Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,373,177 G→A 8.8% G96S (GGC→AGC)  arnF → undecaprenyl phosphate‑alpha‑L‑ara4N exporter; flippase ArnEF subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,373,1770GA 8.8% 235.1 / 7.5 79G96S (GGC→AGC) arnFundecaprenyl phosphate‑alpha‑L‑ara4N exporter; flippase ArnEF subunit
Reads supporting (aligned to +/- strand):  ref base G (39/33);  new base A (3/4);  total (42/37)
Fisher's exact test for biased strand distribution p-value = 7.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.99e-01

TGCCCGGATACTGTTACTCGGGCTGCTGGGTTATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGTTCCCACTCATCGGGGCTAT  >  NC_000913/2373029‑2373324
                                                                                                                                                    |                                                                                                                                                   
tGCCCGGATACTGTTACTCGGGCTGCTGGGTTATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCgg                                                                                                                                                    <  2:1322428/150‑1 (MQ=255)
  cccGGATACTGTTACTCGGGCTGCTGGGTTATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCt                                                                                                                                                  >  1:456914/1‑150 (MQ=255)
        tACTGTTACTCGGGCTGCTGGGTTATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTg                                                                                                                                                 <  1:1567664/145‑1 (MQ=255)
        tACTGTTACTCGGGCTGCTGGGTTATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTg                                                                                                                                                 >  2:1567664/1‑145 (MQ=255)
        tACTGTTACTCGGGCTGCTGGGTTATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAg                                                                                                                                            <  1:319133/150‑1 (MQ=255)
         aCTGTTACTCGGGCTGCTGGGTTATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAgg                                                                                                                                           <  2:1102448/150‑1 (MQ=255)
         aCTGTTACTCGGGCTGCTGGGTTATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAgg                                                                                                                                           <  1:445280/150‑1 (MQ=255)
         aCTGTTACTCGGGCTGCTGGGTTATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAgg                                                                                                                                           <  2:1536668/150‑1 (MQ=255)
           tGTTACTCGGGCTGCTGGGTTATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCAGCCGGGAAgtac                                                                                                                                         <  2:1165691/150‑4 (MQ=255)
            gTTACTCGGGCTGCTGGGTTATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCAGCCGGGAAgtacc                                                                                                                                        <  2:1441786/150‑5 (MQ=255)
            gTTACTCGGGATGCTGGGTTATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGATATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCAGCCGGGAAgtacc                                                                                                                                        <  1:376686/150‑5 (MQ=255)
               aCTCGGGCTGCTGGGTTATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCAGCCGGGAAgtaccatt                                                                                                                                     <  1:964597/150‑8 (MQ=255)
                   gggCTGCTGGGTTATCTGCTTTCCGTTTTTTGATGGTAAAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCTGGCTGGGAAGGGACTTTTTCg                                                                                                                                 <  2:843103/150‑1 (MQ=255)
                    ggCTGCTGGGTTATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGc                                                                                                                                <  1:1012371/150‑1 (MQ=255)
                      ctgctgGGTTATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTg                                                                                                                              >  1:83690/1‑150 (MQ=255)
                       tgctgGGTTATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGGCTTTTTCGCTGa                                                                                                                             >  1:1616880/1‑150 (MQ=255)
                       tgctgGGTTATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGa                                                                                                                             >  1:508598/1‑150 (MQ=255)
                           gggTTATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGc                                                                                                                         >  2:295112/1‑150 (MQ=255)
                           gggTTATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGc                                                                                                                         >  2:659979/1‑150 (MQ=255)
                                      tttCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAg                                                                                                              <  2:1459222/150‑1 (MQ=255)
                                         ccGTTTTTTGTTGGTATAACACGCTTCATAAGCTTGCGCTACGCAAAGCCTATGCCTTGCGCAGTATGAGTTATGGGCGGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAg                                                                                                           <  2:1452821/150‑1 (MQ=255)
                                         ccGGTTTTTGGTGCTATAAAACGCTTCATAAACTTGCCCTTATCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAg                                                                                                           <  2:1648519/150‑1 (MQ=255)
                                           gTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGcc                                                                                                         >  2:1245101/1‑150 (MQ=255)
                                           gTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAACACTACTGGGAGTAGcc                                                                                                         >  2:1695626/1‑150 (MQ=255)
                                                  gttgGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGtatta                                                                                                  >  1:1323061/1‑150 (MQ=255)
                                                    tgGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATg                                                                                                <  2:345683/150‑1 (MQ=255)
                                                    tgGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATg                                                                                                <  2:426673/150‑1 (MQ=255)
                                                           aaaCGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGt                                                                                         >  2:1214712/1‑150 (MQ=255)
                                                            aaCGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGtt                                                                                        >  1:625414/1‑150 (MQ=255)
                                                                cTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATg                                                                                    <  1:295112/150‑1 (MQ=255)
                                                                     tAATCTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGTTTGCTTCTATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGAt                                                                               <  1:589051/150‑1 (MQ=255)
                                                                         cTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCAGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGAGGGTTGATGCTGATTTTc                                                                           >  1:96530/1‑150 (MQ=255)
                                                                          ttGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTTCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTcc                                                                          <  1:1133658/150‑1 (MQ=255)
                                                                           tGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCt                                                                         >  2:732388/1‑150 (MQ=255)
                                                                             cgcTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGc                                                                       <  2:1000073/150‑1 (MQ=255)
                                                                             cgcTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGc                                                                       <  1:659979/150‑1 (MQ=255)
                                                                               caaaGCAAAGCCGATTCCGTGCGCAGGATGAGTTATGTTCAGGTATTGATTTCTTCAAATGTACTTACCGGCTGGGAAGGGACTTTTTAGCTGAAAGCCCGAATGGGAGTAGCCGGTATTATTAGCCGGGTGAATCGGAATTTCCTAccc                                                                     >  2:700711/3‑150 (MQ=255)
                                                                                tgaGCAAGGCATAGGCTTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCa                                                                    >  1:682630/3‑150 (MQ=255)
                                                                                tAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACAACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCa                                                                    >  2:463801/1‑150 (MQ=255)
                                                                                 aaGCAAAGCCTATGCCGTGCTCAGTATGAGTTAGGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCGGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCAc                                                                   <  2:823608/150‑1 (MQ=255)
                                                                                        gCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGCGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACaaaa                                                            >  2:683716/1‑150 (MQ=255)
                                                                                         ccTATGCCTTGCTCAGTATGAGTTATGGGCGGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAaac                                                           <  2:1501561/150‑1 (MQ=255)
                                                                                           tATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAaacaa                                                         >  2:736798/1‑150 (MQ=255)
                                                                                                cTTTCTCAGTATGAGTTATGTGCTGGTTTGGATTGCTTCAATGGTAGTTCCCGGCTGGGATGTGACTTTTTCGCTGAAAGCACTACTGGTAGTATCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTa                                                    <  1:1302489/150‑1 (MQ=255)
                                                                                                 ttGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTAc                                                   <  1:736798/150‑1 (MQ=255)
                                                                                                 ttGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCACAAGACAAAACAACGTTAc                                                   >  1:60672/1‑150 (MQ=255)
                                                                                                   gCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTg                                                 >  1:455711/1‑150 (MQ=255)
                                                                                                    cTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGa                                                >  2:768800/1‑150 (MQ=255)
                                                                                                      cAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGt                                              >  1:1654244/1‑150 (MQ=255)
                                                                                                      cAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGt                                              >  1:202263/1‑150 (MQ=255)
                                                                                                        gTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGttt                                            >  1:1702339/1‑150 (MQ=255)
                                                                                                         tATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTcc                                                                          <  1:199877/119‑1 (MQ=255)
                                                                                                         tATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTcc                                                                          >  2:199877/1‑119 (MQ=255)
                                                                                                           tGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTcc                                         <  2:508598/150‑1 (MQ=255)
                                                                                                               ttATGTGCTGGTAGGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGc                                     <  2:1280640/150‑1 (MQ=255)
                                                                                                                tATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGcc                                    <  2:1323061/150‑1 (MQ=255)
                                                                                                                  tgtgCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTTCTGAGTTTTCCCTGCCAc                                  >  2:1355601/1‑150 (MQ=255)
                                                                                                                     gCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAg                                               >  1:1676900/1‑134 (MQ=255)
                                                                                                                     gCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAg                                               <  2:1676900/134‑1 (MQ=255)
                                                                                                                        ggTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTaca                            >  1:1580437/1‑150 (MQ=255)
                                                                                                                               aTTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAaaca                                                          <  1:719828/113‑1 (MQ=255)
                                                                                                                               aTTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAaaca                                                          >  2:719828/1‑113 (MQ=255)
                                                                                                                               aTTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAaaca                                                          >  2:457394/1‑113 (MQ=255)
                                                                                                                               aTTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAaaca                                                          <  1:457394/113‑1 (MQ=255)
                                                                                                                                  gCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGTTcc                  >  2:193234/1‑150 (MQ=255)
                                                                                                                                   cTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGTTccc                 <  2:456914/150‑1 (MQ=255)
                                                                                                                                   cTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGTTccc                 >  1:608746/1‑150 (MQ=255)
                                                                                                                                   cTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGTTccc                 >  1:917208/1‑150 (MQ=255)
                                                                                                                                   cTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACAACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGTTccc                 <  1:463801/150‑1 (MQ=255)
                                                                                                                                    ttCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTACCGTTCCCa                <  2:682630/150‑1 (MQ=255)
                                                                                                                                        aagtcccTTCCCAGCCGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGTTCCCACTCa            >  1:612177/7‑150 (MQ=255)
                                                                                                                                        aTGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTACTGCCAAAGACAAAACAACGTTACTGAGTTTTCCCTTCAACTTTACACTGCAGTTCCCACTCa            >  1:1174447/1‑150 (MQ=255)
                                                                                                                                          gtcccTTCCCAGCCGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGCGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGATTTCCCTGCCACTTTACACTGCCGTTCACAGTCATc          >  1:7836/5‑150 (MQ=255)
                                                                                                                                           gTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGTTCCCACTCATCg         <  2:83690/150‑1 (MQ=255)
                                                                                                                                           gTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGTTCCCACTCATCg         >  1:643774/1‑150 (MQ=255)
                                                                                                                                               ttCCCAGCCGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGTTCCCACTCATCGGGGc     >  1:1547871/1‑150 (MQ=255)
                                                                                                                                                tCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGTTCCCACTCATCGGGGCt    >  1:957966/1‑150 (MQ=255)
                                                                                                                                                  ccGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGTTCCCACTCATCGGGGCtat  >  1:1237175/1‑150 (MQ=255)
                                                                                                                                                  ccGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGTTCCCACTCATCGGGGCt    >  2:1422007/1‑148 (MQ=255)
                                                                                                                                                  ccGGCGGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGTTCCCACTCATCGGGGCt    <  1:1422007/148‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TGCCCGGATACTGTTACTCGGGCTGCTGGGTTATCTGCTTTCCGTTTTTTGTTGGTATAAAACGCTTCATAAACTTGCGCTAAGCAAAGCCTATGCCTTGCTCAGTATGAGTTATGTGCTGGTATGGATTGCTTCAATGGTACTTCCCGGCTGGGAAGGGACTTTTTCGCTGAAAGCACTACTGGGAGTAGCCTGTATTATGAGCGGGTTGATGCTGATTTTCCTGCCCACGACAAAACAACGTTACTGAGTTTTCCCTGCCACTTTACACTGCCGTTCCCACTCATCGGGGCTAT  >  NC_000913/2373029‑2373324

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: