGTTTCCCGTTCTTACtg                         >  1:510191/1‑150 (MQ=255)
                                                                                                                              ggCCTGCCGCAATCGTAAGAATGAGACAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAAGAGTATCTTGACTACTAACACTCATACTCTGCAGATTGAAGAGATTTTAGAACTTCTGCCGCACCGTTTCCCGTTCTTACtg                         >  1:1188441/1‑150 (MQ=255)
                                                                                                                                   gTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAAGAGTATCTTGACTACTAACACTCATACTCTGCAGATTGAAGAGATTTTAGAACTTCTGCCGCACCGTTTCCCGTTCTTACTGGTGGa                    >  1:38769/1‑150 (MQ=255)
                                                                                                                                   gTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAAGAGTATCTTGACTACTAACACTCATACTCTGCAGATTGAAGAGATTTTAGAACTTCTGCCGCACCGTTTCCCGTTCTTACTGGTGGa                    <  2:1240734/150‑1 (MQ=255)
                                                                                                                                     ctcATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAAGAGTATCTTGACTACTAACACTCATACTCTGCAGATTGAAGAGATTTTAGAACTTCTGCCGCACCGTTTCCCGTTCTTACTGGTGGATc                  <  2:1635201/150‑1 (MQ=255)
                                                                                                                                      tcATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAAGAGTATCTTGCCTACTAACACTCATACTCTGCAGATTGAAGAGATTTTAGAACTTCTGCCGCACCGTTTCCCGTTCTTACTGGTGGATcg                 >  2:838879/1‑150 (MQ=255)
                                                                                                                                      tcATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAAGAGTATCTTGACTACTAACACTCATACTCTGCAGATTGAAGAGATTTTAGAACTTCTGCCGCACCGTTTCCCGTTCTTACTGGTGGATcg                 <  1:838356/150‑1 (MQ=255)
                                                                                                                                       cATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAAGAGTATCTTGACTACTAACACTCATACTCTGCAGATTGAAGAGATTTTAGAACTTCTGCCGCACCGTTTCCCGTTCTTACTGGTGGATcgc                >  2:1675582/1‑150 (MQ=255)
                                                                                                                                         ttCTTACGATTGCGGCAGTCCGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAAGAGTATCTTGACTACTAACACTCATACTCTGCAGATTGAAGAGATTTTAGAACTTCTGCCGCACCGTTTCCCGTTCTTACTGGTGGATCGCGt              <  2:262104/150‑1 (MQ=255)
                                                                                                                                                  ttGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAAGAGTATCTTGACTACTAACACTCATACTCTGCAGATTGAAGAGATTTTAGAACTTCTGCCGCACCGTTTCCCGTTCTTACTGGTGGATCGCGTGCTGGAttt     <  2:1302713/150‑1 (MQ=255)
                                                                                                                                                   tGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAAGAGTATCTTGACTACTAACACTCATACTCTGCAGATTGAAGAGATTTTAGAACTTCTGCCGCACCGTTTCCCGTTCTTACTGGTGGATCGCGTGCTGGAtttt    >  2:447553/1‑150 (MQ=255)
                                                                                                                                                   tGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAAGAGTATCTTGACTACTAACACTCATACTCTGCAGATTGAAGAGATTTTAGAACTTCTGCCGCACCGTTTCCCGTTCTTACTGGTGGATCGCGTGCTGGAtttt    <  1:1098198/150‑1 (MQ=255)
                                                                                                                                                     cGGCAGGCCGTGTTTTTATTGTCGTTTCTTATATTTTGACAGGAAGAGTTTCTTGACTACTAACACTCATACTCTGCAGATTGTAGAGATTTTAGTACTTCTGCCGCACCGTTTCCCGTTCTTACTGGTGGATCGCGTGCTGGATTTTga  <  1:387370/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CAAAGTCTGGCGCAAAACCGCTGCACTGGTGATGAACATTGATGACATGAGCAAGCGTCTGAAATCGCTTGAGCGCAAGGTTAATCAACAAGACTAACGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTCTTATATTTTGACAGGAAGAGTATCTTGACTACTAACACTCATACTCTGCAGATTGAAGAGATTTTAGAACTTCTGCCGCACCGTTTCCCGTTCTTACTGGTGGATCGCGTGCTGGATTTTGA  >  NC_000913/201900‑202198

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: