Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 219,011 G→A 5.0% P195L (CCG→CTG)  tsaA ← tRNA‑Thr(GGU) m(6)t(6)A37 methyltransferase, SAM‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913219,0110GA 5.0% 389.3 / 4.0 118P195L (CCG→CTG) tsaAtRNA‑Thr(GGU) m(6)t(6)A37 methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base G (50/62);  new base A (3/3);  total (53/65)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.61e-01

Only 100 of 124 total aligned reads displayed.

ATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCTTCTCCAGCGTCAAAAGCTGCTTTTCGACCTCTGCGGTAAAACTCACCGCCATCTCTGCAGCTGGCGCACTTTGCGCATAGCTGGCACTGGCATCGGGA  >  NC_000913/218863‑219160
                                                                                                                                                    |                                                                                                                                                     
aTGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCgg                                                                                                                                                      >  1:132104/1‑150 (MQ=255)
 tGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTAAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCggg                                                                                                                                                     >  2:79850/1‑150 (MQ=255)
  gTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGt                                                                                                                                                    >  1:744415/1‑150 (MQ=255)
    cAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCCGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGGTAAAATCAAGTAGCCAGACGGCAGCGGTTTTGCCCGTGTCCTCACCTGGACGATAGGCCGGGCGCGGGTcc                                                                                                                                                  >  1:1130569/1‑150 (MQ=255)
    cAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCAGTTTCCTCACCTTAACGATAGGCCGGGCGCGGGTcc                                                                                                                                                  >  1:1635372/1‑150 (MQ=255)
     aaaaGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCt                                                                                                                                                 >  1:683304/1‑150 (MQ=255)
       aaGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGCCGGCATAGGTATTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTgc                                                                                                                                               >  1:1091954/1‑150 (MQ=255)
        agagAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTgcg                                                                                                                                              <  2:1408682/150‑1 (MQ=255)
             cgggCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCAGGACCCGCGcccgg                                                                                                                                         <  2:389544/149‑4 (MQ=255)
             aGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCAGGACCCGCGcccgg                                                                                                                                         <  1:1592916/150‑4 (MQ=255)
             aGGGCGGGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGGGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTCCGATAGGCCGGGCGCAGGACCCGCGcccgg                                                                                                                                         <  1:1599275/150‑4 (MQ=255)
                gCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCTGGCGCGGGTCCTGCGCCAGTACt                                                                                                                                      >  2:252509/1‑150 (MQ=255)
                gCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACt                                                                                                                                      <  2:1205798/150‑1 (MQ=255)
                 cGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGCCGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTGCTGCGCCAGGAcgt                                                                                                                                     >  2:827893/1‑148 (MQ=255)
                 cGTGAATTTAACGCGGTTCCAGCGCAAAGACATCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGACAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACtt                                                                                                                                     >  1:821309/1‑150 (MQ=255)
                     aaTTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTcgcg                                                                                                                                 >  1:272055/1‑150 (MQ=255)
                     aaTTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTcgcg                                                                                                                                 <  1:109470/150‑1 (MQ=255)
                     aaTTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTcgcg                                                                                                                                 <  2:372418/150‑1 (MQ=255)
                      aTTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCgg                                                                                                                                <  2:337797/150‑1 (MQ=255)
                       tttAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGa                                                                                                                               <  2:119615/150‑1 (MQ=255)
                       tttAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATACGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGa                                                                                                                               <  1:252080/150‑1 (MQ=255)
                        ttAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGGCGGGGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGGCCTGCGCCAGTACTTCGCGGAt                                                                                                                              <  1:854286/150‑1 (MQ=255)
                        ttAACGCGGTTCCAGCGCAAAGACTTCAAAACATGCGTCGGTGACGCGCCAGCGAACTTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGCGTCCTGCGCCAGTACTTCGCGGAt                                                                                                                              >  1:45791/1‑150 (MQ=255)
                          aaCGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATaa                                                                                                                            <  2:704734/150‑1 (MQ=255)
                             gcgGTTCCAGCGCAAAGACTTCAACACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCAAAGGTATTGCCCGTTTCCTCACCATTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACa                                                                                                                         >  1:1163539/1‑150 (MQ=255)
                                gTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCg                                                                                                                      <  2:170519/150‑1 (MQ=255)
                                gTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCg                                                                                                                      <  2:633028/150‑1 (MQ=255)
                                   ccAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCTAACCTTAAAATCATGTAGCCAGACGGCATTGGATTTGCCCGTTTCCTCACCTTTACGATAGTCCGGGCGCGGGTCCTGCGACAGTACTTCGCGGATAAACAGCGTTa                                                                                                                   >  1:106198/1‑150 (MQ=255)
                                    cAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCCCCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCTGTACTTCGCGGATAAACAGCGTTaa                                                                                                                  >  2:238584/1‑150 (MQ=255)
                                    cAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTaa                                                                                                                  >  2:741047/1‑150 (MQ=255)
                                     aGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCTGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTGCGCGGATAAACAGCGTTAAc                                                                                                                 <  2:1492781/150‑1 (MQ=255)
                                               cTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAAc                                                                                                       <  1:1286150/150‑1 (MQ=255)
                                                ttCAAAACCTGCGTCGGTGACGCGAAAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGTGTCCTGCGACAGTACTTCGCTGATAAACAGCGTTAACTGCGGATAACg                                                                                                      >  1:1355620/1‑150 (MQ=255)
                                                 tCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGc                                                                                                     >  2:649762/1‑150 (MQ=255)
                                                 tCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGc                                                                                                     >  2:1414353/1‑150 (MQ=255)
                                                 tCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGc                                                                                                     <  1:666067/150‑1 (MQ=255)
                                                  cAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCt                                                                                                    >  1:578223/1‑150 (MQ=255)
                                                  cAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCt                                                                                                    >  2:730709/1‑150 (MQ=255)
                                                     aaCCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTATCCGCAGTTAACGCtgtt                                                                                                 <  1:754040/150‑4 (MQ=255)
                                                         tGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTTCGGATAACGCTTCTCCAg                                                                                             <  1:741047/150‑1 (MQ=255)
                                                          gCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCTTCTCCAGc                                                                                            <  2:617576/150‑1 (MQ=255)
                                                             tCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCTTCTCCAGCGTc                                                                                         >  1:585822/1‑150 (MQ=255)
                                                               ggTGACGCGCCAGCGAACGTTAAACTCATGTAGCCACACGCCATAGGATTAACACGATACCTCACCATTACGATAGGCCGGGCGCAGGTCCTGCGCCAGTACTTCGCGCATAAACAGCGTTAACTGCGCATACCGCATCTCCAGCGTCaa                                                                                       >  2:751537/1‑150 (MQ=255)
                                                                  gACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCTTCTCCAGCGTCAAAAg                                                                                    <  2:1248441/150‑1 (MQ=255)
                                                                   aCGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCTTCTCCAGCGTCAAAAgc                                                                                   <  1:694011/150‑1 (MQ=255)
                                                                    cgcgCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCTTCTCCAGCGTCAAAAgct                                                                                  >  1:83680/1‑150 (MQ=255)
                                                                       gcCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCTTCTCCAGCGTCAAAAgctgct                                                                               <  1:1232951/150‑1 (MQ=255)
                                                                        ccAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCTTCTCCAGCGTCAAAAGCTGCtt                                                                              >  2:413527/1‑150 (MQ=255)
                                                                        ccAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCTTCTCCAGCGTCAAAAGCTGCtt                                                                              >  1:1248119/1‑150 (MQ=255)
                                                                             gAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCTTCTCCAGCGTCAAAAGCTGCTTTTCGa                                                                         >  1:309011/1‑150 (MQ=255)
                                                                              aaCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCTTCTCCAGCGTCAAAAGCTGCTTTTCGAc                                                                        <  2:1256676/150‑1 (MQ=255)
                                                                               aCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCTTCTCCAGCGTCAAAAGCTGCTTTTCGAcc                                                                       <  2:1520040/150‑1 (MQ=255)
                                                                               aCGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCTTCTCCAGCGTCAAAAGCTGCTTTTCGAcc                                                                       <  1:1092219/150‑1 (MQ=255)
                                                                                cGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCTTCTCCAGCGTCAAAAGCTGCTTTTCGACct                                                                      >  1:1552205/1‑150 (MQ=255)
                                                                                 gTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCTTCTCCAGCGTCAAAAGCTGCTTTTCGACctc                                                                     >  2:1633351/1‑150 (MQ=255)
                                                                                 gTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCTTCTCCAGCGTCAAAAGCTGCTTTTCGACctc                                                                     >  1:1013375/1‑150 (MQ=255)
                                                                                  ttAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCTTCTCCAGCGTCAAAAGCTGCTTTTCGACctct                                                                    >  2:936340/1‑150 (MQ=255)
                                                                                    aaaaTCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCTTCTCCAGCGTCAAAAGCTGCTTTTCGACCTCTGc                                                                  <  2:578223/150‑1 (MQ=255)
                                                                                    aaaaTCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCTTCTCCAGCGTCAAAAGCTGCTTTTCGACCTCTGc                                                                  <  2:1163539/150‑1 (MQ=255)
                                                                                    aaaaTCATGTAGCCAGACGGCATAGGTTTTGCCCGTTCCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATTAACCGCGTTAACTGCGGATAACGCTTCTCCAGCGTCAAAAGCTGCTTTTCGCCCTCTGc                                                                  >  1:855581/1‑150 (MQ=255)
                                                                                       aTCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCTTCTCCAGCGTCAAAAGCTGCTTTTCGACCTCTGCGGt                                                               <  2:683304/150‑1 (MQ=255)
                                                                                        tCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCTTCTCCAGCGTCAAAAGCTGCTTTTCGACCTCTGCGGTa                                                              >  2:134022/1‑150 (MQ=255)
                                                                                         cATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCTTCTCCAGCGTCAAAAGCTGCTTTTCGACCTCTGCGGTaa                                                             <  1:287299/150‑1 (MQ=255)
                                                                                         cATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCTTCTCCAGCGTCAAAAGCTGCTTTTCGACCTCTGCGGTaa                                                             >  2:497939/1‑150 (MQ=255)
                                                                                          aTGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCTTCTCCAGCGTCAAAAGCTGCTTTTCGACCTCTGCGGTaaa                                                            <  2:1265477/150‑1 (MQ=255)
                                                                                            ggaGCCAGACGGCATCGGGTTTGCCCTTTTCCTCACCTTTACTATAGGCCGGGCGCGGGTACTGCGCCAGTACTTCGCGGATACTCAGAGTTAACTGCGGATAACGATTCTCCAGCGTCAAATGCTGCTTTTCGACCTCTGCGGTAATAc                                                          <  2:1130569/148‑1 (MQ=255)
                                                                                            gTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCTTCTCCAGCGTCAAAAGCTGCTTTTCGACCTCTGCGGTAAAAc                                                          <  2:1175960/150‑1 (MQ=255)
                                                                                               cccAGACGGCATAGGTTTTGCGCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGTATAACGCTTCTCCAGCGTCAAAAGCTGCTTTTCGACCTCTGCGGTAAAACTCa                                                       <  2:45791/149‑1 (MQ=255)
                                                                                                 cAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCTTCTCCAGCGTCAAAAGCTGCTTTTCGACCTCTGCGGTAAACCTcccc                                                     >  2:921631/1‑150 (MQ=255)
                                                                                                 cAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCTTCTCCAGCGTCAAAAGCTGCTTTTCGACCTCTGCGGTAAAACTCAcc                                                     >  2:1287235/1‑150 (MQ=255)
                                                                                                   gACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCTTCTCCAGCGTCAAAAGCTGCTTTTCGACCTCTGCGGTAAAACTCACCGc                                                   <  2:557938/150‑1 (MQ=255)
                                                                                                         aTAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCTTCTCCAGCGTCAAAAGCTGCTTTTCGACCTCTGCGGTAAAACTCACCGCCAtctc                                             <  1:1005516/150‑1 (MQ=255)
                                                                                                         aTAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCTTCTCCAGCGTCAAAAGCTGCTTTTCGACCTCTGCGGTAAAACTCACCGCCAtctc                                             <  2:821309/150‑1 (MQ=255)
                                                                                                         aTAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCTTCTCCAGCGTCAAAAGCTGCTTTTCGACCCCTGCGGTAAAACTCACCGCCAtctc                                             <  1:420738/150‑1 (MQ=255)
                                                                                                           aGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCTTCTCCAGCGTCAAAAGCTGCTTTTCGACCTCTGCGGTAAAACTCACCGCCATCTCTg                                           <  2:544535/150‑1 (MQ=255)
                                                                                                            ggTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCTTCTCCAGCGTCAAAAGCTGCTTTTCGACCTCTGCGGTAAAACTCACCGCCATCTCTGc                                          >  2:874515/1‑150 (MQ=255)
                                                                                                            ggTTTTGCCAGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCTTCTCCAGCGTCAAAAGCTGCTTTTCGACCTCTGCGGTAAAACTCACCGCCATCTCTGc                                          >  2:1391452/1‑150 (MQ=255)
                                                                                                             gTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCTTCTCCAGCGTCAAAAGCTGCTTTTCGACCTCTGCGGTAAAACTCACCGCCATCTCTGCa                                         >  1:1222791/1‑150 (MQ=255)
                                                                                                             gTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCTTCTCCAGCGTCAAAAGCTGCTTTTCGACCTCTGCGGTAAAACTCACCGCCATCTCTGCa                                         <  2:196128/150‑1 (MQ=255)
                                                                                                              ttttGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCTTCTCCAGCGTCAAAAGCTGCTTTTCGACCTCTGCGGTAAAACTCACCGCCATCTCTGCAg                                        >  1:275058/1‑150 (MQ=255)
                                                                                                              ttttGCCCGTTTCCTCACCTTTAAGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCTTCTCCAGCGTCAAAAGCTGCTTTTCGACCTCTGCGGTAAAACTCACCGCCATCTCTGCAg                                        <  2:838385/150‑1 (MQ=255)
                                                                                                                 tGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCTTCTCCAGCGTCAAAAGCTGCTTTTCGACCTCTGCGGTAAAACTCACCGCCATCTCTGCAGCTg                                     >  1:1422724/1‑150 (MQ=255)
                                                                                                                 tGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCTTCTCCAGCGTCAAAAGCTGCTTTTCGACCTCTGCGGTAAAACTCACCGCCATCTCTGCAGCTg                                     >  1:1099988/1‑150 (MQ=255)
                                                                                                                   cccGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCTTCTCCAGCGTCAAAAGCTGCTTTTCGACCTCTGCGGTAAAACTCACCGCCATCTCTGCAGCTGgc                                   <  2:204163/150‑1 (MQ=255)
                                                                                                                         tCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCTTCTCCAGCGTCAAAAGCTGCTTTTCGACCTCTGCGGTAAAACTCACCGCCATCTCTGCAGCTGGCGCACtt                             >  1:958742/1‑150 (MQ=255)
                                                                                                                             cACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCTTCTCCAGCGTCAAAAGCTGCTTTTCGACCTCTGCGGTAAAACTCACCGCCATCTCTGCAGCTGGCGCACTTTgcg                         <  2:1248119/150‑1 (MQ=255)
                                                                                                                              aCCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCTTCTCCAGCGTCAAAAGCTGCTTTTCGACCTCTGCGGTAAAACTCACCGCCATCTCTGCAGCTGGCGCACTTTgcgc                        <  1:1414353/150‑1 (MQ=255)
                                                                                                                               ccTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCTTCTCCAGCGTCAAAAGCTGCTTTTCGACCTCTGCGGTAAAACTCACCGCCATCTCTGCAGCTGGCGCACTTTGCGCa                       >  2:42212/1‑150 (MQ=255)
                                                                                                                                 tttACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCTTCTCCAGCGTCAAAAGCTGCTTTTCGACCTCTGCGGTAAAACTCACCGCCATCTCTGCAGCTGGCGCACTTTGCGCATa                     <  2:309011/150‑1 (MQ=255)
                                                                                                                                      gATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCTTCTCCAGCGTCAAAAGCTGCTTTTCGACCTCTGCGGTAAAACTCACCGCCATCTCTGCAGCTGGCGCACTTTGCGCATAGctgg                >  1:52258/1‑150 (MQ=255)
                                                                                                                                       aTAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCTTCTCCAGCGTCAAAAGCTGCTTTTCGACCTCTGCGGTAAAACTCACCGCCATCTCTGCAGCTGGCGCACTTTGCGCATAGctggc               <  2:132104/150‑1 (MQ=255)
                                                                                                                                       aTAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCTTCTCCAGCGTCAAAAGCTGCTTTTCGACCTCTGCGGTAAAACTCACCGCCATCTCTGCAGCTGGCGCACTTTGCGCATAGctggc               <  2:1323368/150‑1 (MQ=255)
                                                                                                                                          ggCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCTTCTCCAGCGTCAAAAGCTGCTTTTCGACCTCTGCGGTAAAACTCACCGCCATCTCTGCAGCTGGCGCACTTTGCGCATAGctggcact            <  1:53334/150‑1 (MQ=255)
                                                                                                                                          ggCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCTTCTCCAGCGTCAAAAGCTGCTTTTCGACCTCTGCGGTAAAACTCACCGCCATCTCTGCAGCTGGCGCACTTTGCGCATAGctggcact            >  2:338595/1‑150 (MQ=255)
                                                                                                                                            ccGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCTTCTCCAGCGTCAAAAGCTGCTTTTCGACCTCTGCGGTAAAACTCACCGCCATCTCTGCAGCTGGCGCACTTTGCGCATAGctggcactgg          <  2:733776/150‑1 (MQ=255)
                                                                                                                                            ccGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCTTCTCCAGCGTCAAAAGCTGCTTTTCGACCTCTGCGGTAAAACTCACCGCCATCTCTGCAGCTGGCGCACTTTGCGCATAGctggcactgg          <  1:721512/150‑1 (MQ=255)
                                                                                                                                             ctggCGCAGGACCCGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCTTCTCCAGCGTCAAAAGCTGCTTTTCGACCTCTGCGGTAAAACTCACCGCCATCTCTGCAGCTGGCGCACTTTGCGCATAGctggcactggc         >  1:383105/3‑150 (MQ=255)
                                                                                                                                                   cgGGTCCTGCGCCAGTACTTTGCGGATAAACAGCGTTAACTGCGGATAACGCTTCTCCAGCGTCAAAAGCTGCTTTTCGACCTCTGCGGTAAAACTCACCGCCATCTCTGCAGCTGGCGCACTTTGCGCATAGCTGGCACTGGCATCggg   >  2:663165/1‑150 (MQ=255)
                                                                                                                                                   cgGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCTTCTCCAGCGTCAAAAGCTGCTTTTCGACCTCTGCGGTAAAACTCACCGCCATCTCTGCAGCTGGCGCACTTTGCGCATAGCTGGCACTGGCATCggg   >  1:491558/1‑150 (MQ=255)
                                                                                                                                                    gggTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCTTCTCCAGCGTCAAAAGCTGCTTTTCGACCTCTGCGGTAAAACTCACCGCCATCTCTGCAGCTGGCGCACTTTGCGCATAGCTGGCACTGGCATCGGGa  <  1:827893/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
ATGTCAAAAGAGAAGGGCGTGAATTTAACGCGGTTCCAGCGCAAAGACTTCAAAACCTGCGTCGGTGACGCGCCAGCGAACGTTAAAATCATGTAGCCAGACGGCATAGGTTTTGCCCGTTTCCTCACCTTTACGATAGGCCGGGCGCGGGTCCTGCGCCAGTACTTCGCGGATAAACAGCGTTAACTGCGGATAACGCTTCTCCAGCGTCAAAAGCTGCTTTTCGACCTCTGCGGTAAAACTCACCGCCATCTCTGCAGCTGGCGCACTTTGCGCATAGCTGGCACTGGCATCGGGA  >  NC_000913/218863‑219160

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: