Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,456,861 A→T 5.4% intergenic (‑49/+154) sixA ← / ← fadJ phosphohistidine phosphatase/enoyl‑CoA hydratase/epimerase and isomerase/3‑hydroxyacyl‑CoA dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,456,8610AT 5.4% 239.2 / 2.7 84intergenic (‑49/+154)sixA/fadJphosphohistidine phosphatase/enoyl‑CoA hydratase/epimerase and isomerase/3‑hydroxyacyl‑CoA dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (35/44);  new base T (3/2);  total (38/46)
Fisher's exact test for biased strand distribution p-value = 6.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.04e-01

TCAGGCGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCTTTGAC  >  NC_000913/2456713‑2457010
                                                                                                                                                    |                                                                                                                                                      
tCAGGCGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAAc                                                                                                                                                       <  1:1496211/150‑1 (MQ=255)
 cAGGCGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAAcc                                                                                                                                                      >  1:1118858/1‑150 (MQ=255)
 cAGGCGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAAcc                                                                                                                                                      >  1:290875/1‑150 (MQ=255)
   ggCGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCag                                                                                                                                                    >  1:737641/1‑150 (MQ=255)
   ggCGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCag                                                                                                                                                    <  1:1131082/150‑1 (MQ=255)
   ggCGAGATTCGTCACAACCAATAGTGGTCAGAGGACGAACGGAATCAGTGCCGGCATCGAGGGCTGCGTCGCCGTGACGCTAGATAAATACATGCATATTGCACCGCTATTGTTAACCAGATTCGCCAGTTTTGTTTCATATGAAAcaac                                                                                                                                                    >  1:1439982/1‑147 (MQ=255)
     cGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCaga‑g                                                                                                                                                 >  1:273923/1‑150 (MQ=255)
       agaTTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGa‑gaa                                                                                                                                               >  2:271187/1‑150 (MQ=255)
       agaTTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGa‑gaa                                                                                                                                               >  2:1700686/1‑150 (MQ=255)
        gaTTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGa‑gaaa                                                                                                                                              >  2:703562/1‑150 (MQ=255)
             gTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGA‑GAAAGaaaa                                                                                                                                         <  2:1473623/150‑1 (MQ=255)
              tCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTTCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAAc                                                                                                                                        <  1:503268/150‑1 (MQ=255)
              tCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAAc                                                                                                                                        <  2:1690360/150‑1 (MQ=255)
              tCACAACCATAAGTGGTCAGCGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATTTGAAACCAGA‑GAAAGAAAAc                                                                                                                                        <  2:1558412/150‑1 (MQ=255)
                 caACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTC‑TCTGGTTTCAGATGAAAGAAAACggg                                                                                                                                     <  1:1714808/150‑2 (MQ=38)
                 caACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATAAGCCCGTTTTATTTCATCTGAAACCAGA‑GAAAGAAAACgct                                                                                                                                     >  2:131389/1‑148 (MQ=255)
                     cATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGACGCCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTAAACCAGATTCGCCCGTTATCTTTCATCTGAAACCAGA‑GAATGAAAAGGGTGGcc                                                                                                                                 <  2:1439982/150‑1 (MQ=255)
                      aTTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAACGGTGGCCg                                                                                                                                <  2:1669516/150‑1 (MQ=255)
                         aGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAAAGGTGGCCGGCa                                                                                                                             >  2:1585725/1‑150 (MQ=255)
                            ggTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCAttt                                                                                                                          >  2:1449131/1‑149 (MQ=255)
                                 gagGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCt                                                                                                                     <  1:435791/150‑1 (MQ=255)
                                   ggACGAACGGAATCACTGGCGGCATCGCGGGCTGCGTCGCCGTGACGCATGATAAACACTTGCATATTGCACCGCTGTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAGCGGTGGCCGGCAGTGTGCCTTa                                                                                                                   >  1:982931/1‑150 (MQ=255)
                                      cGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTTACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTATcc                                                                                                                <  1:1291992/150‑1 (MQ=255)
                                      cGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTGCATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTATcc                                                                                                                >  2:1026022/1‑150 (MQ=255)
                                        aaCGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTATTTAATCTGTAAACCGA‑GAAAGAAAAAGGTGGGCGGCCTTGTGGCTTATACaa                                                                                                              >  1:1524624/1‑149 (MQ=255)
                                           ggAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCa                                                                                                           >  1:400436/1‑150 (MQ=255)
                                              aTCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCg                                                                                                        <  2:1106357/150‑1 (MQ=255)
                                               tCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGa                                                                                                       <  1:1657380/150‑1 (MQ=255)
                                                cACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGaa                                                                                                      >  2:1380892/1‑150 (MQ=255)
                                                 aCTTGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGTTTCGCCCGTTTTCTTTCATCTGAATCCTGA‑GAAAGAATACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAAt                                                                                                     <  1:4796/150‑1 (MQ=255)
                                                  cTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGTTTATAACTTGCATATTGCACCGCTGTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATg                                                                                                    <  1:131389/150‑1 (MQ=255)
                                                   tGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGa                                                                                                   <  2:1657684/150‑1 (MQ=255)
                                                    ggcggcATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGaa                                                                                                  >  2:645648/1‑150 (MQ=255)
                                                     gcggcATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTATTTCATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGaaa                                                                                                 >  1:1340078/1‑150 (MQ=255)
                                                      cggcATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAAc                                                                                                <  2:290875/150‑1 (MQ=255)
                                                        gcATCGAGGGCTGCGTCGCAGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGc                                                                                              <  2:12604/150‑1 (MQ=255)
                                                          aTCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTg                                                                                            >  1:236731/1‑150 (MQ=255)
                                                           tCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGt                                                                                           <  2:737641/150‑1 (MQ=255)
                                                            cGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGtt                                                                                          <  2:240588/150‑1 (MQ=255)
                                                            cGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGtt                                                                                          <  2:273923/150‑1 (MQ=255)
                                                             gAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCGCCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAACCGCTGttt                                                                                         >  1:217017/1‑150 (MQ=255)
                                                                tcatGCGTCACGGCGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGtttttt                                                                                      >  1:1230435/4‑150 (MQ=255)
                                                                  cTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTAc                                                                                    <  2:1049838/150‑1 (MQ=255)
                                                                  cTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTAc                                                                                    <  1:497403/150‑1 (MQ=255)
                                                                    gCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCt                                                                                  >  2:916076/1‑150 (MQ=255)
                                                                     cGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTc                                                                                 <  1:1357771/150‑1 (MQ=255)
                                                                          ccGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATgg                                                                            <  2:1234964/150‑1 (MQ=255)
                                                                           cGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGc                                                                           <  1:1628741/150‑1 (MQ=255)
                                                                            gTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCg                                                                          <  2:1253535/150‑1 (MQ=255)
                                                                                cGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAg                                                                      <  1:703562/150‑1 (MQ=255)
                                                                                    tGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGtata                                                                  <  1:557311/150‑1 (MQ=255)
                                                                                     taTAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAg                                                                 <  2:1571285/149‑1 (MQ=255)
                                                                                           aaCTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAata                                                           <  2:1411554/150‑1 (MQ=255)
                                                                                             cTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATg                                                         <  1:978301/150‑1 (MQ=255)
                                                                                               tGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCg                                                       <  2:955466/150‑1 (MQ=255)
                                                                                                  atatTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGttt                                                    <  2:1412359/150‑1 (MQ=255)
                                                                                                   tatTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTa                                                   >  1:957909/1‑150 (MQ=255)
                                                                                                    atTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTAc                                                  <  2:1349912/150‑1 (MQ=255)
                                                                                                    atTGCACCGCTTTTGTTAACAAGATTCGCCCGTTTTCTTTCATCTGAAACCACA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTAc                                                  <  2:1082791/150‑1 (MQ=255)
                                                                                                      tGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACAt                                                >  1:383806/1‑150 (MQ=255)
                                                                                                        cACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACAttt                                              <  1:271187/150‑1 (MQ=255)
                                                                                                        cACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACAttt                                              <  1:1303430/150‑1 (MQ=255)
                                                                                                        cACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACAttt                                              <  1:1700686/150‑1 (MQ=255)
                                                                                                         aCCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACAtttt                                             >  1:1231073/1‑150 (MQ=255)
                                                                                                           cGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATAAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGa                                           >  1:438459/1‑150 (MQ=255)
                                                                                                            gCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGAc                                          <  1:1069606/150‑1 (MQ=255)
                                                                                                               ttttttAACAAGATTATCCCGTTTTCTTTCATCTGAAACAAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATAAGCGTTTACATTTTGACCaa                                       <  2:838659/150‑1 (MQ=255)
                                                                                                                 tGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAaca                                     >  2:491551/1‑150 (MQ=255)
                                                                                                                 tGTTAACCAGATTCGCCCGTTTTAATTCATCTGAAACCAGA‑GAAAGAAAAAGGTGGCCGGCAATGTGCCTTATCCCTTCAACCAAATAAACCCTGTTTTTTACCACAAAGTCGTAATTATATTCAAAATTCGTTAAAATTTTGACCAacc                                     >  2:1563111/1‑149 (MQ=255)
                                                                                                                       ccAGATTCGCCCGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATACACCGAATGAAACGCTGTTATTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTCCATTTTGACCAACACTCAGc                               >  2:958792/1‑150 (MQ=255)
                                                                                                                            ttCGCCCGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCTTTGTGCCTTATCCATTCACCGAATGAACCGGTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGACATTc                          >  1:843066/1‑150 (MQ=255)
                                                                                                                              ccaccGTTTTCTTTC‑TCTGGTTTCAGATGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAg                        >  1:1346264/4‑150 (MQ=38)
                                                                                                                               gCCCGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTATACATTTTGACCAACACTCCGCCATTCAgc                       >  1:473100/1‑150 (MQ=255)
                                                                                                                                accGTTTTCTTTC‑TCTGGTTTCAGATGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAgcg                      >  1:493945/2‑150 (MQ=38)
                                                                                                                                accGTTTTCTTTC‑TCTGGTTTCAGATGAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAgcg                      >  1:996166/2‑150 (MQ=38)
                                                                                                                                 ccGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAgcgc                     <  1:870345/150‑1 (MQ=255)
                                                                                                                                       tctttcATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTc               >  1:315131/1‑150 (MQ=255)
                                                                                                                                        ctttcATCTGAAACCAGA‑GAAAGTAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCAGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCa              <  2:1518018/150‑1 (MQ=255)
                                                                                                                                        ctttcATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCa              <  2:1524624/150‑1 (MQ=255)
                                                                                                                                        ctttcATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCa              <  2:1017040/150‑1 (MQ=255)
                                                                                                                                         tttcATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCat             >  2:765811/1‑150 (MQ=255)
                                                                                                                                           tcATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCatat           >  1:468492/1‑150 (MQ=255)
                                                                                                                                            cATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGGTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCAtata          >  1:1538824/1‑150 (MQ=255)
                                                                                                                                             aTCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAg         <  1:645648/150‑1 (MQ=255)
                                                                                                                                                  aaaCCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCTTTg    <  2:1340078/150‑1 (MQ=255)
                                                                                                                                                    aCCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCTTTGAc  <  1:1292916/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                      
TCAGGCGAGATTCGTCACAACCATTAGTGGTCAGAGGACGAACGGAATCACTGGCGGCATCGAGGGCTGCGTCGCCGTGACGCATGATAAAAACTTGCATATTGCACCGCTTTTGTTAACCAGATTCGCCCGTTTTCTTTCATCTGAAACCAGA‑GAAAGAAAACGGTGGCCGGCATTGTGCCTTATCCATTCACCGAATGAAACGCTGTTTTTTACCTCAATGGCGTAAGTATAGTCAATATGCGTTTACATTTTGACCAACACTCCGCCATTCAGCGCGGATTCATATAGCTTTGAC  >  NC_000913/2456713‑2457010

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑