Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,218,517 A→C 6.3% L14R (CTG→CGG) ‡ yehY ← putative ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,218,5170AC 6.3% 311.1 / 8.4 107L14R (CTG→CGG) ‡yehYputative ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base A (52/48);  new base C (5/2);  total (57/50)
Fisher's exact test for biased strand distribution p-value = 4.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.62e-01

Only 100 of 116 total aligned reads displayed.

CAGGCCGTCAGCCAGGCGCAACCAACGCCCACCAGCATCCAGATCGTTTGCGGCCACAGCTGCCAGAGATGACGCCCCTCACCCGAAACTAAACGATTAGGCGCGTAACTGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGCGCCAGAACAGGATTAATACGGAAATAAGTCACGGGAAATTACTTCGTCCACCCTTTTTGTTTCAGGTAGTCGGCAGCCACTTTTTTGGCATCCAGTCCTTCCACAGCAATGCTGGCATTCAGTTGCTGCAATGTTTTTGC  >  NC_000913/2218370‑2218665
                                                                                                                                                   |                                                                                                                                                    
cAGGCCGTCAGCCAGGCGCAACCAACGCCCACCAGCATCCAGATCGTTTGCGGCCACAGCTGCCAGAGATGACGCCCCTCACCCGAAACTAAACGATTAGGCGCGTAACTGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAAcagc                                                                                                                                                    <  2:1152610/150‑1 (MQ=255)
  ggCCGTCAGCCAGGCGCAACCAACGCCCACCAGCATCCAGATCGTTTGCGGCCACAGCTGCCAGAGATGACGCCCCTCACCCGAAACTAAACGATTAGGCGCGTAACTGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAAcagcag                                                                                                                                                  <  1:10254/150‑1 (MQ=255)
    ccGTCAGCCAGGCGCAACCAACGCCCACCCGCATCCAGATCGTTTGCGGCCACAGCTGACAGAGATGACGCCCCTCACCCGAAACTAAACGATTAGGCGCGTAACTGATAACCGGCAGCGCCGCTGCGATTGCCGTCAGCACcagcagca                                                                                                                                                <  2:924017/150‑1 (MQ=255)
       tCAGCCAGGCGCAACCAACGCCCACCAGCATCCAGATCGTTTGCGGCCACAGCTGCCAGAGATGACGCCCCTCACCCGAAACTAAACGATTAGGCGCGTAACTGATAAACGGCAGCGCTGCTGCTGTTGCTGACGGCAATCGCAgcggcg                                                                                                                                             <  2:1672783/150‑1 (MQ=38)
         aGCCAGGCGCAACCAACGCCCACCAGCATCCAGATCGGTTGCGGCCACAGCTGCCAGAGATGACGCCCCTCACCCGAAACTAAACGATTAGGCGCGTAACTGATAAACGGCAGTGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGCGcc                                                                                                                                           <  1:172792/150‑1 (MQ=255)
          gCCAGGCGCAACCAACGCCCACCAGCATCCAGATCGTTTGCGGCCACAGCTGCCAGAGATGACGCCCCTCACCCGAAACTAAACGATTAGGCGCGTAACTGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGCGCCa                                                                                                                                          >  1:1035712/1‑150 (MQ=255)
          gCCAGGCGCAACCAACGCCCACCAGCATCCAGATCGTTTGCGCCACAGCTGCCAGAGATGACGCCCCTCACCCGAAACTAAACGATTAGGCGCGTAACTGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGCGCCAg                                                                                                                                         >  1:1360416/1‑150 (MQ=255)
           ccAGGCGCAACCAACGCCCACCAGCATCCAGATCGTTTGCGGCCACAGCTGCCAGAGATGACGCCCCTCACCCGAAACTAAACGATTAGGCGCGTAACTGATAAACGGCAGCGCTGCTGCTGTTGCTGACGGCAATCGCAGCGgcgctgc                                                                                                                                         <  2:140717/150‑4 (MQ=38)
                cgcAACCAACGCCCACCAGCATCCAGATCGTTTGCGGCCACAGCTGCCAGAGATGACGCCCCTCACCCGAAACTAAACGATTAGGCGCGTAACTGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGCGCCAGAACAg                                                                                                                                    >  1:1072971/1‑150 (MQ=255)
                 gcAACCAACGCCCACCAGCATCCAGATCGTTTGCGGCCACAGCTGCCAGAGATGACGCCCCTCCCCCGAAACTAAACGATTAGGCGCGTAACTGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGCGCCAGAACAgg                                                                                                                                   >  2:1263221/1‑150 (MQ=255)
                   aaccaacGCCCACCAGCATCCAGATCGTTTGCGGCCACAGCTGCCAGAGATGACGCCCCTCACCCGAAACTAAACGATTAGGCGCGTAACTGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGCGCCAGAACAGGAt                                                                                                                                 >  1:523315/1‑150 (MQ=255)
                        acGCCCACCAGCATCAGATCGTTTGCGGCCACAGCTGCCAGAGATGACGCCCCTCACCCGAAACTAAACGATTAGGCGCGTAACTGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGCGCCAGAACAGGATTAATAc                                                                                                                           <  2:1559167/150‑1 (MQ=255)
                         cGCCCACCAGCATCCAGATCGTTTGCGGCCACAGCTGCCAGAGATGACGCCCCTCACCCGAAACTAAACGATTAGGCGCGTAACTGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGCGCCAGAACAGGATTAATAc                                                                                                                           >  2:1460420/1‑150 (MQ=255)
                          gCCCACCAGCATCCAGATCGTTTGCGCCCACAGCTGCCAGAGATGACGCCCCTCACCCGAAACTAAACGATTAGGCGCGTAACTGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGCGCCAGAACAGGATTAATACg                                                                                                                          <  2:220859/150‑1 (MQ=255)
                             caccaGCATCCAGATCGTTTGCGGCCACAGCTGCCAGAGATGACGCCCCTCACCCGAAACTAAACGATTAGGCGCGTAACTGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGCGCCAGAACAGGATTAATACGGaa                                                                                                                       >  1:1313153/1‑150 (MQ=255)
                               ccaGCATCCAGATCGTTTGCGGCCACAGCTGCCAGAGATGACGCCCCTCACCCGAAACTAAACGATTAGGCGCGTAACTGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGCGCCAGAACAGGATTAATACGGAAAt                                                                                                                     <  2:1017008/150‑1 (MQ=255)
                                caGCATCCAGATCGTTTGCGGCCACAGCTGCCAGAGATGACGCCCCTCACCCGAAACTAAACGATTAGGCGCGTAACTGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAAAAGCAGCAGCGCCAGAACAGGATTAATACGGAAATa                                                                                                                    <  1:1486793/150‑1 (MQ=255)
                                 aGCATCCAGATCGTTTGCGGCCACAGCTGCCAGAGATGACGCCCCTCACCCGAAACTAAACGATTAGGCGCGTAACTGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACagcagcagc                                                                                                                                              <  2:701919/123‑1 (MQ=255)
                                 aGCATCCAGATCGTTTGCGGCCACAGCTGCCAGAGATGACGCCCCTCACCCGAAACTAAACGATTAGGCGCGTAACTGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACagcagcagc                                                                                                                                              >  1:701919/1‑123 (MQ=255)
                                 aGCATCCAGATCGTTTGCGGCCACAGCTGCCAGAGATGACGCCCCTCACCCGAAACTAAACGATTAGGCGCGTAACTGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGCGCCAGAACAGGATTAATACGGAAATaa                                                                                                                   >  2:891419/1‑150 (MQ=255)
                                  gCATCCAGATCGTTTGCGGCCACAGCTGCCAGAGATGACGCCCCTCACCCGAAACTAAACGATTAGGCGCGTAACTGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGCGCCAGAACAGGATTAATACGGAAATAAg                                                                                                                  <  2:1533331/150‑1 (MQ=255)
                                   cATCCAGATCGTTTGCGGCCACAGCTGCCAGAGATGACGCCCCTCACCCGAAACTAAACGATTAGGCGCGTAACTGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGCGCCAGAACAGGATTAATACGGAAATAAGt                                                                                                                 >  1:1387823/1‑150 (MQ=255)
                                      ccAGATCGTTTGCGGCCACAGCTGCCAGAGATGACGCCCCTCACCCGAAACTAAACGATTAGGCGCGTAACTGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGCGCCAGAACAGGATTAATACGGAAATAAGTCAc                                                                                                              >  2:1183631/1‑150 (MQ=255)
                                       cAGATCGTTTGCGGCCACAGCTGCCAGAGATGACGCCCCTCACCCGAAACTAAACGATTAGGCGCGTAACTGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGCGCCAGAACAGGATTAATACGGAAATAAGTCACg                                                                                                             >  1:427790/1‑150 (MQ=255)
                                        aGATCGTTTGCGGCCACAGCTGCCAGAGATGACGCCCCTCACCCGAAACTAAACGATTAGGCGCGTAACTGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGCGCCAGAACAGGATTAATACGGAAATAAGTCACgg                                                                                                            >  1:1256561/1‑150 (MQ=255)
                                        aGATCGTTTGCGGCCACAGCTGCCAGAGATGACGCCCCTCACCCGAAACTAAACGATTAGGCGCGTAACTGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGCGCCAGAACAGGATTAATACGGAAATAAGTCACgg                                                                                                            <  1:1099295/150‑1 (MQ=255)
                                        aGATCGTTTGCGGCCACAGCTGCCAGAGATGACGCCCCTCACCCGAAACTAAACGATTAGGCGCGTAACTGAAAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGCGCCAGAACAGGATTAATACGGAAATAAGTCACgg                                                                                                            >  2:5975/1‑150 (MQ=255)
                                               ttGCGGCCACAGCTGCCAGAGATGACGCCCCTCACCCGAAACTAAACGATTAGGCGCGTAACTGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGCGCCAGAACAGGATTAATACGGAAATAAGTCACGGGAAATTa                                                                                                     <  2:1713124/150‑1 (MQ=255)
                                                tGCGGCCACAGCTGCCAGAGATGACGCCCCTCACCCGAAACTAAACGATTAGGCGCGTAACTGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGCGCCAGCACAGGATAAATACGGAAATAAGTCACGGGAACTTAc                                                                                                    >  2:1627214/1‑150 (MQ=255)
                                                tGCGGCCACAGCTGCCAGAGATGACGCCCCTCACCCGAAACTAAACGATTAGGCGCGTAACTGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGCGCCAGAACAGGATTAATACGGAAATAAGTCACGGGAAATTAc                                                                                                    >  2:752375/1‑150 (MQ=255)
                                                 gCGGCCACAGCTGCCAGAGATGACGCCCCTCACCCGAAACTAAACGATTAGGCGCGTAACTGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGCGCCAGAACAGGATTAATACGGAAATAAGTCACGGGAAATTACt                                                                                                   <  1:1228931/150‑1 (MQ=255)
                                                  gcgCCACAGCTGCCAGAGATGACGCCCCTCACCCGAAACTAAACGATTAGGCGCGTAACTGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGCGCCAGAACAGGATTAATACGGAAATAAGTCACGGGAAATTACtt                                                                                                  <  2:1360416/148‑1 (MQ=255)
                                                   ggCCACAGCTGCCAGAGATGACGCCCCTCACCCGAAACTAAACGATTAGGCGCGTAACTGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGCGCCAGAACAGGATTAATACGGAAATAAGTCACGGGAAATTACTTc                                                                                                 <  2:867016/150‑1 (MQ=255)
                                                   ggCCACAGCTGCCAGAGATGACGCCCCTCACCCGAAACTAAACGATTAGGCGCGTAACTGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGCGCCAGAACAGGATTAATACGGAAATAAGTCACGGGAAATTACTTc                                                                                                 >  2:1535743/1‑150 (MQ=255)
                                                   ggCCACAGCTGCCAGAGATGACGCCCCTCACCCGAAACTAAACGATTAGGCGCGTAACTGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGCGCCAGAACAGGATTAATACGGAAATAAGTCACGGGAAATTACTTc                                                                                                 <  1:863549/150‑1 (MQ=255)
                                                        caGCTGCCAGAGATGACGCCCCTCACCCGAAACTAAACGATTAGGCGCGTAACTGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGCGCCAGAACAGGATTAATACGGAAATAAGTCACGGGAAATTACTTCGTCCa                                                                                            <  1:1128404/150‑1 (MQ=255)
                                                          gCTGCCAGAGATGACGCCCCTCACCCGAAACTAAACGATTAGGCGCGTAACTGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGCGCCAGAACAGGATTAATACGGAAATAAGTCACGGGAAATTACTTCGTCCAcc                                                                                          >  2:633900/1‑150 (MQ=255)
                                                           cTGCCAGAGATGACGCCCCTCACCCGAAACTAAACGATTAGGCGCGTAACTGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGCGCCAGAACAGGATTAATACGGAAATAAGTCACGGGAAATTACTTCGTCCAccc                                                                                         >  1:9313/1‑150 (MQ=255)
                                                             gCCAGAGATGACGCCCCTCACCCGAAACTAAACGATTAGGCGCGTAACTGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGCGCCAGAACAGGATTAATACGGAAATAAGTCACGGGAAATTACTTCGTCCACCCtt                                                                                       <  2:1072971/150‑1 (MQ=255)
                                                                   taTGACGCCCCTCACCCGAAACTAAACGATTAGGCTCGTAACTGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGCGCCAGAACAGGATTAATACGGAAATAAGTCACGGGAAATTACTTCGTCCACCCTTTTTGtt                                                                                 <  1:1308827/149‑1 (MQ=255)
                                                                   gaTGACGCCCCTCACCCGAAACTAAACGATTAGGCGCGTAACTGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGCGCCAGAACAGGATTAATACGGAAATAAGTCACGGGAAATTACTTCGTCCACCCTTTTTGtt                                                                                 <  1:1567753/150‑1 (MQ=255)
                                                                   gaTGACGCCCCTCACCAGAAACTAAACGATTAGTCGCGTAACTGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGCGCCAGTACAGGATTAATACGGAAATAAGTCACGGGAAATTACTTCGTCCACCCTTTTTGtt                                                                                 <  1:256797/150‑1 (MQ=255)
                                                                      gACGCCCCTCACCCGAAACTAAACGATTAGGCGCGTAACTGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGCGCCAGAACAGGATTAATACGGAAATAAGTCACGGGAAATTACTTCGTCCACCCTTTTTGTTTCa                                                                              >  2:1154780/1‑150 (MQ=255)
                                                                        ctccccTCACCCGAAACTAAACGATTAGGCGCGTAACTGATAAACGGCCGCGCCGCTGCGATTGCCGTCAGCTCCCGCAGCAGCGCCCGCACAGGATTAATACGGAAATACGTCACGGGAAATTACTACGTCCCCCCTTTTTGTTACAgg                                                                            >  1:46744/3‑150 (MQ=255)
                                                                        cGCCCCTCACCCGAAACTAAACGATTAGGCGCGTAACTGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGCGCCAGAACAGGATTAATACGGAAATAAGTCACGGGAAATTACTTCGTCCACCCTTTTTGTTTCAgg                                                                            >  2:1161152/1‑150 (MQ=255)
                                                                        cGCCCCTCACCCGAAACTAAACGATTAGGCGCGTAACTGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGCGCCAGAACAGGATTAATACGGAAATAAGTCACGGGAAATTACTTCGTCCACCCTTTTTGTTTCAgg                                                                            <  1:800299/150‑1 (MQ=255)
                                                                         gCCCCTCACCCGAAACTAACCGATTAGGCGCGTAACTGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGCGCCAGAACAGGATTAATACGGAAATAAGTCACGGGAAATTACTTCGTCCACCCTTTTTGTTTCAGGt                                                                           <  2:68394/150‑1 (MQ=255)
                                                                         gCCCCTCACCCGAAACTAAACGATTAGGCGCGTAACTGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGCGCCAGAACAGGATTAATACGGAAATAAGTCACGGGAAATTACTTCGTCCACCCTTTTTGTTTCAGGt                                                                           <  1:119008/150‑1 (MQ=255)
                                                                         gCCCCTCACCCGAAACTAAACGATTAGGCGCGTAACTGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGCGCCAGAACAGGATTAATACGGAAATAAGTCACGGGAAATTACTTCGTCCACCCTTTTTGTTTCAGGt                                                                           <  1:785626/150‑1 (MQ=255)
                                                                             cTCACCCGAAACTAAACGATTAGGCGCGTAACTGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGCGCCAGAACAGGATTAATACGGAAATAAGTCACGGGAAATTACTTCGTCCACCCTTTTTGTTTCAGGTAGTc                                                                       <  1:1460420/150‑1 (MQ=255)
                                                                                aCCCGAAACTAAACGATTAGGCGCGTAACTGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGCGCCAGAACAGGATTAATACGGAAATAAGTCACGGGAAATTACTTCGTCCACCCTTTTTGTTTCAGGTAGTCGGc                                                                    >  2:494540/1‑150 (MQ=255)
                                                                                 cccGAAACTAAACGATTAGGCGCGTAACTGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGCGCCAGAACAGGATTAATACGGAAATAAGTCACGGGAAATTACTTCGTCCACCCTTTTTg                                                                                   >  1:1092835/1‑134 (MQ=255)
                                                                                 cccGAAACTAAACGATTAGGCGCGTAACTGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGCGCCAGAACAGGATTAATACGGAAATAAGTCACGGGAAATTACTTCGTCCACCCTTTTTg                                                                                   <  2:1092835/134‑1 (MQ=255)
                                                                                 cccGAAACTAAACGATTAGGCGCGTAACTGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGCGCCAGAACAGGATTAATACGGAAATAAGTCACGGGAAATTACTTCGTCCACCCTTTTTGTTTCAGGTAGTCGGCa                                                                   >  1:918515/1‑150 (MQ=255)
                                                                                  ccGAAACTAAACGATTAGGCGCGTAACTGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGCGCCAGAACAGGATTAATACGGAAATAAGTCACGGGAAATTACTTCGTCCACCCTTTTTGTTTCAGGTAGTCGGCAg                                                                  >  2:556232/1‑150 (MQ=255)
                                                                                   cGAAACTAAACGATTAGGCGCGTAACTGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGCGCCAGAACAGGATTAATACGGAAATAAGTCACGGGAAATTACTTCGTCCACCCTTTTTGTTTCAGGTAGTCGGCAGc                                                                 >  1:431477/1‑150 (MQ=255)
                                                                                            aCGATTAGGCGCGTAACTGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGCGCCAGAACAGGATTAATACGGAAATAAGTCACGGGAAATTACTTCGTCCACCCTTTTTGTTTCAGGTAGTCGGCAGCCACtttttt                                                        >  1:202229/1‑150 (MQ=255)
                                                                                             cGATTAGGCGCGTAACTGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGCGCCAGAACAGGATTAATACGGAAATAAGTCACGGGAAATTACTTCGTCCACCCTTTTTGTTTCAGGTAGTCGGCAGCCACTTTTTTg                                                       >  2:941032/1‑150 (MQ=255)
                                                                                                ttACGCGCGTCACTGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGCGCCAGAACAGGATTAATACGGAAATAAGTCTCGGGAAATTCCTGCGTCCACCCTTTTTGTTGCAGGTAGGCGGCAGCCACTTTTTTGGCa                                                    <  2:46744/150‑1 (MQ=255)
                                                                                                  aGGCGCGTAACTGATAAACGGCCGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGCGCCAGAACAGGATTAATACGGAAATAAGTCACGGGAAATTACTTCGTCCACCCTTTTTGTTTCAGGTAGTCGGCAGCCACTTTTTTGGCATc                                                  <  1:1440355/150‑1 (MQ=255)
                                                                                                  aGGCGCGTAACTGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGCGCCAGAACAGGATTAATACGGAAATAAGTCACGGGAAATTACTTCGTCCACCCTTTTTGTTTCAGGTAGTCGGCAGCCACTTTTTTGGCATc                                                  >  1:470393/1‑150 (MQ=255)
                                                                                                   ggCGCGTAACTGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGCGCCAGAACAGGATTACTACGGAAATAAGTCCCGGGAAATTACTTCGTCCACCCTTTTTGTTTCAGGTAGTCGGCAGCCACTTTTTTGGCATcc                                                 <  1:1299375/150‑1 (MQ=255)
                                                                                                       cgTAACTGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGCGCCAGAACAGGATTAATACGGAAATAAGTCACGGGAAATTACTTCGTCCACCCTTTTTGTTTCAGGTAGTCGGCAGCCACTTTTTTGGCATCCAGTc                                             <  1:1183631/150‑1 (MQ=255)
                                                                                                         tAACTGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGCGCCAGAACAGGATTAATACGGAAATAAGTCACGGGAAATTACTTCGTCCACCCTTTTTGTTTCAGGTAGTCGGCAGCCACTTTTTTGGCATCCAGTCCt                                           <  1:941032/150‑1 (MQ=255)
                                                                                                         tAACTGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGCGCCAGAACAGGATTAATACGGAAATAAGTCACGGGAAATTACTTCGTCCACCCTTTTTGTTTCAGGTAGTCGGCAGCCACTTTTTTGGCATCCAGTCCt                                           <  1:633900/150‑1 (MQ=255)
                                                                                                         tAACTGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGCGCCAGAACAGGATTAATACGGAAATAAGTCACGGGAAATTACTTCGTCCACCCTTTTTGTTTCAGGTAGTCGGCAGCCACTTTTTTGGCATCCAGTCCt                                           <  2:427790/150‑1 (MQ=255)
                                                                                                         tAACTGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGCGCCAGAACAGGATTAATACGGAAATAAGTCACGGGAAATTACTTCGTCCACCCTTTTTGTTTCAGGTAGTCGGCAGCCACTTTTTTGGCATCCAGTCCt                                           >  1:1322719/1‑150 (MQ=255)
                                                                                                            cTGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGCGCCAGAACAGGATTAATACGGAAATAAGTCACGGGAAATTACTTCGTCCACCCTTTTTGTTTCAGGTAGTCGGCAGCCACTTTTTTGGCATCCAGTCCTTcc                                        <  2:439101/150‑1 (MQ=255)
                                                                                                              gATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGCGCCAGAACAGGATTAATACGGAAATAAGTCACGGGAAATTACTTCGTCCACCCTTTTTGTTTCAGGTAGTCGGCAGCCACTTTTTTGGCATCCAGTCCTTCcac                                      >  1:951521/1‑150 (MQ=255)
                                                                                                                 aaccGGCAGCGCCGCTGCGATTGACGTCAGCAACAGCAGAAGCGACAGAAAAGGATTAATACGGAAATAAGTCACTGGAAATTACTTAGTCCACCCTTTTTGTTTCAGGTTGTCGGCAGCCACTTTTTTGGCATCCAGTCCATCCACAGc                                   <  2:944021/147‑1 (MQ=255)
                                                                                                                  aaCGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGCGCCAGAACAGGATTAATACGGAAATAAGTCACGGGAAATTACTTCGTCCACCCTTTTTGTTTCAGGTAGTCGGCAGCCACTTTTTTGGCATCCAGTCCTTCCACAGCa                                  >  1:618946/1‑150 (MQ=255)
                                                                                                                   aCGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGCGCCAGAACAGGATTAATACGGAAATAAGTCACGGGAAATTACTTCGTCCACCCTTTTTGTTTCAGGTAGTCGGCAGCCACTTTTTTGGCATCCAGTCCTTCCACAGCaa                                 >  1:1180915/1‑150 (MQ=255)
                                                                                                                     ggCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGCGCCAGAACAGGATTAATACGGAAATAAGTCACGGGAAATTACTTCGTCCACCCTTTTTGTTTCAGGTAGTCGGCAGCCACTTTTTTGGCATCCAGTCCTTCCACAGCAATg                               <  1:494540/150‑1 (MQ=255)
                                                                                                                     ggCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGCGCCAGAACAGGATTAATACGGAAATAAGTCACGGGAAATTACTTAGTCCACCCTTTTTGTTTAAGGTAGTCGGCAGCCACTTTTTTGGCATCCAGTCCTTCCACAGCAATg                               >  2:409312/1‑150 (MQ=255)
                                                                                                                      ctggcgcTGCTGCTGTTGCTGACGGCAATCGCAGCGGCGCCAGAACAGGATTAATACGGAAATAAGTCACGGGAAATTACTTCGTCCACCCTTTTTGTTTCAGGTAGTCGGCAGCCACTTTTTTGGCATCCAGTCCTTCCACAGCAATGc                              >  1:1548116/4‑150 (MQ=38)
                                                                                                                       cAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGCGCCAGAACAGGATTAATACGGAAATAAGTCACGGGAAATTACTTCGTCCACCCTTTTTGTTTCAGGTAGTCGGCAGCCACTTTTTTGGCATCCAGTCCTTCCACAGCAATGCt                             <  2:1528374/150‑1 (MQ=255)
                                                                                                                        aGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGCGCCAGAACAGGATTAATACGGAAATAAGTCACGGGAAATTACTTCGTCCACCCTTTTTGTTTCAGGTAGTCGGCAGCCACTTTTTTGGCATCCAGTCCTTCCACAGCAATGCTg                            >  2:291870/1‑150 (MQ=255)
                                                                                                                         gcgcCGCTGCGATTGCCGTCAGCAACAGCAGCAGCGCCAGAACAGGATTAATACGGAAATAAGTCACGGGAAATTACTTCGTCCACCCTTTTTGTTTCAGGTAGTCGGCAGCCACTTTTTTGGCATCCAGTCCTTCCACAGCAATGCTgg                           <  1:1154780/150‑1 (MQ=255)
                                                                                                                          cgctgcTGCTGTTGCTGACGGCAATCGCAGCGGCGCCAGAACAGGATTAATACGGAAATAAGTCACGGGAAATTACTTCGTCCACCCTTTTTGTTTCAGGTAGTCGGCAGCCACTTTTTTGGCATCCAGTCCTTCCACAGCAATGCTGGc                          >  2:1009556/1‑150 (MQ=38)
                                                                                                                          cgctgcTGCTGTTGCTGACGGCAATCGCAGCGGCGCCAGAACAGGATTAATACGGAAATAAGTCACGGGAAATTACTTCGTCCACCCTTTTTGTTTCAGGTAGTCGGCAGCCACTTTTTTGGCAACCAGTCCTTCCACAGCAATGCTGGc                          >  1:706075/1‑150 (MQ=38)
                                                                                                                          cgccgcTGCGATTGCCGTCAGCAACAGCAGCAGCGCCAGAACAGGATTAATACGGAAATAAGTCACGGGAAATTACTTCGTCCACCCTTTTTGTTTCAGGTAGTCGGCAGCCACTTTTTTGGCATCCAGTCCTTCCACAGCAATGCTGGc                          >  1:1715461/1‑150 (MQ=255)
                                                                                                                          cgccgcTGCGATTGCCGTCAGCAACAGCAGCAGCGCCAGAACAGGATTAATACGGAAATAAGTCACGGGAAATTACTTCGTCCACCCTTTTTGTTTCAGGTAGTCGGCAGCCACTTTTTTGGCATCCAGTCCTTCCACAGCAATGCTGGc                          >  1:788719/1‑150 (MQ=255)
                                                                                                                           gccgcTGCGATTGCCGTCAGCAACAGCAGCAGCGCAAGAACAGGATTAATAAGGAAATAAGTCACGGGAAATTACTTCGTCCACCCTTTTTGTTTCAGGTAGTCGGCAGCCACTTTTTTGGCATACATTCATTCCACAGCAATGCTGGCa                         >  2:176049/1‑150 (MQ=255)
                                                                                                                             cgcTGCGATTGCCGTCAGCAACAGCAGCAGCGCCAGAACAGGATTAATACGGAAATAAGTCACGGGAAATTACTTCGTCCACCCTTTTTGTTTCAGGTAGTCGGCAGCCACTTTTTTGGCATCCAGTCCTTCCACAGCAATGCTGGCAtt                       >  2:475036/1‑150 (MQ=255)
                                                                                                                                tGCGATTGCCGTCAGCAACAGCAGCAGCGCCAGAACAGGATTAATACGGAAATAAGTCACGGGAAATTACTTCGTCCACCCTTTTTGTTTCAGGTAGTCGGCAGCCACTTTTTTGGCATCCAGTCCTTCCACAGCAATGCTGGCATTCAg                    <  1:1263221/150‑1 (MQ=255)
                                                                                                                                   gATTGCCGTCAGCAACAGCAGCAGCGCCAGAACAGGATTAATACGGTAATAAGTCACGGGAAATTACTTCGTCCACCCTTTTTGTTTCAGGTAGTCGGAAGCCACTTTATTGGCATCCAGTCCTTCCACAGCAATGCTGGCATTCAGTtg                 >  1:1526206/1‑150 (MQ=255)
                                                                                                                                    aTTGCCGTCAGCAACAGCAGCAGCGCCAGAACAGGATTAATACGGAAATAAGTCACGGGAAATTACTTCGTCCACCCTTTTTGTTTCAGGTAGTCGGCAGCCACTTTTTTGGCATCCAGTCCTTCCACAGCAATGCTGGCATTCAGTtgc                <  2:1256561/150‑1 (MQ=255)
                                                                                                                                      tGCCGTCAGCAACAGCAGCAGCGCCAGAACAGGATTAATACGGAAATAAGTCACGGGAAATTACTTCGTCCACCCTTTTTGTTTCAGGTAGTCGGCAGCCACTTTTTTGGCATCCAGTCCTTCCACAGCAATGCTGGCATTCAGTtgctg              <  1:1161152/150‑1 (MQ=255)
                                                                                                                                      tGCCGTCAGCAACAGCAGCAGCGCCAGAACAGGATTAATACGGAAATAAGTCACGGGAAATTACTTCGTCCACCCTTTTTGTTTCAGGTAGTCGGCAGCCACTTTTTTGGCATCCAGTCCTTCCACAGCAATGCTGGCATTCAGTtgctg              <  1:1008562/150‑1 (MQ=255)
                                                                                                                                      tGCCGTCAGCAACAGCAGCAGCGCCAGAACAGGATTAATACGGAAATAAGTCACGGGAAATTACTTCGTCCACCCTTTTTGTTTCAGGTAGTCGGCAGCCACTTTTTTGGCATCCAGTCCTTCCACAGCAATGCTGGCATTCAGTtgctg              <  1:24979/150‑1 (MQ=255)
                                                                                                                                       gCCGTCAGCAACAGCAGCAGCGCCAGAACAGGATTAATCCGGAAATAAGTCACGGGAAATTACTTCGTCCACCCTTTTTGTTTCAGGTAGTCGGCAGCCACTTTTTTGGCATCCAGTCCTTCCACAGCAATGCTGGCATTCAGTtgctgc             <  1:252243/150‑1 (MQ=255)
                                                                                                                                       gCCGTCAGCAACAGCAGCAGCGCCAGAACAGGATTAATACGGAAATAAGTCACGGGAAATTACTTCGTCCACCCTTTTTGTTTCAGGTAGTCGGCAGCCACTTTTTTGGCATCCAGTCCTTCCACAGCAATGCTGGCATTCAGTtgctga             <  1:176049/150‑2 (MQ=255)
                                                                                                                                       gCCGTCAGCAACAGCAGCAGCGCCAGAACAGGATTAATACGGAAATAAGTCACGGGAAATTACTTCGTCCACCCTTTTAGTTTCAGGTAGTCGGCAGCCACTTTTTTGGCATCCAGTCCTTCCACAGCAATGCTGGCATTCAGTtgctgc             >  1:1651804/1‑150 (MQ=255)
                                                                                                                                         cGTCAGCAACAGCAGCAGCGCCAGAACAGGATTAATACGGAAATAAGTCACGGGAAATTACTTCGTCCACCCTTTTTGTTTCAGGTAGTCGGCAGCCACTTTTTTGGCATCCAGTCCTTCCACAGCAATGCTGGCATTCAGATGCTGCaa           >  1:275316/1‑150 (MQ=255)
                                                                                                                                          gTCAGCAACAGCAGCAGCGCCAGAACAGGATTAATACGGAAATAAGTCACGGGAAATTACTTCGTCCACCCTTTTTGTTTCAGGTAGTCGGCAGCCACTTTTTTGGCATCCAGTCCTTCCACAGCAATGCTGGCATTCAGTTGCTGCAAt          >  2:599683/1‑150 (MQ=255)
                                                                                                                                           tCAGCAACAGCAGCAGCGCCAGAACAGGATTAATACGGAAATAAGTCACGGGAAATTACTTCGTCCACCCTTTTTGTTTCAGGTAGTCGGCAGCCACTTTTTTGGCATCCAGTCCTTCCACAGCAATGCTGGCATTCAGTTGCTGCAATg         <  2:214411/150‑1 (MQ=255)
                                                                                                                                           tCAGCAACAGCAGCAGCGCCAGAACAGGATTAATACGGAAATAAGTCACGGGAAATTACTTCGTCCACCCTTTTTGTTTCAGGTAGTCGGCAGCCACTTTTTTGGCATCCAGTCCTTCCACAGCAATGCTGGCATTCAGTTGCTGCAATg         <  2:1322719/150‑1 (MQ=255)
                                                                                                                                            cAGCAACAGCAGCAGCGCCAGAACAGGATTAATACGGAAATAAGTCACGGGAAATTACTTCGTCCACCCTTTTTGTTTCAGGTAGTCGGCAGCCACTTTTTTGGCATCCAGTCCTTCCACAGCAATGCTGGCATTCAGTTGCTGCAATGt        >  1:870727/1‑150 (MQ=255)
                                                                                                                                                aaCAGCAGCAGCGCCAGAACAGGATTAATACGGAAATAAGTCACGGGAAATTACTTCGTCCACCCTTTTTGTTTCAGGTAGTCGGCAGCCACTTTTTTGGCATCCAGTCCTTCCACAGCAATGCTGGCATTCAGTTGCTGCAATGttttt    >  1:94166/1‑150 (MQ=255)
                                                                                                                                                  cagcagcagCGCCAGAACAGGATTAATACGGAAATAAGTCACGGGAAATTATTTCGTCCACCCTTTTTGTTTCAGGTAGTCGGCAGCCACTTTTTTGGCATCCAGTCCTTCCACAGCAATGCTGGCATTCAGTTGCTGCAATGTTTTTGc  <  1:891419/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CAGGCCGTCAGCCAGGCGCAACCAACGCCCACCAGCATCCAGATCGTTTGCGGCCACAGCTGCCAGAGATGACGCCCCTCACCCGAAACTAAACGATTAGGCGCGTAACTGATAAACGGCAGCGCCGCTGCGATTGCCGTCAGCAACAGCAGCAGCGCCAGAACAGGATTAATACGGAAATAAGTCACGGGAAATTACTTCGTCCACCCTTTTTGTTTCAGGTAGTCGGCAGCCACTTTTTTGGCATCCAGTCCTTCCACAGCAATGCTGGCATTCAGTTGCTGCAATGTTTTTGC  >  NC_000913/2218370‑2218665

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: