Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,460,618 C→T 6.8% intergenic (‑149/+32) fadI ← / ← yfcZ beta‑ketoacyl‑CoA thiolase, anaerobic, subunit/UPF0381 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,460,6180CT 6.8% 373.4 / 9.6 120intergenic (‑149/+32)fadI/yfcZbeta‑ketoacyl‑CoA thiolase, anaerobic, subunit/UPF0381 family protein
Reads supporting (aligned to +/- strand):  ref base C (55/56);  new base T (5/3);  total (61/59)
Fisher's exact test for biased strand distribution p-value = 7.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.08e-01

Only 100 of 120 total aligned reads displayed.

AACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGT  >  NC_000913/2460470‑2460764
                                                                                                                                                    |                                                                                                                                                  
aaCACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCa                                                                                                                                                   <  2:1141456/150‑1 (MQ=255)
 acacTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCaa                                                                                                                                                  >  2:941090/1‑150 (MQ=255)
          tGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAggg                                                                                                                                         >  1:214226/1‑150 (MQ=255)
           taaaaaaaaaaaaaaaAGTGTTCTCACCTGAAAATAGACTAAACCATTTTTTAAAATTTAGCCAAGTGGACAAAAGGTAAAGTGGTAGATATGACACAGAGATAAAATAACAAGAGAAAAGAAAACGCCCCTGTCAGTTGCTGGCAgggg                                                                                                                                        <  2:350927/148‑1 (MQ=255)
           gTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAGACGCCCCTGCCAGCAACTGACAgggg                                                                                                                                        >  2:136431/1‑150 (MQ=255)
           gTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAgggg                                                                                                                                        <  1:950341/150‑1 (MQ=255)
             aaaaaaaTAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGcg                                                                                                                                      <  2:295066/150‑1 (MQ=255)
             aaaaaaaTAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTAACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGcg                                                                                                                                      >  1:1349291/1‑150 (MQ=255)
              aaaaaaTAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGcgt                                                                                                                                     <  1:1153137/150‑1 (MQ=255)
                aaaaTAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGcgtac                                                                                                                                   >  2:763297/1‑150 (MQ=255)
                 aaataaatAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGTCAGTTGCTGGCAGGGGcgtttt                                                                                                                                  <  2:592526/150‑4 (MQ=255)
                   ataaataaCTTGGTCTGACCTGCTCATAGTCTTAACCATTTTTTTACATTTCGCCAAGTGGAGAAACGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGcgtacgta                                                                                                                                <  2:1472143/150‑1 (MQ=255)
                   ataaataaAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGTCAGTTGCTGGCAGGGGcgttttct                                                                                                                                <  2:1009592/150‑6 (MQ=255)
                          aaGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCgaga                                                                                                                         >  1:629664/1‑150 (MQ=255)
                          aaGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCgaga                                                                                                                         >  2:1052568/1‑150 (MQ=255)
                           cggggTCTGACCTGATCATAGTATAAACCATTTTTTTAAATTAACCCAAGTGCAGAAAAGGGAACGTGGGAGCTATCACACAGAGAGAAAAGACCAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGagag                                                                                                                        <  1:879639/147‑1 (MQ=255)
                           agaggTCTGACCTGATCATAGTCTTATCCATTTTTTTACATTTAGCCAAGTGGAGAAAATGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTAGCCTagag                                                                                                                        <  2:94294/147‑1 (MQ=255)
                           aGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGagag                                                                                                                        <  1:255605/150‑1 (MQ=255)
                                 cTGACCTGATCTTATTCTTAACCATTTTTATACATTTAGGCAAGTGGAGAAAAGGGAAAGTGGGTGCAATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTa                                                                                                                  <  2:1257424/150‑1 (MQ=255)
                                 cTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTa                                                                                                                  >  2:1211432/1‑150 (MQ=255)
                                  tGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTaa                                                                                                                 <  1:961524/150‑1 (MQ=255)
                                   taCCTGATCAATGTCTAAACCATTTTTTTACATTTAGCCACGGGTAGAAAAGGTAAAGGGGTCGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAAc                                                                                                                <  1:879588/149‑1 (MQ=255)
                                    aCCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACg                                                                                                               >  2:1513881/1‑150 (MQ=255)
                                      cTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCa                                                                                                             <  2:503985/150‑1 (MQ=255)
                                      cTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGCGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCa                                                                                                             >  1:546502/1‑150 (MQ=255)
                                      cTGATCATAGTCTTAACAATTTTTTTACATTTAGCCACGTGTAGAAAAGGGAATTTGGGAGCTATGACACAGAGAGAAAAGAAGAAGATAAAAGAAAACGCCACTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCa                                                                                                             <  2:810533/150‑1 (MQ=255)
                                       tGATCTAAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAATAGGGAAAGTGGGATCTATGAAACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAg                                                                                                            <  1:837376/150‑1 (MQ=255)
                                       tGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGccg                                                                                                            >  2:1285298/1‑148 (MQ=255)
                                        gATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGa                                                                                                           >  1:1009845/1‑150 (MQ=255)
                                        gATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGa                                                                                                           >  2:1368478/1‑150 (MQ=255)
                                         aTCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGAc                                                                                                          <  2:214226/150‑1 (MQ=255)
                                          tCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGAcc                                                                                                         >  2:1114664/1‑150 (MQ=255)
                                           cATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACTCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGAccc                                                                                                        >  2:297321/1‑150 (MQ=255)
                                           aattttCTTAACCTTTTTTTTAAATTTAGCAAATTGTATAAAAGGGAAAGTGGTAGCTATGACACAGATATAAAATAATAAGATAAAATAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGAccc                                                                                                        <  2:1661975/145‑1 (MQ=255)
                                            aTAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCa                                                                                                       >  2:1471384/1‑150 (MQ=255)
                                               gTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCt                                                                                                    <  1:763297/150‑1 (MQ=255)
                                                   tAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGCCAGGGCCGTACGTACACGAGAGGAATTAACGCAGACCCAGCTGGaa                                                                                                >  1:241202/1‑150 (MQ=255)
                                                   tAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGaa                                                                                                >  1:183424/1‑150 (MQ=255)
                                                    aaCCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATCAACGCAGACCCAGCTGGaaa                                                                                               >  1:1674627/1‑150 (MQ=255)
                                                     aCCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGaaaa                                                                                              >  1:1688332/1‑150 (MQ=255)
                                                     aCCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGaaaa                                                                                              >  2:1449969/1‑150 (MQ=255)
                                                      ccATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAAt                                                                                             >  1:108231/1‑150 (MQ=255)
                                                      ccATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCACTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAAt                                                                                             >  2:1540408/1‑150 (MQ=255)
                                                       cATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATc                                                                                            >  1:311028/1‑150 (MQ=255)
                                                       cATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATc                                                                                            >  1:238943/1‑150 (MQ=255)
                                                          ttttttACATTTAGCCATGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAATAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGc                                                                                         <  2:1718774/150‑1 (MQ=255)
                                                          tttttaAAATTTAGCCAATTGGATAAAAGGTAAAGTGGGAGCTATGACACATATATAAAATAAGAACACAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGc                                                                                         <  1:1437861/150‑1 (MQ=255)
                                                            ttttACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACTGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCAt                                                                                       <  2:776930/150‑1 (MQ=255)
                                                                 cATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGCCCCAGCTGGAAAATCAGCATTTCAg                                                                                  >  1:1041783/1‑150 (MQ=255)
                                                                 cATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAg                                                                                  >  2:1124478/1‑150 (MQ=255)
                                                                  aTTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGc                                                                                 <  1:588270/150‑1 (MQ=255)
                                                                      aGCCTAGTGGAGAAACGGGACGGTGGGAGCTATCACACAGAGAGAACACAAGACGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCg                                                                             <  1:585149/150‑1 (MQ=255)
                                                                       gCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGc                                                                            <  1:941090/150‑1 (MQ=255)
                                                                           aGTGTATAAAAGGTAAAGTGGTAGCAATGACACTCAGAGAAAAGAAGAAGATAAAAGAACACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGc                                                                        <  2:1551690/150‑1 (MQ=255)
                                                                              ggAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCaaa                                                                     >  2:1467446/1‑150 (MQ=255)
                                                                               gagaAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCATCAATTCAGCTTCCCAGGCaaaa                                                                    >  1:839359/1‑150 (MQ=255)
                                                                               gagaAAAGGGAAAGTGCGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATATCAGCTTCGCAGGCaaaa                                                                    >  1:1136402/1‑150 (MQ=255)
                                                                                 gaAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGt                                                                  >  1:819777/1‑150 (MQ=255)
                                                                                  aaaaGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTg                                                                 <  1:1021293/150‑1 (MQ=255)
                                                                                     aGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAAAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCTTTTCAGCTTCGCAGGCAAAAGTGaaa                                                              >  2:1419769/1‑150 (MQ=255)
                                                                                       ggAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGCCCCAGCAGGAAAATCAGCATTTCAGCGTCGCAGGCAAAAGTGAAATc                                                            >  1:1063753/1‑150 (MQ=255)
                                                                                        gAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCg                                                           <  2:1054679/150‑1 (MQ=255)
                                                                                           attGGGAGATATTACAAATATATAAACGAAGAAGATAAAATAAAAAGCCCCTGCCAGCAACTGACAGGGGTGTACGTAACCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGata                                                        <  1:522701/148‑1 (MQ=255)
                                                                                           aGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTAAGCTTCGCAGGCAAAAGTGAAATCGata                                                        >  2:192735/1‑150 (MQ=255)
                                                                                              gggAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGCCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATcc                                                     >  2:844939/1‑150 (MQ=255)
                                                                                              gggAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATcc                                                     <  1:1114664/150‑1 (MQ=255)
                                                                                                 aGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAgg                                                  <  2:981288/150‑1 (MQ=255)
                                                                                                   cTATGACACAGAGAGAAAAGAAGAAGAGAAACGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGgcg                                                <  2:826980/150‑1 (MQ=255)
                                                                                                   cTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGgcg                                                <  1:414171/150‑1 (MQ=255)
                                                                                                    tATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGgcgc                                               >  2:1008711/1‑150 (MQ=255)
                                                                                                    tATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACAGACAGGGGCGTACGTACCCGAGAGCAATTAACGCAGACCCAGCTGGCAAATCAGCATATCACCATCGCAGGAAAAAGTGAAATCGATATCCAGgcgc                                               >  2:1002264/1‑150 (MQ=255)
                                                                                                    aaTGACAAAGAGAGAATAGAAGAAGAGAAAAGAAAACGGCCCTGCCAGCAACTGACAGGGGCGTACGAACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGgcgc                                               <  2:831031/149‑1 (MQ=255)
                                                                                                         cacaGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCAGTTCAGCTTCTCAGGCAAAAGTGAAATCGATATCCAGGCGCACAcc                                          >  1:342109/1‑150 (MQ=255)
                                                                                                           caGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCCGCTTCGCAGGCAAAAGTGAAATCGATTTCCAGGCGCACACCGt                                        >  1:875278/1‑150 (MQ=255)
                                                                                                             gagagaAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCa                                      <  2:684775/150‑1 (MQ=255)
                                                                                                              agagaaaagaagaagagaaaagaagaCGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAg                                     <  1:136431/150‑1 (MQ=255)
                                                                                                              agagaAATGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAg                                     <  1:432721/150‑1 (MQ=255)
                                                                                                               gagaAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGTGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGa                                    <  2:823064/150‑1 (MQ=255)
                                                                                                                agaAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGAc                                   <  1:864389/150‑1 (MQ=255)
                                                                                                                 gaAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGAct                                  >  1:710565/1‑150 (MQ=255)
                                                                                                                 gaAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGAct                                  <  2:238943/150‑1 (MQ=255)
                                                                                                                 gaAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGAct                                  >  2:982299/1‑150 (MQ=255)
                                                                                                                     agaagaagaGAAAAGAAAACGCCCCTGCCAGCAACTGCCAGGGGCGTACGTACCCGAGAGGAATAAACGCCGCCCCAGCTGGAAAATCAGCATTTCAGGTGCGCAGGCAAAAGTGACATCGATATCCAGACGCACACCGTCAGACTCTTc                              >  1:87275/1‑150 (MQ=255)
                                                                                                                     agaagaagaGAAAAGAAAACGCCCCTGCCAGCAACTGACAGTGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTc                              <  2:370528/150‑1 (MQ=255)
                                                                                                                        agaagaGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGt                           <  1:971345/150‑1 (MQ=255)
                                                                                                                                aaagaaaaCGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCg                   <  2:59036/150‑1 (MQ=255)
                                                                                                                                 aagaaaaCGCCCCTGCCAGCAACTGACAGGGGCGTACGTACACGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCgg                  >  2:62550/1‑150 (MQ=255)
                                                                                                                                      aaCGCCCCTGCCCGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCAGGAAAATCAGCATTACAGCTACGCAGGAAAAAGTGAGATCGATATCGAGGCGCACACGGTCAGCCTCTGCAGTGAAAGTCGGGGTGa             >  1:89758/1‑150 (MQ=255)
                                                                                                                                        cGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGAtt           >  1:448073/1‑150 (MQ=255)
                                                                                                                                        cGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGAtt           >  2:821126/1‑150 (MQ=255)
                                                                                                                                          ccccTGTCAGTTGCTGGCAGGGGCGTACGTACCCGAGAGGAATTACCGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGagtgt         >  1:244624/1‑146 (MQ=255)
                                                                                                                                          ccccTGTCAGTTGCTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGTATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGAtttt         >  2:1448377/1‑150 (MQ=255)
                                                                                                                                          ccccTGTCAGTTGCTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGAtttt         >  1:1265143/1‑150 (MQ=255)
                                                                                                                                          ccccTGTCAGTTGCTGGCAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCGTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTGAGTGAAAGTCGGGGTGAtttt         >  1:1634396/1‑150 (MQ=255)
                                                                                                                                          ccccTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGAtttt         >  1:1241775/1‑150 (MQ=255)
                                                                                                                                              tGCCAGCTACTGTCAGGGTCGTACGTTCCCGAGAGGATTTAACGGAGACCCTGCTGGATAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAATTCGATATCCAGGCGCACACCGTCTGACTCTTCAGTGAAAGTCGGGGTGATTTTGCaa     <  1:1002264/150‑1 (MQ=255)
                                                                                                                                              tGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCaa     <  1:1008711/150‑1 (MQ=255)
                                                                                                                                               gCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAg    <  1:1368478/150‑1 (MQ=255)
                                                                                                                                                 cAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACCCCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGt  >  2:985722/1‑150 (MQ=255)
                                                                                                                                                 cAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGt  <  2:108231/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
AACACTCCCCTGTAAAAAAATAAATAAAGTGGTCTGACCTGATCATAGTCTTAACCATTTTTTTACATTTAGCCAAGTGGAGAAAAGGGAAAGTGGGAGCTATGACACAGAGAGAAAAGAAGAAGAGAAAAGAAAACGCCCCTGCCAGCAACTGACAGGGGCGTACGTACCCGAGAGGAATTAACGCAGACCCAGCTGGAAAATCAGCATTTCAGCTTCGCAGGCAAAAGTGAAATCGATATCCAGGCGCACACCGTCAGACTCTTCAGTGAAAGTCGGGGTGATTTTGCAAGGT  >  NC_000913/2460470‑2460764

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: