Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,259,553 A→T 5.6% intergenic (‑257/+166) psuK ← / ← fruA pseudouridine kinase/fused fructose‑specific PTS enzymes: IIBcomponent/IIC components

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,259,5530AT 5.6% 387.9 / 6.8 131intergenic (‑257/+166)psuK/fruApseudouridine kinase/fused fructose‑specific PTS enzymes: IIBcomponent/IIC components
Reads supporting (aligned to +/- strand):  ref base A (60/62);  new base T (3/5);  total (64/67)
Fisher's exact test for biased strand distribution p-value = 7.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 131 total aligned reads displayed.

TGTTTTACGTAAAGTAAATTACCGCCAGAGAGGCAAAATGGGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCT  >  NC_000913/2259407‑2259698
                                                                                                                                                  |                                                                                                                                                 
   tttACGTAAAGTAAATTACCGCCAGAGAGGCAAAATGGGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGc                                                                                                                                             >  1:1719536/1‑150 (MQ=255)
   tttACGTAAAGTAAATTACCGCCAGAGAGGCAAAATGGGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGc                                                                                                                                             <  1:1251058/150‑1 (MQ=255)
   tttACGTAAAGTAAATTACCGCCAGACAGGCAAAATGGGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGc                                                                                                                                             <  1:1588329/150‑1 (MQ=255)
      aCGTAAAGTAATTTACTGCCAGAGAGGCAAACTGGGCAGATTTCAGCAGGCTCACATAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGTGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTTCCCt                                                                                                                                          <  2:148324/150‑1 (MQ=255)
      aCGTAAAGTAAATTACCGCCAGAGAGGCAAAATGGGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGATTATTTTCTGCCCt                                                                                                                                          <  1:1194971/150‑1 (MQ=255)
      aCGTAAAGTAAATTACCGCCAGAGAGGCAAAATGGGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCt                                                                                                                                          >  1:1006568/1‑150 (MQ=255)
       cGTAAAGTAAATTACCGCCAGAGAGGCAAAATGGGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCtt                                                                                                                                         <  2:652782/150‑1 (MQ=255)
        gtaaagtaaaTTACCGCCAGAGAGGCAAAATGGGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGATTATTTTCTGCCCttt                                                                                                                                        <  1:777940/150‑2 (MQ=255)
          aaagtaaaTTACCGCCAGAGAGGCAAAATGGGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAg                                                                                                                                      <  2:1181115/150‑1 (MQ=255)
           aagtaaaTTACCGCCAGAGAGGCAAAATGGGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGATTATTTTCTGCCCtttttt                                                                                                                                     <  2:183978/150‑5 (MQ=255)
           aagtaaaTTACCGCCAGAGAGGCAAAATGGGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAgg                                                                                                                                     >  1:1480742/1‑150 (MQ=255)
            agtaaaTTACCGCCAGAGAGGCAAAATGGGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGa                                                                                                                                    <  1:1014634/150‑1 (MQ=255)
             gtaaaTTACCGCCAGAGAGGCAAAATGGGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGATTATTTTCTGCCCttttttat                                                                                                                                   <  2:1681078/150‑7 (MQ=255)
                     ccGCCAGAGAGGCAAAATGGGTAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTg                                                                                                                           >  2:971518/1‑150 (MQ=255)
                     ccGCCAGAGAGGCAAAATGGGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTg                                                                                                                           >  2:581244/1‑150 (MQ=255)
                     ccGCCAGAGAGGCAAAATGGGCAGATTTGAGAAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGATGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTg                                                                                                                           <  1:1545597/150‑1 (MQ=255)
                       gCCAGAGAGGCAAAATGGGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAg                                                                                                                         >  1:201604/1‑150 (MQ=255)
                       gCCAGAGAGGCAAAATGGGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAg                                                                                                                         >  1:1069176/1‑150 (MQ=255)
                        ccAGATAGTCAAAATGGGCAGATTTGAGCATGCTCACAAAATGCGATTAAATGCATGAATTACAAAAGCGAGGAATTTGTGACTGGATGCACAGAACATAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAgt                                                                                                                        <  1:1345367/150‑1 (MQ=255)
                           gagagGCAAAATGGGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAACGGCAGAAAATAATCTGCCCTTGAGGAATTAACCTGAGTGtt                                                                                                                     >  1:835433/1‑150 (MQ=255)
                             gagGCAAAATGGGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTtg                                                                                                                   <  1:1009551/150‑1 (MQ=255)
                             gagGCAAAATGGGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTtg                                                                                                                   <  1:955436/150‑1 (MQ=255)
                               gtcAATATGGTCATATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAATCGAGGAATTTGTGACTGGATGCACAGAACAGAACATTAAAAAAGTGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTtgat                                                                                                                 <  1:917741/148‑1 (MQ=255)
                               ggCAAATTGGGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAATTAACCTGAGTGTTtgat                                                                                                                 <  2:1424345/150‑1 (MQ=255)
                               ggCAAAATGGGCAGATTTGATCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTtgat                                                                                                                 <  2:853833/150‑1 (MQ=255)
                               ggCAAAATGGGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTtgat                                                                                                                 >  2:1636924/1‑150 (MQ=255)
                                gCAAAATGGGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGATGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTtgatg                                                                                                                <  1:734908/150‑1 (MQ=255)
                                 cAAAATGGGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATTATCTGCCCTTGAGGAAATAACCTGAGTGTTtgatga                                                                                                               >  2:112661/1‑150 (MQ=255)
                                 cAAAATGGGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTtgatga                                                                                                               <  1:1608634/150‑1 (MQ=255)
                                  aaaaTGGGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGaa                                                                                                              >  2:680012/1‑150 (MQ=255)
                                        gtcAGATTTGAGCAGGCTCACAAAACGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTg                                                                                                        <  2:293435/148‑1 (MQ=255)
                                        ggCAGATTTGAGCAGGCTCACAAACTGCGCTTACATGCCTGAATTACAAAAGCGAGGAATTTGTGCCGGGATGCCCAGAACAGAACAATAAAAACTGGCAGAAAATAATCTCCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGgttt                                                                                                        >  1:57354/1‑149 (MQ=255)
                                        ggCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATACTCTGCCCTTGAGTAAATAAACTGAGTGTTTGATGAACGTTTg                                                                                                        >  1:1223809/1‑150 (MQ=255)
                                        ggCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGtttt                                                                                                        >  1:1390807/1‑149 (MQ=255)
                                        ggCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTg                                                                                                        >  1:1679632/1‑150 (MQ=255)
                                         gCATATTTGAGCAGGCTCACAAAATGCGATTAAATGACTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGa                                                                                                       <  1:1038066/150‑1 (MQ=255)
                                           aGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAg                                                                                                     <  2:1158301/150‑1 (MQ=255)
                                            gATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGTGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGt                                                                                                    >  1:1380586/1‑150 (MQ=255)
                                               ttGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTc                                                                                                 <  2:1679632/150‑1 (MQ=255)
                                               ttGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTc                                                                                                 <  1:680012/150‑1 (MQ=255)
                                                  aGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTAc                                                                                              <  2:201604/150‑1 (MQ=255)
                                                        cTCACAAAATGCGATTACATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAg                                                                                        <  1:1662839/150‑1 (MQ=255)
                                                          cacaAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGAt                                                                                      >  2:240028/1‑150 (MQ=255)
                                                          cacaAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGAt                                                                                      <  2:1034213/150‑1 (MQ=255)
                                                            caAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCGTGAGGAAATAACCTGAGTGTTTGTTGAACGTTTGAAGTTTCTACTGAAAGATaa                                                                                    <  1:913386/150‑1 (MQ=255)
                                                             aaaaTGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATaaa                                                                                   <  1:70482/150‑1 (MQ=255)
                                                               aaTGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAAc                                                                                 <  1:1636924/150‑1 (MQ=255)
                                                                  gCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCAt                                                                              >  1:381787/1‑150 (MQ=255)
                                                                        aaaTGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAAt                                                                        <  1:1245023/150‑1 (MQ=255)
                                                                        aaaTGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAAt                                                                        >  2:1259854/1‑150 (MQ=255)
                                                                           tGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCa                                                                     <  2:884762/150‑1 (MQ=255)
                                                                           tGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCa                                                                     <  2:1604414/150‑1 (MQ=255)
                                                                             ccTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATa                                                                   >  2:68563/1‑150 (MQ=255)
                                                                              cTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATaa                                                                  >  2:493330/1‑150 (MQ=255)
                                                                                  aTTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAAtt                                                              <  2:1069176/150‑1 (MQ=255)
                                                                                   ttACAAAAGCGAGGAATTTGTGACGGGTTGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTa                                                             >  2:1345527/1‑150 (MQ=255)
                                                                                     aCAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGACCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAAACATATAAATACATAAAATTACa                                                           >  2:680346/1‑150 (MQ=255)
                                                                                      cAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACAt                                                          <  2:1046423/150‑1 (MQ=255)
                                                                                       aaaaGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACAtt                                                         >  1:201077/1‑150 (MQ=255)
                                                                                        aaaGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTc                                                        >  1:869514/1‑150 (MQ=255)
                                                                                        aaaGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTc                                                        >  2:648243/1‑150 (MQ=255)
                                                                                         aaGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAATAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTca                                                       <  1:168492/150‑1 (MQ=255)
                                                                                           gCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTcaca                                                     >  2:1220082/1‑150 (MQ=255)
                                                                                           gCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTcaca                                                     <  1:1456459/150‑1 (MQ=255)
                                                                                              aGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAAc                                                  <  2:818476/150‑1 (MQ=255)
                                                                                               ggAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAAcc                                                 <  2:1220829/150‑1 (MQ=255)
                                                                                               ggAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAAcc                                                 >  2:1176597/1‑150 (MQ=255)
                                                                                                   tttGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTc                                             <  2:1719536/150‑1 (MQ=255)
                                                                                                      gtgACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCAtt                                          >  2:666206/1‑150 (MQ=255)
                                                                                                       tgACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATtc                                         <  2:1342308/150‑1 (MQ=255)
                                                                                                        gACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCa                                                                     <  2:612713/121‑1 (MQ=255)
                                                                                                        gACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCa                                                                     >  1:612713/1‑121 (MQ=255)
                                                                                                         aCGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATtctc                                       >  2:1567042/1‑150 (MQ=255)
                                                                                                            ggATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCttt                                    >  2:622265/1‑150 (MQ=255)
                                                                                                             gATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTg                                   <  2:201077/150‑1 (MQ=255)
                                                                                                              aTGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTgc                                  <  1:43541/150‑1 (MQ=255)
                                                                                                               tGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTgcg                                 >  2:299707/1‑150 (MQ=255)
                                                                                                                gCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATACCCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTgcgc                                >  1:83066/1‑150 (MQ=255)
                                                                                                                 cacaGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTg                                   <  1:73428/146‑1 (MQ=255)
                                                                                                                 cacaGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCt                               >  1:1267346/1‑150 (MQ=255)
                                                                                                                 cacaGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCt                               <  1:428359/150‑1 (MQ=255)
                                                                                                                 cacaGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCt                               >  1:217149/1‑150 (MQ=255)
                                                                                                                 cacaGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCt                               >  1:1216984/1‑150 (MQ=255)
                                                                                                                    agaacagatcAATAAAAAATGGCAGAAAATAATCTGCCCTTTAGGAAATAACCTGAGTGTTTTATGAACGTTTGAAGTTTCTAGTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCtcat                            <  1:680346/150‑1 (MQ=255)
                                                                                                                     gaacagaacaATAAAAAAGGGCAGAAAATATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCtcatca                          >  2:948550/1‑150 (MQ=255)
                                                                                                                        cagaacaATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCaaa                        >  1:595349/1‑150 (MQ=255)
                                                                                                                          gaacaATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATg                      <  1:1509425/150‑1 (MQ=255)
                                                                                                                           aacaATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGt                     <  1:971518/150‑1 (MQ=255)
                                                                                                                            acaATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGtt                    >  2:1611950/1‑150 (MQ=255)
                                                                                                                             caATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAAACTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTa                   <  2:835433/150‑1 (MQ=255)
                                                                                                                               aTAAAAAAGTTCAGAAAATAATCTGCCCTTGATGAAATAACCTTAGTTTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTaca                 <  1:932510/150‑1 (MQ=255)
                                                                                                                                 aaaaaaGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTacagg               <  1:1567042/150‑1 (MQ=255)
                                                                                                                                  aaaaaGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAAAGTTTGAAGTTTCTAATGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGTTCATTATCTTTGCGCTCATCAAATGTTacagga              >  1:895643/1‑150 (MQ=255)
                                                                                                                                    caaGGGCAGATTATTTTCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTacaggaca            >  1:309802/2‑150 (MQ=255)
                                                                                                                                     aaGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTacaggacag           >  2:172300/1‑150 (MQ=255)
                                                                                                                                     aaGGGCAGAAAATAATCTGCACTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTacaggacag           <  1:581244/150‑1 (MQ=255)
                                                                                                                                        ggCAGAAAATAATCTGCCCTTGAGGAAATAACATGACTGAATGATGAACGTTAGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATATAATTACATTCACAAAACGGTCATTCTCTTTGCGCTCATCAAAAGTTACATAACAGGaa        >  1:302963/1‑150 (MQ=255)
                                                                                                                                            gAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAAttt    >  1:474788/1‑150 (MQ=255)
                                                                                                                                            gAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAAttt    >  1:1296328/1‑150 (MQ=255)
                                                                                                                                              aaaTAATCTGCCCTTGAGGTAATAACCTGAGTGTTTGATGAACGTTTGTAGTTTCTACTGAAAGATAAAAGCATTTATATTCATAAAATTTCATTCACAAACCGGTCTTTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCt  <  2:302963/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
TGTTTTACGTAAAGTAAATTACCGCCAGAGAGGCAAAATGGGCAGATTTGAGCAGGCTCACAAAATGCGATTAAATGCCTGAATTACAAAAGCGAGGAATTTGTGACGGGATGCACAGAACAGAACAATAAAAAAGGGCAGAAAATAATCTGCCCTTGAGGAAATAACCTGAGTGTTTGATGAACGTTTGAAGTTTCTACTGAAAGATAAAACCATTTAAATTCATAAAATTACATTCACAAACCGGTCATTCTCTTTGCGCTCATCAAATGTTACAGGACAGGAAATTTCT  >  NC_000913/2259407‑2259698

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: