Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,400,192 G→C 5.1% intergenic (‑27/+26) nuoG ← / ← nuoF NADH:ubiquinone oxidoreductase, chain G/NADH:ubiquinone oxidoreductase, chain F

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,400,1920GC 5.1% 392.7 / 7.3 114intergenic (‑27/+26)nuoG/nuoFNADH:ubiquinone oxidoreductase, chain G/NADH:ubiquinone oxidoreductase, chain F
Reads supporting (aligned to +/- strand):  ref base G (52/56);  new base C (4/2);  total (56/58)
Fisher's exact test for biased strand distribution p-value = 4.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

Only 100 of 124 total aligned reads displayed.

TTCCCAGCGCCGGATGCCAGCAAAAGTAAGGAATATCAAGGCCCAGAGACAGACAAGCTTCCAGCAGGTTGTCCGCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTGAATCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCC  >  NC_000913/2400045‑2400341
                                                                                                                                                   |                                                                                                                                                     
ttCCCAGCGCCGGATGCCAGCAAAAGTAAGGAATATCAAGGCCCAGAGACAGACAAGCTTCCAGCAGGTTGTCCGCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCagag                                                                                                                                                     >  2:28170/1‑150 (MQ=255)
ttCCCAGCGCCGGATGCCAGCAAAAGTAAGGAATATCAAGGCCCAGAGACAGACAAGCTTCCAGCAGGTTGTCCGCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCagag                                                                                                                                                     <  2:506158/150‑1 (MQ=255)
  cccAGCGCCGGATGCCAGCAAAAGTAAGGAATATCAAGGCCCAGAGACAGACAAGCTTCCAGCAGGTTGTCCGCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGAc                                                                                                                                                   <  2:806053/150‑1 (MQ=255)
   ccAGCGCCGGATGCCAGCAAAAGTAAGGAATATCAAGGCCCAGAGACAGACAAGCTTCCAGCAGGTTGTCCGCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACt                                                                                                                                                  >  1:790740/1‑150 (MQ=255)
       cgcCGGATGCCAGCAAAAGTAAGGAATATCAAGGCCCAGAGACAGACAAGCTTCCAGCAGGTTGTCCGCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGc                                                                                                                                              <  1:1635577/150‑1 (MQ=255)
         ccGGATGCCAGCAAAAGTAAGGAATATCAAGGCCCAGAGACAGACAAGCTTCCAGCAGGTTGTCCGCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGt                                                                                                                                            >  2:736820/1‑150 (MQ=255)
         ccGGATGCCAGCAAAAGTAAGGAATATCAAGGCCCAGAGACAGACAAGCTTCCAGCAGGTTGTCCGCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGt                                                                                                                                            >  2:1220350/1‑150 (MQ=255)
           ggATGCCAGCAAAAGTAAGGAATATCAAGGCCCAGAGACAGACAAGCTTCCAGCAGGTTGTCCGCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTa                                                                                                                                          >  1:924561/1‑150 (MQ=255)
            gATGCCAGCAAAAGTAAGGAATATCAAGGCCCAGAGACAGACAAGCTTCCAGCAGGTTGTCCGCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTaa                                                                                                                                         <  2:781593/150‑1 (MQ=255)
            gATGCCAGCAAAAGTAAGGAATATCAAGG‑CCAGAGACAGACAAGCTTCCAGCAGGTTGTCCGCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCTCTGACTGAgaaaactg                                                                                                                                        <  2:1388180/150‑8 (MQ=255)
              tGCCAGCAAAAGTAAGGAATAGCAAGGCCCTGAGAGAGACAAGCTTCCAGAAGGTTGTCCGCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGATTTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATc                                                                                                                                       <  1:314775/150‑1 (MQ=255)
                ccAGCAAAAGTAAGGAATATCAAGGCCCAGAGACAGACAAGCTTCCAGCAGGTTGTCCGCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGa                                                                                                                                     <  1:1437915/150‑1 (MQ=255)
                ccAGCAAAAGTAAGGAATATCAAGGCCCAGAGACAGACAAGCTTCCAGCAGGTTGTCCGCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGa                                                                                                                                     >  2:1615609/1‑150 (MQ=255)
                ccAGCAAAAGTAAGGAATATCAAGGCCCAGAGACAGACAACCTTCCAGCAGGTTGTCCGCTCCGTTGACATCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTacacga                                                                                                                                     >  2:1184556/1‑145 (MQ=255)
                  aGCAAAAGTAAGGAATATCAAGGCCCAGAGACAGACAAGCTTCCAGCAGGTTGTCCGCTCCGTTGCCCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTACAGTTTTCTCAGTCAGAGAATGAGCGTTAATCTaaa                                                                                                                                   >  1:1320189/1‑150 (MQ=255)
                   gCAAAAGTAAGGAATATCAAGGCCCAGAGACAGACAAGCTTCCAGCAGGTTGTCCGCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAAt                                                                                                                                  <  1:412122/150‑1 (MQ=255)
                     aaaaGTAAGGAATATCAAGGCCCAGAGACAGACAAGCTTCCAGCAGGTTGTCCGCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTc                                                                                                                                >  2:1241240/1‑150 (MQ=255)
                         ttAAGGAATATCAAGGCCCAGAGACAGACAAGCTTCCAGCAGGTTGTCCGCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGtt                                                                                                                            <  1:661588/149‑1 (MQ=255)
                               aaTATCAAGGCCCAGAGACAGACAAGCTTCCAGCAGGTTGTCCGCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCAgc                                                                                                                      <  2:1168300/150‑1 (MQ=255)
                                   acAAGGCCCAGAGACAGACAAGCTTCCAGCAGGTTGTCCGCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAAATGAAGCCAATAGCATCCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTAGGTTACCAGCGctc                                                                                                                  >  1:205858/2‑150 (MQ=255)
                                    cAAGGCCCAGAGACAGACAAGCTTCCAGCAGGTTGTCCGCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGctct                                                                                                                 <  1:1270467/150‑1 (MQ=255)
                                    cAAGGCCCAGAGACAGACAAGCTTCCAGCAGGTTGTCCGCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGctct                                                                                                                 >  2:1366865/1‑150 (MQ=255)
                                      aGGCCCAGAGACAGACAAGCTTCCAGCAGGTTGTCCGCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCttt                                                                                                               <  1:1206468/150‑1 (MQ=255)
                                      aGGCACAGAGACAGACAAGCTTCCAGCAGGTTGTCCGCTCCGTTGATCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCttt                                                                                                               <  1:327551/150‑1 (MQ=255)
                                        gCCCAGAGACAGACAAGCTTCCAGCAGGTTGTCCGCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTca                                                                                                             >  2:762666/1‑150 (MQ=255)
                                         cccAGAGACAGACAAGCTTCCAGCAGGTTGTCCGCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTcag                                                                                                            <  2:801825/150‑1 (MQ=255)
                                         cccAGAGACAGACAAGCTTCCAGCAGGTTGTCCGCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTcag                                                                                                            <  2:907678/150‑1 (MQ=255)
                                          ccAGAGACAGACAAGCTTCCCGCAGGTTGTCCGCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTcagc                                                                                                           >  1:1035807/1‑150 (MQ=255)
                                             gagaCAGACAAGCTTCCAGCAGGTTGTCCGCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTCAGCAgg                                                                                                        <  1:28170/150‑1 (MQ=255)
                                             gagaCAGACAAGCTTCCAGCAGGTTGTCCGCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTCAGCAgg                                                                                                        <  2:1471291/150‑1 (MQ=255)
                                             gagaCAGACAAGCTTCCAGCAGGTTGTCCGCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTCAGCAgg                                                                                                        >  2:1598339/1‑150 (MQ=255)
                                                 cagacaAGCTTCCAGCAGGTTGTCCGCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCg                                                                                                    <  1:1264596/150‑1 (MQ=255)
                                                   gacaAGCTTCCAGCAGGTTGTCCGCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGc                                                                                                  >  1:1144373/1‑150 (MQ=255)
                                                     ctatcTTCCAGCAGGTTGTCCGCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTg                                                                                                <  1:1234629/146‑1 (MQ=255)
                                                     caAGCTTCCAGCAGGTTGTCCGCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTATCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTg                                                                                                >  2:920947/1‑150 (MQ=255)
                                                     caAGCTTCCAGCAGGTTGTCCGCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTg                                                                                                <  1:1574148/150‑1 (MQ=255)
                                                     ataGCTTCCAGCTGGTTGTCCGCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCTTGCTTCCAGTTTTCTCAGTCTGAGACTGAGGGTTAATCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTg                                                                                                <  1:1339075/148‑1 (MQ=255)
                                                        gCTTCCAGCAGGTTGTCCGCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTGAAt                                                                                             <  1:1389373/150‑1 (MQ=255)
                                                          ttCCAGCAGGTTGTCCGCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTGAATcc                                                                                           <  1:283799/150‑1 (MQ=255)
                                                           tCCAGCAGGTTGTCCGCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTGAATccc                                                                                          <  1:1086898/150‑1 (MQ=255)
                                                           tCCAGCAGGTTGTCCGCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTGAATccc                                                                                          >  2:1323173/1‑150 (MQ=255)
                                                           tCCAGCAGGATGTCCTCTCCGTTGACCTCGTATTATTTGCCGTCTACATGAATTGAAGCCATTAGGATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTTCCAGCGCTCTTTCAGCAGTTTCGGCTGAATccc                                                                                          <  2:1320189/150‑1 (MQ=255)
                                                             cagcagGTTGTCCGCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCt                                                                                                 <  2:215324/141‑1 (MQ=255)
                                                             cagcagGTTGTCCGCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCt                                                                                                 >  1:215324/1‑141 (MQ=255)
                                                             cagcagGTTGTCCGCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTGAATCCCAt                                                                                        <  1:1276778/150‑1 (MQ=255)
                                                              agcagGTTGTCCGCTCCGTTGACCTCGTATTCTTTGCCGTCTACTTGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTCAGCACGTTCGGCTGAATCCCAtt                                                                                       >  1:1584310/1‑150 (MQ=255)
                                                                  ggTTGTCCGCTCCGTTGCCCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTCCGCAGGTTCGGCTGCATCCCATTAATc                                                                                   >  2:849510/1‑150 (MQ=255)
                                                                  ggTTGTCCGCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCGGAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTGAATCCCATTAATc                                                                                   <  2:1483321/150‑1 (MQ=255)
                                                                  ggTTGTCCGCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTGAATCCCATTAATc                                                                                   >  1:215694/1‑150 (MQ=255)
                                                                   gTTGTCCGCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTGAATCCCATTAATCa                                                                                  <  1:1323173/150‑1 (MQ=255)
                                                                       tCCGCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTGAATCCCATTAATCAAATg                                                                              <  1:1366865/150‑1 (MQ=255)
                                                                       tCCGCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTGAATCCCATTAATCAAATg                                                                              >  2:399098/1‑150 (MQ=255)
                                                                         cGCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTGAATCCCATTAATCAAATggg                                                                            <  2:1382802/150‑1 (MQ=255)
                                                                         cGCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTGAATCCCATTAATCAAATggg                                                                            <  2:165678/150‑1 (MQ=255)
                                                                         cGCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTGAATCCCATTAATCAAATggg                                                                            <  2:1282629/150‑1 (MQ=255)
                                                                           cTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCGGCGCTCTTTCAGCAGGTTCGGCTGAATCCCATTAATCAAATGGGTa                                                                          >  2:289964/1‑150 (MQ=255)
                                                                           cTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTGAATCCCATTAATCAAATGGGTa                                                                          <  2:1600484/150‑1 (MQ=255)
                                                                           cTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCAAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTGAATCCCATTAATCAAATGGGTa                                                                          <  2:1105275/150‑1 (MQ=255)
                                                                            tCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTGAATCCCATTAATCAAATGGGTAt                                                                         <  2:1144373/150‑1 (MQ=255)
                                                                            tCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTGAATCCCATTAATCAAATGGGTAt                                                                         >  1:793856/1‑150 (MQ=255)
                                                                             ccGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTGAATCCCATTAATCAAATGGGTAtt                                                                        <  2:1316448/150‑1 (MQ=255)
                                                                               gTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTGAATCCCATTAATCAAATGGGTATTGc                                                                      <  1:1615609/150‑1 (MQ=255)
                                                                                  gACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTACGGCTGAATCCCATTAATCAAATGGGTATTGCTGa                                                                   >  2:716365/1‑150 (MQ=255)
                                                                                   aCCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTGAATCCCATTAATCAAATGGGTATTGCTGaa                                                                  <  2:924561/150‑1 (MQ=255)
                                                                                   aCCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTGAATCCCATTAATCAAATGGGTATTGCTGaa                                                                  >  2:1061516/1‑150 (MQ=255)
                                                                                   aCCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTGAATCCCATTAATCAAATGGGTATTGCTGaa                                                                  >  1:327126/1‑150 (MQ=255)
                                                                                     cTCGTATTCTTTGCCGTCTACATTATTTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGAATGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTGAATCCCATTAATCAAATGGGTATTGCTGAACg                                                                <  2:1048627/150‑1 (MQ=255)
                                                                                     cTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTGAATCCCATTAATCAAATGGGTATTGCTGAACg                                                                >  1:116369/1‑150 (MQ=255)
                                                                                             cTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTGAATCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTg                                                        <  1:1124110/150‑1 (MQ=255)
                                                                                             cTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTGAATCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTg                                                        >  1:1362342/1‑150 (MQ=255)
                                                                                             cTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTGAATCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTg                                                        <  1:1598339/150‑1 (MQ=255)
                                                                                                 gCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTGAATCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTc                                                    >  2:342890/1‑150 (MQ=255)
                                                                                                 gCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTGAATCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTc                                                    >  2:669822/1‑150 (MQ=255)
                                                                                                    gTCTACATGAATTGTAGCGATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTGAATCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCg                                                 <  2:653492/150‑1 (MQ=255)
                                                                                                        aCATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTGAATCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTc                                             >  2:727506/1‑150 (MQ=255)
                                                                                                                   aGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTGAATCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTcgc                                  <  2:116369/150‑1 (MQ=255)
                                                                                                                      cATTAGCATGCTTCCAGTTTTCTCTGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTGAATCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGa                               >  2:1534698/1‑150 (MQ=255)
                                                                                                                        ttAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTGAATCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGaaa                             <  1:669822/150‑1 (MQ=255)
                                                                                                                                cTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTGAATCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGAt                     >  2:1362441/1‑150 (MQ=255)
                                                                                                                                 ttCCAGTTTTCTCTGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTGAATCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATg                    <  1:1534698/150‑1 (MQ=255)
                                                                                                                                  tcaaGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTAGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTGAATCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGATATATTTGATgg                   >  2:150856/4‑150 (MQ=255)
                                                                                                                                   ccAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTGAATCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGgc                  >  2:1164106/1‑150 (MQ=255)
                                                                                                                                     aGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTGAATCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGgcgc                >  1:1317693/1‑150 (MQ=255)
                                                                                                                                       ttttCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTGAATCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGctc              <  2:790740/150‑1 (MQ=255)
                                                                                                                                       aacgctcAGTCTCTGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTGAATCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGctc              >  2:1229851/5‑150 (MQ=255)
                                                                                                                                        tttCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTGAATCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGctct             <  1:736820/150‑1 (MQ=255)
                                                                                                                                        tttCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCAGAATCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGCTTCCCGCCTCAAATTCTTCGGGGAAATATTTGATGGCGctgt             >  1:272495/1‑148 (MQ=255)
                                                                                                                                        acgctcAGTCTCTGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTGAATCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATAATTGATGGCGctct             >  2:253026/4‑150 (MQ=255)
                                                                                                                                         ttCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTGAATCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTg            >  2:464711/1‑150 (MQ=255)
                                                                                                                                          tctcAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTGAATCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGAGCTCTGt           >  2:695479/1‑150 (MQ=255)
                                                                                                                                          gctcAGTCTCTGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTGAATCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGt           >  1:655740/2‑150 (MQ=255)
                                                                                                                                           ctcAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTGAATCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTa          >  2:500996/1‑150 (MQ=255)
                                                                                                                                             cagtcagAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTGAATCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAAc        >  1:91362/1‑150 (MQ=255)
                                                                                                                                             cagtcagAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTGAATCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAAc        <  1:100213/150‑1 (MQ=255)
                                                                                                                                              agtcagAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTGAATCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACg       <  1:762666/150‑1 (MQ=255)
                                                                                                                                              agtcagAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTGAATCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACg       >  1:64487/1‑150 (MQ=255)
                                                                                                                                               gtcagAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTGAATCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGAGCTCTGTAACgg      >  1:1225223/1‑150 (MQ=255)
                                                                                                                                               gtcagAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTGAATCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGAGCTCTGTAACgg      >  1:1225216/1‑150 (MQ=255)
                                                                                                                                                  agagACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTGAATCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTc   >  1:411464/1‑150 (MQ=255)
                                                                                                                                                   gagaCTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTGAATCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTcc  <  2:887027/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
TTCCCAGCGCCGGATGCCAGCAAAAGTAAGGAATATCAAGGCCCAGAGACAGACAAGCTTCCAGCAGGTTGTCCGCTCCGTTGACCTCGTATTCTTTGCCGTCTACATGAATTGTAGCCATTAGCATGCTTCCAGTTTTCTCAGTCAGAGACTGAGCGTTAATCGAAATTCGGTTACCAGCGCTCTTTCAGCAGGTTCGGCTGAATCCCATTAATCAAATGGGTATTGCTGAACGGCTGTTTGATTCCCGCCTCAAATTCTTCGCGGAAATATTTGATGGCGCTCTGTAACGGCTCC  >  NC_000913/2400045‑2400341

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑