Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,412,911 G→T 5.1% A74A (GCC→GCA yfbV ← UPF0208 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,412,9110GT 5.1% 422.7 / 9.0 137A74A (GCC→GCAyfbVUPF0208 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (70/59);  new base T (3/4);  total (74/63)
Fisher's exact test for biased strand distribution p-value = 7.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.85e-01

Only 100 of 139 total aligned reads displayed.

CAACGGGTGCCAACACCTGTCCAGACTCCTGCAATTTACCGCGAACTTCATAAAACCAGTTAAGGATTGCAGGGGGTAATGGCGTGACAGAACGCTTGCCCAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCA  >  NC_000913/2412763‑2413060
                                                                                                                                                    |                                                                                                                                                     
 aaCGGGTGCCAACACCTGTCCAGACTCCTGCAATTTACCGCGAACTTCATAAAACCAGTTAAGGATTGCAGGGGGTAATGGCGTGACAGAACGCTTGCCCAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGc                                                                                                                                                     <  2:1045730/150‑1 (MQ=255)
   cGGGTGCCAACACCTGTCCAGACTCCTGCAATTTACCGCGAACTTCATAAAACCAGTTAAGGATTGCAGGGGGTAATGGCGTGACAGAACGCTTGCCCAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCaa                                                                                                                                                   >  1:1385524/1‑150 (MQ=255)
     ggTGCCAACACCTGTCCAGACTCCTGCAATTTACCGCGAACTTCATAAAACCAGTTAAGGATTGCAGGGGGTAATGGCGTGACAGAACGCTTGCCCAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACt                                                                                                                                                 >  2:1334982/1‑150 (MQ=255)
     ggTGCCAACACCTGTCCAGACTCCTGCAATTTACCGCGAACTTCATAAAACCAGTTAAGGATTGCAGGGGGTAATGGCGTGACAGAACGCTTGCCCAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACt                                                                                                                                                 >  2:774872/1‑150 (MQ=255)
      gTGCCAACACCTGTCCAGACTCCTGCAATTTACCGCGAACTTCATAAAACCAGTTAAGGATTGCAGGGGGTAATGGCGTGACAGAACGCTTGCCCAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTg                                                                                                                                                <  1:76432/150‑1 (MQ=255)
      gTGCCAACACCTGTCCAGACTCCTGCAATTTACCGCGAACTTCATAAAACCAGTTAAGGATTGCAGGGGGTAATGGCGTGACAGAACGCTTGCCCAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTg                                                                                                                                                <  2:1175644/150‑1 (MQ=255)
       tGCCAACACCTGTCCAGACTCCTGCAATTTACCGCGAACTTCATAAAACCAGTTAAGGATTGCAGGGGGTAATGGCGTGACAGAACGCTTGCCCAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTTGCCACTGc                                                                                                                                               >  2:387143/1‑150 (MQ=255)
          cAACACCTGTCCAGACTCCTGCAATTTACCGCGAACTTCATAAAACCAGTTAAGGATTGCAGGGGGTAATGGCGTGACAGAACGCTTGCCCAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTTGCCACTGccct                                                                                                                                            <  1:114668/150‑3 (MQ=255)
          cAACACCTGTCCAGACTCCTGCAATTTACCGCGAACTTCATAAAACCAGTTAAGGATTGCAGGGGGTAATGGCGTGACAGAACGCTTGCCCAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCgg                                                                                                                                            >  1:897637/1‑150 (MQ=255)
           aaCACCTGTCCAGACTCCTGCAATTTACCGCGAACTTCATAAAACCAGTTAAGGATTGCAGGGGGTAATGGCGTGACAGAACGCTTGCCCAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTTGCCACTGccctg                                                                                                                                           <  1:15327/150‑4 (MQ=255)
              acCTGTCCAGACTCCTGCAATTTACCGCGAACTTCATAAAACCAGTTAAGGATTGCAGGGGGTAATGGCGTGACAGAACGCTTGCCCAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCa                                                                                                                                        <  1:1059302/150‑1 (MQ=255)
               ccTGTCCAGACTCCTGCAATTTACCGCGAACTTCATAAAACCAGTTAAGGATTGCAGGGGGTAATGGCGTGACAGAACGCTTGCCCAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCaa                                                                                                                                       >  1:802844/1‑150 (MQ=255)
                  gTCCAGACTCCTGCAATTTACCGCGAACTTCATAAAACCAGTTAAGGATTGCAGGGGGTAATGGCGTGACAGAACGCTTGCCCAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCt                                                                                                                                    <  1:833722/150‑1 (MQ=255)
                         cTCCTGCAATTTACCGCGAACTTCATAAAACCAGTTAAGGATTGCAGGGGGTAATGGCGTGACAGAACGCTTGCCCAGCCATCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGcc                                                                                                                             >  2:1590376/1‑150 (MQ=255)
                             tGCAATTTACCGCGAACTTCATAAAACCAGTTAAGGATTGCAGGGGGTAATGGCGTGACAGAACGCTTGCCCAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAgg                                                                                                                         <  2:1155182/150‑1 (MQ=255)
                              gCAATTTACTGCGAACTTCATAAAACCAGTTAAGGATTGCAGGGGGTAATGGCGTGACAGAACGCTTGCCCAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAggg                                                                                                                        >  2:1518035/1‑150 (MQ=255)
                              gCAATTTACCGCGAACTTCATAAAACCAGTTAAGGATTGCAGGGGGTAATGGCGTGACAGAACGCTTGCCCAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAggg                                                                                                                        >  2:376091/1‑150 (MQ=255)
                                aaTTTACCGCGAACTTCATAAAACCAGTTAAGGATTGCAGGGGGTAATGGCGTGACAGAACGCTTGCCCAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCa                                                                                                                      <  2:1240987/150‑1 (MQ=255)
                                     aCCGCGAACTTCATAAAACCAGTTAAGGATTGCAGGGGGTAATGGCGTGACAGAACGCTTGCCCAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTg                                                                                                                 >  1:1562553/1‑150 (MQ=255)
                                        gcgAACTTCATAAAACCAGTTAAGGATTGCAGGGGGTAATGGCGTGACAGAACGCTTGCCCAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCca                                                                                                              >  1:1431828/1‑150 (MQ=255)
                                        gcgAACTTCATAAAACCAGTTAAGGATTGCAGGGGGTAATGGCGTGACAGAACGCTTGCCCAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCca                                                                                                              <  2:261663/150‑1 (MQ=255)
                                        gcgAACTTCATAAAACCAGTTAAGGATTGCAGGGGGTAATGGCGTGACAGAACGCTTGCCCAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCca                                                                                                              <  2:45725/150‑1 (MQ=255)
                                          gAACTTCATAAAACCAGTTAAGGATTGCAGGGGGTAATGGCGTGACAGAACGCTTGCCCAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCcagc                                                                                                            >  2:1301606/1‑150 (MQ=255)
                                           aaCTTCATAAAACCAGTTAAGGATTGCAGGGGGTAATGGCGTGACAGAACGCTTGCCCAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGCCCGCCCAGGGCAATCTGCcagca                                                                                                           >  2:240758/1‑150 (MQ=255)
                                           aaCTTCATAAAACCAGTTAAGGATTGCAGGGGGTAATGGCGTGACAGAACGCTTGCCCAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCcagca                                                                                                           >  2:1356845/1‑150 (MQ=255)
                                               tCATAAAACCAGTTAAGGATTGCAGGGGGTAATGGCGTGACAGAACGCTTGCCCAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCagag                                                                                                        <  2:1528225/149‑1 (MQ=255)
                                                   aaaaCCAGTTAAGGATTGCAGGGGGTAATGGCGTGACAGAACGCTTGCCCAGCCACCACAATCCCTGCATGGGTAACATTAAGGCGAACAGGCAAGTGGCAAATCCCGCCCCAGCAGGCCCCCCCAGGCCAAAATGCCAGAAGAGATTaa                                                                                                   >  2:1255856/1‑150 (MQ=255)
                                                    aaaCCAGTTAAGGATTGCAGGGGGTAATGGCGTGACAGAACGCTTGCCCAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTaaa                                                                                                  >  1:1036581/1‑150 (MQ=255)
                                                      aCCAGTTAAGGATTGCAGGGGGTAATGGCGTGACAGAACGCTTGCCCAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATa                                                                                                <  1:1188132/150‑1 (MQ=255)
                                                       ccAGTTAAGGATTGCAGGGGGTAATGGCGTGACAGAACGCTTGCCCAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATAc                                                                                               >  1:973702/1‑150 (MQ=255)
                                                        cAGTTAAGGATTGCAGGGGGTAATGGCGTGACAGAACGCTTGCCCAGCCACCACAATCCCTGCATGGGTTAACTTAAGGCGAGCAGGGCTGTGGCTACTGCCGGCCCCAGCGGACCGCGCAGGGCAATCTGCCAGCAGAGAGTAAATACg                                                                                              >  2:111766/1‑150 (MQ=255)
                                                          gTTAAGGATTGCAGGGGGTAATGGCGTGACAGAACGCTTGCCCAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGc                                                                                            <  1:64143/150‑1 (MQ=255)
                                                          gTTAAGGATTGCAGGGGGTAATGGCGTGACAGAACGCTTGCCCAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGc                                                                                            >  1:954145/1‑150 (MQ=255)
                                                          gTTAAGGATTGCAGGGGGTAATGGCGTGACAGAACGCTTGCCCAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGc                                                                                            <  1:118454/150‑1 (MQ=255)
                                                          gTTAAGGATTGCAGGGGGTAATGGCGTGACAGAACGCTTGCCCAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGc                                                                                            <  2:1001086/150‑1 (MQ=255)
                                                            tAAGGATTGCAGGGGGTAATGGCGTGACAGAACGCTTGCCCAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGa                                                                                          >  1:7168/1‑150 (MQ=255)
                                                                  ttGCAGGGGGTAATGGCGTGACAGAACGCTTGCCCAGCCACCACAATCCGTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATcggcg                                                                                    <  2:1445194/150‑1 (MQ=255)
                                                                    gCAGGGGGTTATTGCGTGTCAGAAAGCTTGGCCATCCAACACAATCCCTTCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCggcggc                                                                                  <  1:1255856/150‑1 (MQ=255)
                                                                    gCAGGGGGTAATGGCGTGACAGAACGCTTGCCCAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCggcggc                                                                                  >  2:1481298/1‑150 (MQ=255)
                                                                     cAGGGGGTAATGGCGTGACAGAACGCTTGCCCAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCa                                                                                 <  2:1283608/150‑1 (MQ=255)
                                                                       gggggTAATGGCGTGACAGAACGCTTGCCCAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATa                                                                               >  2:1522716/1‑150 (MQ=255)
                                                                         gggTAATGGCGTGACAGAACGCTTGCCCAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATaaa                                                                             >  2:1660007/1‑150 (MQ=255)
                                                                          ggTAATGGCGTGACAGAACGCTTGCCCAGCCACCACAATCCCTGCATGGGTAACCTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCCAGCTGACCGGCCAGGGCAATCTGCCAGCAGAGAGTAAAAACGGCGATCGGCGGCATaaaa                                                                            >  2:126111/1‑150 (MQ=255)
                                                                           gTAATGGCGTGACAGAACGCTTGCCCAGCCATCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAAc                                                                           <  1:1590376/150‑1 (MQ=255)
                                                                           gTAATGGCGTGACAGAACGCTTGCCCAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAAc                                                                           <  2:973702/150‑1 (MQ=255)
                                                                            tAATGGCGTAACAGAACGCTTGCCCAGCCACCACAATCCCTGCATGGGTAAACTTAAGGTGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACg                                                                          <  1:1345553/150‑1 (MQ=255)
                                                                            tAATGGCGGGACAGAACGCTTGCCCAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACg                                                                          <  1:1498257/150‑1 (MQ=255)
                                                                                  cGTGACAGAACGCTTGCCCAGCCACCACAATCCCTGCTTGGGTAAACTTAACGCGAACCGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGc                                                                    <  1:451065/150‑1 (MQ=255)
                                                                                    tGACAGAACGCTTGCCCAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCaa                                                                  >  2:485338/1‑149 (MQ=255)
                                                                                       cAGAACGCTTGCCCAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAAc                                                               >  2:1367800/1‑150 (MQ=255)
                                                                                        aGAACGCTTGCCCAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCACTCTGCCCGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCAAAAcc                                                              >  2:78385/1‑149 (MQ=255)
                                                                                        aGAACGCTTGCCCAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAAcg                                                              <  1:362089/150‑1 (MQ=255)
                                                                                         gAACGCTTGCCCAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGCCAACTGCCGGCCCAAGCTGACCGCCCATGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAAcgc                                                             >  1:723448/1‑150 (MQ=255)
                                                                                           aCGCTTGCCCAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGt                                                           <  2:118923/150‑1 (MQ=255)
                                                                                            cGCTTGCCCAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTc                                                          >  1:575921/1‑150 (MQ=255)
                                                                                             gCTTGCCCAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCa                                                         <  2:1698801/150‑1 (MQ=255)
                                                                                              cTTGCCCAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCAt                                                        <  2:1500413/150‑1 (MQ=255)
                                                                                              cTTGCCCAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCAt                                                        <  2:1561809/150‑1 (MQ=255)
                                                                                               ttGCCCAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATc                                                       <  1:1091362/150‑1 (MQ=255)
                                                                                                tGCCCAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCt                                                      >  1:127056/1‑150 (MQ=255)
                                                                                                 gCCCAGCCACCACAATCCCTGAATGGGTAAACTTAAGGCGAACAGGTCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCtt                                                     <  2:333237/150‑1 (MQ=255)
                                                                                                  cccAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTg                                                    <  1:774872/150‑1 (MQ=255)
                                                                                                  cccAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTg                                                    >  1:803047/1‑150 (MQ=255)
                                                                                                      gCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGCCCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATaa                                                >  2:491699/1‑150 (MQ=255)
                                                                                                       ccaccaCAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATAGCATAACGCGTCATCTTGATAAc                                               >  1:876919/1‑150 (MQ=255)
                                                                                                          ccacAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAAcgcg                                            <  1:856997/150‑1 (MQ=255)
                                                                                                           cacaATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGa                                           >  1:291524/1‑150 (MQ=255)
                                                                                                               aTCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGAtttt                                       <  1:1334982/150‑1 (MQ=255)
                                                                                                                tCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGAttttg                                      >  2:1675796/1‑149 (MQ=255)
                                                                                                                tCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTc                                      <  2:1630680/150‑1 (MQ=255)
                                                                                                                 cccTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGGAACTGCCGGCCCACGCGGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCg                                     <  2:1095682/150‑1 (MQ=255)
                                                                                                                  ccTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGa                                    <  1:1481298/150‑1 (MQ=255)
                                                                                                                   cTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCAAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGAc                                   >  1:244161/1‑150 (MQ=255)
                                                                                                                       aTGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAg                               <  2:781676/150‑1 (MQ=255)
                                                                                                                        tGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGCTCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGa                              >  1:152900/1‑150 (MQ=255)
                                                                                                                        tGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGa                              >  2:1266289/1‑150 (MQ=255)
                                                                                                                        tGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGAAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCAGCTTGATAACGCGATTTTCGACAAAGa                              >  2:1413187/1‑150 (MQ=255)
                                                                                                                         gggTACACTTAAGTCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCAGCCAGCAGAGAGTAAATACGGCGATCGGAGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGAc                             <  1:293190/150‑1 (MQ=255)
                                                                                                                           gTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCg                           <  2:575921/150‑1 (MQ=255)
                                                                                                                             aaaCTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCggg                         <  2:1385524/150‑1 (MQ=255)
                                                                                                                              aaCTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCgggg                        >  1:1505367/1‑150 (MQ=255)
                                                                                                                                cTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCa                      <  2:1036581/150‑1 (MQ=255)
                                                                                                                                cTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCa                      >  1:703177/1‑150 (MQ=255)
                                                                                                                                   aaGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAgg                   <  1:789477/150‑1 (MQ=255)
                                                                                                                                     ggCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCg                 <  1:1660007/150‑1 (MQ=255)
                                                                                                                                         aaCAGGGCATTGGCAACTGCCGGCCCAAGCTGACCGCCCATGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGtttt             <  2:723448/150‑1 (MQ=255)
                                                                                                                                          aCAGGGCAGTGTCAACTGCCGGCCCAAGCTGACCGCCCATGGAAATCTGCCAGCAGAGAGAAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGttttt            <  1:88916/150‑1 (MQ=255)
                                                                                                                                            ccggCAGTTGCCACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTcc          >  1:1449200/3‑150 (MQ=255)
                                                                                                                                            aGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGAGTTTCGACAAAGACCGGGGCAAGGCGTTTTTcc          >  1:1563058/1‑150 (MQ=255)
                                                                                                                                             gggCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCa         <  2:291524/150‑1 (MQ=255)
                                                                                                                                              ggCAGTTGCCACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCTTCATCTTGATAACGCGTTTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAg        >  2:768267/1‑150 (MQ=255)
                                                                                                                                              ggCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAg        <  1:603783/150‑1 (MQ=255)
                                                                                                                                              ggCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAg        <  1:1518035/150‑1 (MQ=255)
                                                                                                                                               gCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGc       >  1:1356652/1‑150 (MQ=255)
                                                                                                                                               gCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGc       <  2:802844/150‑1 (MQ=255)
                                                                                                                                                cAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCg      <  1:475891/150‑1 (MQ=255)
                                                                                                                                                 aGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCgg     <  2:917128/150‑1 (MQ=255)
                                                                                                                                                   tGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGcc   <  1:1554878/150‑1 (MQ=255)
                                                                                                                                                   tGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGACAGCAGAGAGTAAATAAGGCGAACGGCGGCATAAAACGGATAGCATAACGCGACATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGGTTTGACAGCggac   >  2:669348/1‑148 (MQ=255)
                                                                                                                                                    ggCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCa  >  2:1270901/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CAACGGGTGCCAACACCTGTCCAGACTCCTGCAATTTACCGCGAACTTCATAAAACCAGTTAAGGATTGCAGGGGGTAATGGCGTGACAGAACGCTTGCCCAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCA  >  NC_000913/2412763‑2413060

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: