Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,267,739	C→G	8.1%	intergenic (+28/‑90)	yeiQ → / → yeiR	putative NAD‑dependent D‑mannonate oxidoreductase/Zn‑stimulated GTPase involved in zinc homeostasis; mutants are cadmium and EDTA sensitive; Zn(2+) binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,267,739	0	C	G	8.1%	282.3 / 12.6	86	intergenic (+28/‑90)	yeiQ/yeiR	putative NAD‑dependent D‑mannonate oxidoreductase/Zn‑stimulated GTPase involved in zinc homeostasis; mutants are cadmium and EDTA sensitive; Zn(2+) binding protein
Reads supporting (aligned to +/- strand):  ref base C (36/43);  new base G (5/2);  total (41/45)
Fisher's exact test for biased strand distribution p-value = 2.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01

TGAAGTTTTCGGTGATGATCTGCCAGATAACCCGCATTTTGTGCAGGCCATCGAACAAGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCT  >  NC_000913/2267591‑2267887                                                                                                                                                     |                                                                                                                                                     ggAAGTTTTCGGTGATGATCTGCCAGATAACCCGCATTTTGTGCAGGCCATCGAACATGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCt                                                                                                                                                     <  1:1136254/149‑1 (MQ=255)     gTTTTCGGTGATGATCTGCCAGATAACCCGCATTTTGTGCAGGCCATCGAACAAGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCAcc                                                                                                                                                 <  1:366042/150‑1 (MQ=255)     gTTTTCGGTGATGATCTGCCAGATAACCCGCATTTTGTGCAGGCCATCGAACAAGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCAcc                                                                                                                                                 >  2:1589445/1‑150 (MQ=255)     gTTTTCGGTGATGATCTGCCAGATAACCCGCATTTTGTGCAGGCCATCGAACAAGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCAcc                                                                                                                                                 >  1:519039/1‑150 (MQ=255)       tttCGGTGATGATCTGCCAGATAACCCGCATTTTGTGCAGGCCATCGAACAAGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCtt                                                                                                                                               <  2:705488/150‑1 (MQ=255)           ggTGATGATCTGCCAGATAACCCGCATTTTGTGCAGGTCATCGAACAAGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTc                                                                                                                                           <  1:490477/150‑1 (MQ=255)            gTGATGATCTGCCAGATAACCCGCATTTTGTGCAGGCCATCGAACAAGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTcc                                                                                                                                          <  2:519039/150‑1 (MQ=255)                tgatCTGCCAGATAACCCGCATTTTGTGCAGGCCATCGAACAAGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGctc                                                                                                                                      <  1:915145/150‑1 (MQ=255)                tgatCTGCCAGATAACCCGCATTTTGTGCAGGCCATCGAACAAGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGACGAAGGTGAGCTTCATCCGctt                                                                                                                                      >  2:972481/1‑149 (MQ=255)                tgatCTGCCAGATAACCCGCATTTTGTGCAGGCCATCGAACAAGCCTGGCAACAAATCGTACAATTCGGAGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGACGAAGGTGAGCTTCATCCGctt                                                                                                                                      <  2:1491946/150‑2 (MQ=255)                   tCTGCCAGATAACCCGCATTTTGTGCAGGCCATCGAACAAGCCTGGCAACAAATCGTACAATTCGGCGCTCATCAGGCGCTATTAAACACCCGCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCCCCTTCGTCCGGtctcg                                                                                                                                   >  2:214447/1‑149 (MQ=255)                     tGCCAGATAACCCGCATTTTGTGCAGGCCATCGAACAAGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTcccc                                                                                                                                 >  2:347289/1‑150 (MQ=255)                         aGATAACCCGCATTTTGTGCAGGCCATCGAACAAGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTtct                                                                                                                             <  2:992920/150‑1 (MQ=255)                          gATAACCCGCATTTTGTGCAGGCCATCGAACAAGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTtctc                                                                                                                            <  2:616301/150‑1 (MQ=255)                          gATAACCCGCATTTTGTGCAGGCCATCGAACAAGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTtctc                                                                                                                            <  1:347289/150‑1 (MQ=255)                               cccGCATTTTGTGCAGGCCATCGAACAAGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCGCTTCGCttttg                                                                                                                       >  2:277132/1‑149 (MQ=255)                               cccGCATTTTGTGCAGGCCATCGAACAAGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTc                                                                                                                       >  1:1614055/1‑150 (MQ=255)                                  gCATTTTGTGCAGGCCATCGAACATGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGc                                                                                                                    <  2:1441191/150‑1 (MQ=255)                                  gCATTTTGTGCAGGCCATCGAACAAGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGc                                                                                                                    >  1:85926/1‑150 (MQ=255)                                  gCATTTTGTGCAGGCCATCGAACAAGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGACGAAGGTGAGCTTCATCCg                                                                                                                                         >  1:1583331/1‑129 (MQ=255)                                  gCATTTTGTGCAGGCCATCGAACAAGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGACGAAGGTGAGCTTCATCCg                                                                                                                                         <  2:1583331/129‑1 (MQ=255)                                    aTTTTGTGCAGGCCATCGAACAAGCCTGGCAACAAATCGTACAAAGCGGCGCACATCATTCTCTATTAACCAAACTCAAAATTTAACGATTTCTGAGGTTAAAGAGGATGAAGCTCACCTTCGTCCGCTCTCACCTTCTCTTTTCTGCCt                                                                                                                  <  1:93088/150‑1 (MQ=255)                                     ttttGTGCAGGCCATCGAACAAGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCtt                                                                                                                 <  1:910780/150‑1 (MQ=255)                                       ttGTGCAGGCCATCGAACAAGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCtttt                                                                                                               <  2:1307502/150‑1 (MQ=255)                                         gtgCAGGCCATCGAACAAGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCtttttt                                                                                                             <  1:1456054/150‑1 (MQ=255)                                            cAGGCCATCGAACAAGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGc                                                                                                          >  2:784049/1‑150 (MQ=255)                                            cAGGCCATCGAACAAGCCTGGCAACAAATCGTACAATTCGGCGAACATCCGGCGCTATTAAACACCCTCCAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCGGCTCTCCCATTCTCTTTTCAGCCTTTATTAGc                                                                                                          >  1:293037/1‑150 (MQ=255)                                             aGGCCATCGAACAAGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGcc                                                                                                         <  2:959679/150‑1 (MQ=255)                                              ggCCATCGAACAAGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCa                                                                                                        >  2:7730/1‑150 (MQ=255)                                              ggCCATCGAACAAGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCa                                                                                                        <  2:1344566/150‑1 (MQ=255)                                               gCCATCGATCAAGCCTGGCAACAAATCGTACAATTCTGCGCACATCAGGCGGTATTTTACACCCTCAAAATTTAACGATTTCTGCGGTTTAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAg                                                                                                       <  2:364964/150‑1 (MQ=255)                                               gCCATCGAACAACCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAg                                                                                                       <  2:413713/150‑1 (MQ=255)                                                ccATCGAACAAGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAgg                                                                                                      <  2:528424/150‑1 (MQ=255)                                                 cATCGAACAAGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAGAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGa                                                                                                     >  2:1340366/1‑150 (MQ=255)                                                  aTCGAACAAGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGAt                                                                                                    >  2:1107901/1‑150 (MQ=255)                                                    cGAACAAGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGAAGAAGCTCACCTTCGTCCGCTCTCCCCTACTCTTTTCTGCCTTTTTTAGCCAGGCTTa                                                                                                  >  1:239945/1‑150 (MQ=255)                                                     gAACAAGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTaa                                                                                                 >  2:1181474/1‑150 (MQ=255)                                                      aaCAAGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAAc                                                                                                >  1:15337/1‑150 (MQ=255)                                                      aaCAAGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAAc                                                                                                >  2:42855/1‑150 (MQ=255)                                                         aaGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTATACAACCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCt                                                                                             >  1:668544/1‑150 (MQ=255)                                                          aGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTc                                                                                            >  1:1094120/1‑150 (MQ=255)                                                          aGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTc                                                                                            <  2:91691/150‑1 (MQ=255)                                                           gCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACTCCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCAACTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCa                                                                                           <  1:1018765/150‑1 (MQ=255)                                                            ccTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGTTTAAAGCGGATGAAGCACACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAg                                                                                          <  2:885359/150‑1 (MQ=255)                                                                 caacaaATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAAc                                                                                     <  2:103767/150‑1 (MQ=255)                                                                 caacaaATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAAc                                                                                     <  2:1466911/150‑1 (MQ=255)                                                                  aacaaATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCTCTCTCCCCTTCTCTTTTCTGCCTTTTATAGCCAGGATTAACGCTCAGTTAACt                                                                                    >  2:1652567/1‑150 (MQ=255)                                                                  aacaaATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACt                                                                                    <  1:24614/150‑1 (MQ=255)                                                                     aaaTCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTATGCCTTTTTTATCCAGGATTAACGATCAGTTAACTTAc                                                                                 >  1:1511781/1‑150 (MQ=255)                                                                          gTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCagag                                                                            <  1:1589445/150‑1 (MQ=255)                                                                                  cGGCGCACATCAGGCGCTATTAAACACCCTCAAAATGAAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGGACGCTCTCCCCGTCGCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTAACCTGAGTATTGCgg                                                                    >  1:198773/1‑150 (MQ=255)                                                                                   ggCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGa                                                                   >  2:1420569/1‑150 (MQ=255)                                                                                    gcgcACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAg                                                                  <  2:85926/150‑1 (MQ=255)                                                                                         caTCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGtt                                                             <  2:1614055/150‑1 (MQ=255)                                                                                          aTCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGAGGAGCCGTtg                                                            >  1:923458/1‑150 (MQ=255)                                                                                          aTCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCAGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTtg                                                            <  2:509946/150‑1 (MQ=255)                                                                                            cAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTtgtg                                                          <  1:784049/150‑1 (MQ=255)                                                                                            cAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGCCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTtgtg                                                          <  2:997690/150‑1 (MQ=255)                                                                                             aGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGa                                                         <  2:786391/150‑1 (MQ=255)                                                                                             aGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGa                                                         <  1:135723/150‑1 (MQ=255)                                                                                                            cccTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTAGTGACCAGGACACACCtca                                          >  2:529224/1‑150 (MQ=255)                                                                                                             ccTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCtcat                                         >  2:405662/1‑150 (MQ=255)                                                                                                                cAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGGCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTTCGGAGCCGTTGTGACCAGCACCAACCACATCAc                                      >  2:1591441/1‑150 (MQ=255)                                                                                                                cAAAATTAAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCAc                                      <  1:783272/150‑1 (MQ=255)                                                                                                                 aaaaTTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCAcc                                     <  1:42855/150‑1 (MQ=255)                                                                                                                 aaaaTTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCAcc                                     >  2:1291105/1‑150 (MQ=255)                                                                                                                 aaaaTTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCAcc                                     <  2:15337/150‑1 (MQ=255)                                                                                                                   aaTTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCgg                                   <  1:204036/150‑1 (MQ=255)                                                                                                                        aaCGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTtc                              <  2:447348/150‑1 (MQ=255)                                                                                                                         aCGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTtct                             >  2:1542542/1‑150 (MQ=255)                                                                                                                          cGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTtctc                            <  1:1558603/150‑1 (MQ=255)                                                                                                                                cTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGc                      >  1:953481/1‑150 (MQ=255)                                                                                                                                 cgCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGGATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCg                     <  1:277132/149‑1 (MQ=255)                                                                                                                                   cGGTTCAAGCGGATGAAGCTCACCATCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCCGGATTAACGCTCAGGTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCGCATCACCGGTTTTCTCGGCAGCggg                   <  2:1118710/150‑1 (MQ=255)                                                                                                                                   cGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCggg                   >  2:682902/1‑150 (MQ=255)                                                                                                                                     gTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTTACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGaa                 >  2:193321/1‑150 (MQ=255)                                                                                                                                      ggagaGCGGACGAAGGTGAGCTTCATCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGaaa                >  1:933157/5‑150 (MQ=255)                                                                                                                                         gaGCGGACGAAGGTGAGCTTCATCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACGAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAAcc             >  1:1152118/2‑150 (MQ=255)                                                                                                                                         aaGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAAcc             >  2:939054/1‑150 (MQ=255)                                                                                                                                          aGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTATTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAAGCa            >  1:976977/1‑150 (MQ=255)                                                                                                                                            cGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACg          >  1:992030/1‑150 (MQ=255)                                                                                                                                            cGGACGAAGGTGAGCTTCATCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACg          >  1:420084/1‑150 (MQ=255)                                                                                                                                               aTGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCg       <  2:923458/150‑1 (MQ=255)                                                                                                                                               aTGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCg       <  1:1181474/150‑1 (MQ=255)                                                                                                                                                  aaGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGAtt    >  1:381340/1‑150 (MQ=255)                                                                                                                                                    gCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGAttct  >  1:437519/1‑150 (MQ=255)                                                                                                                                                     |                                                                                                                                                     TGAAGTTTTCGGTGATGATCTGCCAGATAACCCGCATTTTGTGCAGGCCATCGAACAAGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCT  >  NC_000913/2267591‑2267887

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑