Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,383,956 A→T 6.0% intergenic (+32/+39) elaD → / ← yfbK protease, capable of cleaving an AMC‑ubiquitin model substrate/Von Willebrand factor domain putative lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,383,9560AT 6.0% 394.8 / 10.0 133intergenic (+32/+39)elaD/yfbKprotease, capable of cleaving an AMC‑ubiquitin model substrate/Von Willebrand factor domain putative lipoprotein
Reads supporting (aligned to +/- strand):  ref base A (62/63);  new base T (3/5);  total (65/68)
Fisher's exact test for biased strand distribution p-value = 7.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.73e-01

Only 100 of 133 total aligned reads displayed.

CAGAAATTGTTATTTAGATAAATATGGCGATGCAAATATCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCTGTGGATCTTCCCCTTTTGCCTGCTGAGCCCACTGTTTGATC  >  NC_000913/2383810‑2384103
                                                                                                                                                   |                                                                                                                                                   
cAGAAATTGTTATTTAGATAAATATGGCGATGCAAATATCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAAAAGAGCAACCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGACAGCAAata                                                                                                                                                  >  2:1637208/1‑150 (MQ=255)
   aaaTTGTTATTTAGATAAATATGGCGATGCAAATATCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGc                                                                                                                                               <  2:1437507/150‑1 (MQ=255)
     aTTGTTATTTAGATAAATATGGCGATGCAAATATCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTg                                                                                                                                             >  1:16011/1‑150 (MQ=255)
      ttGTTATTTAGATAAATATGGCGATGCAAATATCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGa                                                                                                                                            <  2:1350317/150‑1 (MQ=255)
         ttATTTAGATAAATATGGCGATGCAAATATCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCATATTTGCTGACTg                                                                                                                                         <  2:580578/150‑1 (MQ=255)
         ttATTTAGATAAATATGGCGATGCAAATATCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTg                                                                                                                                         >  2:1577845/1‑150 (MQ=255)
         ttATTTAGATAAATATGGCGATGCAAATATCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTg                                                                                                                                         <  2:371345/150‑1 (MQ=255)
          tATTTAGATAAATATGGCGATGCAAATATCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTgg                                                                                                                                        <  1:1711744/150‑1 (MQ=255)
          tATTTAGATAAATATGGCGATGCAAATATCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTgg                                                                                                                                        >  1:1603406/1‑150 (MQ=255)
           aTTTAGATAAATATGGCGATGCAAATATCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCATATTTGCTGACTGGt                                                                                                                                       <  1:870703/150‑1 (MQ=255)
           aTTTAGATAAATATGGCGATGCAAATATCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGt                                                                                                                                       <  2:1279550/150‑1 (MQ=255)
            tttAGATAAATATGGCGATGCAAATATCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTg                                                                                                                                             <  1:1088261/143‑1 (MQ=255)
            tttAGATAAATATGGCGATGCAAATATCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTc                                                                                                                                      <  2:1431426/150‑1 (MQ=255)
            tttAGATAAATATGGCGATGCAAATATCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTc                                                                                                                                      <  2:1438017/150‑1 (MQ=255)
             ttAGATAAATATGGCGATGCAAATATCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCg                                                                                                                                     >  1:1009309/1‑150 (MQ=255)
             ttAGATAAATATGGCGATGCAAATATCAATCATAATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCg                                                                                                                                     <  1:1559332/150‑1 (MQ=255)
               aGATAAATATGGCGATGCAAATATCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGAAAATATGCTGACTGGTCGaa                                                                                                                                   >  2:1248637/1‑150 (MQ=255)
                gATAAATATGGCGATGCAAATATCAATCATTTTTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAAc                                                                                                                                  >  1:1148683/1‑150 (MQ=255)
                gATAAATATGGCGATGCAAATATCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACCGGTCGAAc                                                                                                                                  <  1:742017/150‑1 (MQ=255)
                 aTAAATATGGCGATGCAAATATCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACa                                                                                                                                 <  2:1511127/150‑1 (MQ=255)
                     atatGGCGATGCAAATATCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGt                                                                                                                             >  1:1277516/1‑150 (MQ=255)
                      tatGGCGATGCAAATATCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGt                                                                                                                             <  1:1381233/150‑1 (MQ=255)
                       atGGCGATGCAAATATCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGttt                                                                                                                           <  1:64131/150‑1 (MQ=255)
                          gCGATGCAAATATCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAACAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCt                                                                                                                        <  1:996560/150‑1 (MQ=255)
                          gCGATGCAAATATCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCt                                                                                                                        >  1:1564803/1‑150 (MQ=255)
                          gCGATGCAAATATCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCt                                                                                                                        <  1:1003050/150‑1 (MQ=255)
                           cGATGCAAATATCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTa                                                                                                                       <  2:1009309/150‑1 (MQ=255)
                            gATGCAAATATCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCAAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGAATGGTCTAACATAGTTTGCTaa                                                                                                                      >  2:706793/1‑150 (MQ=255)
                               gCAAATATCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACa                                                                                                                   <  2:1293527/150‑1 (MQ=255)
                               gCAAATATCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACa                                                                                                                   >  2:1399503/1‑150 (MQ=255)
                                cAAATATCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAg                                                                                                                  <  2:351637/150‑1 (MQ=255)
                                 aaaTATCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGt                                                                                                                 <  1:1450903/150‑1 (MQ=255)
                                     atcaatcaTTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGATAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGtcatc                                                                                                             <  2:308227/150‑1 (MQ=255)
                                       caatcaTTATTATAGAAACTTAGAAATAAAACACTAACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTAAATTAATTCGACCAGTCAGAAAATATGCTGACTTGTAGAACATAGTTTGATAAACAGtcctcaa                                                                                                           >  1:172991/1‑149 (MQ=255)
                                       aaatcaTTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCac                                                                                                           <  2:800548/149‑1 (MQ=255)
                                          tcaTTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCactga                                                                                                        <  2:698865/150‑1 (MQ=255)
                                            attattATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAg                                                                                                                              <  1:275146/126‑1 (MQ=255)
                                            attattATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAg                                                                                                                              >  2:1245797/1‑126 (MQ=255)
                                            attattATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAg                                                                                                                              >  2:275146/1‑126 (MQ=255)
                                            attattATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAg                                                                                                                              <  1:1245797/126‑1 (MQ=255)
                                                 tataGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATgt                                                                                                 >  1:750634/1‑150 (MQ=255)
                                                  ataGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATgta                                                                                                >  1:515082/1‑149 (MQ=255)
                                                  ataGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGAAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATgtc                                                                                                <  1:1248637/150‑1 (MQ=255)
                                                     gAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATgtcagt                                                                                             <  2:410867/150‑1 (MQ=255)
                                                     gAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATgtcagt                                                                                             >  1:1524470/1‑150 (MQ=255)
                                                     gAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATACGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATgtcagt                                                                                             >  2:1715695/1‑150 (MQ=255)
                                                         cTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGACatcagtcagt                                                                                         <  1:1219010/150‑3 (MQ=255)
                                                          ttAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCacac                                                                                        >  1:1173865/1‑150 (MQ=255)
                                                            aGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACTGTCATCACTGACTGATGGCAGTCccacca                                                                                      >  2:490006/1‑150 (MQ=255)
                                                                aTAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCg                                                                                  <  1:817604/150‑1 (MQ=255)
                                                                  aaaaCACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGcc                                                                                <  1:1399503/150‑1 (MQ=255)
                                                                        cTCACACCCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTAATCACTGACTGATGTCAGTCACAACATACGCCAGttaa                                                                          >  1:815394/1‑148 (MQ=255)
                                                                         tCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCaa                                                                         >  2:1047796/1‑150 (MQ=255)
                                                                          cacaACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAAt                                                                        >  1:938828/1‑150 (MQ=255)
                                                                           acaACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATc                                                                       <  2:1148683/150‑1 (MQ=255)
                                                                            caACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAATTATGCTGACTGGTCGAACATAGGTTGCTAAACAGTCATCACTGACTGATGTCAGGCACACCATCCGCCAGGTCAATcc                                                                      >  2:127202/1‑149 (MQ=255)
                                                                             aaCCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTTCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAg                                                                     >  1:231078/1‑150 (MQ=255)
                                                                              aCCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAgg                                                                    >  1:1605947/1‑150 (MQ=255)
                                                                               cccAAAATTAGAGCATCCGGCAAAAGAGTGGGTTAACATCATAGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTAGATAAACAGTCATCACTCACTGATGTCAGTCCCACCATCCGCCACTTCAATCAGGc                                                                   >  2:301227/1‑150 (MQ=255)
                                                                               cccAAAAATAGATCATCAGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGc                                                                   <  2:650483/150‑1 (MQ=255)
                                                                               cccAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATTTCAGTCACACCATCCGCCAGTTCAATCAGGc                                                                   >  1:101116/1‑150 (MQ=255)
                                                                               cccAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGc                                                                   <  2:1459562/150‑1 (MQ=255)
                                                                                  aaaaaTAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGaa                                                                >  2:964492/1‑150 (MQ=255)
                                                                                      aTAGAGCATCCTGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATaaa                                                            <  2:190257/150‑1 (MQ=255)
                                                                                      aTAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATaaa                                                            <  1:683423/150‑1 (MQ=255)
                                                                                          agCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTcc                                                        >  1:1172832/1‑150 (MQ=255)
                                                                                                     aaaaGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAAc                                              <  2:895630/150‑1 (MQ=255)
                                                                                                      aaaGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAAcc                                             >  1:460152/1‑150 (MQ=255)
                                                                                                          agtgagtTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCtgt                                         >  2:879463/1‑150 (MQ=255)
                                                                                                           gtgagtTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCtgtg                                        <  2:1564803/150‑1 (MQ=255)
                                                                                                               gtTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTAAATCAGGCGAATAAATTCCGCCATGTAACCCTGTGGaaa                                    >  2:223123/1‑148 (MQ=255)
                                                                                                                ttAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCTGTGGATCt                                   >  1:15485/1‑150 (MQ=255)
                                                                                                                 tAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATaa                                                             >  2:67913/1‑123 (MQ=255)
                                                                                                                 tAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATaa                                                             <  1:67913/123‑1 (MQ=255)
                                                                                                                 tAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCTGTGGATCtt                                  >  2:1630840/1‑150 (MQ=255)
                                                                                                                   aCATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCTGTGGATCTTcc                                <  2:1391748/150‑1 (MQ=255)
                                                                                                                    catcatTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCTGTGGATCTTccc                               >  1:1517481/1‑150 (MQ=255)
                                                                                                                          tGTTAATTAATTCGACCAGTCAGCAAATATGCTGGCTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCTGTGGATCTTCCCCTTTTg                         >  1:502091/1‑150 (MQ=255)
                                                                                                                           gTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCTGTGGATCTTCCCCTTTTGc                        >  2:384562/1‑150 (MQ=255)
                                                                                                                              aattaattCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCTGTGGATCTTCCCCTTTTGCctg                     >  1:930479/1‑150 (MQ=255)
                                                                                                                              aattaattCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCTGTGGATCTTCCCCTTTTGCctg                     <  2:1173865/150‑1 (MQ=255)
                                                                                                                                 taattCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCTGTGGATCTTCCCCTTTTGCctgctg                  >  2:336239/1‑150 (MQ=255)
                                                                                                                                 taattCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCTGTGGATCTTCCCCTTTTGCctgctg                  <  1:1338978/150‑1 (MQ=255)
                                                                                                                                   attCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACCCCATCCGCCAGTTCAATCAGGCGAATAAATTCGGCCCTGTTACCCTGGGGATCTGCCCCTTTTGCCTGCTGAg                >  2:1217834/1‑150 (MQ=255)
                                                                                                                                   attCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCTCCAGTTCAATAATGCGAATAAATTCCGCCCTGTAAACCTGTGGATCTTCACCTTTTGCCTGCTGAg                >  2:1211767/1‑150 (MQ=255)
                                                                                                                                    ttCGACCAGTCAGCATATTTGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCTGTGGATCTTCCCCTTTTGCCTGCTGAGc               >  2:1188165/1‑150 (MQ=255)
                                                                                                                                    ttCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATCCCGCCCTGTAACCCTGTGGATCTTCCCCTTTTGCCTGCTGAGc               <  1:1375438/150‑1 (MQ=255)
                                                                                                                                     tCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCTGTGGATCTTCCCCTTTTGCCTGCTGAGcc              <  2:1172832/150‑1 (MQ=255)
                                                                                                                                     tCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCTGTGGATCTTCCCCTTTTGCCTGCTGAGcc              >  1:1632875/1‑150 (MQ=255)
                                                                                                                                       gACCAGTCAGCATATTTGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGCCGAATAAATTCCGACCTGTAACCATGTCGATCTTCCCCTTTTTCCTGCTGAGACCa            >  2:1216620/1‑150 (MQ=255)
                                                                                                                                       gACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGCCACACCATCCGCCAGTTCAATCAGGCGAATAAATTACTCCCTGTAACCCTGTGGCTCTTCCCCTTTTGACTGCTGAGcccc            >  1:75707/1‑149 (MQ=255)
                                                                                                                                         ccAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCTGTGGATCTTCCCCTTTTGCCTGCTGAGCCCACt          >  2:1427321/1‑150 (MQ=255)
                                                                                                                                          cAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCTGTGGATCTTCCCCTTTTGCCTGCTGAGCCCACTg         <  1:964492/150‑1 (MQ=255)
                                                                                                                                           aGTCAGCATATTTGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCTGTGGATCTTCCCCTTTTGCCTGCTGAGCCCACTGt        >  1:1275276/1‑150 (MQ=255)
                                                                                                                                           aGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCTGTGGATCTTCCCCTTTTGCCTGCTGAGCCCACTGt        >  1:1640370/1‑150 (MQ=255)
                                                                                                                                           aGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCTGTGGATCTTCCCCTTTTGCCTGCTGAGCCCACTGt        >  2:1648764/1‑150 (MQ=255)
                                                                                                                                            gTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCTGTGGATCTTCCCCTTTTGCCTGCTGAGCCCACTGtt       <  2:1145304/150‑1 (MQ=255)
                                                                                                                                                 cAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCTGTGGATCTTCCCCTTTTGCCTGCTGAGCCCACTGTTTGATc  >  2:1103784/1‑150 (MQ=255)
                                                                                                                                                 cAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCTGTGGATCTTCCCCTTTTGCCTGCTGAGCCCACTGTTTGATc  <  2:16011/150‑1 (MQ=255)
                                                                                                                                                 cAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCTGTGGATCTTCCCCTTTTGCCTGCTGAGCCCACTGTTTGATc  >  2:1520246/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CAGAAATTGTTATTTAGATAAATATGGCGATGCAAATATCAATCATTATTATAGAAACTTAGAAATAAAACACTCACAACCCAAAAATAGAGCATCCGGCAAAAGAGTGAGTTAACATCATTGTTAATTAATTCGACCAGTCAGCAAATATGCTGACTGGTCGAACATAGTTTGCTAAACAGTCATCACTGACTGATGTCAGTCACACCATCCGCCAGTTCAATCAGGCGAATAAATTCCGCCCTGTAACCCTGTGGATCTTCCCCTTTTGCCTGCTGAGCCCACTGTTTGATC  >  NC_000913/2383810‑2384103

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: