Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,267,748	G→A	8.0%	intergenic (+37/‑81)	yeiQ → / → yeiR	putative NAD‑dependent D‑mannonate oxidoreductase/Zn‑stimulated GTPase involved in zinc homeostasis; mutants are cadmium and EDTA sensitive; Zn(2+) binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,267,748	0	G	A	8.0%	263.1 / 6.9	87	intergenic (+37/‑81)	yeiQ/yeiR	putative NAD‑dependent D‑mannonate oxidoreductase/Zn‑stimulated GTPase involved in zinc homeostasis; mutants are cadmium and EDTA sensitive; Zn(2+) binding protein
Reads supporting (aligned to +/- strand):  ref base G (39/41);  new base A (5/2);  total (44/43)
Fisher's exact test for biased strand distribution p-value = 4.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGTGATGATCTGCCAGATAACCCGCATTTTGTGCAGGCCATCGAACAAGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTT  >  NC_000913/2267601‑2267896                                                                                                                                                    |                                                                                                                                                     ggTGATGATCTGCCAGATAACCCGCATTTTGTGCAGGTCATCGAACAAGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTc                                                                                                                                                    <  1:490477/150‑1 (MQ=255)  gTGATGATCTGCCAGATAACCCGCATTTTGTGCAGGCCATCGAACAAGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTcc                                                                                                                                                   <  2:519039/150‑1 (MQ=255)      tgatCTGCCAGATAACCCGCATTTTGTGCAGGCCATCGAACAAGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGctc                                                                                                                                               <  1:915145/150‑1 (MQ=255)      tgatCTGCCAGATAACCCGCATTTTGTGCAGGCCATCGAACAAGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGACGAAGGTGAGCTTCATCCGctt                                                                                                                                               >  2:972481/1‑149 (MQ=255)      tgatCTGCCAGATAACCCGCATTTTGTGCAGGCCATCGAACAAGCCTGGCAACAAATCGTACAATTCGGAGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGACGAAGGTGAGCTTCATCCGctt                                                                                                                                               <  2:1491946/150‑2 (MQ=255)         tCTGCCAGATAACCCGCATTTTGTGCAGGCCATCGAACAAGCCTGGCAACAAATCGTACAATTCGGCGCTCATCAGGCGCTATTAAACACCCGCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCCCCTTCGTCCGGtctcg                                                                                                                                            >  2:214447/1‑149 (MQ=255)           tGCCAGATAACCCGCATTTTGTGCAGGCCATCGAACAAGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTcccc                                                                                                                                          >  2:347289/1‑150 (MQ=255)               aGATAACCCGCATTTTGTGCAGGCCATCGAACAAGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTtct                                                                                                                                      <  2:992920/150‑1 (MQ=255)                gATAACCCGCATTTTGTGCAGGCCATCGAACAAGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTtctc                                                                                                                                     <  1:347289/150‑1 (MQ=255)                gATAACCCGCATTTTGTGCAGGCCATCGAACAAGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTtctc                                                                                                                                     <  2:616301/150‑1 (MQ=255)                     cccGCATTTTGTGCAGGCCATCGAACAAGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCGCTTCGCttttg                                                                                                                                >  2:277132/1‑149 (MQ=255)                     cccGCATTTTGTGCAGGCCATCGAACAAGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTc                                                                                                                                >  1:1614055/1‑150 (MQ=255)                        gCATTTTGTGCAGGCCATCGAACATGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGc                                                                                                                             <  2:1441191/150‑1 (MQ=255)                        gCATTTTGTGCAGGCCATCGAACAAGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGc                                                                                                                             >  1:85926/1‑150 (MQ=255)                        gCATTTTGTGCAGGCCATCGAACAAGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGACGAAGGTGAGCTTCATCCg                                                                                                                                                  >  1:1583331/1‑129 (MQ=255)                        gCATTTTGTGCAGGCCATCGAACAAGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGACGAAGGTGAGCTTCATCCg                                                                                                                                                  <  2:1583331/129‑1 (MQ=255)                          aTTTTGTGCAGGCCATCGAACAAGCCTGGCAACAAATCGTACAAAGCGGCGCACATCATTCTCTATTAACCAAACTCAAAATTTAACGATTTCTGAGGTTAAAGAGGATGAAGCTCACCTTCGTCCGCTCTCACCTTCTCTTTTCTGCCt                                                                                                                           <  1:93088/150‑1 (MQ=255)                           ttttGTGCAGGCCATCGAACAAGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCtt                                                                                                                          <  1:910780/150‑1 (MQ=255)                             ttGTGCAGGCCATCGAACAAGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCtttt                                                                                                                        <  2:1307502/150‑1 (MQ=255)                               gtgCAGGCCATCGAACAAGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCtttttt                                                                                                                      <  1:1456054/150‑1 (MQ=255)                                  cAGGCCATCGAACAAGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGc                                                                                                                   >  2:784049/1‑150 (MQ=255)                                  cAGGCCATCGAACAAGCCTGGCAACAAATCGTACAATTCGGCGAACATCCGGCGCTATTAAACACCCTCCAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCGGCTCTCCCATTCTCTTTTCAGCCTTTATTAGc                                                                                                                   >  1:293037/1‑150 (MQ=255)                                   aGGCCATCGAACAAGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGcc                                                                                                                  <  2:959679/150‑1 (MQ=255)                                    ggCCATCGAACAAGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCa                                                                                                                 <  2:1344566/150‑1 (MQ=255)                                    ggCCATCGAACAAGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCa                                                                                                                 >  2:7730/1‑150 (MQ=255)                                     gCCATCGATCAAGCCTGGCAACAAATCGTACAATTCTGCGCACATCAGGCGGTATTTTACACCCTCAAAATTTAACGATTTCTGCGGTTTAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAg                                                                                                                <  2:364964/150‑1 (MQ=255)                                     gCCATCGAACAACCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAg                                                                                                                <  2:413713/150‑1 (MQ=255)                                      ccATCGAACAAGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAgg                                                                                                               <  2:528424/150‑1 (MQ=255)                                       cATCGAACAAGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAGAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGa                                                                                                              >  2:1340366/1‑150 (MQ=255)                                        aTCGAACAAGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGAt                                                                                                             >  2:1107901/1‑150 (MQ=255)                                          cGAACAAGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGAAGAAGCTCACCTTCGTCCGCTCTCCCCTACTCTTTTCTGCCTTTTTTAGCCAGGCTTa                                                                                                           >  1:239945/1‑150 (MQ=255)                                           gAACAAGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTaa                                                                                                          >  2:1181474/1‑150 (MQ=255)                                            aaCAAGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAAc                                                                                                         >  2:42855/1‑150 (MQ=255)                                            aaCAAGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAAc                                                                                                         >  1:15337/1‑150 (MQ=255)                                               aaGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTATACAACCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCt                                                                                                      >  1:668544/1‑150 (MQ=255)                                                aGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTc                                                                                                     >  1:1094120/1‑150 (MQ=255)                                                aGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTc                                                                                                     <  2:91691/150‑1 (MQ=255)                                                 gCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACTCCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCAACTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCa                                                                                                    <  1:1018765/150‑1 (MQ=255)                                                  ccTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGTTTAAAGCGGATGAAGCACACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAg                                                                                                   <  2:885359/150‑1 (MQ=255)                                                       caacaaATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAAc                                                                                              <  2:103767/150‑1 (MQ=255)                                                       caacaaATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAAc                                                                                              <  2:1466911/150‑1 (MQ=255)                                                        aacaaATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCTCTCTCCCCTTCTCTTTTCTGCCTTTTATAGCCAGGATTAACGCTCAGTTAACt                                                                                             >  2:1652567/1‑150 (MQ=255)                                                        aacaaATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACt                                                                                             <  1:24614/150‑1 (MQ=255)                                                           aaaTCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTATGCCTTTTTTATCCAGGATTAACGATCAGTTAACTTAc                                                                                          >  1:1511781/1‑150 (MQ=255)                                                                gTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCagag                                                                                     <  1:1589445/150‑1 (MQ=255)                                                                        cGGCGCACATCAGGCGCTATTAAACACCCTCAAAATGAAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGGACGCTCTCCCCGTCGCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTAACCTGAGTATTGCgg                                                                             >  1:198773/1‑150 (MQ=255)                                                                         ggCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGa                                                                            >  2:1420569/1‑150 (MQ=255)                                                                          gcgcACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAg                                                                           <  2:85926/150‑1 (MQ=255)                                                                               caTCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGtt                                                                      <  2:1614055/150‑1 (MQ=255)                                                                                aTCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGAGGAGCCGTtg                                                                     >  1:923458/1‑150 (MQ=255)                                                                                aTCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCAGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTtg                                                                     <  2:509946/150‑1 (MQ=255)                                                                                  cAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTtgtg                                                                   <  1:784049/150‑1 (MQ=255)                                                                                  cAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGCCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTtgtg                                                                   <  2:997690/150‑1 (MQ=255)                                                                                   aGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGa                                                                  <  2:786391/150‑1 (MQ=255)                                                                                   aGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGa                                                                  <  1:135723/150‑1 (MQ=255)                                                                                                  cccTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTAGTGACCAGGACACACCtca                                                   >  2:529224/1‑150 (MQ=255)                                                                                                   ccTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCtcat                                                  >  2:405662/1‑150 (MQ=255)                                                                                                      cAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGGCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTTCGGAGCCGTTGTGACCAGCACCAACCACATCAc                                               >  2:1591441/1‑150 (MQ=255)                                                                                                      cAAAATTAAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCAc                                               <  1:783272/150‑1 (MQ=255)                                                                                                       aaaaTTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCAcc                                              <  1:42855/150‑1 (MQ=255)                                                                                                       aaaaTTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCAcc                                              >  2:1291105/1‑150 (MQ=255)                                                                                                       aaaaTTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCAcc                                              <  2:15337/150‑1 (MQ=255)                                                                                                         aaTTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCgg                                            <  1:204036/150‑1 (MQ=255)                                                                                                              aaCGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTtc                                       <  2:447348/150‑1 (MQ=255)                                                                                                               aCGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTtct                                      >  2:1542542/1‑150 (MQ=255)                                                                                                                cGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTtctc                                     <  1:1558603/150‑1 (MQ=255)                                                                                                                      cTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGc                               >  1:953481/1‑150 (MQ=255)                                                                                                                       cgCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGGATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCg                              <  1:277132/149‑1 (MQ=255)                                                                                                                         cGGTTCAAGCGGATGAAGCTCACCATCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCCGGATTAACGCTCAGGTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCGCATCACCGGTTTTCTCGGCAGCggg                            <  2:1118710/150‑1 (MQ=255)                                                                                                                         cGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCggg                            >  2:682902/1‑150 (MQ=255)                                                                                                                           gTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTTACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGaa                          >  2:193321/1‑150 (MQ=255)                                                                                                                            ggagaGCGGACGAAGGTGAGCTTCATCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGaaa                         >  1:933157/5‑150 (MQ=255)                                                                                                                               gaGCGGACGAAGGTGAGCTTCATCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACGAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAAcc                      >  1:1152118/2‑150 (MQ=255)                                                                                                                               aaGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAAcc                      >  2:939054/1‑150 (MQ=255)                                                                                                                                aGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTATTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAAGCa                     >  1:976977/1‑150 (MQ=255)                                                                                                                                  cGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACg                   >  1:992030/1‑150 (MQ=255)                                                                                                                                  cGGACGAAGGTGAGCTTCATCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACg                   >  1:420084/1‑150 (MQ=255)                                                                                                                                     aTGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCg                <  2:923458/150‑1 (MQ=255)                                                                                                                                     aTGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCg                <  1:1181474/150‑1 (MQ=255)                                                                                                                                        aaGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGAtt             >  1:381340/1‑150 (MQ=255)                                                                                                                                          gCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGAttct           >  1:437519/1‑150 (MQ=255)                                                                                                                                            tCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTTCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGAttcttc         <  2:1518181/150‑1 (MQ=255)                                                                                                                                            tCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAACCCCCGTCGAttcttc         >  2:291521/1‑150 (MQ=255)                                                                                                                                             cACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGCCCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCa        >  2:534728/1‑150 (MQ=255)                                                                                                                                              aCCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCAt       >  1:172965/1‑150 (MQ=255)                                                                                                                                               ccTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCACCCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGAGTCTTCAta      >  2:527676/1‑149 (MQ=255)                                                                                                                                                   cGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCACCCTCATCACCGGTTTTATCGGAAGCGGGAAAACCACGTCGATTCTTCATCTGtt  >  1:879128/1‑150 (MQ=255)                                                                                                                                                    |                                                                                                                                                     GGTGATGATCTGCCAGATAACCCGCATTTTGTGCAGGCCATCGAACAAGCCTGGCAACAAATCGTACAATTCGGCGCACATCAGGCGCTATTAAACACCCTCAAAATTTAACGATTTCTGCGGTTAAAGCGGATGAAGCTCACCTTCGTCCGCTCTCCCCTTCTCTTTTCTGCCTTTTTTAGCCAGGATTAACGCTCAGTTAACTTACCAGAGTATTGCGGAGCCGTTGTGACCAGGACCAACCTCATCACCGGTTTTCTCGGCAGCGGGAAAACCACGTCGATTCTTCATCTGTT  >  NC_000913/2267601‑2267896

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑