Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,464,224 T→C 8.1% intergenic (+154/+28) yfdF → / ← mlaA uncharacterized protein/ABC transporter maintaining OM lipid asymmetry, OM lipoprotein component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,464,2240TC 8.1% 276.3 / 12.3 99intergenic (+154/+28)yfdF/mlaAuncharacterized protein/ABC transporter maintaining OM lipid asymmetry, OM lipoprotein component
Reads supporting (aligned to +/- strand):  ref base T (50/41);  new base C (5/3);  total (55/44)
Fisher's exact test for biased strand distribution p-value = 7.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.76e-01

Only 100 of 105 total aligned reads displayed.

ATTTTTCCAGTTTTTTGATTTTTCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAGTACGCTTCGCGCACC  >  NC_000913/2464075‑2464372
                                                                                                                                                     |                                                                                                                                                    
attTTTCCAGTTTTTTGATTTTTCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGtt                                                                                                                                                      <  1:1594308/150‑1 (MQ=255)
attTTTCCAGTTTTTTGATTTTTCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGtt                                                                                                                                                      <  1:1409649/150‑1 (MQ=255)
attTTTCCAGTTTTTTGATTTTTCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGtt                                                                                                                                                      >  2:391018/1‑150 (MQ=255)
   tttCCAGTTTTTTGATTTTTCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTg                                                                                                                                                   <  2:584637/150‑1 (MQ=255)
    ttCCAGTTTTTTGATTTTTCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGc                                                                                                                                                  <  1:430106/150‑1 (MQ=255)
     tCCAGTTTTTTGATTTTTCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCg                                                                                                                                                 >  1:1145639/1‑150 (MQ=255)
          ttttttGATTTTTCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCa                                                                                                                                            >  1:781982/1‑150 (MQ=255)
                taTTTTTCATCAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTTGGTCATTTTCGGCTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCtttt                                                                                                                                      <  2:1494263/149‑1 (MQ=255)
                taTTTTTCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACAAAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCtttt                                                                                                                                      <  2:806401/149‑1 (MQ=255)
                gATTTTTCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCtttt                                                                                                                                      >  2:1374413/1‑150 (MQ=255)
                 aTTTTTCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTCGCAAAACTCACCttttt                                                                                                                                     <  1:1117178/150‑1 (MQ=255)
                  tttttCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTCGCAAAACTCACCtttttt                                                                                                                                    <  2:1063415/150‑1 (MQ=255)
                    tttCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTCGCAAAACTCACCttttttgt                                                                                                                                  <  2:371887/150‑3 (MQ=255)
                     ttCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTAtt                                                                                                                                 <  1:1304968/150‑1 (MQ=255)
                      tCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTCAGTTTAGATACTCACCTTTTTTAttt                                                                                                                                >  2:110726/1‑150 (MQ=255)
                       cAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTg                                                                                                                               <  1:1249445/150‑1 (MQ=255)
                            gCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCg                                                                                                                                                 <  2:288643/127‑1 (MQ=255)
                            gCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCg                                                                                                                                                 >  1:288643/1‑127 (MQ=255)
                              tGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGTGATAACAACAAAAAAGTTGAGTTTTGCGACTCACATTTTTTATTTGTTTCTTa                                                                                                                        >  1:242265/1‑150 (MQ=255)
                                   atCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAg                                                                                                                   >  2:477471/1‑150 (MQ=255)
                                    tCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGa                                                                                                                  >  2:1018357/1‑150 (MQ=255)
                                                 gTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCtttt                                                                                                     >  2:692345/1‑150 (MQ=255)
                                                 gTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCtttt                                                                                                     >  2:1130563/1‑150 (MQ=255)
                                                   gACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTaa                                                                                                   <  1:801060/150‑1 (MQ=255)
                                                   gACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTaa                                                                                                   >  1:1303513/1‑150 (MQ=255)
                                                   gACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTaa                                                                                                   <  1:741343/150‑1 (MQ=255)
                                                   gACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTAAGAATCAATATCTTTTaa                                                                                                   >  2:769096/1‑150 (MQ=255)
                                                    aCGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTaaa                                                                                                  >  2:1284401/1‑150 (MQ=255)
                                                     cGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAat                                                                                                 >  2:388401/1‑150 (MQ=255)
                                                          aTCATTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGTCTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATcc                                                                                            <  1:1173678/150‑1 (MQ=255)
                                                          aTCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATcc                                                                                            <  2:886803/150‑1 (MQ=255)
                                                             aGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGa                                                                                         >  1:318116/1‑150 (MQ=255)
                                                             aGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGa                                                                                         >  2:372928/1‑150 (MQ=255)
                                                             aGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGa                                                                                         <  2:424087/150‑1 (MQ=255)
                                                              gTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGaa                                                                                        >  2:1572687/1‑150 (MQ=255)
                                                                   tttACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCt                                                                                   <  1:1215328/150‑1 (MQ=255)
                                                                   tttACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCt                                                                                   <  1:692345/150‑1 (MQ=255)
                                                                    ttACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAAAAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCtt                                                                                  <  1:845027/150‑1 (MQ=255)
                                                                     tACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTtg                                                                                 <  2:781982/150‑1 (MQ=255)
                                                                     tACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTtg                                                                                 <  1:1304467/150‑1 (MQ=255)
                                                                      aCATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTtgt                                                                                >  2:611106/1‑150 (MQ=255)
                                                                        aTAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGc                                                                              >  2:582034/1‑150 (MQ=255)
                                                                         tAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCg                                                                             <  1:480765/150‑1 (MQ=255)
                                                                           aTTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGtt                                                                           >  1:52022/1‑150 (MQ=255)
                                                                             tACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCg                                                                         <  1:880569/150‑1 (MQ=255)
                                                                              aCGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGAGTTCgt                                                                        >  1:235207/1‑149 (MQ=255)
                                                                                  cTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGAttt                                                                    >  2:37754/1‑150 (MQ=255)
                                                                                    aGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTTTTTCTTATTAAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCTTTCGGATTTTc                                                                  >  1:1293106/1‑150 (MQ=255)
                                                                                    aGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTc                                                                  >  1:1119110/1‑150 (MQ=255)
                                                                                    aGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGATTGTGCGTTCGGATTTTc                                                                  >  2:1267441/1‑150 (MQ=255)
                                                                                    aGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATACTGAATCGCTTGTGCGTTCGGATTTTc                                                                  >  1:534606/1‑150 (MQ=255)
                                                                                           tGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGt                                                           <  2:151526/150‑1 (MQ=255)
                                                                                             tCGGTTTATCTGTTTTTAATTGTGGTTATTGAGTGATATCAATAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGttt                                                         <  1:110726/150‑1 (MQ=255)
                                                                                               ggTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGa                                                       >  1:1660721/1‑150 (MQ=255)
                                                                                               ggTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGa                                                       <  2:36284/150‑1 (MQ=255)
                                                                                               ggTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGa                                                       >  1:100519/1‑150 (MQ=255)
                                                                                                   tATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGtta                                                   >  1:1303769/1‑149 (MQ=255)
                                                                                                     tctcCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATAAATATCTTTTAAATCATCATGAATCGCGTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTcgc                                                 >  2:256771/1‑150 (MQ=255)
                                                                                                       tcCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTcgccg                                               >  2:1547512/1‑150 (MQ=255)
                                                                                                       tcCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTcgccg                                               <  2:1660721/150‑1 (MQ=255)
                                                                                                        ccTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTcgccgc                                              <  1:391018/150‑1 (MQ=255)
                                                                                                          ttttACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCa                                            <  2:1660337/150‑1 (MQ=255)
                                                                                                            ttACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCAtt                                          >  1:600780/1‑150 (MQ=255)
                                                                                                             tACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTa                                         >  1:393504/1‑150 (MQ=255)
                                                                                                                ttGTGGTAACTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCGGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCg                                      <  2:1163257/150‑1 (MQ=255)
                                                                                                                 tgtgGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGa                                     >  1:1695103/1‑150 (MQ=255)
                                                                                                                 tgtgGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCATGCGGTTTGAGTTCGCCGCCATTAGCGa                                     >  2:644529/1‑150 (MQ=255)
                                                                                                                  gtgGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGAt                                    >  1:1409334/1‑150 (MQ=255)
                                                                                                                    ggTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGa                                  <  1:37754/150‑1 (MQ=255)
                                                                                                                     gTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGAAGCCATTAGCGATGaa                                 >  2:831439/1‑150 (MQ=255)
                                                                                                                      tAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGaaa                                >  2:1331238/1‑150 (MQ=255)
                                                                                                                        aTTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATc                              <  2:52022/150‑1 (MQ=255)
                                                                                                                         ttGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCa                             <  2:1286430/150‑1 (MQ=255)
                                                                                                                          tGAGGGATAACAACAAAAAAGGTGAGTTTTGCGCCTCACATTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGATTGAGTTCGCAGCCATTAGCGATTAAATCAt                            >  1:1316270/1‑150 (MQ=255)
                                                                                                                          tGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTcc                                                                 <  2:714129/113‑1 (MQ=255)
                                                                                                                          tGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTcc                                                                 >  1:714129/1‑113 (MQ=255)
                                                                                                                            aGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGa                          >  1:477288/1‑150 (MQ=255)
                                                                                                                               gATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCAGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGc                       <  2:722649/150‑1 (MQ=255)
                                                                                                                                aTAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCt                      <  2:1433710/150‑1 (MQ=255)
                                                                                                                                 tAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTg                     >  2:1425745/1‑150 (MQ=255)
                                                                                                                                 tAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTg                     <  1:407906/150‑1 (MQ=255)
                                                                                                                                   caaataaaaaaGGTGAGTCGCAAAACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGa                   >  1:800198/6‑150 (MQ=38)
                                                                                                                                   acaacaAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAACTCATCCTGAATCGCTGGTGCGTTCGGATTTTCCTGCGGTTTGAGTGCGCCGCCATTAGCGATGAAATCATGACGCTGGa                   <  2:1527158/150‑1 (MQ=255)
                                                                                                                                   acaacaAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGa                   >  1:651145/1‑150 (MQ=255)
                                                                                                                                    caacaaAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGaa                  <  1:388401/150‑1 (MQ=255)
                                                                                                                                    aaataaaaaaGGTGAGTCGCAAAACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGaa                  >  2:1363775/5‑150 (MQ=39)
                                                                                                                                     aataaaaaaGGTGAGTCGCAAAACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAg                 >  1:10108/4‑150 (MQ=255)
                                                                                                                                     aataaaaaaGGTGAGTCGCAAAACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAg                 >  1:3945/4‑150 (MQ=255)
                                                                                                                                     aacaaAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAg                 <  2:1145639/150‑1 (MQ=255)
                                                                                                                                     aacaaAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCACAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTACCAGCGGTTTGAGTTCGCCGCCATTAGAGATGAAATCATGACGCTGGAAg                 >  1:1473326/1‑150 (MQ=255)
                                                                                                                                         aaaaaGGTGCGTTTTGCGACTCACCTTTTTTATTTGTTTGTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGACATCATGACGCTGGAAGTACg             >  1:64350/1‑150 (MQ=255)
                                                                                                                                         aaaaaGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAGTACg             >  1:769211/1‑150 (MQ=255)
                                                                                                                                           aaaGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGAGTTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAGTACGCt           >  2:620874/1‑150 (MQ=255)
                                                                                                                                             aGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAGTACGCTTc         <  1:982028/150‑1 (MQ=255)
                                                                                                                                               gTGAGTTTTGGGACTCACCTTTTTTATTTGTTTATTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAGTACGCTTcgc       <  2:242265/150‑1 (MQ=255)
                                                                                                                                               gTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAGTACGCTTcgc       <  1:1130563/150‑1 (MQ=255)
                                                                                                                                                tGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAGTACGCTTcgcg      <  1:946935/150‑1 (MQ=255)
                                                                                                                                                    ttttGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAGTACGCTTCGCGCAcc  <  1:1374413/150‑1 (MQ=255)
                                                                                                                                                    ttttGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAGTACGCTTCGCGCAcc  <  1:379693/150‑1 (MQ=255)
                                                                                                                                                    ttttGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGGTTGAGTTCGCCGCCATTAGCGAGGAAATCATGACGCTGGAAGTACGCTTCGCGcccc  <  1:379690/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
ATTTTTCCAGTTTTTTGATTTTTCAACAGCTGAATATCGGAAATAGTCAGTGACGAACATCAGTATTTTTACATAATTACGTCTAGGTCATTGTCGGTTTATCTCCTTTTACTTGTGGTAATTGAGGGATAACAACAAAAAAGGTGAGTTTTGCGACTCACCTTTTTTATTTGTTTCTTATTCAGAATCAATATCTTTTAAATCATCCTGAATCGCTTGTGCGTTCGGATTTTCCTGCGGTTTGAGTTCGCCGCCATTAGCGATGAAATCATGACGCTGGAAGTACGCTTCGCGCACC  >  NC_000913/2464075‑2464372

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: