Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,230,690 T→C 7.0% Y23H (TAT→CAT) ‡ yohJ → UPF0299 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,230,6900TC 7.0% 329.8 / 11.8 114Y23H (TAT→CAT) ‡yohJUPF0299 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (50/56);  new base C (5/3);  total (55/59)
Fisher's exact test for biased strand distribution p-value = 4.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 114 total aligned reads displayed.

CGCTGATTTCTTAATGTGATCGGTAGCACGTTTTACGAATTAATTGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCGGCAAAATGGGTCAATCCGGGGTGCTACGTACTGATTCGCTATA  >  NC_000913/2230545‑2230834
                                                                                                                                                 |                                                                                                                                                
cgcTGATTTCTTAATGTGATCGGTAGCACGTTTTACGAATTAATTGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGACTTATGCCTGCCTGTATGc                                                                                                                                              >  2:521201/1‑150 (MQ=255)
 gcTGATTTCTTAATGTGATCGGTAGCAAGTTTTACGAATTAATTGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCa                                                                                                                                             <  2:309275/150‑1 (MQ=255)
  cTGATTTCTTAATGTGATCGGTAGCACGTTTTACGAATTAATTGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAg                                                                                                                                            <  1:895409/150‑1 (MQ=255)
     agttCTTAATGTGATCGGTAGCACGTTTTACGAATTAATTGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTAGCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCa                                                                                                                                         <  1:257423/148‑1 (MQ=255)
      tttCTTAATGTGATCGGTAGCACGTTTTACGAATTTATTGGATGATGAATCCATCTCCTATGGGGTGTTGATTTTGTGTTAGCCACTGAACATTATCTGGGAATTTTTACGCGCTTTGGGGCTGATTCATGCCTGGCGGTATGCAgggct                                                                                                                                        >  2:699353/1‑147 (MQ=255)
          ttAATGTGATCGGTAGCACGTTTTACGAATTAATTGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGCCACTGAACATTATCTGGCAATATTTACAGGCTTTCGTCCTGATTTATGACTGCATGTATGCAGGCATTTTc                                                                                                                                    >  2:541128/1‑150 (MQ=255)
          ttAATGTGATCGGTAGCACGTTTTACGAATTAATTGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTc                                                                                                                                    >  2:337855/1‑150 (MQ=255)
           tAATGTGATCGGTAGCACGTTTTACGAATTAATTGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCa                                                                                                                                   <  1:85683/150‑1 (MQ=255)
             aTGTGATCGGTAGCACGTTTTACGAATTAATTGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCAtt                                                                                                                                 <  1:427959/150‑1 (MQ=255)
             aTGTGATCGGTAGCACGTTTTACGAATTAATTGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCATACAGGCAGGCAtaaatcag                                                                                                                                 <  2:30691/150‑8 (MQ=255)
               gtgATCGGTAGCACGTTTTACGAATTAATTGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGc                                                                                                                               >  1:1084695/1‑150 (MQ=255)
                   tCGGTAGCACGTTTTACGAATTAATTGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTcc                                                                                                                           <  2:1157276/150‑1 (MQ=255)
                   tCGGTAGCACGTTTTACGAATTAATTGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTcc                                                                                                                           <  2:474059/150‑1 (MQ=255)
                     ggTAGCACGTTTTACGAATTAATTGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCt                                                                                                                         >  2:139683/1‑150 (MQ=255)
                     ggTAGCACGTTTTACGAATTAATTGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCt                                                                                                                         >  2:1170604/1‑150 (MQ=255)
                      gTAGCACGTTTTACGAATTAATTGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGTCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTg                                                                                                                        >  2:324873/1‑150 (MQ=255)
                      gTAGCACGTTTTACGAATTAATTGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTg                                                                                                                        >  1:780880/1‑150 (MQ=255)
                      gTAGCACGTTTTACGAATTAATTGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTg                                                                                                                        <  1:180899/150‑1 (MQ=255)
                       tAGCACGTTTTACGAATTAATTGTATGATGAATCCCTCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGc                                                                                                                       <  2:626821/150‑1 (MQ=255)
                       tAGCACGTTTTACGAATTAATTGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGc                                                                                                                       <  2:996584/150‑1 (MQ=255)
                         gCACGTTTTACGAATTAATTGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTACTGATTTATGCCTGCATGTATGCAGGCATTTTCATTGCTTCCCTGCTa                                                                                                                     >  1:62028/1‑150 (MQ=255)
                          cACGTTTTACGAATTAATTGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTAc                                                                                                                    <  1:401497/150‑1 (MQ=255)
                            cGTTTTACGAATTAATTGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCg                                                                                                                  >  2:246528/1‑150 (MQ=255)
                            cGTTTTACGAATTAATTGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCATACAGGCAGGCAtaaatca                                                                                                                                  >  2:894422/1‑128 (MQ=255)
                             gTTTTACGAATTAATTGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCgg                                                                                                                 <  2:801943/150‑1 (MQ=255)
                              ttttACGAATTAATTGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGt                                                                                                                <  1:1419868/150‑1 (MQ=255)
                               tttACGAATTTATTGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTa                                                                                                               <  2:474486/150‑1 (MQ=255)
                                 tACGAATTAATTGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAAc                                                                                                             <  2:483027/150‑1 (MQ=255)
                                 tACGAATTAATTGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAAc                                                                                                             <  1:358934/150‑1 (MQ=255)
                                  aCGAATTAATTGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAAcc                                                                                                            >  2:718976/1‑150 (MQ=255)
                                  aCGAATTAATTGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAAcc                                                                                                            >  2:357442/1‑150 (MQ=255)
                                        taattGTATTATGAATACATCTCATCTGGTTTTTTGATTATGAGTAAGACACTTAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCg                                                                                                      <  2:997167/150‑1 (MQ=255)
                                        taattGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATTCAGGCATTTTCATTCCTTACCTGCTACCTGTAACCATTCCg                                                                                                      >  2:730499/1‑150 (MQ=255)
                                        taattGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCg                                                                                                      >  1:374761/1‑150 (MQ=255)
                                        taattGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCg                                                                                                      >  1:949276/1‑150 (MQ=255)
                                           ttGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGgc                                                                                                   <  2:780880/150‑1 (MQ=255)
                                            tGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACTCGCTTTAGTCCTGATTTATGCCTGCCTTTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGgca                                                                                                  >  1:88043/1‑150 (MQ=255)
                                                tgatgaATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGcat                                                                                              >  1:690634/1‑150 (MQ=255)
                                                     aaTCCATCTCATCTGGGGTGTGGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCg                                                                                         <  2:663610/150‑1 (MQ=255)
                                                      aTCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCgg                                                                                        <  1:337855/150‑1 (MQ=255)
                                                       tCCATCTCATCTGGGGTGTTGATTATGTGTAAGACCCTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCggg                                                                                       <  2:494863/150‑1 (MQ=255)
                                                         catctcatctGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGAt                                                                                     >  1:779904/1‑150 (MQ=255)
                                                           tctcatctGGGGTGTTGATTATGCGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATAGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGc                                                                                   >  2:1072456/1‑150 (MQ=255)
                                                                actGGGGTGATGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCATTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATc                                                                              <  1:280155/149‑1 (MQ=255)
                                                                 ctGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTTGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATcc                                                                             <  2:822611/150‑1 (MQ=255)
                                                                   ggggTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTg                                                                           >  1:990385/1‑150 (MQ=255)
                                                                     ggTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGtt                                                                         >  1:883245/1‑150 (MQ=255)
                                                                     ggTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGtt                                                                         >  1:856651/1‑150 (MQ=255)
                                                                      gtgtTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGttt                                                                        <  2:416235/150‑1 (MQ=255)
                                                                        gtTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGt                                                                      >  1:1515630/1‑150 (MQ=255)
                                                                               aTGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCctgctg                                                               >  2:234892/1‑150 (MQ=255)
                                                                                   ttAAGACACTGAACATTATCTTGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCt                                                           <  2:62028/149‑1 (MQ=255)
                                                                                      aGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGc                                                        <  2:1652005/150‑1 (MQ=255)
                                                                                       gACACTTAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCa                                                       <  2:949276/150‑1 (MQ=255)
                                                                                        acacTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGATGGCCTTGCaa                                                      >  1:303372/1‑150 (MQ=255)
                                                                                         cacTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCaaa                                                     >  1:1208619/1‑150 (MQ=255)
                                                                                         cacTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCATGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCAGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCaaa                                                     >  1:707869/1‑150 (MQ=255)
                                                                                             gAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAAttct                                                 >  2:685254/1‑150 (MQ=255)
                                                                                                  ttATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTCTGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCgg                                            <  1:240470/150‑1 (MQ=255)
                                                                                                  ttATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCgg                                            <  2:88043/150‑1 (MQ=255)
                                                                                                  ttATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCgg                                            <  2:990385/150‑1 (MQ=255)
                                                                                                  ttATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCgg                                            <  1:1039331/150‑1 (MQ=255)
                                                                                                  ttATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCgg                                            <  1:1588501/150‑1 (MQ=255)
                                                                                                  ttATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCgg                                            <  1:685254/150‑1 (MQ=255)
                                                                                                  ttATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTAGCCTTGCAAATTCTTCCgg                                            <  1:246528/150‑1 (MQ=255)
                                                                                                    aTCTGGCAATATTAACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCGGCa                                          <  2:201214/150‑1 (MQ=255)
                                                                                                       tGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCAATCCGGGCAGCATCATCGGGATGCTGATCCTGTTAGTCCTGCTGGCCTTGCAAATTCTTCCGGCaaaa                                       >  2:947945/1‑150 (MQ=255)
                                                                                                         gCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCAATCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCGGCAAAATg                                     <  1:139683/150‑1 (MQ=255)
                                                                                                          cAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCGGCAAAATgg                                    >  2:409577/1‑150 (MQ=255)
                                                                                                           aaTATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCGGCAAAATggg                                   >  1:1449775/1‑150 (MQ=255)
                                                                                                            atatTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCGGCAAAATGGGt                                  <  1:1222678/150‑1 (MQ=255)
                                                                                                            atatTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCAGGCAAAATgggg                                  >  2:1549255/1‑149 (MQ=255)
                                                                                                             tatTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCGGCAAAATGGGTc                                 <  2:1463502/150‑1 (MQ=255)
                                                                                                             tatTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCGGCAAAATGGGTc                                 <  2:1319242/150‑1 (MQ=255)
                                                                                                             aaattACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCGGCAAAATGGGTc                                 <  2:755898/147‑1 (MQ=255)
                                                                                                                   cgcgCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCGGCAAAATGGGTCAATCCg                           >  1:1030866/1‑150 (MQ=255)
                                                                                                                     cgcTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCGGCAAAATGGGTCAATCCggg                         <  2:1669352/150‑1 (MQ=255)
                                                                                                                      gcTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCGGCAAAATGGGTCAATCCgggg                        >  2:1329098/1‑150 (MQ=255)
                                                                                                                      gcTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTACCTGCTACCGGTAACCATTCCGGGCAGCATCATCTGTATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCGGCAAAATGGGTCAATCCgggg                        <  2:902578/150‑1 (MQ=255)
                                                                                                                        tttCGTCCTGATTTATGTCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCGTCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCGGCAAAATGGGTCAATCCGGGGTg                      <  1:324873/150‑1 (MQ=255)
                                                                                                                        tttCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCGGCAAAATGGGTCAATCCGGGGTg                      >  1:1206982/1‑150 (MQ=255)
                                                                                                                           cGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCGGCAAAATGGGTCAATCCGGGGTGCTa                   >  1:78712/1‑150 (MQ=255)
                                                                                                                           cGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCGGCAAAATGGGTCAATCCGGGGTGCTa                   >  1:1527573/1‑150 (MQ=255)
                                                                                                                            gTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCGGCAAAATGGGTCAATCCGGGGTGCTAc                  >  2:1428851/1‑150 (MQ=255)
                                                                                                                             tCCTGACTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCGGCAAAATGGGTCAATCCGGGGTGCTACg                 <  1:521201/150‑1 (MQ=255)
                                                                                                                              ccTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTTCCGGTAACCATTCCGGGCAGCATCATCGGGCTGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCGGCATAATGGGTCATTCCGGGGTGCTACGt                >  1:1380927/1‑150 (MQ=255)
                                                                                                                               cTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCGGCAAAATGGGTCAATCCGGGGTGCTACGTa               <  1:1683208/150‑1 (MQ=255)
                                                                                                                                atgaaaaTGCCTGCATACAGGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCGGCAAAATGGGTCAATCCGGGGTGCTACGTAc              >  1:1330737/7‑150 (MQ=255)
                                                                                                                                 tgaaaaTGCCTGCATACATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCGGCAAAATGGGTCAATCCGGGGTGCTACGTACt             >  2:1504211/6‑150 (MQ=255)
                                                                                                                                 gATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCTGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCGGCAAAATGGGTCAATCCGGGGTGCTACGTACt             >  1:110952/1‑150 (MQ=255)
                                                                                                                                 gATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAAGCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCGGCAAAATGGGTCAATCCGGGGTGCGACGTAcg             >  1:1055301/1‑149 (MQ=255)
                                                                                                                                     tATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCGGCAAAATgg                                    >  1:601463/1‑123 (MQ=255)
                                                                                                                                     tATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCGGCAAAATgg                                    <  2:601463/123‑1 (MQ=255)
                                                                                                                                     tATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCGGCAAAATGGGTCAATCCGGGGTGCTACGTACTGAtt         >  2:534607/1‑150 (MQ=255)
                                                                                                                                     tATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCGGCAAAATGGGTCAATCCGGGGTGCTACGTACTGAtt         <  2:1449775/150‑1 (MQ=255)
                                                                                                                                      aTGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCGGCAAAATGGGTCAATCCGGGGTGCTACGTACTGATTc        <  2:1208619/150‑1 (MQ=255)
                                                                                                                                      aTGCCTGCATACAGGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCGGCAAAATGGGTCAATCCGGGGTGCTACGTACt             >  2:1552635/1‑145 (MQ=255)
                                                                                                                                       tgcctgccTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCGGCAAAATGGGTCAATCCGGGGTGCTACGTACTGATTCg       >  2:1381643/1‑150 (MQ=255)
                                                                                                                                         cctgcctgTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCGGCAAAATGGGTCAATCCGGGGTGCTACGTACTGATTCGCt     >  2:797465/1‑150 (MQ=255)
                                                                                                                                            gcctgTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCGGCAAAATGGGGCAATCCGGGGTGCTACGTACTGATTCGCtata  <  1:718976/150‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
CGCTGATTTCTTAATGTGATCGGTAGCACGTTTTACGAATTAATTGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCGGCAAAATGGGTCAATCCGGGGTGCTACGTACTGATTCGCTATA  >  NC_000913/2230545‑2230834

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: