Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,385,594 G→A 5.7% A43A (GCC→GCT yfbK ← Von Willebrand factor domain putative lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,385,5940GA 5.7% 333.5 / 5.8 104A43A (GCC→GCTyfbKVon Willebrand factor domain putative lipoprotein
Reads supporting (aligned to +/- strand):  ref base G (51/47);  new base A (4/2);  total (55/49)
Fisher's exact test for biased strand distribution p-value = 6.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.28e-02

Only 100 of 109 total aligned reads displayed.

TTTGCTTTAGCCGCTCTTGCAAATGTTGGCGCTTCCTGCAATCGCCCCTGTAAAGCCTGTTTGTCTGAATATTGTTGCACTTCTTGCTGGGCCAAGGCTTTCGCGGCGGCGGCGCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGCACTTGCTGCTCTGTGGGAGTACTGGGTTGTTGTTGCTGACTTTCCTTATTCTCAGGTTGCGGCCCACATCCTGACAAAATCAAACTACTCATAAGCAACATGATTATATTTTTATTTCGCATATAAAATCCTTTTAAATAAAC  >  NC_000913/2385445‑2385743
                                                                                                                                                     |                                                                                                                                                     
tttGCTTTAGCCGCTCTTGCAAATGTTGGCGCTTCCTGCAATCGCCCCTGTAAAGCCTGTTTGTCTGAATATTGTTGCACTTCTTGCTGGGCCAAGGCTTTCGCGGCGGCGGCGCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGcgcg                                                                                                                                                       <  1:1332195/150‑1 (MQ=255)
 ttGCTTTAGCCGCTCTTGCAAATGTTGGCGCTTCCTGCAATCGCCCCTGTAAAGCCTGTTTGTCTGAATATTGTTGCACTTCTTGCTGGGCCAAGGCTTTCGCGGCGGCGGCGCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCgg                                                                                                                                                      <  1:58568/150‑1 (MQ=255)
  tGCTTTAGCCGCTCTTGCAAATGTTGGCGCTTCCTGCAATCGCCCCTGTAAAGCCTGTTTGTCTGAATATTGTTGCACTTCTTGCTGGGCCAAGGCTTTCGCGGCGGCGGCGCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGc                                                                                                                                                     <  2:361491/150‑1 (MQ=255)
  tGCTTTAGCCGCTCTTGCAAATGTTGGCGCTTCCTGCAATCGCCCCTGTAAAGCCTGTTTGTCTGAATATTGTAGCACTTCTTGCTGGGCCAAGGCTTTCGCGGCGGCGGCGCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGc                                                                                                                                                     >  2:217172/1‑150 (MQ=255)
   gCTTTAGCCGCTCTTGCAAATGTTGGCGCTTCCTGCAATCGCCCCTGTAAAGCCTGTTTGTCTGAATATTGTTGCACTTCTTGCTGGGCCAAGGCTTTCGCGGCGGCGGCGCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCa                                                                                                                                                    <  2:964832/150‑1 (MQ=255)
   gCTTTAGCCGCTCTTGCAAATGTTGGCGCTTCCTGCAATCGCCCCTGTAAAGCCTGTTTGTCTGAATATTGTTGCACTTCTTGCTGGGCCAAGGCTTTCGCGGCGGCGGCGCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCa                                                                                                                                                    >  1:972117/1‑150 (MQ=255)
    cTTTAGCCGCTCTTGCAAATGTTGGCGCTTCCTGCAATCGCCCCTGTAAAGCCTGTTTGTCTGAATATTGTTGCACTTCTTGCTGGGCCAAGGCTTTCGCGGCGGCGGCGCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCaa                                                                                                                                                   >  1:25830/1‑150 (MQ=255)
     tttAGCCGCTCTTGCAAATGTTGGCGCTTCCTGCAATCGCCCCTGTAAAGCCTGTTTGTCTGAATATTGTTGCACTTCTTGCTGGGCCAAGGCTTTCGCGGCGGCGGCGCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAg                                                                                                                                                  <  1:560085/150‑1 (MQ=255)
        aGCCGCTCTTGCAAATGTTGGCGCTTCCTGCAATCGCCCCTGTAAAGCCTGTTTGTCTGAATATTGTTGCACTTCTTGCTGGGCCAAGGCTTTCGCGGCGGCGGCGCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCa                                                                                                                                                    >  1:1014840/1‑145 (MQ=255)
        aGCCGCTCTTGCAAATGTTGGCGCTTCCTGCAATCGCCCCTGTAAAGCCTGTTTGTCTGAATATTGTTGCACTTCTTGCTGGGCCAAGGCTTTCGCGGCGGCGGCGCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCa                                                                                                                                                    <  2:1014840/145‑1 (MQ=255)
        aGCCGCTCTTGCAAATGTTGGCGCTTCCTGCAATCGCCCCTGTAAAGCCTGTTTGTCTGAATATTGTTGCACTTCTTGCTGGGCCAAGGCTTTCGCGGCGGCGGCGCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGCAc                                                                                                                                               >  1:1193239/1‑150 (MQ=255)
        aGCCGCTCTTGCAAATGTTGGCGCTTCCTGCAATCGCCCCTGTAAAGCCTGTTTGTCTGAATATTGTTGCACTTCTTGCTGGGCCAAGGCTTTCGCGGCGGCGGCGCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGCAc                                                                                                                                               <  1:403944/150‑1 (MQ=255)
        aGCCGCTCTTGCAAATGTTGGCGCTTCCTGCAATCGCCCCTGTAAAGCCTGTTTGTCTGAATATTGTTGCACTTCTTGCTGGGCCAAGGCTTTCGCGGCGGCGGCGCTTTGCTCAGACTCTTTTATTGCAGCTTGCTGCGCGGCAAGCAc                                                                                                                                               >  1:174837/1‑150 (MQ=255)
         gCCGCTCTTGCAAATGTTGGCGCTTCCTGCAATCGCCCCTGTAAAGCCTGTTTGTGTGAATATTGTTGCACTTCTTGCTGGGCCAAGGCTTTCGCGGCGGCGGCGCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGCACt                                                                                                                                              >  1:1705599/1‑150 (MQ=255)
         gCCGCTCTTGCAAATGTTGGCGCTTCCTGCAATCGCCCCTGTAAAGCCTGTTTGTCTGAATATTGTTGCACTTCTTGCTGGGCCAAGGCTTTCGCGGCGGCGGCGCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGCACt                                                                                                                                              >  1:811845/1‑150 (MQ=255)
          ccGCTCTTGCAAATGTTGGCGCTTCCTGCAATCGCCCCTGTAAAGCCTGTTTGTCTGAATATTGTTGCACTTCTTGCTGGGCCAAGGCTTTCGCGGCGGCGGCGCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGCACtt                                                                                                                                             >  1:311922/1‑150 (MQ=255)
             ctctTGCAAATGTTGGCGCTTCCTGCAATCGCCCCTGTAAAGCCTGTTTGTCTGAATATTGTTGCACTTCTTGCTGGGCCAAGGCTTTCGCGGCGGCGGCGCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGCACTtgct                                                                                                                                          >  2:805839/1‑150 (MQ=255)
                 tGCAAATGTTGGCGCTTCCTGCAATCGCCCCTGTAAAGCCTGTTTGTCTGAATATTGTTGCACTTCTTGCTGGGCCAAGGCTTTCGCGGCGGCGGCGCTTTGCTCAGCCTCTTTTATTGCAGCTTGCCGCGCAGCAAGctgc                                                                                                                                              >  2:38342/1‑139 (MQ=255)
                 tGCAAATGTTGGCGCTTCCTGCAATCGCCCCTGTAAAGCCTGTTTGTCTGAATATTGTTGCACTTCTTGCTGGGCCAAGGCTTTCGCGGCGGCGGCGCTTTGCTCAGCCTCTTTTATTGCAGCTTGCCGCGCAGCAAGctgc                                                                                                                                              <  1:78822/142‑4 (MQ=255)
                 tGCAAATGTTGGCGCTTCCTGCAATCGCCCCTGTAAAGCCTGTTTGTCTGAATATTGTTGCACTTCTTGCTGGGCCAAGGCTTTCGCGGCGGCGGCGCTTTGCTCAGCCTCTTTTATTGCAGCTTGCCGCGCAGCAAGctgc                                                                                                                                              >  2:78822/1‑139 (MQ=255)
                 tGCAAATGTTGGCGCTTCCTGCAATCGCCCCTGTAAAGCCTGTTTGTCTGAATATTGTTGCACTTCTTGCTGGGCCAAGGCTTTCGCGGCGGCGGCGCTTTGCTCAGCCTCTTTTATTGCAGCTTGCCGCGCAGCAAGctgc                                                                                                                                              <  1:38342/142‑4 (MQ=255)
                  gCAAATGTTGGCGCTTCCTGCAATCGCCCCTGTAAAGCGTGTTTGTCTGAATATTGTTGCACTTCTTGCTGGGCCACGGCTTTCGCGGCGGCGGCGCTTTGCTCAGCCTCGTTTATTGCAGCTTGATGCGCGGCAAGCACTTGCTCctct                                                                                                                                     >  2:724302/1‑150 (MQ=255)
                     aaTGTTGGCGCTTCCTGCAATCGCCCCTGTAACGCCTGTTTGTCTGAATATTGTGGCACTTCTTGCTGGGCCAAGGCTTTCGCGGCGGCGGCGCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGCACTTGCTGCTCtgtg                                                                                                                                  <  1:1201994/150‑1 (MQ=255)
                     aaTGTTGGCGCTTCCTGCAATCGCCCCTGTAAAGCCTGTTTGTCTGAATATTGTTGCACTTCTTGCTGGGCCAAGGCTTTCGCGGCGGCGGCGCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGCACTTGCTGCTCtgtg                                                                                                                                  <  1:1208120/150‑1 (MQ=255)
                      aTGTTGGCGCTTCCTGCAATCGCCCCTGTAAAGCCTGTGTGTCTGAATATTGTTGCACTTCTTGCTGGGCCAAGGCTTTCGCGGCGGCGGCGCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGCACTTGCTGCTCTGTgg                                                                                                                                 <  1:1437840/150‑1 (MQ=255)
                       tGTTGGCGCTTCCTGCAATCGCCCCTGTAAAGCCTGTTTGTCTGAATATTGTTGCACTTCTTGCTGGGCCAAGGCTTTCGCGGCGGCGGCGCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGCACTTGCTGCTCTGTggg                                                                                                                                >  1:1120747/1‑150 (MQ=255)
                           ggCGCTTCCTGCAATCGCCCCTGTAAAGCCTGTTTGTCTGAATATTGTTGCACTTCTTGCTGGGCCAAGGCTTTCGCGGCGGCGGCGCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCACGCACTTGCTGCTCTGTGGGAGTa                                                                                                                            >  1:495996/1‑150 (MQ=255)
                            gcgcTTCCTGCAATCGCCCCTGTAAAGCCTGTTTGTCTGAATATTGTTGCACTTCTTGCTGGGCCAAGGCTTTCGCGGCGGCGGCGCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGCACTTGCTGCTCTGTGGGAGTAc                                                                                                                           >  1:432331/1‑150 (MQ=255)
                              gcTTCCTGCAATCGCCCCTGTAACGCCTGTTTGTCTGAATATTGTTGCACTTCTTGCTGGGCCAAGGCTTTCGCGGCGGCGGCGCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGCACTTGCTGCTCTGTGGGAGTACTg                                                                                                                         >  1:1278481/1‑150 (MQ=255)
                              gcTTCCTGCAATCGCCCCTGTAAAGCCTGTTTGTCTGAATATTGTTGCACTTCTTGCTGGGCCAAGGCTTTCGCGGCGGCGGCGCATTGCTCAGCATCTATTATTGCAGCTTTCTGCGCGGCAAGACCTTCCTGCTCTGTGGAAGTACTg                                                                                                                         >  2:1313315/1‑150 (MQ=255)
                               cgtCCTGCACTCGCCCCTTTCCAGCCTGGTTGTCCGACTATTGTTGCACTGCTTGCTGGGCCAAGGCTTTCGCGGCGGCGGCGCTTTTCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGCACTTGCTGCTCTGTGGGAGGACTgg                                                                                                                        <  2:252437/148‑1 (MQ=255)
                               cTTCCTGCAATCGCCCCTGTAAAGCCTGTTTGTCTGAATATTGTTGCACTTCTTGCTGGGCCAAGGCTTTCGCGGCGGCGGCGCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGCACTTGCTGCTCTGTGGGAGTACTgg                                                                                                                        >  2:323272/1‑150 (MQ=255)
                                ttCCTGCAATCGCCCCTGTAAAGCCTGTTTGTCTGAATATTGTTGCACTTCTTGCTGGGCCAAGGCTTTCGCGGCGGCGGCGCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGCACTTGCTGCTCTGTGGGAGTACTggg                                                                                                                       <  1:1334674/150‑1 (MQ=255)
                                ttCCTGCAATCGCCCCTGTAAAGCCTGTTTGTCTGAATATTGTTGCACTTCTTGCTGGGCCAAGGCTTTCGCGGCGGCGGCGCTTTGCGCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGCACTTGCTGCTCTGTGGGAGTACTggg                                                                                                                       >  2:1469139/1‑150 (MQ=255)
                                 tCCTGCAATCGCCCCTGTAAAGCCTGTTTGTCTGAATATTGTTGCACTTCTTGCTGGGCCAAGGCTTTCGCGGCGGCGGCGCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGCACTTGCTGCTCTGTGGGAGTACTGGgt                                                                                                                      <  1:1027875/150‑1 (MQ=255)
                                  ccTGCAATCGCCCCTGTAAAGCCTGTTTGTCTGAATATTGTTGCACTTCTTGCTGGGCCAAGGCTTTCGCGGCGGCGGCGCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGCACTTGCTGCTCTGTGGGAGTACTGGgtt                                                                                                                     >  1:824219/1‑150 (MQ=255)
                                   cTGCAATCGCCCCTGTAAAGCCTGTTTGTCTGAATATTGTTGCACTTCTTGCTGGGCCAAGGCTTTCGCGGCGGCGGCGCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGCACTTGCTGCTCTGTGGGAGTACTGGgttg                                                                                                                    <  2:432331/150‑1 (MQ=255)
                                   cTGCAATCGCCCCTGTAAAGCCTGTTTGTCTGAATATTGTTGCACTTCTTGCTGGGCCAAGGCTTTCGCGGCGGCGGCGCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGCACTTGCTGCTCTGTGGGAGTACTGGgttg                                                                                                                    >  1:1136578/1‑150 (MQ=255)
                                    tGCAATCGCCCCTGTAAAGCCTGTTTGTCTGAATATTGTTGCACTTCTTGCTGGGCCAAGGCTTTCGCGGCGGCGGCGCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGCACTTGCTGCTCTGTGGGAGTACTGGgttgt                                                                                                                   <  1:1474744/150‑1 (MQ=255)
                                     gCAATCGCCCCTGTAAAGCCTGTTTGTCTGAATATTGTTGCACTTCTTGCTGGGCCAAGGCTTTCGCGGCGGCGGCGCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGCACTTGCTGCTCTGTGGGAGTACTGGgttgtt                                                                                                                  >  1:547294/1‑150 (MQ=255)
                                       aaTCGCCCCTGTAAAGCCTGTTTGTCTGAATATTGTTGCACTTCTTGCTGGGCCAAGGCTTTCGCGGCGGCGGCGCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGCACTTGCTGCTCTGTGGGAGTACTGGgttgttgt                                                                                                                >  2:1277654/1‑150 (MQ=255)
                                       aaTCGCCCCTGTAAAGCCGGGTTGTCTGAATATTGTTGCACTTCTTGCTGGGCCAAGGCTTTCGCGGCGGCGGCGCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGCACTTGCTGCTCTGTGGGAGTACTGGgttgttgt                                                                                                                <  1:840447/150‑1 (MQ=255)
                                          cGCCCCTGTAAAGCCTGTTTGTCTGAATATTGTTGCACTTCTTGCTGGGCCAAGGCTTTCGCGGCGGCGGCGCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGCACTTGCTGCTCTGTGGGAGTACTGGGTTGTTGTTGc                                                                                                             <  2:1481865/150‑1 (MQ=255)
                                           gCCCCTGTAAAGCCTGTTTGGCTGAAGATTGTTGCACTTCTTGCTGGGCCAAGGCTTTCGCGGCGGCGGCGCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGCACTTGCTGCTCTGTGGGAGTACTGGGTTGTTGTTGCt                                                                                                            <  2:321663/150‑1 (MQ=255)
                                             acatGTAAAGCCTGTTTGTCTGAATATTGATGCACTTCTTGCTGGGCCAAGGCTTTCGCGGCGGCGGCGCTTTGCTCAGCATCTTTTATTGCAGCTTGCTGCGCGGCAAGCACTTGCTGCTCTGTGGGATTACTGGGTTGTTGTTGCTGa                                                                                                          >  1:1069115/4‑150 (MQ=255)
                                              ccTGTAAAGCCTGTTTGTCTGAATATTGTTGCACTTCTTGCTGGGCCAAGGCTTTCGCGGCGGCGGCGCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGCACTTGCTGCTCTGTGGGAGTACTGGGTTGTTGTTGCTGAc                                                                                                         >  2:1361922/1‑150 (MQ=255)
                                                  tAAAGCCTGTTTGTCTGATTATTGTTGCACTTCTTGCTGGGCCAAGGCTTTCGCGGCGGCGGCGCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGCACTTGCTGCTCTGTGGGAGTACTGGGTTGTTGTTGCTGACTTTc                                                                                                     >  1:288086/1‑150 (MQ=255)
                                                   aaaGCCTGTTTGTCTGAATATTGTTGCACTTCTTGCTGGGCCAAGGCTTTCGCGGCGGCGGCGCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGCACTTGCTGCTCTGTGGGAGTACTGGGTTGTTGTTGCTGACTTTcc                                                                                                    <  1:1361922/150‑1 (MQ=255)
                                                      gCCTGTTTGTCCGAATATTGTTGCACTTCTTGCTGGGCCAAGGCTTTCGCGGCGGCGGCGCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGCACTTGCTGCTCTGTGGGAGTACTGGGTTGTTGTTGCTGACTTTCCTTa                                                                                                 <  1:1298761/150‑1 (MQ=255)
                                                         tGTTTGTCTGAATATTGTTGCACTTCTTGCTGGGCCAAGGCTTTCGCGGCGGCGGCGCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGCACTTGCTGCTCTGTGGGAGTACTGGGTTGTTGTTGCTGACTTTCCTTATtc                                                                                              <  2:25830/150‑1 (MQ=255)
                                                          gTTTGTCTGAATTTTGTTGCTCTTCTTGGTGGGCCAAGGCTTTCGCGGCGGCGGCGCTGTGCTCAGCCTCTTTTATTGCAGCTTGTTGCGCGGCAAGCACTTGCTGCTCTGTGGGAGTACTGGGTTGTTGTTGCTGACTTTCCTTATtct                                                                                             >  2:380409/1‑150 (MQ=255)
                                                            ttGTCTGAATATTGTTGCACTTCTTGCTGGGCCAAGGCTTTCGCGGCGGCGGCGCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGCACTTGCTGCTCTGTGGGAGTACTGGGTTGTTGTTGCTGACTTTCCTTATTCTCa                                                                                           <  1:1277654/150‑1 (MQ=255)
                                                              gTCTGAATATTGTTGCACTTCTTGCTGGGCCAAGGCTTTCGCGGCGGCGGCGCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGCACTTGCTGCTCTGTGGGAGTACTGGGttgttgttg                                                                                                              >  1:1078505/1‑129 (MQ=255)
                                                              gTCTGAATATTGTTGCACTTCTTGCTGGGCCAAGGCTTTCGCGGCGGCGGCGCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGCACTTGCTGCTCTGTGGGAGTACTGGGttgttgttg                                                                                                              <  2:1078505/129‑1 (MQ=255)
                                                              gTCTGAATATTGTTGCACTTCTTGCTGGGCCAAGGCTTTCGCGGCGGCGGCGCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGCACTTGCTGCTCTGTGGGAGTACTGGGTTGTTGTTGCTGACTTTCCTTATTCTCAgg                                                                                         <  1:959225/150‑1 (MQ=255)
                                                                cTGAATATTGTTGCACTTCTTGCTGGGCCAAGGCTTTCGCGGCGGCGGCGCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGCACTTGCTGCTCTGTGGGAGTACTGGGTTGTTGTTGCTGACTTTCCTTATTCTCAGGtt                                                                                       >  1:459302/1‑150 (MQ=255)
                                                                  gAATATTGTTGCACTTCTTGCTGGGCCAAGGCTTTCGCGGCGGCGGCGCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGCACTTGCTGCTCTGTGGGAGTACTGGGTTGTTGTTGCTGACTTTCCTTATTCTCAGGTTGc                                                                                     >  2:870402/1‑150 (MQ=255)
                                                                    atatTGTTGCACTTCTTGCTGGGCCAAGGCTTTCGCGGCGGCGGCGCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGCACTTGCTGCTCTGTGGGAGTACTGGGTTGTTGTTGCTGACTTTCCTTATTCTCAGGTTGCgg                                                                                   <  1:1379628/150‑1 (MQ=255)
                                                                         gttgCACTTCTTGCTGGGCCAAGGCTTTCGCGGCGGCGGCGCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGCACTTGCTGCTCTGTGGGAGTACTGGGTTGTTGTTGCTGACTTTCCTTATTCTCAGGTTGCGGCCcac                                                                              >  1:1282455/1‑150 (MQ=255)
                                                                           tgCACTTCTTGCTGGGCCAAGGCTTTCGCGGCGGCGGCGCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGCACTTGCTGCTCTGTGGGAGTACTGGGTTGTTGTTGCTGACTTTCCTTATTCTCAGGTTGCGGCCCACAt                                                                            >  2:1361526/1‑150 (MQ=255)
                                                                             cACTTCTTGCTGGGCCAAGGCTTTCGCGGCGGCGGCGCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGCACTTGCTGCTCTGTGGGAGTACTGGGTTGTTGTTGCTGACTTTCCTTATTCTCAGGTTGCGGCCCACATcc                                                                          >  2:1037519/1‑150 (MQ=255)
                                                                              aCTTCTTGCTGGGCCAAGGCTTTCGCGGCGGCGGCGCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGCACTTGCTGCTCTGTGGGAGTACTGGGTTGTTGTTGCTGACTTTCCTTATTCTCAGGTTGCGGCCCACATCCt                                                                         <  2:1419903/150‑1 (MQ=255)
                                                                               cttcttGCTGGGCCAAGGCTTTCGCTGCGGCGGCGCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGCACTTGCTGCTCTGGGGGAGTACTGGGTTGTTGTTGCTGACTTTCCTTATTCTCAGGTTGCGGCCCACATCCTg                                                                        <  1:217172/150‑1 (MQ=255)
                                                                                ttcttGCTGGGCCAAGGCTTTCGCGGCGGCGGCGCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGCACTTGCTGCTCTGTGGGAGTACTGGGTTGTTGTTGCTGACTTTCCTTATTCTCAGGTTGCGGCCCACATCCTGa                                                                       <  2:1279756/150‑1 (MQ=255)
                                                                                ttcttGCTGGGCCAAGGCTTTCGCGGCGGCGGCGCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGCACTTGCTGCTCTGTGGGAGTACTGGGTTGTTGTTGCTGACTTTCCTTATTCTCAGGTTGCGGCCCACATCCTGa                                                                       <  1:1354210/150‑1 (MQ=255)
                                                                                  cttGCTGGGCCAAGGCGTTCGCGGCGGCGGCGCTGTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGCACTTGCGGCTCTGTGGGAGTACTGGGTTGTTGTTGCTGACTTTCCTTATTCTCAGGGTGCGGCCCACATCCTGACa                                                                     <  2:832168/150‑1 (MQ=255)
                                                                                  cttGCCGGGCCAAGGCTTTCGCGGCGGCGGCGCTTTGCTCAGCCGCTTGTATTGCAGCTGGCTGCGCGGCAATCACTTGCTGCGCTGTGGGAGTACTGGGGTGTTGTTGCTGACTTTCCTTATTCTCAGGTTGCGGCCCACATCCTGACa                                                                     <  2:832161/150‑1 (MQ=255)
                                                                                    tGCTGGGCCAAGGCTTTCGCGGCGGCGGCGCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGCACTTGCTGCTCTGTGGGAGTACTGGGTTGTTGTTGCTGACTTTCCTTATTCTCAGGTTGCGGCCCACATCCTGACaaa                                                                   >  1:892680/1‑150 (MQ=255)
                                                                                    tGCTGGGCCAAGGCTTTCGCGGCGGCGGCGCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGCACTTGCTGCTCTGTGGGAGTACTGGGTTGTTGTTGCTGACTTTCCTTATTCTCAGGTTGCGGCCCACATCCTGACaaa                                                                   >  2:523333/1‑150 (MQ=255)
                                                                                    tGCTGGGCCAAGGCTTTCGCGGCGGCGGCGCTTTGCTCAGACTCTTTTATTGCAGCTTGCTGCGCGGCAAGCACTTGCTGCTCTGTGGGAGTACTGGGTTGTTGTTGCTGACTTTCCTTATTCTCAGGTTGCGGCCCACATCCTGACaaa                                                                   >  2:583709/1‑150 (MQ=255)
                                                                                        gggCCAAGGCTTTCGCGGCGGCGGCGCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGCACTTGCTGCTCTGTGGGAGTACTGGGTTGTTGTTGCTGACTTTCCTTATTCTCAGGTTGCGGCCCACATCCTGACAAAATCa                                                               <  2:1136578/150‑1 (MQ=255)
                                                                                                    tCGCGGCGGCGGCGCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGCACTTGCTGCTCTGTGGGAGTAATGTGTTGTTGTTGCTGACTTTCCTTATTCTCAGGTTGCGGCCCACATCCTGACAAAATGTTACTAGTCATaa                                                   <  2:1231785/150‑1 (MQ=255)
                                                                                                      gcggcggcggcgCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGCACTTGCTGCTCTGTGGGAGTACTGGGTTGTTGTTGCTGACTTTCCTTATTCTCAGGTTGCGGCCCACATCCTGACAAAATCAAACTACTCATAAGc                                                 >  1:707896/1‑150 (MQ=255)
                                                                                                        ggcggcggcgCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGCACTTGCTGCTCTGTGGGAGTACTGGGTTGTTGTTGCTGACTTTCCTTATTCTCAGGTTGCGGCCCACATCCTGACAAAATCAAACTACTCATAAGCaa                                               <  2:811845/150‑1 (MQ=255)
                                                                                                         gcggcggcgCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGCACTTGCTGCTCTGTGGGAGTACTGGGTTGTTGTTGCTGACTTTCCTTATTCTCAGGTTGCGGCCCACATCCTGACAAAATCAAACTACTCATAAGCAAc                                              <  2:1705599/150‑1 (MQ=255)
                                                                                                            gcggcgCCTCGCTCCGCCTCATTTCTTGCAGCCTGCTGCGCGGCAAGCACTTGCTGCTCTGTGGGAGTACTGGGTTGTTGTTGCTGACTTTCCTAATTCTCAGGTTGCGGCCCACATCCTGACAAAATCAAACTACTCATAAGCAACATg                                           <  2:1120747/150‑1 (MQ=255)
                                                                                                             cggcgCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGCACTTGCTGCTCTGTGGGAGTACTGGGTTGTTGTTGCTGACTTTCCTTATTCTCAGGTTGCGGCCCACATCCTGACAAAATCAAACTACTCATAAGCAACATGa                                          >  1:495140/1‑150 (MQ=255)
                                                                                                               gcgcTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGCACTTGCTGCTCTGTGGGAGTACTGGGTTGTTGTTGCTGACTTTCCTTATTCTCAGGTTGCGGCCCACATCCTGACAAAATCAAACTACTCATAAGCAACATGAtt                                        <  2:1078654/150‑1 (MQ=255)
                                                                                                                       cTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGCACTTGCTGCTCTGTGGGAGTACTGGGTTGTTGTTGCTGACTTTCCTTATTCTCAGGTTGCGGCCCCCATCCGGACCAAATCAAACTACTCATAAGCAACATGATTATAttttt                                >  1:1579502/1‑150 (MQ=255)
                                                                                                                         cAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGCACTTGCTGCTCTGTGGGAGTACTGGGTTGTTGTTGCTGACTTTCCTTATTCTCAGGTTGCGGCCCACATCCTGACAAAATCAAACTACTCATAAGCAACATGATTATATTTTTAt                              <  2:1249255/150‑1 (MQ=255)
                                                                                                                          aGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGCACTTGCTGCTCTGTGGGAGTACTGGGTTGTTGTTGCTGACTTTCCTTATTCTCAGGTTGCGGCCCACATCCTGACAAAATCAAACTACTCATAAGCAACATGATTATATTTTTAtt                             <  1:870402/150‑1 (MQ=255)
                                                                                                                          aGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGCACTTGCTGCTCTGTGGGAGTACTGGGTTGTTGTTGCTGACTTTCCTTATTCTCAGGTTGCGGCCCACATCCTGACAAAATCAAACTACTCATAAGCAACATGATTATATTTTTAtt                             >  1:1177675/1‑150 (MQ=255)
                                                                                                                          aGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGCACTTGCTGCTCTGTGGGAGTACTGGGTTGTTGTTGCTGACTTTCCTTATTCTCAGGTTGCGGCCCACATCCTGACAAAATCAAACTACTCATAAGCAACATGATTATATTTTTAtt                             <  1:323272/150‑1 (MQ=255)
                                                                                                                           gCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGCACTTGCTGCTCTGTGGGAGTACTGGGTTGTTGTTGCTGACTTTCCTTATTCTCAGGTTGCGGCCCCCATCCTGACAAAATCAAACGACTCATAAGCAACATGATTATATTTTTAttg                            >  1:224871/1‑149 (MQ=255)
                                                                                                                             ctctTTTATTGCAGCTTGCTGCGCGGCAAGCACTTGCTGCTTGGTGGGAGTACTGGGTTGTTGTTGCTGACTTTCCTTATTCTCAGGTTGCGGCCCACCTCCTGACAAAATCAAACTACTCATAAGCAACATGATTATATTTTTATTTCg                          >  1:1348417/1‑150 (MQ=255)
                                                                                                                                ttttATTGCAGCTTGCTGCGCGGCAAGCACTTGCTGCTCTGTGGGAGTACTGGGTTGTTGTTGCTGACTTTCCTTATTCTCAGGTTGCGGCCCACATCCTGACAAAATCAAACTACTCATAAGCAACATGATTATATTTTTATTTCGCat                       >  1:78337/1‑150 (MQ=255)
                                                                                                                                   tATTGCAGCTTGCTGCGCGGCAAGCACTTGCTGCTCTGTGGGAGTACTGGGTTGTTGTTGCTGACTTTCCTTATTCTCAGGTTGCGGCCCACATCCTGACAAAATCAAACTACTCATAAGCAACATGATTATATTTTTATTTCGCAtata                    <  1:600808/150‑1 (MQ=255)
                                                                                                                                    gcaagtgCTTGCCGCGCAGCAAGCACTTGCTGCTCTGTGGGAGTACTGGGTTGTTGTTGCTGACTTTCCTTATTCTCAGGTTGCGGCCCACATCCTGACAAAATCAAACTACTCATAAGCAACATGATTATATTTTTATTTCGCATATaa                   >  1:1142660/7‑150 (MQ=255)
                                                                                                                                    aTTGCAGCTTGCTGCGCGGCAAGCACTTGCTGCTCTGTGGGAGTACTGGGTTGTTGTTGCTGACTTTCCTTATTCTCAGGTTGCGGCCCACATCCTGACAAAATCAAACTACTCATAAGCAACATGATTATATTTTTATTTCGCATATaa                   >  1:1090503/1‑150 (MQ=255)
                                                                                                                                     caagtgCTTGCCGCGCAGCAAGCACTTGCTGCTCTGTGGGAGTACTGGGTTGTTGTTGCTGACTTTCCTTATTCTCAGGTTGCGGCCCACATCCTGACAAAATCAAACTACTCATAAGCAACATGATTATATTTTTATTTCGCATATaaa                  >  2:1351009/6‑150 (MQ=255)
                                                                                                                                      tGCAGCTTGCTGCGCGGCAAGCACTTGCTGCTCTGTGGGAGTACTGGGTTGTTGTTGCTGACTTTCCTTATTCTCAGGTTGCGGCCCACATCCTGACAAAATCAAACTACTCATAAGCAACATGATTATATTTTTATTTCGCATATaaaa                 <  2:459248/150‑1 (MQ=255)
                                                                                                                                      ccgatcTTGCTGCGCGGCAAGCACTTTCTGCTCTTTGGGAGTACTTGGTTTTTGTTGCTGACTTTCCTTATTCTCAGGTTGCGGCCCACATCCTGACAAAATCTAACTACTCATAAGCAACATGATTATATTTTTATTTCGCATATaaat                 <  1:808377/145‑2 (MQ=255)
                                                                                                                                       gCAGCTTGCTGCGCGGCAAGCACTTGCTGCTCTGTGGGAGTACTGGGTTGTTGTTGCTGACTTTCCTTATTCTCAGGTTGCGGCCCACATCCTGACAAAATCAAACTACTCATAAGCAACATGATTATATTTTTATTTCGCATATAAAAt                >  2:772968/1‑150 (MQ=255)
                                                                                                                                       gCAGCTTGCTGCGCGGCAAGCACTTGCTGCTCTGTGGGAGTACTGGGTTGTTGTTGCTGACTTTCCTTATTCTCAGGTTGCGGCCCACATCCTGACAAAATCAAACTACTCATAAGCAACATGATTATATTTTTATTTCGCATATAAAAt                >  1:905271/1‑150 (MQ=255)
                                                                                                                                             tgctgcGCGGCAAGCACTTGCTGCTCTGTGGGAGTACTGTGTTGTTGTTGCTGACTTTCCTTATTCTCAGGTTGCGGCCCACATCCTGACAAAATCAAACTACTCATAAGCAACATGATTATATTTTTATTTCGCATATAAAATCCtttt          <  1:1037519/150‑1 (MQ=255)
                                                                                                                                             tgctgcGCGGCAAGCACTTGCTGCTCTGTGGGAGTACTGGGTTGTTGTGGCTGACTTTCCTTATTCTCAGGTTGCGGCCCACATCCTGACAAAATCAAACTACTCATAAGCAACATGATTATATTTTTATTTCGCATATAAAATCCtttt          <  2:992444/150‑1 (MQ=255)
                                                                                                                                             tgctgcGCGGCAAGCACTTGCTGCTCTGTGGGAGTACTCGGTTGTTGTTGCTGACTTTCCTTATTCTCAGGTTGCGGCCCACATCCTGACAAAATCAAAATACTCATAAGCAACATGATTATATTTTTATATCGCATATaaaaagatcgg          >  2:808377/1‑143 (MQ=255)
                                                                                                                                               ctgcGCGGCAAGCACTTGCTGCTCTGTGGGAGTACTGGGTTGTTGTTGCTGACTTTCCTTATTCTCAGGTTGCGGCCCACATCCTGACAAAATCAAACTACTCATAAGCAACATGATTATATTTTTATTTCGCATATAAAATCCTTTtaa        >  2:648680/1‑150 (MQ=255)
                                                                                                                                                  cgcgGCAAGCACTTGCTGCTCTGTGGGAGTACTGGGTTGTTGTTGCTGACTTTCCTTATTCTCAGGTTGCGGCCCACATCCTGACAAAATCAAACTACTCATAAGCAACATGATTATATTTTTATTTCGCATATAAAATCCTTTtaaata     <  2:1193239/150‑1 (MQ=255)
                                                                                                                                                     ggCAAGCACTTGCTGCTCTGTGGGAGTACTGGGTTGTTGTTGCTGACTTTCCTTATTCTCAGGTTGCGGCCCACATCCTGACAAAATCAAACTACTCATAAGCAACATGATTATATTTTTATTTCGCATATAAAATCCTTTTAAATAAAc  <  2:824219/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TTTGCTTTAGCCGCTCTTGCAAATGTTGGCGCTTCCTGCAATCGCCCCTGTAAAGCCTGTTTGTCTGAATATTGTTGCACTTCTTGCTGGGCCAAGGCTTTCGCGGCGGCGGCGCTTTGCTCAGCCTCTTTTATTGCAGCTTGCTGCGCGGCAAGCACTTGCTGCTCTGTGGGAGTACTGGGTTGTTGTTGCTGACTTTCCTTATTCTCAGGTTGCGGCCCACATCCTGACAAAATCAAACTACTCATAAGCAACATGATTATATTTTTATTTCGCATATAAAATCCTTTTAAATAAAC  >  NC_000913/2385445‑2385743

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: