Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,457,138 C→G 5.4% V674V (GTG→GTC fadJ ← enoyl‑CoA hydratase/epimerase and isomerase/3‑hydroxyacyl‑CoA dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,457,1380CG 5.4% 241.5 / 4.2 74V674V (GTG→GTCfadJenoyl‑CoA hydratase/epimerase and isomerase/3‑hydroxyacyl‑CoA dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (40/30);  new base G (2/2);  total (42/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.30e-01

CGCGGATTCATATAGCTTTGACCTTCTTATTGCAGGTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCA  >  NC_000913/2456989‑2457283
                                                                                                                                                     |                                                                                                                                                 
cgcgGATTCATATAGCTTTGACCTTCTTATTGCAGGTCAGTTGCAGTTGTTTTCCAAAACCTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAAcc                                                                                                                                                   <  2:473100/150‑1 (MQ=255)
cgcgGATTCATATAGCTTTGACCTTCTTATTGCAGGTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAAcc                                                                                                                                                   <  2:60099/150‑1 (MQ=255)
cgcgGATTCATATAGCTTTGACCTTCTTATTGCAGGTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAAcc                                                                                                                                                   <  1:878849/150‑1 (MQ=255)
cgaggtttCATATAGCTTTGACCTTCTTATTGCAGGTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCTAGGGGTAAAACGGGAACCATACTGGGTGGCAAGTCGTTGCATTATTGCAAcc                                                                                                                                                   <  1:494761/144‑1 (MQ=255)
   ggATTCATATAGCTTTGACCTTCTTATTGCAGGTCAGTTGCAGTTGTTTTCCAAAAACCTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACt                                                                                                                                                <  1:744116/150‑1 (MQ=255)
    gATTCATATAGCTTTGACCTTCTTATTGCAGGTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCAATAATGCAACGACtt                                                                                                                                               <  2:1684919/150‑1 (MQ=255)
       tCATATAGCTTTGACCTTCTTATTGCAGGTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGc                                                                                                                                            <  2:493945/150‑1 (MQ=255)
        cATATAGCTTTGACCTTCTTATTGCAGGTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGcc                                                                                                                                           >  1:1285376/1‑150 (MQ=255)
         atatAGCTTTGACCTTCTTATTGCAGGTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTGGTTGCAATAATGCAACGACttgccac                                                                                                                                          <  2:1598578/150‑6 (MQ=255)
            taGCTTTGACCTTCTTATTGCAGGTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCcgcc                                                                                                                                       >  1:122575/1‑149 (MQ=255)
              gCTTTGACCTTCTTATTGCAGGTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAATCGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCATCCACTTCGCCCGCGcc                                                                                                                                     >  1:125427/1‑150 (MQ=255)
              gCTTTGACCTTCTTATTGCAGGTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGAGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGcc                                                                                                                                     >  1:472616/1‑150 (MQ=255)
                 ttGACCTTCTTATTGCAGGTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCgag                                                                                                                                  <  2:251002/150‑1 (MQ=255)
                 ttGACCTTCTTATTGCAGGTAAGTTGCAGTTGTTTTCGAAAAACTTTCCCCACGCGCGCCCTTCTCGAACAAACGCTCGCATGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCgag                                                                                                                                  <  2:335616/150‑1 (MQ=255)
                   gACCTTCTTATTGCAGGTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCgagag                                                                                                                                >  1:1136595/1‑150 (MQ=255)
                   gACCTTCTTATTGCAGGTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCgagag                                                                                                                                >  1:541086/1‑150 (MQ=255)
                         cttATTGCAGGTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGa                                                                                                                          >  2:1346391/1‑150 (MQ=255)
                         cttATTGCAGGTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGa                                                                                                                          <  1:1085875/150‑1 (MQ=255)
                          ttATTGCAGGTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGat                                                                                                                         <  1:1277437/150‑1 (MQ=255)
                           tATTGCAGGTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGCCCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGTCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGata                                                                                                                        >  2:311821/1‑150 (MQ=255)
                             ttGCAGGTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGAtata                                                                                                                      >  2:211786/1‑150 (MQ=255)
                             ttGCAGGTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGAtata                                                                                                                      <  1:679080/150‑1 (MQ=255)
                               gCAGGTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGc                                                                                                                    <  1:743177/150‑1 (MQ=255)
                                cAGGTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCg                                                                                                                   <  2:377638/150‑1 (MQ=255)
                                  ggTCAGATTCAGTTGTATTCCAAAAACTTACCCCAATCGCGACATTCTCGACAAAACGCTCGCAAGGGGTAAAACGGGAACCAGCCTGCGTGGCAAGTCGTTGCATTATTGCAACCACTACGCCCGCGACGAGAGAATCGATATAGCgta                                                                                                                 >  2:1128091/1‑148 (MQ=255)
                                      agttgcagttgTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACCCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACgg                                                                                                             >  2:362465/1‑150 (MQ=255)
                                            agttgTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCAcc                                                                                                       <  2:659623/150‑1 (MQ=255)
                                               tgttTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCgag                                                                                                    >  2:872464/1‑150 (MQ=255)
                                                    tCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATg                                                                                               >  2:563769/1‑150 (MQ=255)
                                                    tCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATg                                                                                               >  1:1590587/1‑150 (MQ=255)
                                                     ccAAAAACTTTCCCCACGCGCGCCCATCTCGACCAACCGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATgg                                                                                              <  2:1044692/150‑1 (MQ=255)
                                                      cAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGc                                                                                             >  1:876148/1‑150 (MQ=255)
                                                      cAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAAAGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTACGCCCGCGCCGAGAGAATCGATATATCGTAAAGGTCCACCTAGAAATGGc                                                                                             >  2:1580641/1‑150 (MQ=255)
                                                      cAAAAACTTTCCCCACGCGCGCCCATCACGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGAAATATTGCAACCACATCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGc                                                                                             >  2:357248/1‑150 (MQ=255)
                                                       aaaaaCTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCg                                                                                            <  2:472616/150‑1 (MQ=255)
                                                        aaaaCTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCgg                                                                                           >  1:901847/1‑150 (MQ=255)
                                                              ttCCCCACGCGCGCCCATCTCGACCAAACACTCGCAAGGGGTAAAACGGGAACCATACAGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAAcc                                                                                     <  2:1095269/150‑1 (MQ=255)
                                                                   cACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGc                                                                                <  2:1285376/150‑1 (MQ=255)
                                                                     cgcgcgCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCa                                                                              <  1:750654/150‑1 (MQ=255)
                                                                       cgcgcCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCTGAAAACCAATGCCaaa                                                                            <  1:872464/150‑1 (MQ=255)
                                                                           cccATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGACCCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCCCCGAGAAATGGCGGAAAACCACTGCCAAATAcc                                                                        >  2:1292415/1‑150 (MQ=255)
                                                                           cccATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAAcc                                                                                     >  1:1463869/1‑137 (MQ=255)
                                                                           cccATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAAcc                                                                                     <  2:1463869/137‑1 (MQ=255)
                                                                           cccATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATAcc                                                                        <  1:1387613/150‑1 (MQ=255)
                                                                           cccATCTCGACCAAACGCTCGCAAGGGGAAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAGCCACTTCGCCCGCGCCGAGAGAATCGATATAGCCGAACGGTCCAACGACAAATGGCGCAAATCAAATGCCTAATAcc                                                                        >  2:249366/1‑150 (MQ=255)
                                                                               tctcGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCgcgc                                                                    <  2:1136595/150‑1 (MQ=255)
                                                                                  cGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCCCTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGACACCCAATGCCAAATACCGCGCCaa                                                                 >  2:916190/1‑150 (MQ=255)
                                                                                  cGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAACACCAATGCCAAATACCGCGCCaa                                                                 >  2:432354/1‑150 (MQ=255)
                                                                                  cGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCaa                                                                 >  1:555224/1‑150 (MQ=255)
                                                                                    aCCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAata                                                               >  1:680614/1‑150 (MQ=255)
                                                                                    aCCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAata                                                               >  2:373755/1‑150 (MQ=255)
                                                                                    aCCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAata                                                               <  2:1395457/150‑1 (MQ=255)
                                                                                          cGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCg                                                         <  2:541086/150‑1 (MQ=255)
                                                                                                    gggTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCacgcacgc                                               <  1:763876/150‑1 (MQ=255)
                                                                                                     ggTAAAACGGGTACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCt                                              >  1:1636647/1‑150 (MQ=255)
                                                                                                     ggTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCt                                              >  1:859119/1‑150 (MQ=255)
                                                                                                     ggTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCt                                              >  1:135048/1‑150 (MQ=255)
                                                                                                      gTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTa                                             >  1:810859/1‑150 (MQ=255)
                                                                                                           aCGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCCCTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGAt                                        >  1:1021772/1‑150 (MQ=255)
                                                                                                              ggAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAAc                                     >  2:1163269/1‑150 (MQ=255)
                                                                                                                 aCCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCCCCGAGAAAAGGCGGAAACCCACTGCCAATTACCGCGCCAATATCCCCGTCACGCCCGCTACGGagcgcctg                                  >  1:303250/1‑143 (MQ=255)
                                                                                                                  ccATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGc                                 >  2:165315/1‑150 (MQ=255)
                                                                                                                       cTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCtcatc                            <  1:1346391/150‑1 (MQ=255)
                                                                                                                         gCGTGTCAAGTCTTTGCATTATTGCAACCAATTCGCCCGCGCCGAGAGAATCGATATAGCGGAACTTTCCACCGAGAAATGTAGTAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCaa                          <  1:311821/150‑1 (MQ=255)
                                                                                                                           gTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAaca                        <  2:680614/150‑1 (MQ=255)
                                                                                                                           gTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAaca                        <  2:810859/150‑1 (MQ=255)
                                                                                                                             gcgaaGTGGTTGCAATAATGCAACGACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAACTGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCACGTCACGCACGCTACGGATAACCTGCTCATCAAcaca                      >  2:1533115/4‑150 (MQ=255)
                                                                                                                                aaGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACTGTCCACCGAGAAATGGCGGAAAAGCTATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACg                   <  1:1580641/150‑1 (MQ=255)
                                                                                                                                 aGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGTAAAACCAATGCCAAATACCGCGCCAATATCCCAGTCACGCACGCTTCGGATAACCTGCTCATCAACACATCGt                  <  1:1128091/150‑1 (MQ=255)
                                                                                                                                  gTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTa                 >  1:1514083/1‑150 (MQ=255)
                                                                                                                                     gTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGTGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTg              >  2:1567299/1‑150 (MQ=255)
                                                                                                                                       tGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACACCGTACTGCt            >  1:95456/1‑150 (MQ=255)
                                                                                                                                         caataatGCAACGACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGATTCGGATAACCTGCTCATCAACACAACGTACTGCttc          >  2:684678/7‑150 (MQ=255)
                                                                                                                                           ttattGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCttcat        >  1:77/1‑150 (MQ=255)
                                                                                                                                           ttattGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCttcat        >  2:180831/1‑150 (MQ=255)
                                                                                                                                            tattGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCttcatt       <  2:876148/150‑1 (MQ=255)
                                                                                                                                             attGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCttcattc      <  2:901847/150‑1 (MQ=255)
                                                                                                                                                 cAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCa  >  2:1083162/1‑150 (MQ=255)
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CGCGGATTCATATAGCTTTGACCTTCTTATTGCAGGTCAGTTGCAGTTGTTTTCCAAAAACTTTCCCCACGCGCGCCCATCTCGACCAAACGCTCGCAAGGGGTAAAACGGGAACCATACTGCGTGGCAAGTCGTTGCATTATTGCAACCACTTCGCCCGCGCCGAGAGAATCGATATAGCGGAACGGTCCACCGAGAAATGGCGGAAAACCAATGCCAAATACCGCGCCAATATCCCCGTCACGCACGCTACGGATAACCTGCTCATCAACACAACGTACTGCTTCATTCAGCA  >  NC_000913/2456989‑2457283

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: