Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,392,500 C→G 5.8% S181T (AGT→ACT)  nuoM ← NADH:ubiquinone oxidoreductase, membrane subunit M

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,392,5000CG 5.8% 343.2 / 7.5 101S181T (AGT→ACT) nuoMNADH:ubiquinone oxidoreductase, membrane subunit M
Reads supporting (aligned to +/- strand):  ref base C (45/50);  new base G (3/3);  total (48/53)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.38e-01

Only 100 of 102 total aligned reads displayed.

CGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCACCAGCATCATTTCCCAGAAGAAGAAGAACAGGAACATGTCGATGGCAAG  >  NC_000913/2392354‑2392648
                                                                                                                                                  |                                                                                                                                                    
cGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGCCCAcacc                                                                                                                                                   >  2:1661221/1‑147 (MQ=255)
  aTGAAGAAGCCCAGCATTAAATCGTAATCCAAACAACATGACATTAGCGTATTCATCAGATATTCATAGTTGATGTTACAGACGCCGGTGGCAAAGTAGATATAAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCAGTCGcc                                                                                                                                                 >  2:217402/1‑150 (MQ=255)
    gaagaaGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTg                                                                                                                                               <  2:564349/150‑1 (MQ=255)
      agaagCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTggg                                                                                                                                             >  2:1525424/1‑150 (MQ=255)
       gaagCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGgt                                                                                                                                            >  2:622002/1‑150 (MQ=255)
        aagCCCAGCATTAACAGGTATTTCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCGAGTGGCCTggtga                                                                                                                                           <  2:1420079/150‑4 (MQ=255)
         agCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGgtgt                                                                                                                                          >  2:510257/1‑150 (MQ=255)
          gCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCGAGTGGCCTggtgatg                                                                                                                                         <  2:588846/150‑6 (MQ=255)
          gCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTa                                                                                                                                         >  1:1215832/1‑150 (MQ=255)
           cccAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCGAGTGGCCTggtgatgt                                                                                                                                        <  1:721409/150‑7 (MQ=255)
            ccAGCATTAACAGGTCTTGCACACCACTGGACATTGGCGTATTCAGCCGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTaaa                                                                                                                                       <  2:162/150‑1 (MQ=255)
            ccAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTaaa                                                                                                                                       <  2:1181018/150‑1 (MQ=255)
            ccAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTaaa                                                                                                                                       <  1:1535636/150‑1 (MQ=255)
            ccAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCAAGACGCCGGTCGCATTTTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGGaaa                                                                                                                                       >  2:340821/1‑150 (MQ=255)
             cAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCATCATAGTTGAAGGTCCAGACGCCGGTCGCATAGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCATGGGTGTAAAt                                                                                                                                      >  1:1364443/1‑150 (MQ=255)
                cATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATgaa                                                                                                                                   <  2:95829/150‑1 (MQ=255)
                cATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATAGTTGAAGATCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATgaa                                                                                                                                   >  1:196906/1‑150 (MQ=255)
                  tttaCAGGTATTCCACACCACTGTACTTTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTGGAGCCGCCGGTCGCATTGTAGTGAACAATAACCAGCGCCAGGTTTGCAATCAACATCACCCGGCCACTCGCCTGGGTGTAATTtaaga                                                                                                                                 <  2:252215/147‑1 (MQ=255)
                  ttAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATgaaga                                                                                                                                 <  1:741019/150‑1 (MQ=255)
                       aGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTg                                                                                                                            >  1:1071994/1‑150 (MQ=255)
                        ggTATTCCACGCCACTGGACATTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTgg                                                                                                                           <  2:1625535/150‑1 (MQ=255)
                         gTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATgaaga                                                                                                                                 <  2:611325/143‑1 (MQ=255)
                         gTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATgaaga                                                                                                                                 >  1:611325/1‑143 (MQ=255)
                           aTTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTg                                                                                                                        <  1:467352/150‑1 (MQ=255)
                             tCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTgcc                                                                                                                      <  2:562051/150‑1 (MQ=255)
                               cacaCCACTGGACATTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTgccgc                                                                                                                    <  2:1381816/150‑1 (MQ=255)
                                 caccacTGGACATTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGccgccg                                                                                                                  <  2:1508365/150‑1 (MQ=255)
                                 caccacTGGACATTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGccgccg                                                                                                                  >  1:1526952/1‑150 (MQ=255)
                                 caccacTGGACATTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGccgccg                                                                                                                  <  2:490783/150‑1 (MQ=255)
                                   ccacTGGACATTGGCGTATTCAGCAGCTCTGCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTg                                                                                                                <  2:1710139/150‑1 (MQ=255)
                                     acTGGACATTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGAt                                                                                                              >  1:367711/1‑150 (MQ=255)
                                           cATTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGt                                                                                                        >  1:427117/1‑150 (MQ=255)
                                           cATTGGCGTAATCAGCAGCTCTTCATAGGTGATGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAACCCCGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATAcgcgg                                                                                                        <  1:1355389/150‑2 (MQ=255)
                                               ggCGTATTCAGCAGCTCTTCATAGTTGAAGGGCCACACGCCGGTCGCATTGTAGTGAACAAATACCAGCGCCAGGATGGCAATCAACATCACCAGTCCACTCGCCTGTGTGTAAATGAAGAACTTGGTTTCCGCCGTGATACGCGTTTTa                                                                                                    <  1:788967/150‑1 (MQ=255)
                                                gCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTAc                                                                                                   <  2:500719/150‑1 (MQ=255)
                                                gCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTAc                                                                                                   >  1:806426/1‑150 (MQ=255)
                                                gCGTATTCAGCAGCTCTTCATAGCTGAAGGTCCAGACGCCGGTCGCATTGGAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAAGGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTAc                                                                                                   <  1:875088/150‑1 (MQ=255)
                                                 cGTATTCAGCAGCTCTTCATAGTTGAAGGGCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTAcc                                                                                                  <  2:1510288/150‑1 (MQ=255)
                                                  gaaTTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCTTCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGTTTGCCGCCGTGATACGCCTTTTACCg                                                                                                 <  1:101441/148‑1 (MQ=255)
                                                  gTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCg                                                                                                 <  1:996091/150‑1 (MQ=255)
                                                  gTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCg                                                                                                 >  1:569235/1‑150 (MQ=255)
                                                  gTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCg                                                                                                 >  1:320021/1‑150 (MQ=255)
                                                   tATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGt                                                                                                >  1:646700/1‑150 (MQ=255)
                                                   tATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGt                                                                                                >  2:1100827/1‑150 (MQ=255)
                                                    aTTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTc                                                                                               >  1:87116/1‑150 (MQ=255)
                                                         gcagcTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAgg                                                                                          >  1:1701205/1‑150 (MQ=255)
                                                         gcagcTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCGGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAgg                                                                                          >  1:1707705/1‑150 (MQ=255)
                                                          cagcTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGc                                                                                         >  1:165840/1‑150 (MQ=255)
                                                               ctTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTAt                                                                                    >  1:1385486/1‑150 (MQ=255)
                                                               ctTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTAt                                                                                    >  1:1712670/1‑150 (MQ=255)
                                                               ctTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTAt                                                                                    >  2:372189/1‑150 (MQ=255)
                                                                      gTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCca                                                                             >  1:272177/1‑150 (MQ=255)
                                                                       ttGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCcac                                                                            <  2:272177/150‑1 (MQ=255)
                                                                           aGGGCCCGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGAGGGCAATCAACATCCCCAGGCCACTCGCCTGGGTGTAAATGAAGACCTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTg                                                                        <  1:39654/150‑1 (MQ=255)
                                                                            ggTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGc                                                                       >  2:549405/1‑150 (MQ=255)
                                                                            ggTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTATACCGTCAGAGGCTTTATGCCCCCACAGTGc                                                                       >  1:330569/1‑150 (MQ=255)
                                                                             gTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCg                                                                      >  1:837896/1‑150 (MQ=255)
                                                                                 aGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCa                                                                  >  1:1185027/1‑150 (MQ=255)
                                                                                 aGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACAGTCAGAGGCTTTATGCCCCCACAGTGCGATCa                                                                  <  2:427117/150‑1 (MQ=255)
                                                                                   aCGCCGGTCGCATTGTAGTTAAAAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTTTAAATGAAGAACTTGGGTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAgg                                                                <  2:1670020/150‑1 (MQ=255)
                                                                                   aCGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGTCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAgg                                                                <  1:1505224/150‑1 (MQ=255)
                                                                                   aCGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAgg                                                                <  2:165840/150‑1 (MQ=255)
                                                                                    cGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGa                                                               <  2:1071994/150‑1 (MQ=255)
                                                                                    cGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGa                                                               <  2:196906/150‑1 (MQ=255)
                                                                                     gCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGaa                                                              <  1:372189/150‑1 (MQ=255)
                                                                                      ccGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAg                                                             <  1:1645118/150‑1 (MQ=255)
                                                                                      ccGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAg                                                             <  1:1400686/150‑1 (MQ=255)
                                                                                      ccGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCGGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAg                                                             >  1:841730/1‑150 (MQ=255)
                                                                                      ccGGTCGCATTGTAGTGAACAAAAACCAGCGCAAGGATGGCAATCTACATCACCAGGCCACTCGCCTGGGTGTATATGAAGAAGTTGGTTGCCGCCGTGATACGCGTTTTCCCGTCAGAGGCTTTATGCACCCACAGTGCGATAAGGAAg                                                             >  1:24309/1‑150 (MQ=255)
                                                                                            gCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATc                                                       <  1:1525424/150‑1 (MQ=255)
                                                                                            gCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATc                                                       <  1:1017228/150‑1 (MQ=255)
                                                                                            gCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTAACCGTCAGAGGCTTTATGCCCCCACAGTGGGATCAGGAAGTACATc                                                       >  2:1573804/1‑150 (MQ=255)
                                                                                            gCATTGTAGTGAACAAAAACCAGCGCAAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCAGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATc                                                       >  2:1580208/1‑150 (MQ=255)
                                                                                              aTTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCgg                                                     >  2:202842/1‑150 (MQ=255)
                                                                                               ttGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGc                                                    <  1:1661221/150‑1 (MQ=255)
                                                                                                  tAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCAcc                                                 <  2:1487225/150‑1 (MQ=255)
                                                                                                        aCAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCACCAGcatc                                           <  1:409304/150‑1 (MQ=255)
                                                                                                              aCCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCACCAGCATCATTTcc                                     <  1:1406222/150‑1 (MQ=255)
                                                                                                              aCCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCACCAGCATCATTTcc                                     <  2:1326406/150‑1 (MQ=255)
                                                                                                               ccAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCACCAGCATCATTTccc                                    <  2:591559/150‑1 (MQ=255)
                                                                                                                cAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCACCAGCATCATTTCCCa                                   <  1:510257/150‑1 (MQ=255)
                                                                                                                 aGCGCCAGGATTGCTATCAACATCACCAGTCCTCTCTCCTGGGTGTAAATTAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCGACAGTGCGATCAGGAAGTACATCGTCACCAGCATCATTTCCCag                                  <  2:1364443/150‑1 (MQ=255)
                                                                                                                        ggATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCACCAGCATCATTTCCCagaagaagc                           >  1:1473202/1‑149 (MQ=255)
                                                                                                                         gATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCACCAGCATCATTTCCCagaagaagaa                          >  2:1359200/1‑150 (MQ=255)
                                                                                                                             gCAATCAACATCACCAGGCCACTCGCCTGGATGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTCTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCACCAGCATCATTTCCCAGAAGAAGAAGAAc                      >  1:874689/1‑150 (MQ=255)
                                                                                                                              cAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGCACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCCCAGTGCGATCAGGAAGTTCATCGGCACCAGCATCATTTCCCAGAAGAAGAAGcaca                     >  1:849213/1‑150 (MQ=255)
                                                                                                                              cAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCACCAGCATCATTTCCCAGAAGAAGAAGAACa                     >  1:1399400/1‑150 (MQ=255)
                                                                                                                              cAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCACCAGCATCATTTCCCAGAAGAAGAAGAACa                     <  2:841730/150‑1 (MQ=255)
                                                                                                                               aaTCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCACCAGCATCATTTCCCAGAAGAAGAAGAACAg                    <  2:1215832/150‑1 (MQ=255)
                                                                                                                                    aCATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCACCAGCATCATTTCCCAGAAGAAGAAGAACAGGAACa               >  2:1316498/1‑150 (MQ=255)
                                                                                                                                      aTCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCACCAGCATCATTTCCCAGAAGAAGAAGAACAGGAACATg             <  2:928847/150‑1 (MQ=255)
                                                                                                                                       cacccAGGCGAGTGGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCACCAGCATCATTTCCCAGAAGAAGAAGAACAGGAACATGt            >  2:386084/4‑150 (MQ=255)
                                                                                                                                         cccAGGCGAGTGGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCACCAGCATCATTTCCCAGAAGAAGAAGAACAGGAACATGTCg          >  2:705012/2‑150 (MQ=255)
                                                                                                                                         aCCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCACCAGCATCATTTCCCAGAAGAAGAAGAACAGGAACATGTCg          >  1:675685/1‑150 (MQ=255)
                                                                                                                                            aGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCACCAGCATCATTTCCCAGAAGAAGAAGAACAGGAACATGTCGATg       >  2:706023/1‑150 (MQ=255)
                                                                                                                                             ggCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCACCAGCATCATTTCCCAGAAGAAGAAGAACa                     >  2:308559/1‑135 (MQ=255)
                                                                                                                                             ggCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCACCAGCATCATTTCCCAGAAGAAGAAGAACa                     <  1:308559/135‑1 (MQ=255)
                                                                                                                                             ggCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCACCAGCATCATTTCCCAGAAGAAGAAGAACAGGAACATGTCGATgg      <  2:421706/150‑1 (MQ=255)
                                                                                                                                             ggCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCACCAGCATCATTTCCCAGAAGAAGAAGAACAGGAACATGTCGATgg      >  2:603445/1‑150 (MQ=255)
                                                                                                                                                 aCTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCACCAGCATCATTTCCCAGAAGAAGAAGAACAGGAACATGTCGATGGCAAg  <  1:622002/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
CGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCACCAGCATCATTTCCCAGAAGAAGAAGAACAGGAACATGTCGATGGCAAG  >  NC_000913/2392354‑2392648

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: