Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 212,730 C→A 5.4% P134T (CCT→ACT)  tilS → tRNA(Ile)‑lysidine synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913212,7300CA 5.4% 385.1 / 7.9 120P134T (CCT→ACT) tilStRNA(Ile)‑lysidine synthetase
Reads supporting (aligned to +/- strand):  ref base C (59/54);  new base A (3/4);  total (62/58)
Fisher's exact test for biased strand distribution p-value = 7.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.96e-01

Only 100 of 123 total aligned reads displayed.

AAGAAGGACTGGGCATTGAGGCCCAGGCGCGGCAGGCACGTTATCAGGCATTTGCCCGCACCTTGTTGCCCGGTGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCCGCACGCGGGGGGAACTGGTGCAGTGGGCGCGTCAGTATGATTTACGCTGGATTGAAGACGAAAGTAATCAGGACGAC  >  NC_000913/212581‑212879
                                                                                                                                                     |                                                                                                                                                     
aagaagGACTGGGCATTGAGGCCCAGGCGCGGCAGGCACGTTATCAGGCATTTGCCCGCCCCTTGTTGCCCGGTGAAGTGCTGGTCACCGTGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGcc                                                                                                                                                       >  2:756341/1‑150 (MQ=255)
    agGACTGGGCATTGAGGCCCAGGCGCGGTAGGCACGTTATCAGGCATTTGCCCGCACCTTGTTGCCCGGTGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGc                                                                                                                                                   <  2:435447/150‑1 (MQ=255)
    agGACTGGGCATTGAGGCCCAGGCGCGGCAGGCACGTTATCAGGCATTTGCCCGCACCTTGTTGCCCGGTGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGc                                                                                                                                                   >  1:254932/1‑150 (MQ=255)
     ggACTGGGCATTGAGGCCCAGGCGCGGCAGGCACGTTATCAGGCATTTGCCCGCACCTTGTTGCCCGGTGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGcc                                                                                                                                                  >  1:557110/1‑150 (MQ=255)
      gACTGGGCATTGAGGCCCAGGCGCGGCAGGCACGTTATCAGGCATTTGCCCGCACCTTGTTGCCCGGTGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCg                                                                                                                                                 >  2:23209/1‑150 (MQ=255)
       aCTGGGCATTGAGGCCCAGGCGCGGCAGGCACGTTATCAGGCATTTGCCCGCACCTTGTTGCCCGGTGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCgg                                                                                                                                                <  1:526665/150‑1 (MQ=255)
       aCTGGGCATTGAGGCCCAGGCGCGGCAGGCACGTTATCAGGCATTTGCCCGCACCTTGTTGCCCGGTGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCgg                                                                                                                                                >  1:1463364/1‑150 (MQ=255)
        cTGGGCATTGCGGCCCAGGCGCGGCAGGCACGTTATCAGGCATTTGCCCGCACCTTGGTGCCCGGTGAAGGGCGGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCggg                                                                                                                                               <  2:1489983/150‑1 (MQ=255)
        cTGGGCATTGCGGCCCAGGCGCGGCAGGCACGTTATCAGCCAGTTGCCCGCACCTAGTAGCCCGGTGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTCAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCggg                                                                                                                                               <  2:100262/150‑1 (MQ=255)
        cTGGGCATTGAGGCCCAGGCGCGGCAGGCACGTTATCAGGCATTTGCCCGCACCTTGTTGCCCGGTGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCggg                                                                                                                                               <  2:392351/150‑1 (MQ=255)
        cTGGGCATTGAGGCCCAGGCGCGGCAGGCACGTTATCAGGCATTTGCCCGCACCTTGTTGCCCGGTGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCggg                                                                                                                                               <  1:1344319/150‑1 (MQ=255)
        cTGGGCATTGAGGCCCAGGCGCGGCAGGCACGTTATCAGGCATTTGCCCGCACCTTGTTGCCCGGTGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCggg                                                                                                                                               <  1:1533514/150‑1 (MQ=255)
        cTGGGCATTGAGGCCCAGGCGCGGCAGGCACGTTATCAGGCATTTGCCCGCACCTTGTTGCCCGGTGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGGGCCACTGCCgcg                                                                                                                                               <  2:404701/150‑3 (MQ=255)
          gggCATTGAGGCCCAGGCGCGGCAGGCACGTTATCAGGCATTTGCCCGCACCTTGTTGCCCGGTGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTGAAACGCGGCAGTGGCCCTGCCGGGCt                                                                                                                                             >  1:1553801/1‑150 (MQ=255)
          gggCATTGAGGCCCAGGCGCGGCAGGCACGTTATCAGGCATTTGCCCGCACCTTGTTGCCCGGTGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGGGCCACTGCCgcgtt                                                                                                                                             <  1:1562027/150‑5 (MQ=255)
          gggCATTGAGGCCCAGGCGCGGCAGGCACGTTATCAGGCATTTGCCCGCACCTTGTTGCCCGGTGAAGTGCTGGGCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGGGCCACTGCCgcgtt                                                                                                                                             <  1:1442516/150‑5 (MQ=255)
           ggCATTGAGGCCCAGGCGCGGCAGGCACGTTATCAGGCATTTGCCCGCACCTTGTTGCCCGGTGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCtt                                                                                                                                            <  2:1541962/150‑1 (MQ=255)
           ggCATTGAGGCCCAGGCGCGGCAGGCACGTTATCAGGCATTTGCCCGCACCTTGTTGCCCGGGGAAGTGCTGGGCACCGCGCAAAATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCtt                                                                                                                                            <  2:1339924/150‑1 (MQ=255)
                tGAGGCCCAGGCGCGGCAGGCACGTTATCAGGCATTTGCCCGCACCTTGTTGCCCGGGGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGGGCCACTGCCGCGttttagcg                                                                                                                                       <  1:1482008/150‑5 (MQ=255)
                  aGGCCCAGGCGCGGCAGGCACGTTATCAGGCATTTGCCCGCACCTTGTTGCCCGGTGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTa                                                                                                                                     <  2:278849/150‑1 (MQ=255)
                   ggCCCAGGCGCGGCAGGCACGTTATCAGGCATTTGCCCGCACCTTGTTGCCCGGTGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTAt                                                                                                                                    >  1:468421/1‑150 (MQ=255)
                    gCCCAGGCGCGGCAGGCACGTTATCAGGCATTTGCCCGCACCTTGTTGCCCGGTGAAGTGCTGGTCACCGCGCAACATCTCGCCGATCAATGTGACACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATg                                                                                                                                   >  1:302281/1‑150 (MQ=255)
                         ggCGCGGCAGGCACGTTATCAGGCATTTGCCCGCACCTTGTTGCCCGGTGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGa                                                                                                                              >  2:927113/1‑150 (MQ=255)
                          gcgcGGCAGGCACGTTATCAGGCATTTGCCCGCACCTTGTTGCCCGGTGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGaa                                                                                                                             <  2:1020129/150‑1 (MQ=255)
                             cgGCAGGCACGTTATCAGGCATTTGCCCGCACCTTGTTGCCCGGTGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGtc                                                                                                                          <  2:940217/150‑1 (MQ=255)
                               gcaggcaCGTTATCAGGCATTTGCCCGCACCTTGTTGCCCGGTGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGtctc                                                                                                                        >  2:1718720/1‑150 (MQ=255)
                                  ggcaCGTTATCAGGCATTTGCCCGCACCTTGTTGCCCGGTGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGa                                                                                                                     >  1:803540/1‑150 (MQ=255)
                                    caCGTTATCAGGCATTTGCCCGCACCTTGTTGCCCGGTGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGt                                                                                                                   >  1:963637/1‑150 (MQ=255)
                                     aCGTTATCAGGCATTTGCCCGCACCTTGTTGCCCGGTGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGtt                                                                                                                  >  2:138891/1‑150 (MQ=255)
                                     aCGTTATCAGGCATTTGCCCGCACCTTGTTGCCCGGTGAAGTGCTGGTCACCGCGCAACATCTAGACGATCAATGTGAAACCTTTCTGCTGGCGCGAAAACGCGGCAGTGGACCTGCCGGGCTTTCGGGTATTGAGTAAGTCTCGGAGtt                                                                                                                  >  1:1045448/1‑150 (MQ=255)
                                      cGTTATCAGGCATTTGCCCGCACCTTGTTGCCCTGTGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAACCGCGGCCGTGGCCCTGCCGGGCTTTCGGCTATGGCTGAAGTCTCGGAGttt                                                                                                                 >  1:81216/1‑150 (MQ=255)
                                      cGTTATCAGGCATTTGCCCGCACCTTGTTGCCCGGTGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGttt                                                                                                                 <  2:409840/150‑1 (MQ=255)
                                      cGTTATCAGGCATTTGCCCGCACCTTGTTGCCCGGTGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGttt                                                                                                                 >  1:729445/1‑150 (MQ=255)
                                       gTTATCAGGCATTTGCCCGCACCTTGTTGCCCGGTGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTg                                                                                                                <  2:1207241/150‑1 (MQ=255)
                                       gTTATCAGGCATTTGCCCGCACCTTGTTGCCCGGTGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTg                                                                                                                <  2:646368/150‑1 (MQ=255)
                                          aTCAGGCATTTGCCCGCACCTTGTTGCCCGGTGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCg                                                                                                             <  1:988774/150‑1 (MQ=255)
                                          aTCAGGCATTTGCCCGCACCTTGTTGCCCGGCGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCg                                                                                                             >  1:1626453/1‑150 (MQ=255)
                                             aGGCATTTGCCCGCACCTTGTTGCCCGGTGAAGTGCTGGTCACCGCGAAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCAGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGaa                                                                                                          >  1:1047469/1‑150 (MQ=255)
                                               gCATTTGCCCGCACCTTGTTGCCCGGTGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAAcg                                                                                                        >  2:757970/1‑150 (MQ=255)
                                               gCATTTGCCCGCACCTTGTTGCCCGGTGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAAcg                                                                                                        >  2:369488/1‑150 (MQ=255)
                                                    tGCCCGCACCTTGTTGCCCGGTGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCt                                                                                                   <  1:138891/150‑1 (MQ=255)
                                                    tGCCCGCACCTTGTTGCCCGGTGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCt                                                                                                   <  2:616079/150‑1 (MQ=255)
                                                    tGCCCGCACCTTGTTGCCCGGGGAAGTGCTGGTCACCGCGCAACATCTCGACGATCACTGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCt                                                                                                   <  1:332420/150‑1 (MQ=255)
                                                     gCCCGCACCTTGTTGCCCGGTGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGGGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCtt                                                                                                  <  1:557885/150‑1 (MQ=255)
                                                           aCCTTGTTGCCCGGTGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGc                                                                                            <  1:858384/150‑1 (MQ=255)
                                                                 ttgCCCGGTGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTg                                                                                      <  2:1169608/150‑1 (MQ=255)
                                                                  tgCCCGGTGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGc                                                                                     >  2:1361026/1‑150 (MQ=255)
                                                                  tgCCCGGTGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGc                                                                                     >  1:1337470/1‑150 (MQ=255)
                                                                   gCCCGGTGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCt                                                                                    >  2:1451527/1‑150 (MQ=255)
                                                                   gCCCGGTGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTAGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTgat                                                                                    >  2:488608/1‑148 (MQ=255)
                                                                       ggTGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGcc                                                                                <  2:44302/150‑1 (MQ=255)
                                                                        gTGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGccc                                                                               <  1:1092370/150‑1 (MQ=255)
                                                                          gAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCCGc                                                                             >  2:749862/1‑150 (MQ=255)
                                                                            aGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCCGCAc                                                                           >  2:133149/1‑150 (MQ=255)
                                                                                gtGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCCGCACGCgg                                                                       <  2:1347087/149‑1 (MQ=255)
                                                                                  ggTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCCGCACGCgggg                                                                     >  2:1002141/1‑150 (MQ=255)
                                                                                  ggTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCCGCACGCgggg                                                                     <  2:1534515/150‑1 (MQ=255)
                                                                                   gTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGGGGCCCTGCCGGGCGGTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCCGCACGCggggg                                                                    <  2:1139354/150‑1 (MQ=255)
                                                                                    tCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCCGCACGCgggggg                                                                   <  2:468421/150‑1 (MQ=255)
                                                                                      aCCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCCGCACGCGGGGGGaa                                                                 >  2:486135/1‑150 (MQ=255)
                                                                                       ccGCGCAACATCTCGACGCTCACTGTGACACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCCGCACGCGGGGGGAAc                                                                <  1:286578/150‑1 (MQ=255)
                                                                                        cgcgCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCCGCACGCGGGGGGAACt                                                               >  1:1550940/1‑150 (MQ=255)
                                                                                        cgcgCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCCGCACGCGGGGGGAACt                                                               <  1:23209/150‑1 (MQ=255)
                                                                                          cgcAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCCGCACGCGGGGGGAACTgg                                                             >  2:139043/1‑150 (MQ=255)
                                                                                             aaCATCTCGACGATCAATGTGAAACCTAGCTGCTGGCGCTAACACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGACGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCCGCACGCGGGGGGAACTGGTGc                                                          <  1:323682/150‑1 (MQ=255)
                                                                                               cATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCCGCACGCGGGGGGAACTGGTGCAg                                                        <  2:963637/150‑1 (MQ=255)
                                                                                                aTCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCCGCACGCGGGGGGAACTGGTGCAGt                                                       <  1:710169/150‑1 (MQ=255)
                                                                                                 tctcGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCCGCACGCGGGGGGAACTGGTGCAGTg                                                      <  1:1393961/150‑1 (MQ=255)
                                                                                                  cacgacgaTCAATGAGAAACCATTCTGCTGGCGCTAAACCGCGCCAGTGGCCCTGCCGGGCTTTCGGATATGGCGGAAGTCTCGCAGATTCCCCGAACGAGGCTTATTCGCCCGTTGCTCGCCCGCACGCGGTGGGAACTGGTGCAGTgg                                                     >  2:228154/3‑150 (MQ=255)
                                                                                                    cgacgaTCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCCGCACGCGGGGGGAACTGGTGCAGTGGgc                                                   >  1:1620711/1‑150 (MQ=255)
                                                                                                    cgacgaTCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCCGCACGCGGGGGGAACTGGTGCAGTGGgc                                                   >  2:137935/1‑150 (MQ=255)
                                                                                                     gacgaTCAATGTGAAACCTTTCTGCTGGCGCTAAACCGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCTGGAACGCGGCTTATTCGCCCGTTGCTCGCCCGCACGCGGGGGGAACTGGTGCAGTGGgcg                                                  <  1:681219/150‑1 (MQ=255)
                                                                                                      acgaTCAATGTGAACCCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCCGCCCGCGGGGGGAACTGGTGCAGTGGgcgc                                                 >  2:70020/1‑150 (MQ=255)
                                                                                                       cgaTCAATGTGAATCCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCCGCACGCGGGGGGAACTGGTGCAGGGGGcgcg                                                >  2:904795/1‑150 (MQ=255)
                                                                                                       cgaTCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGATATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCCGCACGCGGGGGGAACTGGTGCAGTGGGcgcg                                                <  2:1600260/150‑1 (MQ=255)
                                                                                                            aaTGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCCGCACGCGGGGGGAACTGGTGCAGTGGGCGCGTCAGt                                           >  2:50417/1‑150 (MQ=255)
                                                                                                                tgAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCCGCACGCGGGGGGAACTGGTGCAGTGGGCGCGTCAGTATGa                                       >  1:1648624/1‑150 (MQ=255)
                                                                                                                 gAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCCGCACGCGGGGGGAACTGGTGCAGTGGGCGCGTCAGTATGAt                                      <  2:557110/150‑1 (MQ=255)
                                                                                                                  aaaCCTTTCTGCTGGCGCTAAACCGCGGCAGTGGCCCTGCCGGGCTTTCGTCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCCTCACGCGGGGGGACCTGGAGCAGTGGGCGCGTCAGTATGAtt                                     >  1:282759/1‑150 (MQ=255)
                                                                                                                   aaCCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCCGCACGCGGGGGGAACTGGTGCAGTGGGCGCGTCAGTATGAttt                                    <  1:133149/150‑1 (MQ=255)
                                                                                                                     ccTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCCGCACGCGGGGGGAACTGGTGCAGTGGGCGCGTCAGTATGATTTAc                                  >  1:293034/1‑150 (MQ=255)
                                                                                                                      cTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCGTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCCGCACGCGGGGGGAACTGGTGCAGTGGGCGCGTCAGTATGATTTACg                                 <  2:1626453/150‑1 (MQ=255)
                                                                                                                      cTTTCGGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGGCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCCGCACGCGGGGGGAACTGGTGCAGGGGGCGCGTCAGTATGATTTACg                                 <  1:308896/150‑1 (MQ=255)
                                                                                                                        ttCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCCGCACGCGGGGGGAACTGGTGCAGTGGGCGCGTCAGTATGATTTACGCt                               <  1:429478/150‑1 (MQ=255)
                                                                                                                         tCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGCACGCGGCTTATTCGCCCGTTGCTCGCCCGCACGCGGGGGGAGCTGGTGCAGTGGGCGCGTCAGTATGATTTACGCTg                              >  1:1706916/1‑150 (MQ=255)
                                                                                                                             ctgGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCCGCACGCGGGGGGAACTGGTGCAGTGGGCGCGTCAGTATGATTTACGCTGGAtt                          >  1:1667113/1‑150 (MQ=255)
                                                                                                                               ggCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCCGCACGCGGGGGGAACTGGTGCAGTGGGCGCGTCAGTATGATTTACGCTGGATTGa                        >  2:1632610/1‑150 (MQ=255)
                                                                                                                                 cgcTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCCGCACGCGGGGGGAACTGGTGCAGTGGGCGCGTCAGTATGATTTACGCTGGATTGAAg                      >  1:1349228/1‑150 (MQ=255)
                                                                                                                                  gcTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCCGCACGCGGGGGGAACTGGTGCAGTGGGCGCGTCAGTATGATTTACGCTGGATTGAAGa                     <  2:1545394/150‑1 (MQ=255)
                                                                                                                                   cTAAACCGCGGCAGGGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCGCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCCGCACGCGGGGGGAACTGGTGCAGTGGGCGCGTCAGTATGATTTACGCTGGATTGAAGAc                    <  2:289801/150‑1 (MQ=255)
                                                                                                                                     aaaaCGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCCGCACGCGGGGGGAACTGGTGCAGTGGGCGCGTCAGTATGATTTACGCTGGATTGAAGACGa                  >  1:588347/1‑150 (MQ=255)
                                                                                                                                        gcccgGCAGGGCCACTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCCGCACGCGGGGGGAACTGGTGCAGTGGGCGCGTCAGTATGATTTACGCTGGATTGAAGACGAAAg               >  1:396521/4‑150 (MQ=255)
                                                                                                                                         cccgGCAGGGCCACTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCCGCACGCGGGGGGAACTGGTGCAGTGGGCGCGTCAGTATGATTTACGCTGGATTGAAGACGAAAGt              >  1:1185295/3‑150 (MQ=255)
                                                                                                                                             gCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCCGCACGCGGGGGGAACTGGTGCAGTGGGCGCGTCAGTATGATTTACGCTGGATTGAAGACGAAAGTAATc          >  1:863283/1‑150 (MQ=255)
                                                                                                                                              cAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCCGCACGCGGGGGGAACTGGTGCAGTGGGCGCGTCAGTATGATTTACGCTGGATTGAAGACGAAAGTAATCa         >  1:659663/1‑150 (MQ=255)
                                                                                                                                                  ggCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCCGCACGCGGGGGGAACTGGTGCAGTGGGCGCGTCAGTATGATTTACGCTGGATTGAAGACGAAAGTAATCAGgac     >  2:1271574/1‑150 (MQ=255)
                                                                                                                                                  ggCCCTGCCGGGCTTTCGGCTATGGCGGAAGGCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCCGCACGCGGGGGGAACTGGTGCAGTGGGCGCGTCAGTATGATTTACGCTGGATTGAAGACGAAAGTAATCAGgac     <  2:254932/150‑1 (MQ=255)
                                                                                                                                                   gCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCCGCACGCGGGGGGAACTGGTGCAGTGGGCGCGTCAGTATGATTTACGCTGGATTGAAGACGAAAGTAATCAGgacg    <  1:50417/150‑1 (MQ=255)
                                                                                                                                                   gCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGATTATTCGCCCGTTGCTCGCCCGCACGCGGGGGGAACTGGTGCAGTGGGCGCGTCAGTATGATTTACGCTGGATTGAAGACGAAAGTAATCAGgacg    <  1:1082131/150‑1 (MQ=255)
                                                                                                                                                     ccTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCCGCACGCGGGGGGAACTGGTGCAGTGGGCGCGTCAGTATGATTTACGCTGGATTGAAGACGAAAGTAATCAGgacgac  <  2:1648624/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AAGAAGGACTGGGCATTGAGGCCCAGGCGCGGCAGGCACGTTATCAGGCATTTGCCCGCACCTTGTTGCCCGGTGAAGTGCTGGTCACCGCGCAACATCTCGACGATCAATGTGAAACCTTTCTGCTGGCGCTAAAACGCGGCAGTGGCCCTGCCGGGCTTTCGGCTATGGCGGAAGTCTCGGAGTTTGCCGGAACGCGGCTTATTCGCCCGTTGCTCGCCCGCACGCGGGGGGAACTGGTGCAGTGGGCGCGTCAGTATGATTTACGCTGGATTGAAGACGAAAGTAATCAGGACGAC  >  NC_000913/212581‑212879

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑