Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,230,692 T→G 6.0% Y23* (TAT→TAG) ‡ yohJ → UPF0299 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,230,6920TG 6.0% 346.6 / 9.8 116Y23* (TAT→TAG) ‡yohJUPF0299 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (52/57);  new base G (4/3);  total (56/60)
Fisher's exact test for biased strand distribution p-value = 7.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.05e-01

Only 100 of 117 total aligned reads displayed.

CGCTGATTTCTTAATGTGATCGGTAGCACGTTTTACGAATTAATTGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCGGCAAAATGGGTCAATCCGGGGTGCTACGTACTGATTCGCTATATGGCGCT  >  NC_000913/2230545‑2230841
                                                                                                                                                   |                                                                                                                                                     
 gcTGATTTCTTAATGTGATCGGTAGCAAGTTTTACGAATTAATTGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCa                                                                                                                                                    <  2:309275/150‑1 (MQ=255)
  cTGATTTCTTAATGTGATCGGTAGCACGTTTTACGAATTAATTGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAg                                                                                                                                                   <  1:895409/150‑1 (MQ=255)
     agttCTTAATGTGATCGGTAGCACGTTTTACGAATTAATTGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTAGCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCa                                                                                                                                                <  1:257423/148‑1 (MQ=255)
      tttCTTAATGTGATCGGTAGCACGTTTTACGAATTTATTGGATGATGAATCCATCTCCTATGGGGTGTTGATTTTGTGTTAGCCACTGAACATTATCTGGGAATTTTTACGCGCTTTGGGGCTGATTCATGCCTGGCGGTATGCAgggct                                                                                                                                               >  2:699353/1‑147 (MQ=255)
          ttAATGTGATCGGTAGCACGTTTTACGAATTAATTGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGCCACTGAACATTATCTGGCAATATTTACAGGCTTTCGTCCTGATTTATGACTGCATGTATGCAGGCATTTTc                                                                                                                                           >  2:541128/1‑150 (MQ=255)
          ttAATGTGATCGGTAGCACGTTTTACGAATTAATTGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTc                                                                                                                                           >  2:337855/1‑150 (MQ=255)
           tAATGTGATCGGTAGCACGTTTTACGAATTAATTGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCa                                                                                                                                          <  1:85683/150‑1 (MQ=255)
             aTGTGATCGGTAGCACGTTTTACGAATTAATTGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCAtt                                                                                                                                        <  1:427959/150‑1 (MQ=255)
             aTGTGATCGGTAGCACGTTTTACGAATTAATTGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCATACAGGCAGGCAtaaatcag                                                                                                                                        <  2:30691/150‑8 (MQ=255)
               gtgATCGGTAGCACGTTTTACGAATTAATTGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGc                                                                                                                                      >  1:1084695/1‑150 (MQ=255)
                   tCGGTAGCACGTTTTACGAATTAATTGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTcc                                                                                                                                  <  2:474059/150‑1 (MQ=255)
                   tCGGTAGCACGTTTTACGAATTAATTGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTcc                                                                                                                                  <  2:1157276/150‑1 (MQ=255)
                     ggTAGCACGTTTTACGAATTAATTGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCt                                                                                                                                >  2:139683/1‑150 (MQ=255)
                      gTAGCACGTTTTACGAATTAATTGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGTCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTg                                                                                                                               >  2:324873/1‑150 (MQ=255)
                      gTAGCACGTTTTACGAATTAATTGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTg                                                                                                                               >  1:780880/1‑150 (MQ=255)
                       tAGCACGTTTTACGAATTAATTGTATGATGAATCCCTCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGc                                                                                                                              <  2:626821/150‑1 (MQ=255)
                       tAGCACGTTTTACGAATTAATTGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGc                                                                                                                              <  2:996584/150‑1 (MQ=255)
                         gCACGTTTTACGAATTAATTGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTACTGATTTATGCCTGCATGTATGCAGGCATTTTCATTGCTTCCCTGCTa                                                                                                                            >  1:62028/1‑150 (MQ=255)
                          cACGTTTTACGAATTAATTGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTAc                                                                                                                           <  1:401497/150‑1 (MQ=255)
                           aCGTTTTACGAATTAATTGTATGATGAATCCATCGCATCTGGGGTGTTGTTTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCTGGCATTTTCATTGCTTCCCTGCTAcc                                                                                                                          <  1:1322761/150‑1 (MQ=255)
                            cGTTTTACGAATTAATTGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCATACAGGCAGGCAtaaatca                                                                                                                                         <  1:894422/134‑7 (MQ=255)
                            cGTTTTACGAATTAATTGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCATACAGGCAGGCAtaaatca                                                                                                                                         >  2:894422/1‑128 (MQ=255)
                             gTTTTACGAATTAATTGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCgg                                                                                                                        <  2:801943/150‑1 (MQ=255)
                              ttttACGAATTAATTGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGt                                                                                                                       <  1:1419868/150‑1 (MQ=255)
                               tttACGAATTTATTGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTa                                                                                                                      <  2:474486/150‑1 (MQ=255)
                                 tACGAATTAATTGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAAc                                                                                                                    <  2:483027/150‑1 (MQ=255)
                                 tACGAATTAATTGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAAc                                                                                                                    <  1:358934/150‑1 (MQ=255)
                                  aCGAATTAATTGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAAcc                                                                                                                   >  2:718976/1‑150 (MQ=255)
                                  aCGAATTAATTGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAAcc                                                                                                                   >  2:357442/1‑150 (MQ=255)
                                   cGAATTAATTGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCa                                                                                                                  >  2:728647/1‑150 (MQ=255)
                                        taattGTATTATGAATACATCTCATCTGGTTTTTTGATTATGAGTAAGACACTTAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCg                                                                                                             <  2:997167/150‑1 (MQ=255)
                                        taattGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATTCAGGCATTTTCATTCCTTACCTGCTACCTGTAACCATTCCg                                                                                                             >  2:730499/1‑150 (MQ=255)
                                        taattGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCg                                                                                                             >  1:374761/1‑150 (MQ=255)
                                        taattGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCg                                                                                                             >  1:949276/1‑150 (MQ=255)
                                            tGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACTCGCTTTAGTCCTGATTTATGCCTGCCTTTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGgca                                                                                                         >  1:88043/1‑150 (MQ=255)
                                             gTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATTCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGgcac                                                                                                        >  1:287083/1‑149 (MQ=255)
                                                tgatgaATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGcat                                                                                                     >  1:690634/1‑150 (MQ=255)
                                                     aaTCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCg                                                                                                >  2:450155/1‑150 (MQ=255)
                                                      aTCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCgg                                                                                               <  1:337855/150‑1 (MQ=255)
                                                       tCCATCTCATCTGGGGTGTTGATTATGTGTAAGACCCTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCggg                                                                                              <  2:494863/150‑1 (MQ=255)
                                                         catctcatctGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGAt                                                                                            >  1:779904/1‑150 (MQ=255)
                                                           tctcatctGGGGTGTTGATTATGCGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATAGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGc                                                                                          >  2:1072456/1‑150 (MQ=255)
                                                                actGGGGTGATGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCATTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATc                                                                                     <  1:280155/149‑1 (MQ=255)
                                                                 ctGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTTGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATcc                                                                                    <  2:822611/150‑1 (MQ=255)
                                                                   ggggTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTg                                                                                  >  1:990385/1‑150 (MQ=255)
                                                                     ggTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGtt                                                                                >  2:1222678/1‑150 (MQ=255)
                                                                     ggTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGtt                                                                                >  1:856651/1‑150 (MQ=255)
                                                                      gtgtTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGttt                                                                               <  2:416235/150‑1 (MQ=255)
                                                                        gtTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGt                                                                             >  1:1515630/1‑150 (MQ=255)
                                                                               aTGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCctgctg                                                                      >  2:234892/1‑150 (MQ=255)
                                                                                   ttAAGACACTGAACATTATCTTGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCt                                                                  <  2:62028/149‑1 (MQ=255)
                                                                                      aGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGc                                                               <  2:1652005/150‑1 (MQ=255)
                                                                                       gACACTTAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCa                                                              <  2:949276/150‑1 (MQ=255)
                                                                                        acacTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGATGGCCTTGCaa                                                             >  1:303372/1‑150 (MQ=255)
                                                                                         cacTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCATGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCAGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCaaa                                                            >  1:707869/1‑150 (MQ=255)
                                                                                          acTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAAt                                                           <  2:883245/150‑1 (MQ=255)
                                                                                             gAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAAttct                                                        >  2:685254/1‑150 (MQ=255)
                                                                                                  ttATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTCTGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCgg                                                   <  1:240470/150‑1 (MQ=255)
                                                                                                  ttATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCgg                                                   <  1:1039331/150‑1 (MQ=255)
                                                                                                  ttATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCgg                                                   <  2:88043/150‑1 (MQ=255)
                                                                                                  ttATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCgg                                                   <  2:990385/150‑1 (MQ=255)
                                                                                                  ttATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCgg                                                   <  1:1588501/150‑1 (MQ=255)
                                                                                                  ttATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCgg                                                   <  1:685254/150‑1 (MQ=255)
                                                                                                  ttATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTAGCCTTGCAAATTCTTCCgg                                                   <  1:246528/150‑1 (MQ=255)
                                                                                                    aTCTGGCAATATTAACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCGGCa                                                 <  2:201214/150‑1 (MQ=255)
                                                                                                       tGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCAATCCGGGCAGCATCATCGGGATGCTGATCCTGTTAGTCCTGCTGGCCTTGCAAATTCTTCCGGCaaaa                                              >  2:947945/1‑150 (MQ=255)
                                                                                                         gCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCAATCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCGGCAAAATg                                            <  1:139683/150‑1 (MQ=255)
                                                                                                            atatTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCGGCAAAATGGGt                                         <  1:1222678/150‑1 (MQ=255)
                                                                                                            atatTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCAGGCAAAATgggg                                         >  2:1549255/1‑149 (MQ=255)
                                                                                                             tatTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCGGCAAAATGGGTc                                        <  2:1463502/150‑1 (MQ=255)
                                                                                                             tatTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCGGCAAAATGGGTc                                        <  2:1319242/150‑1 (MQ=255)
                                                                                                             aaattACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCGGCAAAATGGGTc                                        <  2:755898/147‑1 (MQ=255)
                                                                                                                   cgcgCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCGGCAAAATGGGTCAATCCg                                  >  1:1030866/1‑150 (MQ=255)
                                                                                                                     cgcTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCGGCAAAATGGGTCAATCCggg                                <  2:1669352/150‑1 (MQ=255)
                                                                                                                      gcTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCGGCAAAATGGGTCAATCCgggg                               >  2:1329098/1‑150 (MQ=255)
                                                                                                                        tttCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCGGCAAAATGGGTCAATCCGGGGTg                             >  1:1206982/1‑150 (MQ=255)
                                                                                                                           cGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCGGCAAAATGGGTCAATCCGGGGTGCTa                          >  1:78712/1‑150 (MQ=255)
                                                                                                                           cGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCGGCAAAATGGGTCAATCCGGGGTGCTa                          >  1:1527573/1‑150 (MQ=255)
                                                                                                                            gTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCGGCAAAATGGGTCAATCCGGGGTGCTAc                         >  2:1428851/1‑150 (MQ=255)
                                                                                                                              ccTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTTCCGGTAACCATTCCGGGCAGCATCATCGGGCTGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCGGCATAATGGGTCATTCCGGGGTGCTACGt                       >  1:1380927/1‑150 (MQ=255)
                                                                                                                               cTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCGGCAAAATGGGTCAATCCGGGGTGCTACGTa                      <  1:1683208/150‑1 (MQ=255)
                                                                                                                                atgaaaaTGCCTGCATACAGGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCGGCAAAATGGGTCAATCCGGGGTGCTACGTAc                     >  1:1330737/7‑150 (MQ=255)
                                                                                                                                 tgaaaaTGCCTGCATACATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCGGCAAAATGGGTCAATCCGGGGTGCTACGTACt                    >  2:1504211/6‑150 (MQ=255)
                                                                                                                                 gATTTATTCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCGGCAAAATGGGTCAATCCGGGGTGCTACGTACt                    <  2:287083/150‑1 (MQ=255)
                                                                                                                                 gATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCTGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCGGCAAAATGGGTCAATCCGGGGTGCTACGTACt                    >  1:110952/1‑150 (MQ=255)
                                                                                                                                 gATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAAGCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCGGCAAAATGGGTCAATCCGGGGTGCGACGTAcg                    >  1:1055301/1‑149 (MQ=255)
                                                                                                                                   aaaaTGCCTGCATACAGGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCGGCAAAATGGGTCAATCCGGGGTGCTACGTACTGa                  >  2:561306/4‑150 (MQ=255)
                                                                                                                                     tATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCGGCAAAATgg                                           >  1:601463/1‑123 (MQ=255)
                                                                                                                                     tATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCGGCAAAATgg                                           <  2:601463/123‑1 (MQ=255)
                                                                                                                                     tATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCGGCAAAATGGGTCAATCCGGGGTGCTACGTACTGAtt                <  2:1449775/150‑1 (MQ=255)
                                                                                                                                     tATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCGGCAAAATGGGTCAATCCGGGGTGCTACGTACTGAtt                >  2:534607/1‑150 (MQ=255)
                                                                                                                                      aTGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCGGCAAAATGGGTCAATCCGGGGTGCTACGTACTGATTc               <  1:450155/150‑1 (MQ=255)
                                                                                                                                      aTGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCGGCAAAATGGGTCAATCCGGGGTGCTACGTACTGATTc               <  2:1208619/150‑1 (MQ=255)
                                                                                                                                      aTGCCTGCATACAGGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCGGCAAAATGGGTCAATCCGGGGTGCTACGTACt                    >  2:1552635/1‑145 (MQ=255)
                                                                                                                                      aTGCCTGCATACAGGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCGGCAAAATGGGTCAATCCGGGGTGCTACGTACt                    <  1:1552635/145‑1 (MQ=255)
                                                                                                                                         cctgcctgTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCGGCAAAATGGGTCAATCCGGGGTGCTACGTACTGATTCGCt            >  2:797465/1‑150 (MQ=255)
                                                                                                                                            gcctgTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCGGCAAAATGGGGCAATCCGGGGTGCTACGTACTGATTCGCtata         <  1:718976/150‑1 (MQ=255)
                                                                                                                                                  aTGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCGGCAAAATGGGTCAATCCGGGGTGCTACGTACTGATTCGCTATATGgcgc   <  2:1515630/150‑1 (MQ=255)
                                                                                                                                                  aTGCAGGCATTTTCATTGCTTCCCGGCTACCGGTAACCATTCCGGGCAGCATCATCGCGATGCTGATCCTGTTTGTGCTGCTGGCCGTGCAAATTCTTCGGGCAAAATGGGTCAATCGGGGGGGCTACGGACTGATTCGGTTTATGgcg  >  2:123458/1‑149 (MQ=255)
                                                                                                                                                   tGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCGGCAAAATGGGTCAATCCGGGGTGCTACGTACTGATTCGCTATATGGCGCt  >  1:1694748/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
CGCTGATTTCTTAATGTGATCGGTAGCACGTTTTACGAATTAATTGTATGATGAATCCATCTCATCTGGGGTGTTGATTATGAGTAAGACACTGAACATTATCTGGCAATATTTACGCGCTTTCGTCCTGATTTATGCCTGCCTGTATGCAGGCATTTTCATTGCTTCCCTGCTACCGGTAACCATTCCGGGCAGCATCATCGGGATGCTGATCCTGTTTGTCCTGCTGGCCTTGCAAATTCTTCCGGCAAAATGGGTCAATCCGGGGTGCTACGTACTGATTCGCTATATGGCGCT  >  NC_000913/2230545‑2230841

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: