Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 2,270,602 | T→C | 7.8% | intergenic (+57/‑124) | mepS → / → rtn | murein DD‑endopeptidase, space‑maker hydrolase, mutational suppressor of prc thermosensitivity, outer membrane lipoprotein, weak murein LD‑carboxypeptidase/resistance protein for phages lambda and N4, putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,270,602 | 0 | T | C | 7.8% | 322.0 / 14.9 | 115 | intergenic (+57/‑124) | mepS/rtn | murein DD‑endopeptidase, space‑maker hydrolase, mutational suppressor of prc thermosensitivity, outer membrane lipoprotein, weak murein LD‑carboxypeptidase/resistance protein for phages lambda and N4, putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase |
Reads supporting (aligned to +/- strand): ref base T (53/53); new base C (3/6); total (56/59) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 4.91e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.45e-01 |
Only 100 of 115 total aligned reads displayed.
CCAGCAGTGGTGTTATTATTTCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTG > NC_000913/2270457‑2270750 | ccaGCAGTGGTGTTATTATTTCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAgg > 2:1220043/1‑150 (MQ=255) gcagTGGTGTTATTATTTCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAg < 1:1134541/150‑1 (MQ=255) cagTGGTGTTATTATTTCCAGCATGAATGAACCGTACTTGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGt < 2:1383504/150‑1 (MQ=255) agTGGTGTTATTATTTCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTg < 2:26754/150‑1 (MQ=255) ggtgTTATTATTTCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑tt > 2:1265076/1‑150 (MQ=255) ggtgTTATTATTTCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTATGGAAGCAATCCCTTGGCTATCCTGAAGAGATAACTGAAAGCACTGCTTAGGCAGTGC‑tt > 1:1034335/1‑150 (MQ=255) ggtgTTATTATTTCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGACGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGAAGCAATCCCTTGGCTATCCTGATGAGTTAACTGAAAGCAATGCTTAGGCACTGC‑tt > 1:306691/1‑150 (MQ=255) tgtTATTATTTCCAGCATGAATGAAACGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCCTAAGCAGTGC‑tttc < 1:832756/150‑2 (MQ=255) tgtAATTATTTCCAGCATGAATGAACCGTACTGGAAGACGCGCTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑tttt < 1:1539168/150‑1 (MQ=255) gtTATTATTTCCAGCATGAATGAACCGTACTGTAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCCTAAGCAGTGC‑tttca < 1:345293/150‑3 (MQ=255) gtTATTATTTCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑ttttt < 1:1034340/150‑1 (MQ=255) gtTATTATTTCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCCTAAGCAGTGC‑tttca < 1:1662262/150‑3 (MQ=255) ttattaTTTCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCCTAAGCAGTGC‑tttcag < 2:656887/150‑4 (MQ=255) tattatTTCCAGGATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCCTAAGCAGTGC‑tttcagt < 2:761267/150‑5 (MQ=255) tattatTTCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTg > 2:577403/1‑150 (MQ=255) tattatTTCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTg < 2:899376/150‑1 (MQ=255) tattTCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTGCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGttt < 1:1287656/150‑1 (MQ=255) attTCCAGCATGAATGAACCGTACTGGAATAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGtttt < 1:743255/150‑1 (MQ=255) tttCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTttc < 1:1352348/150‑1 (MQ=255) tttCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTttc < 2:123216/150‑1 (MQ=255) tCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTttcat > 1:251144/1‑150 (MQ=255) cAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTttcattc > 1:270954/1‑150 (MQ=255) aGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTttcattca > 2:1292670/1‑150 (MQ=255) gCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTtcattcat > 1:446986/1‑150 (MQ=255) cATGAATGAACCGTACTTGAAGAAGCGTTACATCGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATtcatc < 1:316416/150‑1 (MQ=255) tgaatgaaCCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCag < 2:1614034/150‑1 (MQ=255) tgaatgaaCCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTATTCATTCATCag > 1:9977/1‑150 (MQ=255) gaatgaaCCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCaga > 2:117203/1‑150 (MQ=255) aatgaaCCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCagag > 1:299005/1‑150 (MQ=255) aatgaaCCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCagag < 1:88570/150‑1 (MQ=255) atgaaCCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAgaga > 2:1590314/1‑150 (MQ=255) atgaaCCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAgaga > 1:844000/1‑150 (MQ=255) atgaaCCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAgaga < 1:1140700/150‑1 (MQ=255) tgaaCCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGaa < 1:985683/150‑1 (MQ=255) gaaCCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGaaa > 1:1548351/1‑150 (MQ=255) aaCCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGaaaa < 1:1669733/150‑1 (MQ=255) aaCCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGaaaa > 1:1549333/1‑150 (MQ=255) aCTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCCGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGt > 2:198008/1‑150 (MQ=255) ttgaagaaGCGTTACTACGAAGCACGCCGGTTTCTCAGCCGCTGCTTATAAACCGTTTGGATGCAATTCCTTGGCTTTACTGTCTAGTTATCTGAAAGCACTGCTTAGTCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGttt < 1:15401/148‑1 (MQ=255) tGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGttt > 2:450968/1‑150 (MQ=255) ggAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGttta > 1:227994/1‑149 (MQ=255) aagaagCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCcg > 1:1453234/1‑150 (MQ=255) agaagCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCcgc > 2:865739/1‑150 (MQ=255) agaagCGTTACAACGAAGAACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCcgc < 1:1265076/150‑1 (MQ=255) gaagCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCcgcg < 2:884519/150‑1 (MQ=255) gCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCt > 1:545516/1‑150 (MQ=255) cGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCtt < 2:1269486/150‑1 (MQ=255) gTTACAACGAAGCACGCCGGGTTCTCAGCCGCTTCTTATAAACCGTTTGGATTCAATCCCTTGGCTATCCTGAGGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTTGTTTTCATTCATCAGAGAATATGATGTTTGCGCGTCtt < 2:122478/150‑1 (MQ=255) aCGAAGCAGGCCGGGTTCTCAGCCGCAGGTAATAAACCGTTTGGATGCAATCCCTTGGCTATACTGTCGAGTTAACTGAAAGCACTGGTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTTATCCAg < 2:800426/150‑1 (MQ=255) aCGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAg < 2:188954/150‑1 (MQ=255) gAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGc < 1:1220043/150‑1 (MQ=255) gCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCCGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTc < 1:198008/150‑1 (MQ=255) gTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTc > 2:232795/1‑150 (MQ=255) tctcAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCAt > 2:1534286/1‑150 (MQ=255) cAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGt > 1:1561181/1‑150 (MQ=255) gCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCt > 1:373043/1‑150 (MQ=255) aGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCttt > 2:1624839/1‑150 (MQ=255) aGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCttt > 2:1482874/1‑150 (MQ=255) gCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCtttt < 1:480747/150‑1 (MQ=255) gCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCtttt < 1:1636008/150‑1 (MQ=255) cTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTa < 1:1703400/150‑1 (MQ=255) taaACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAAtg < 2:935260/150‑1 (MQ=255) aaCCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAAtgtt < 1:486410/150‑1 (MQ=255) aaCCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAAtgtt > 2:830145/1‑150 (MQ=255) aaCCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAAtgtt > 2:1711117/1‑150 (MQ=255) aaCCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGCCCGGTCATTGTTATCTTTTAAAtgtt > 1:1469425/1‑150 (MQ=255) ccGTTTGGATGCAATCCCTTGGGTATCCTGTCGAGTTAACTGAAAGCAGTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCACGCTATATTCCTGTCATTGTTATCTTTTAAAtgttgt < 2:503064/150‑1 (MQ=255) ccGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAAtgttgt < 2:711156/150‑1 (MQ=255) ttGGATGCAATCCCTTTGCTAACCTGACGAGATAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGGCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTa < 1:1061500/150‑1 (MQ=255) ggATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAAt > 2:1051341/1‑150 (MQ=255) gCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCa < 1:1292670/150‑1 (MQ=255) cAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCTTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAg > 2:601172/1‑150 (MQ=255) cAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAg < 2:1455993/150‑1 (MQ=255) cAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAg < 1:594696/150‑1 (MQ=255) cAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGAAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAg > 2:1460050/1‑150 (MQ=255) tCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGaa > 2:856193/1‑150 (MQ=255) ccTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGATTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAAt > 2:35165/1‑150 (MQ=255) gCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACg < 2:270954/150‑1 (MQ=255) gCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACg < 2:1548351/150‑1 (MQ=255) cTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACgg > 2:1633221/1‑150 (MQ=255) tCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATTTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAAt < 1:333217/150‑1 (MQ=255) tCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAAt < 1:1352109/150‑1 (MQ=255) gACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAgg > 1:1421984/1‑133 (MQ=255) gACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAgg < 2:1421984/133‑1 (MQ=255) gACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATg > 2:1176515/1‑150 (MQ=255) gAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTc < 2:1590069/150‑1 (MQ=255) aGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCa < 1:450968/150‑1 (MQ=255) aGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCa > 2:402272/1‑150 (MQ=255) aGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCa > 2:447078/1‑150 (MQ=255) gTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCAt > 1:70969/1‑150 (MQ=255) tAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATAc < 2:1372237/150‑1 (MQ=255) tAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATAc > 1:1709485/1‑150 (MQ=255) tAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTAATCAGAGAAAATGATGTTTCCGCTTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATAc > 2:1478668/1‑150 (MQ=255) acaaaaaaGCACTGCCTAAGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATAcg > 1:131287/6‑150 (MQ=255) caaaaaaGCACTGCCTAAGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATAcgc > 2:569478/5‑150 (MQ=255) caaaaaaGCACTGCCTAAGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATAcgc > 1:945612/5‑150 (MQ=255) aCTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCGGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATAcgc > 2:921401/1‑150 (MQ=255) gAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCgg > 1:597532/1‑85 (MQ=255) aGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCACTccc > 2:75373/1‑150 (MQ=255) cTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCGGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTg < 1:921401/150‑1 (MQ=255) | CCAGCAGTGGTGTTATTATTTCCAGCATGAATGAACCGTACTGGAAGAAGCGTTACAACGAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGC‑TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTG > NC_000913/2270457‑2270750 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |