Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,412,914 A→C 5.1% V73V (GTT→GTG yfbV ← UPF0208 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,412,9140AC 5.1% 430.9 / 10.0 138V73V (GTT→GTGyfbVUPF0208 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (71/59);  new base C (3/4);  total (75/63)
Fisher's exact test for biased strand distribution p-value = 7.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.76e-01

Only 100 of 140 total aligned reads displayed.

CGGGTGCCAACACCTGTCCAGACTCCTGCAATTTACCGCGAACTTCATAAAACCAGTTAAGGATTGCAGGGGGTAATGGCGTGACAGAACGCTTGCCCAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGT  >  NC_000913/2412766‑2413063
                                                                                                                                                    |                                                                                                                                                     
cGGGTGCCAACACCTGTCCAGACTCCTGCAATTTACCGCGAACTTCATAAAACCAGTTAAGGATTGCAGGGGGTAATGGCGTGACAGAACGCTTGCCCAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCaa                                                                                                                                                      >  1:1385524/1‑150 (MQ=255)
   gTGCCAACACCTGTCCAGACTCCTGCAATTTACCGCGAACTTCATAAAACCAGTTAAGGATTGCAGGGGGTAATGGCGTGACAGAACGCTTGCCCAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTg                                                                                                                                                   <  1:76432/150‑1 (MQ=255)
   gTGCCAACACCTGTCCAGACTCCTGCAATTTACCGCGAACTTCATAAAACCAGTTAAGGATTGCAGGGGGTAATGGCGTGACAGAACGCTTGCCCAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTg                                                                                                                                                   <  2:1175644/150‑1 (MQ=255)
    tGCCAACACCTGTCCAGACTCCTGCAATTTACCGCGAACTTCATAAAACCAGTTAAGGATTGCAGGGGGTAATGGCGTGACAGAACGCTTGCCCAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTTGCCACTGc                                                                                                                                                  >  2:387143/1‑150 (MQ=255)
     gCCAACACCTGTCCAGACTCCTGCAATTTACAGCGAACTTCATAAAACCAGTTAAGGATTGCAGGGGGTAATGGCGTGACAGAACGCTTGCCCAGCCCCCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGcc                                                                                                                                                 >  1:829042/1‑150 (MQ=255)
       cAACACCTGTCCAGACTCCTGCAATTTACCGCGAACTTCATAAAACCAGTTAAGGATTGCAGGGGGTAATGGCGTGACAGAACGCTTGCCCAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCgg                                                                                                                                               >  1:897637/1‑150 (MQ=255)
        aaCACCTGTCCAGACTCCTGCAATTTACCGCGAACTTCATAAAACCAGTTAAGGATTGCAGGGGGTAATGGCGTGACAGAACGCTTGCCCAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTTGCCACTGccctg                                                                                                                                              <  1:15327/150‑4 (MQ=255)
          cacCTGTCCTGACTCCTGCAATTTACCGCGAACTTCATAAAACCAGTTAAGGATTGCAGGGGGTAATGGCGTGACAGAACGCTTGCCCAGCCACCACAATCCCTGCAGGGGTAAACTTAAGGCGAACAGGGCAGTCGCAACTGCCGGccc                                                                                                                                            >  2:285012/1‑150 (MQ=255)
           acCTGTCCAGACTCCTGCAATTTACCGCGAACTTCATAAAACCAGTTAAGGATTGCAGGGGGTAATGGCGTGACAGAACGCTTGCCCAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCa                                                                                                                                           <  1:1059302/150‑1 (MQ=255)
               gTCCAGACTCCTGCAATTTACCGCGAACTTCATAAAACCAGTTAAGGATTGCAGGGGGTAATGGCGTGACAGAACGCTTGCCCAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCt                                                                                                                                       <  1:833722/150‑1 (MQ=255)
                  cAGACTCCTGCAATTTACCGCGAACTTCATAAAACCAGTTAAGGATTGCAGGGGGTAATGGCGTGACAGAACGCTTGCCCAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGAc                                                                                                                                    >  1:1630680/1‑150 (MQ=255)
                      cTCCTGCAATTTACCGCGAACTTCATAAAACCAGTTAAGGATTGCAGGGGGTAATGGCGTGACAGAACGCTTGCCCAGCCATCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGcc                                                                                                                                >  2:1590376/1‑150 (MQ=255)
                         cTGCAATTTACCGCGAACTTCATAAAACCAGTTAAGGATTGCAGGGGGTAATGGCGTGACAGAACGCTTGCCCAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAg                                                                                                                             >  2:1356438/1‑150 (MQ=255)
                          tGCAATTTACCGCGAACTTCATAAAACCAGTTAAGGATTGCAGGGGGTAATGGCGTGACAGAACGCTTGCCCAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAgg                                                                                                                            <  2:1155182/150‑1 (MQ=255)
                          tGCAATTTACCGCGAACTTCATAAAACCAGTTAAGGATTGCAGGGGGTAATGGCGTGACAGAACGCTTGCCCAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAgg                                                                                                                            >  1:1561809/1‑150 (MQ=255)
                           gCAATTTACTGCGAACTTCATAAAACCAGTTAAGGATTGCAGGGGGTAATGGCGTGACAGAACGCTTGCCCAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAggg                                                                                                                           >  2:1518035/1‑150 (MQ=255)
                           gCAATTTACCGCGAACTTCATAAAACCAGTTAAGGATTGCAGGGGGTAATGGCGTGACAGAACGCTTGCCCAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAggg                                                                                                                           >  2:376091/1‑150 (MQ=255)
                           gCAATTTACCGCGAACTTCATAAAACCAGTTAAGGATTGCAGGGGGTAATGGCGTGACAGAACGCTTGCCCAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAggg                                                                                                                           >  2:7752/1‑150 (MQ=255)
                           gCAATTTACCGCGAACTTCATAAAACCAGTTAAGGATTGCAGGGGGTAATGGCGTGACAGAACGCTTGCCCAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGAACGCCCAggg                                                                                                                           >  2:1695782/1‑150 (MQ=255)
                             aaTTTACCGCGAACTTCATAAAACCAGTTAAGGATTGCAGGGGGTAATGGCGTGACAGAACGCTTGCCCAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCa                                                                                                                         <  2:1240987/150‑1 (MQ=255)
                                  aCCGCGAACTTCATAAAACCAGTTAAGGATTGCAGGGGGTAATGGCGTGACAGAACGCTTGCCCAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTg                                                                                                                    >  1:1562553/1‑150 (MQ=255)
                                     gcgAACTTCATAAAACCAGTTAAGGATTGCAGGGGGTAATGGCGTGACAGAACGCTTGCCCAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCca                                                                                                                 >  1:1431828/1‑150 (MQ=255)
                                       gAACTTCATAAAACCAGTTAAGGATTGCAGGGGGTAATGGCGTGACAGAACGCTTGCCCAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCcagc                                                                                                               >  2:1301606/1‑150 (MQ=255)
                                        aaCTTCATAAAACCAGTTAAGGATTGCAGGGGGTAATGGCGTGACAGAACGCTTGCCCAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGCCCGCCCAGGGCAATCTGCcagca                                                                                                              >  2:240758/1‑150 (MQ=255)
                                        aaCTTCATAAAACCAGTTAAGGATTGCAGGGGGTAATGGCGTGACAGAACGCTTGCCCAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCcagca                                                                                                              >  2:1356845/1‑150 (MQ=255)
                                            tCATAAAACCAGTTAAGGATTGCAGGGGGTAATGGCGTGACAGAACGCTTGCCCAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCagag                                                                                                           >  1:529815/1‑149 (MQ=255)
                                            tCATAAAACCAGTTAAGGATTGCAGGGGGTAATGGCGTGACAGAACGCTTGCCCAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCagag                                                                                                           <  2:529815/149‑1 (MQ=255)
                                                aaaaCCAGTTAAGGATTGCAGGGGGTAATGGCGTGACAGAACGCTTGCCCAGCCACCACAATCCCTGCATGGGTAACATTAAGGCGAACAGGCAAGTGGCAAATCCCGCCCCAGCAGGCCCCCCCAGGCCAAAATGCCAGAAGAGATTaa                                                                                                      >  2:1255856/1‑150 (MQ=255)
                                                 aaaCCAGTTAAGGATTGCAGGGGGTAATGGCGTGACAGAACGCTTGCCCAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTaaa                                                                                                     >  1:1036581/1‑150 (MQ=255)
                                                   aCCAGTTAAGGATTGCAGGGGGTAATGGCGTGACAGAACGCTTGCCCAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATa                                                                                                   >  1:1500413/1‑150 (MQ=255)
                                                    ccAGTTAAGGATTGCAGGGGGTAATGGCGTGACAGAACGCTTGCCCAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATAc                                                                                                  >  1:973702/1‑150 (MQ=255)
                                                    ccAGTTAAGGATTGCAGGGGGTAATGGCGTGACAGAACGCTTGCCCAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATAc                                                                                                  <  1:86080/150‑1 (MQ=255)
                                                     cAGTTAAGGATTGCAGGGGGTAATGGCGTGACAGAACGCTTGCCCAGCCACCACAATCCCTGCATGGGTTAACTTAAGGCGAGCAGGGCTGTGGCTACTGCCGGCCCCAGCGGACCGCGCAGGGCAATCTGCCAGCAGAGAGTAAATACg                                                                                                 >  2:111766/1‑150 (MQ=255)
                                                       gTTAAGGATTGCAGGGGGTAATGGCGTGACAGAACGCTTGCCCAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGc                                                                                               >  1:954145/1‑150 (MQ=255)
                                                       gTTAAGGATTGCAGGGGGTAATGGCGTGACAGAACGCTTGCCCAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGc                                                                                               <  2:1001086/150‑1 (MQ=255)
                                                       gTTAAGGATTGCAGGGGGTAATGGCGTGACAGAACGCTTGCCCAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGc                                                                                               <  1:118454/150‑1 (MQ=255)
                                                                 gCAGGGGGTTATTGCGTGTCAGAAAGCTTGGCCATCCAACACAATCCCTTCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCggcggc                                                                                     <  1:1255856/150‑1 (MQ=255)
                                                                 gCAGGGGGTAATGGCGTGACAGAACGCTTGCCCAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCggcggc                                                                                     >  2:1481298/1‑150 (MQ=255)
                                                                  cAGGGGGTAATGGCGTGACAGAACGCTTGCCCAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCa                                                                                    <  2:1283608/150‑1 (MQ=255)
                                                                    gggggTAATGGCGTGACAGAACGCTTGCCCAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATa                                                                                  >  2:1522716/1‑150 (MQ=255)
                                                                      gggTAATGGCGTGACAGAACGCTTGCCCAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATaaa                                                                                >  2:1660007/1‑150 (MQ=255)
                                                                       ggTAATGGCGTGACAGAACGCTTGCCCAGCCACCACAATCCCTGCATGGGTAACCTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCCAGCTGACCGGCCAGGGCAATCTGCCAGCAGAGAGTAAAAACGGCGATCGGCGGCATaaaa                                                                               >  2:126111/1‑150 (MQ=255)
                                                                        gTAATGGCGTGACAGAACGCTTGCCCAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAAc                                                                              <  2:973702/150‑1 (MQ=255)
                                                                         tAATGGCGTGACAGAACGCTTGCCCAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACg                                                                             >  1:781676/1‑150 (MQ=255)
                                                                         tAATGGCGTAACAGAACGCTTGCCCAGCCACCACAATCCCTGCATGGGTAAACTTAAGGTGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACg                                                                             <  1:1345553/150‑1 (MQ=255)
                                                                               cGTGACAGAACGCTTGCCCAGCCACCACAATCCCTGCTTGGGTAAACTTAACGCGAACCGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGc                                                                       <  1:451065/150‑1 (MQ=255)
                                                                                 tGACAGAACGCTTGCCCAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCaa                                                                     >  2:485338/1‑149 (MQ=255)
                                                                                    cAGAACGCTTGCCCAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAAc                                                                  >  2:1367800/1‑150 (MQ=255)
                                                                                     aGAACGCTTGCCCAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCACTCTGCCCGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCAAAAcc                                                                 >  2:78385/1‑149 (MQ=255)
                                                                                      gAACGCTTGCCCAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGCCAACTGCCGGCCCAAGCTGACCGCCCATGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAAcgc                                                                >  1:723448/1‑150 (MQ=255)
                                                                                        aCGCTTGCCCAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGt                                                              <  2:118923/150‑1 (MQ=255)
                                                                                         cGCTTGCCCAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTc                                                             >  1:575921/1‑150 (MQ=255)
                                                                                          gCTTGCCCAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCa                                                            <  2:1698801/150‑1 (MQ=255)
                                                                                          gCTTGCCCAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCa                                                            <  1:1356845/150‑1 (MQ=255)
                                                                                           cTTGCCCAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCAt                                                           <  2:1561809/150‑1 (MQ=255)
                                                                                           cTTGCCCAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCAt                                                           <  2:1500413/150‑1 (MQ=255)
                                                                                            ttGCCCAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATc                                                          <  1:1091362/150‑1 (MQ=255)
                                                                                             tGCCCAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCt                                                         >  1:127056/1‑150 (MQ=255)
                                                                                               cccAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTg                                                       >  2:475891/1‑150 (MQ=255)
                                                                                               cccAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTg                                                       >  1:803047/1‑150 (MQ=255)
                                                                                                    ccaccaCAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATAGCATAACGCGTCATCTTGATAAc                                                  >  1:876919/1‑150 (MQ=255)
                                                                                                       ccacAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAAcgcg                                               <  1:856997/150‑1 (MQ=255)
                                                                                                        cacaATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGa                                              >  1:291524/1‑150 (MQ=255)
                                                                                                            aTCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGAtttt                                          <  1:1334982/150‑1 (MQ=255)
                                                                                                             tCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTc                                         <  2:1630680/150‑1 (MQ=255)
                                                                                                              cccTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGGAACTGCCGGCCCACGCGGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCg                                        <  2:1095682/150‑1 (MQ=255)
                                                                                                               ccTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGa                                       <  1:1481298/150‑1 (MQ=255)
                                                                                                                cTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGTGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGAGTTTCGACa                                     >  2:375821/1‑150 (MQ=255)
                                                                                                                   cATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACaaa                                   >  1:1249712/1‑150 (MQ=255)
                                                                                                                    aTGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAg                                  <  2:781676/150‑1 (MQ=255)
                                                                                                                     tGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGCTCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGa                                 >  1:152900/1‑150 (MQ=255)
                                                                                                                     tGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGa                                 >  2:1266289/1‑150 (MQ=255)
                                                                                                                     tGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGAAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCAGCTTGATAACGCGATTTTCGACAAAGa                                 >  2:1413187/1‑150 (MQ=255)
                                                                                                                      gggTACACTTAAGTCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCAGCCAGCAGAGAGTAAATACGGCGATCGGAGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGAc                                <  1:293190/150‑1 (MQ=255)
                                                                                                                       ggTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGTCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGGCATCTTGATAACGCGATTTTCGACAAAGccc                               >  2:1472522/1‑150 (MQ=255)
                                                                                                                       ggTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGAcc                               >  2:764616/1‑150 (MQ=255)
                                                                                                                        gTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCg                              <  2:575921/150‑1 (MQ=255)
                                                                                                                          aaaCTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCggg                            <  2:1385524/150‑1 (MQ=255)
                                                                                                                          aaaCTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCCGCGGCATAAAACGGATCGCATAACGCGTCATCTTTATAACGCGATTTTCGACAAAGACCggg                            >  1:398907/1‑150 (MQ=255)
                                                                                                                           aaCTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCgggg                           >  1:1505367/1‑150 (MQ=255)
                                                                                                                             cTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGGCCGCCCAGGGCCATCTGCCAGCTGAGAGTAACTACGGCGATCGGCGGCATATCACGGATCGCATAACGCGTCATCTTGATACCGCGCTTTTCGACAAAGACCGGGGCa                         >  1:273801/1‑150 (MQ=255)
                                                                                                                             cTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCa                         <  2:1036581/150‑1 (MQ=255)
                                                                                                                             cTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCa                         >  1:703177/1‑150 (MQ=255)
                                                                                                                                aaGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAgg                      <  1:789477/150‑1 (MQ=255)
                                                                                                                                  ggCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCg                    <  1:1660007/150‑1 (MQ=255)
                                                                                                                                   gCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGt                   >  2:1558202/1‑150 (MQ=255)
                                                                                                                                       aCAGGGCAGTGTCAACTGCCGGCCCAAGCTGACCGCCCATGGAAATCTGCCAGCAGAGAGAAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGttttt               <  1:88916/150‑1 (MQ=255)
                                                                                                                                        cAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCGTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTc              >  1:717748/1‑150 (MQ=255)
                                                                                                                                         ccggCAGTTGCCACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTcc             >  1:1449200/3‑150 (MQ=255)
                                                                                                                                         aGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGAGTTTCGACAAAGACCGGGGCAAGGCGTTTTTcc             >  1:1563058/1‑150 (MQ=255)
                                                                                                                                          gggCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCATGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAAAGGATCGCATACCGCGTCATCTTGATAACGCGAGTTTCGCCAAAGACCGGGGCAAGGCGTTTTTCCa            >  1:684909/1‑150 (MQ=255)
                                                                                                                                          gggCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCa            <  2:291524/150‑1 (MQ=255)
                                                                                                                                           ggCAGTTGCCACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCTTCATCTTGATAACGCGTTTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAg           >  2:768267/1‑150 (MQ=255)
                                                                                                                                           ggCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAg           <  1:1518035/150‑1 (MQ=255)
                                                                                                                                           ggCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAg           <  1:603783/150‑1 (MQ=255)
                                                                                                                                              aGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCgg        <  2:917128/150‑1 (MQ=255)
                                                                                                                                                tGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGACAGCAGAGAGTAAATAAGGCGAACGGCGGCATAAAACGGATAGCATAACGCGACATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGGTTTGACAGCggac      >  2:669348/1‑148 (MQ=255)
                                                                                                                                                 ggCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCa     >  2:1270901/1‑150 (MQ=255)
                                                                                                                                                  gCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGAGAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGcccc    >  2:1037669/1‑148 (MQ=255)
                                                                                                                                                   cAACCGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCGTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACg   >  1:1148814/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CGGGTGCCAACACCTGTCCAGACTCCTGCAATTTACCGCGAACTTCATAAAACCAGTTAAGGATTGCAGGGGGTAATGGCGTGACAGAACGCTTGCCCAGCCACCACAATCCCTGCATGGGTAAACTTAAGGCGAACAGGGCAGTGGCAACTGCCGGCCCAAGCTGACCGCCCAGGGCAATCTGCCAGCAGAGAGTAAATACGGCGATCGGCGGCATAAAACGGATCGCATAACGCGTCATCTTGATAACGCGATTTTCGACAAAGACCGGGGCAAGGCGTTTTTCCAGCGGCCACGT  >  NC_000913/2412766‑2413063

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: