Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 212,728 G→C 6.0% G133A (GGC→GCC)  tilS → tRNA(Ile)‑lysidine synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913212,7280GC 6.0% 400.3 / 12.2 117G133A (GGC→GCC) tilStRNA(Ile)‑lysidine synthetase
Reads supporting (aligned to +/- strand):  ref base G (59/51);  new base C (3/4);  total (62/55)
Fisher's exact test for biased strand distribution p-value = 7.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.19e-01

Only 100 of 124 total aligned reads displayed.

GCAAGAAGGACTGGGCATTGAGGCCCAGGCGCGGCAGGCACGTTATCAGGCATTTGCCCGCACCTTGTTGCCCGGTGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCCGCACGCGGGGGGAACTGGTGCAGTGGGCGCGTCAGTATGATTTACGCTGGATTGAAGACGAAAGTAATCAGGACG  >  NC_000913/212579‑212877
                                                                                                                                                     |                                                                                                                                                     
gcAAGAAGGACTGGGCATTGAGGCCCAGGCGCGGCAGGCACGTTATCAGGCATTTGCCCGCACCTTGTTGCCCGGTGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTgg                                                                                                                                                       >  2:796237/1‑150 (MQ=255)
  aagaagGACTGGGCATTGAGGCCCAGGCGCGGCAGGCACGTTATCAGGCATTTGCCCGCCCCTTGTTGCCCGGTGAAGTGCTGGTCACCGTGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGcc                                                                                                                                                     >  2:756341/1‑150 (MQ=255)
      agGACTGGGCATTGAGGCCCAGGCGCGGTAGGCACGTTATCAGGCATTTGCCCGCACCTTGTTGCCCGGTGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGc                                                                                                                                                 <  2:435447/150‑1 (MQ=255)
      agGACTGGGCATTGAGGCCCAGGCGCGGCAGGCACGTTATCAGGCATTTGCCCGCACCTTGTTGCCCGGTGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGc                                                                                                                                                 >  1:254932/1‑150 (MQ=255)
       ggACTGGGCATTGAGGCCCAGGCGCGGCAGGCACGTTATCAGGCATTTGCCCGCACCTTGTTGCCCGGTGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGcc                                                                                                                                                >  1:557110/1‑150 (MQ=255)
        gACTGGGCATTGAGGCCCAGGCGCGGCAGGCACGTTATCAGGCATTTGCCCGCACCTTGTTGCCCGGTGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCg                                                                                                                                               >  2:23209/1‑150 (MQ=255)
         aCTGGGCATTGAGGCCCAGGCGCGGCAGGCACGTTATCAGGCATTTGCCCGCACCTTGTTGCCCGGTGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCgg                                                                                                                                              <  1:526665/150‑1 (MQ=255)
         aCTGGGCATTGAGGCCCAGGCGCGGCAGGCACGTTATCAGGCATTTGCCCGCACCTTGTTGCCCGGTGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCgg                                                                                                                                              >  1:1463364/1‑150 (MQ=255)
          cTGGGCATTGCGGCCCAGGCGCGGCAGGCACGTTATCAGGCATTTGCCCGCACCTTGGTGCCCGGTGAAGGGCGGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCggg                                                                                                                                             <  2:1489983/150‑1 (MQ=255)
          cTGGGCATTGAGGCCCAGGCGCGGCAGGCACGTTATCAGGCATTTGCCCGCACCTTGTTGCCCGGTGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCggg                                                                                                                                             <  1:1344319/150‑1 (MQ=255)
          cTGGGCATTGAGGCCCAGGCGCGGCAGGCACGTTATCAGGCATTTGCCCGCACCTTGTTGCCCGGTGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCggg                                                                                                                                             <  2:488375/150‑1 (MQ=255)
          cTGGGCATTGAGGCCCAGGCGCGGCAGGCACGTTATCAGGCATTTGCCCGCACCTTGTTGCCCGGTGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCggg                                                                                                                                             <  2:392351/150‑1 (MQ=255)
          cTGGGCATTGAGGCCCAGGCGCGGCAGGCACGTTATCAGGCATTTGCCCGCACCTTGTTGCCCGGTGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCggg                                                                                                                                             <  1:1533514/150‑1 (MQ=255)
           tGGGCATTGAGGCCCAGGCGCGGCAGGCACGTTATCAGGCATTTGCCCGCACCTTGTTGCCCGGTGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGc                                                                                                                                            >  1:646368/1‑150 (MQ=255)
            gggCATTGAGGCCCAGGCGCGGCAGGCACGTTATCAGGCATTTGCCCGCACCTTGTTGCCCGGTGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTGAAACGCGGCAGTGGCCCTGCCGGGCt                                                                                                                                           >  1:1553801/1‑150 (MQ=255)
            gggCATTGAGGCCCAGGCGCGGCAGGCACGTTATCAGGCATTTGCCCGCACCTTGTTGCCCGGTGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGGGCCACTGCCgcgtt                                                                                                                                           <  1:1562027/150‑5 (MQ=255)
            gggCATTGAGGCCCAGGCGCGGCAGGCACGTTATCAGGCATTTGCCCGCACCTTGTTGCCCGGTGAAGTGCTGGGCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGGGCCACTGCCgcgtt                                                                                                                                           <  1:1442516/150‑5 (MQ=255)
             ggCATTGAGGCCCAGGCGCGGCAGGCACGTTATCAGGCATTTGCCCGCACCTTGTTGCCCGGGGAAGTGCTGGGCACCGCGCAAAATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCtt                                                                                                                                          <  2:1339924/150‑1 (MQ=255)
                  tGAGGCCCAGGCGCGGCAGGCACGTTATCAGGCATTTGCCCGCACCTTGTTGCCCGGGGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGGGCCACTGCCGCGttttagcg                                                                                                                                     <  1:1482008/150‑5 (MQ=255)
                   gAGGCCCAGGCGCGGCAGGCACGTTATCAGGCATTTGCCCGCACCTTGTTGCCCGGTGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCTGCt                                                                                                                                    >  2:293418/1‑150 (MQ=255)
                    aGGCCCAGGCGCGGCAGGCACGTTATCAGGCATTTGCCCGCACCTTGTTGCCCGGTGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTa                                                                                                                                   <  2:278849/150‑1 (MQ=255)
                     ggCCCAGGCGCGGCAGGCACGTTATCAGGCATTTGCCCGCACCTTGTTGCCCGGTGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTAt                                                                                                                                  >  1:468421/1‑150 (MQ=255)
                      gCCCAGGCGCGGCAGGCACGTTATCAGGCATTTGCCCGCACCTTGTTGCCCGGTGAAGTGCTGGTCACCGCGCAACATCTCGCCGATCAATGTGACACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATg                                                                                                                                 >  1:302281/1‑150 (MQ=255)
                           ggCGCGGCAGGCACGTTATCAGGCATTTGCCCGCACCTTGTTGCCCGGTGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGa                                                                                                                            >  2:927113/1‑150 (MQ=255)
                            gcgcGGCAGGCACGTTATCAGGCATTTGCCCGCACCTTGTTGCCCGGTGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGaa                                                                                                                           <  2:1020129/150‑1 (MQ=255)
                               cgGCAGGCACGTTATCAGGCATTTGCCCGCACCTTGTTGCCCGGTGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGtc                                                                                                                        <  2:940217/150‑1 (MQ=255)
                                 gcaggcaCGTTATCAGGCATTTGCCCGCACCTTGTTGCCCGGTGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGtctc                                                                                                                      >  2:1718720/1‑150 (MQ=255)
                                    ggcaCGTTATCAGGCATTTGCCCGCACCTTGTTGCCCGGTGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGa                                                                                                                   >  1:803540/1‑150 (MQ=255)
                                      caCGTTATCAGGCATTTGCCCGCACCTTGTTGCCCGGTGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGt                                                                                                                 >  1:963637/1‑150 (MQ=255)
                                       aCGTTATCAGGCATTTGCCCGCACCTTGTTGCCCGGTGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGtt                                                                                                                >  2:138891/1‑150 (MQ=255)
                                       aCGTTATCAGGCATTTGCCCGCACCTTGTTGCCCGGTGAAGTGCTGGTCACCGCGCAACATCTAGACGATCAATGTGAAACCTTTCTGCTGGCGCGAAAACGCGGCAGTGGACCTGCCGGGCTTTCGGGTATTGAGTAAGTCTCGGAGtt                                                                                                                >  1:1045448/1‑150 (MQ=255)
                                        cGTTATCAGGCATTTGCCCGCACCTTGTTGCCCTGTGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAACCGCGGCCGTGGCCCTGCCGGGCTTTCGGCTATGGCTGAAGTCTCGGAGttt                                                                                                               >  1:81216/1‑150 (MQ=255)
                                        cGTTATCAGGCATTTGCCCGCACCTTGTTGCCCGGTGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGttt                                                                                                               <  2:409840/150‑1 (MQ=255)
                                         gTTATCAGGCATTTGCCCGCACCTTGTTGCCCGGTGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTg                                                                                                              <  2:1207241/150‑1 (MQ=255)
                                         gTTATCAGGCATTTGCCCGCACCTTGTTGCCCGGTGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTg                                                                                                              <  2:646368/150‑1 (MQ=255)
                                            aTCAGGCATTTGCCCGCACCTTGTTGCCCGGTGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCg                                                                                                           <  1:988774/150‑1 (MQ=255)
                                            aTCAGGCATTTGCCCGCACCTTGTTGCCCGGTGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCg                                                                                                           <  2:803540/150‑1 (MQ=255)
                                            aTCAGGCATTTGCCCGCACCTTGTTGCCCGGCGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCg                                                                                                           >  1:1626453/1‑150 (MQ=255)
                                               aGGCATTTGCCCGCACCTTGTTGCCCGGTGAAGTGCTGGTCACCGCGAAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCAGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGaa                                                                                                        >  1:1047469/1‑150 (MQ=255)
                                                 gCATTTGCCCGCACCTTGTTGCCCGGTGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAAcg                                                                                                      >  2:757970/1‑150 (MQ=255)
                                                 gCATTTGCCCGCACCTTGTTGCCCGGTGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAAcg                                                                                                      >  2:369488/1‑150 (MQ=255)
                                                      tGCCCGCACCTTGTTGCCCGGTGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCTGGAACGCGGCt                                                                                                 >  2:681219/1‑150 (MQ=255)
                                                      tGCCCGCACCTTGTTGCCCGGTGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCt                                                                                                 <  2:616079/150‑1 (MQ=255)
                                                      tGCCCGCACCTTGTTGCCCGGGGAAGTGCTGGTCACCGCGCAACATCTCGACGATCACTGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCt                                                                                                 <  1:332420/150‑1 (MQ=255)
                                                       gCCCGCACCTTGTTGCCCGGTGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGGGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCtt                                                                                                <  1:557885/150‑1 (MQ=255)
                                                             aCCTTGTTGCCCGGTGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGc                                                                                          <  1:858384/150‑1 (MQ=255)
                                                                   ttgCCCGGTGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTg                                                                                    <  2:1169608/150‑1 (MQ=255)
                                                                    tgCCCGGTGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGc                                                                                   >  1:1337470/1‑150 (MQ=255)
                                                                     gCCCGGTGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCt                                                                                  >  2:1451527/1‑150 (MQ=255)
                                                                     gCCCGGTGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTAGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTgat                                                                                  >  2:488608/1‑148 (MQ=255)
                                                                       ccGGTGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCg                                                                                >  2:1615132/1‑150 (MQ=255)
                                                                         ggTGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGcc                                                                              <  2:44302/150‑1 (MQ=255)
                                                                          gTGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGccc                                                                             <  1:1092370/150‑1 (MQ=255)
                                                                           tGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCCg                                                                            >  1:289801/1‑150 (MQ=255)
                                                                              aGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCCGCAc                                                                         >  2:133149/1‑150 (MQ=255)
                                                                                  gtGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCCGCACGCgg                                                                     <  2:1347087/149‑1 (MQ=255)
                                                                                    ggTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCCGCACGCgggg                                                                   <  2:1534515/150‑1 (MQ=255)
                                                                                    ggTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCCGCACGCgggg                                                                   >  2:1002141/1‑150 (MQ=255)
                                                                                     gTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGGGGCCCTGCCGGGCGGTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCCGCACGCggggg                                                                  <  2:1139354/150‑1 (MQ=255)
                                                                                      tCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCCGCACGCgggggg                                                                 <  2:150606/150‑1 (MQ=255)
                                                                                        aCCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCCGCACGCGGGGGGaa                                                               >  2:486135/1‑150 (MQ=255)
                                                                                          cgcgCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCCGCACGCGGGGGGAACt                                                             <  1:23209/150‑1 (MQ=255)
                                                                                          cgcgCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCCGCACGCGGGGGGAACt                                                             >  1:1550940/1‑150 (MQ=255)
                                                                                            cgcAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCCGCACGCGGGGGGAACTgg                                                           >  2:139043/1‑150 (MQ=255)
                                                                                               aaCATCTCGACGATCAATGTGAAACCTAGCTGCTGGCGCTAACACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGACGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCCGCACGCGGGGGGAACTGGTGc                                                        <  1:323682/150‑1 (MQ=255)
                                                                                                 cATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCCGCACGCGGGGGGAACTGGTGCAg                                                      <  2:963637/150‑1 (MQ=255)
                                                                                                  aTCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCCGCACGCGGGGGGAACTGGTGCAGt                                                     <  1:710169/150‑1 (MQ=255)
                                                                                                   tctcGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCCGCACGCGGGGGGAACTGGTGCAGTg                                                    <  1:1393961/150‑1 (MQ=255)
                                                                                                    ctcGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCCGCACGCGGGGCGAACTGGTGCAGTgg                                                   >  2:225522/1‑150 (MQ=255)
                                                                                                    cacgacgaTCAATGAGAAACCATTCTGCTGGCGCTAAACCGCGCCAGTGGCCCTGCCGGGCTTTCGGATATGGCGGAAGTCTCGCAGATTCCCCGAACGAGGCTTATTCGCCCGTTGCTCGCCCGCACGCGGTGGGAACTGGTGCAGTgg                                                   >  2:228154/3‑150 (MQ=255)
                                                                                                      cgacgaTCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCCGCACGCGGGGGGAACTGGTGCAGTGGgc                                                 >  2:137935/1‑150 (MQ=255)
                                                                                                      cgacgaTCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCCGCACGCGGGGGGAACTGGTGCAGTGGgc                                                 >  1:1620711/1‑150 (MQ=255)
                                                                                                       gacgaTCAATGTGAAACCTTTCTGCTGGCGCTAAACCGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCTGGAACGCGGCTTATTCGCCCGTTGCTCGCCCGCACGCGGGGGGAACTGGTGCAGTGGgcg                                                <  1:681219/150‑1 (MQ=255)
                                                                                                        acgaTCAATGTGAACCCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCCGCCCGCGGGGGGAACTGGTGCAGTGGgcgc                                               >  2:70020/1‑150 (MQ=255)
                                                                                                         cgaTCAATGTGAATCCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCCGCACGCGGGGGGAACTGGTGCAGGGGGcgcg                                              >  2:904795/1‑150 (MQ=255)
                                                                                                         cgaTCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGATATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCCGCACGCGGGGGGAACTGGTGCAGTGGGcgcg                                              <  2:1600260/150‑1 (MQ=255)
                                                                                                            tCAATGTGAAACCTTTCTGCTGGCGCTAAACCGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCCGCACGCGGGGGGAACTGGTGCAGTGGGCGCGTCa                                           <  2:433442/150‑1 (MQ=255)
                                                                                                              aaTGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCCGCACGCGGGGGGAACTGGTGCAGTGGGCGCGTCAGt                                         >  2:50417/1‑150 (MQ=255)
                                                                                                                  tgAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCCGCACGCGGGGGGAACTGGTGCAGTGGGCGCGTCAGTATGa                                     >  1:1648624/1‑150 (MQ=255)
                                                                                                                    aaaCCTTTCTGCTGGCGCTAAACCGCGGCAGTGGCCCTGCCGGGCTTTCGTCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCCTCACGCGGGGGGACCTGGAGCAGTGGGCGCGTCAGTATGAtt                                   >  1:282759/1‑150 (MQ=255)
                                                                                                                     aaCCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCCGCACGCGGGGGGAACTGGTGCAGTGGGCGCGTCAGTATGAttt                                  <  1:133149/150‑1 (MQ=255)
                                                                                                                       ccTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCCGCACGCGGGGGGAACTGGTGCAGTGGGCGCGTCAGTATGATTTAc                                >  1:293034/1‑150 (MQ=255)
                                                                                                                        cTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCGTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCCGCACGCGGGGGGAACTGGTGCAGTGGGCGCGTCAGTATGATTTACg                               <  2:1626453/150‑1 (MQ=255)
                                                                                                                        cTTTCGGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGGCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCCGCACGCGGGGGGAACTGGTGCAGGGGGCGCGTCAGTATGATTTACg                               <  1:308896/150‑1 (MQ=255)
                                                                                                                          ttCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCCGCACGCGGGGGGAACTGGTGCAGTGGGCGCGTCAGTATGATTTACGCt                             <  1:429478/150‑1 (MQ=255)
                                                                                                                           tCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGCACGCGGCTTATTCGCCCGTTGCTCGCCCGCACGCGGGGGGAGCTGGTGCAGTGGGCGCGTCAGTATGATTTACGCTg                            >  1:1706916/1‑150 (MQ=255)
                                                                                                                           tCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCCGCACGCGGGGGGAACTGGTGCAGTGGGCGCGTCAGTATGATTTACGCTg                            <  1:796237/150‑1 (MQ=255)
                                                                                                                               ctgGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCCGCACGCGGGGGGAACTGGTGCAGTGGGCGCGTCAGTATGATTTACGCTGGAtt                        >  1:1667113/1‑150 (MQ=255)
                                                                                                                                   cgcTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCCGCACGCGGGGGGAACTGGTGCAGTGGGCGCGTCAGTATGATTTACGCTGGATTGAAg                    >  1:1349228/1‑150 (MQ=255)
                                                                                                                                   cgcTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTAGCCCGCACGCGGGGGGAACTGGTGCAGTGGGCGCGTCAGTATGATTTACGCTGGATTGAAg                    >  2:1078369/1‑150 (MQ=255)
                                                                                                                                    gcTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCCGCACGCGGGGGGAACTGGTGCAGTGGGCGCGTCAGTATGATTTACGCTGGATTGAAGa                   <  2:1545394/150‑1 (MQ=255)
                                                                                                                                     cTAAACCGCGGCAGGGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCGCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCCGCACGCGGGGGGAACTGGTGCAGTGGGCGCGTCAGTATGATTTACGCTGGATTGAAGAc                  <  2:289801/150‑1 (MQ=255)
                                                                                                                                          gcccgGCAGGGCCACTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCCGCACGCGGGGGGAACTGGTGCAGTGGGCGCGTCAGTATGATTTACGCTGGATTGAAGACGAAAg             >  1:396521/4‑150 (MQ=255)
                                                                                                                                           cccgGCAGGGCCACTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCCGCACGCGGGGGGAACTGGTGCAGTGGGCGCGTCAGTATGATTTACGCTGGATTGAAGACGAAAGt            >  1:899618/3‑150 (MQ=255)
                                                                                                                                           cccgGCAGGGCCACTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCCGCACGCGGGGGGAACTGGTGCAGTGGGCGCGTCAGTATGATTTACGCTGGATTGAAGACGAAAGt            >  1:1185295/3‑150 (MQ=255)
                                                                                                                                                cAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCCGCACGCGGGGGGAACTGGTGCAGTGGGCGCGTCAGTATGATTTACGCTGGATTGAAGACGAAAGTAATCa       >  1:659663/1‑150 (MQ=255)
                                                                                                                                                    ggCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCCGCACGCGGGGGGAACTGGTGCAGTGGGCGCGTCAGTATGATTTACGCTGGATTGAAGACGAAAGTAATCAGgac   >  2:1328358/1‑150 (MQ=255)
                                                                                                                                                    ggCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCCGCACGCGGGGGGAACTGGTGCAGTGGGCGCGTCAGTATGATTTACGCTGGATTGAAGACGAAAGTAATCAGgac   >  2:1363198/1‑150 (MQ=255)
                                                                                                                                                     gCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCCGCACGCGGGGGGAACTGGTGCAGTGGGCGCGTCAGTATGATTTACGCTGGATTGAAGACGAAAGTAATCAGgacg  <  1:50417/150‑1 (MQ=255)
                                                                                                                                                     gCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGATTATTCGCCCGTTGCTCGCCCGCACGCGGGGGGAACTGGTGCAGTGGGCGCGTCAGTATGATTTACGCTGGATTGAAGACGAAAGTAATCAGgacg  <  1:1082131/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GCAAGAAGGACTGGGCATTGAGGCCCAGGCGCGGCAGGCACGTTATCAGGCATTTGCCCGCACCTTGTTGCCCGGTGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCCGCACGCGGGGGGAACTGGTGCAGTGGGCGCGTCAGTATGATTTACGCTGGATTGAAGACGAAAGTAATCAGGACG  >  NC_000913/212579‑212877

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑