Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,473,468 T→C 5.2% pseudogene (118/708 nt) oweS ← pseudogene, CPS‑53 (KpLE1) prophage; bacteriophage replication protein O family;Phage or Prophage Related

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,473,4680TC 5.2% 287.5 / 4.8 96pseudogene (118/708 nt)oweSpseudogene, CPS‑53 (KpLE1) prophage; bacteriophage replication protein O family;Phage or Prophage Related
Reads supporting (aligned to +/- strand):  ref base T (38/53);  new base C (3/2);  total (41/55)
Fisher's exact test for biased strand distribution p-value = 6.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01

CTGTTCATCCGTCTGCGTGTCCGGTTGCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGCGGTTATGATGAGGAGCAGCAAAATGGCTAG  >  NC_000913/2473319‑2473611
                                                                                                                                                     |                                                                                                                                               
cTGTTCATCCGTCTGCGTGTCCGGTTGCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAAttt                                                                                                                                                 >  1:1168268/1‑150 (MQ=255)
 tGTTCATCCGTCTGCGTGTCCGGTTGCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAAtttt                                                                                                                                                >  2:863143/1‑150 (MQ=255)
  gTTCATCCGTCTGCGTGTCCGGTTGCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTg                                                                                                                                               <  2:730491/150‑1 (MQ=255)
   ttCATCCGTCTGCGTGTCCGGTTGCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTGATTTTACTGACGGATCACCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGa                                                                                                                                              <  2:1354965/150‑1 (MQ=255)
    tCATCCGTCTGCGTGTCCGGTTGCGAAGCGTCCGGACGAGAAGGTTTTTTATCTTACGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGAc                                                                                                                                             <  2:234164/150‑1 (MQ=255)
     cATCCGTCTGCGTGTCCGGTTGCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACg                                                                                                                                            >  2:763899/1‑150 (MQ=255)
     cATCCGTCTGCGTGTCCGGTTGCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACg                                                                                                                                            >  1:1540733/1‑150 (MQ=255)
          gTCTGCGTGTCCGGTTGCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCa                                                                                                                                       >  1:639600/1‑150 (MQ=255)
           tCTGCGTGTCCGGTTGCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAg                                                                                                                                      <  2:1620889/150‑1 (MQ=255)
            cTGCGTGTCCGGTTGCGCAGCGTCCGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGATAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGa                                                                                                                                     >  1:827850/1‑150 (MQ=255)
                gtgtCCGGTTGCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGAtttt                                                                                                                                 >  1:366919/1‑150 (MQ=255)
                 tgtCCGGTTGCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTg                                                                                                                                <  2:458406/150‑1 (MQ=255)
                  gtCCGGTTGCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGa                                                                                                                               <  1:1100474/150‑1 (MQ=255)
                   tCCGGTTGCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGAt                                                                                                                              >  2:240960/1‑150 (MQ=255)
                    ccGGTTGCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGAGGGGTCAGATTTTGATg                                                                                                                             >  1:998031/1‑150 (MQ=255)
                       gTTGCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAAATCTGACCCGTCAAAATTTGATGCGt                                                                                                                          <  1:1696868/150‑1 (MQ=39)
                        ttGCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTc                                                                                                                         <  2:1168268/150‑1 (MQ=255)
                        ttGCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAAATCTGACCCGTCAAAATTTGATGCGTc                                                                                                                         <  1:1049346/150‑1 (MQ=39)
                         tGCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCa                                                                                                                        >  2:376673/1‑150 (MQ=255)
                         tGCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCa                                                                                                                        >  2:1156509/1‑150 (MQ=255)
                          gCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCTGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAg                                                                                                                       <  1:825123/150‑1 (MQ=255)
                          gCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAg                                                                                                                       <  1:863143/150‑1 (MQ=255)
                          gCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAg                                                                                                                       >  2:1334321/1‑150 (MQ=255)
                                cGTCCGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGCATCAAAATCTGACCCGTCAAAATTTg                                                                                                                 <  2:920163/150‑1 (MQ=255)
                                 gTCCGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGa                                                                                                                <  2:178003/150‑1 (MQ=255)
                                  tCCGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGAc                                                                                                               <  2:1528163/150‑1 (MQ=255)
                                   ccGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACg                                                                                                              <  2:991174/150‑1 (MQ=255)
                                     ggACGAGAAGGTTTTTTATCTGACGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACggg                                                                                                            <  1:140789/150‑1 (MQ=255)
                                          agaAGGTTTTTTATCTGCCGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATCCGTCAGATTTTGACGGGTCaga                                                                                                       >  2:134681/1‑150 (MQ=255)
                                          agaAGGTTTTTTATCTGACGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCaga                                                                                                       >  2:1211292/1‑150 (MQ=255)
                                          agaAGGTTTTTTATCTGACGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCaga                                                                                                       >  2:60092/1‑150 (MQ=255)
                                              ggTTTTTTATCTGACGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCt                                                                                                   <  2:1349846/150‑1 (MQ=255)
                                              ggTTTTTTATCTGACGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTAGCCAGATTTCGACGCATCAATTTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCt                                                                                                   <  1:711475/150‑1 (MQ=255)
                                               gTTTTTTATCTGACGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTg                                                                                                  <  1:1339445/150‑1 (MQ=255)
                                                  ttttATCTGACGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACa                                                                                               <  2:1121026/150‑1 (MQ=255)
                                                      aTCTGACGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTg                                                                                           <  2:778461/150‑1 (MQ=255)
                                                          gACGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGaa                                                                                       <  1:1033043/150‑1 (MQ=255)
                                                          gACGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGaa                                                                                       >  1:274644/1‑150 (MQ=255)
                                                          gACGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGaa                                                                                       <  1:46010/150‑1 (MQ=255)
                                                           aCGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGaaa                                                                                      >  1:710157/1‑150 (MQ=255)
                                                           aCGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGaaa                                                                                      <  1:369079/150‑1 (MQ=255)
                                                              gATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTTAGAAAATg                                                                                   >  2:606083/1‑150 (MQ=255)
                                                              gATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATg                                                                                   <  2:1229221/150‑1 (MQ=255)
                                                                tCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGcc                                                                                 <  1:1123786/150‑1 (MQ=255)
                                                                 cATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCg                                                                                <  1:427531/150‑1 (MQ=255)
                                                                  aTGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGc                                                                               <  2:215643/150‑1 (MQ=255)
                                                                   tgatttGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGGCAGAGTCTGACAGTTGAGAAAATGCCGCt                                                                              <  2:232895/147‑1 (MQ=255)
                                                                     ttttGATTATACTGACGTATCCCCTCCATATTCTGACGTGTGATAAACCGATTTTTTGCCAGTTTTCGACGCTTCAAATTTTGACGGGTCAGATTTTGATGCGTCAGTTTTTGACGGGTCAGAGTCTGACAGTTGAGTAAATGCCGCTGc                                                                            <  1:1148603/150‑1 (MQ=255)
                                                                      tttGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGcc                                                                           >  2:417433/1‑150 (MQ=255)
                                                                      tttGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGcc                                                                           >  2:16444/1‑150 (MQ=255)
                                                                        tGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTg                                                                         <  1:1211292/150‑1 (MQ=255)
                                                                         gATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGa                                                                        <  2:189691/150‑1 (MQ=255)
                                                                                  gACGGATCCCCGCCAGATTCTGACGGGTGAACACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCa                                                               >  2:263799/1‑150 (MQ=255)
                                                                                    cGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAAc                                                             >  1:693423/1‑150 (MQ=255)
                                                                                    cGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAAc                                                             >  2:886764/1‑150 (MQ=255)
                                                                                     ggATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACg                                                            <  1:1156509/150‑1 (MQ=255)
                                                                                     ggATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACg                                                            <  1:376673/150‑1 (MQ=255)
                                                                                       aTCCCCGCCAGATTCAGACGGGGGAAACCCCGATTTTTTGCCAGATTTCGACCCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGCTCAGAGTCTGACAGTTGAGATAATGCCGCTGCCTGAAGCTTCGCAACGtt                                                          <  2:1173573/150‑1 (MQ=255)
                                                                                         ccccGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTaa                                                        <  2:705111/150‑1 (MQ=255)
                                                                                           ccGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGc                                                      >  1:1151269/1‑150 (MQ=255)
                                                                                           ccGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGc                                                      <  2:1540733/150‑1 (MQ=255)
                                                                                                aGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATa                                                 >  1:1244725/1‑150 (MQ=255)
                                                                                                 gATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGAAGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATaa                                                <  1:834174/150‑1 (MQ=255)
                                                                                                    tCTGACGGGAGAAAACCCTATTTTTTGCCAGATTTCTACGCATCAAATTTTGAAGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACa                                             <  2:131705/150‑1 (MQ=255)
                                                                                                       gACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTc                                          <  2:466161/150‑1 (MQ=255)
                                                                                                          gggTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGAc                                       <  1:642991/150‑1 (MQ=255)
                                                                                                           ggTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACg                                      >  1:1472619/1‑150 (MQ=255)
                                                                                                               aaaaCCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCAtt                                  >  2:607484/1‑150 (MQ=255)
                                                                                                                aaaCCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTg                                 <  2:1244725/150‑1 (MQ=255)
                                                                                                                 aaCCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGc                                >  2:696135/1‑150 (MQ=255)
                                                                                                                 aaCCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGc                                >  2:702343/1‑150 (MQ=255)
                                                                                                                  aCCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGCg                               <  2:1071685/150‑1 (MQ=255)
                                                                                                                   cccGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGCgg                              >  1:711284/1‑150 (MQ=255)
                                                                                                                    ccGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGCGGt                             <  1:763899/150‑1 (MQ=255)
                                                                                                                       aTTTTTTGCCAGATTTCGACTCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGCGGTTat                          <  2:1189776/150‑1 (MQ=255)
                                                                                                                         tttttGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGGCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGCGGTTatga                        <  2:72514/150‑1 (MQ=255)
                                                                                                                            ttGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGCGGTTAtgatga                     <  1:1596099/150‑1 (MQ=255)
                                                                                                                              gCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGCGGTTATGATgagg                   >  2:1516850/1‑150 (MQ=255)
                                                                                                                              gCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGCGGTTATGATgagg                   <  2:510160/150‑1 (MQ=255)
                                                                                                                              gCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGCGGTTATGATgagg                   >  1:1325860/1‑150 (MQ=255)
                                                                                                                              gCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGAAGCATTGCGGTTATGATgagg                   >  2:915696/1‑150 (MQ=255)
                                                                                                                              gCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCAGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGCGGTTATGATgagg                   >  2:339140/1‑150 (MQ=255)
                                                                                                                               ccAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGCGGTTATGATgagga                  <  2:1110499/150‑1 (MQ=255)
                                                                                                                                 aGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGCGGTTATGATGAGGagc                <  2:639600/150‑1 (MQ=255)
                                                                                                                                   aTTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGAAAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGCGGTTATGATGAGGagcag              <  2:1493944/150‑1 (MQ=255)
                                                                                                                                    tttCGACGCATAAAATTTTGACGGGTCATATTTTGATGCGTCAGATTTTGACGGGTCAGATTCTGAAAGTTGATAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGCGGTTATGATGAGGagcagc             <  1:606083/150‑1 (MQ=255)
                                                                                                                                      ctgACGCATCAAAATCTGACCCGTCAAAATTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGCGGTTATGATGAGGAGCAGCaa           >  2:514738/3‑150 (MQ=38)
                                                                                                                                       tgACGCATCAAAATCTGACCCGTCAAAATTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGCGGTTATGATGAGGAGCAGCaaa          >  1:1441930/2‑150 (MQ=39)
                                                                                                                                        gACGCATCAAAATCTGACCCGTCAAAATTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGTGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGAGAAACATTCGACGCAGTGCGGTTAGGATGAGGAGCAGCaaaa         >  2:1203589/1‑150 (MQ=38)
                                                                                                                                          cGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTAATAAAAATTCGACGCAATGCGGTTATGATGAGGAGCAGCAAAATg       >  2:1702435/1‑150 (MQ=255)
                                                                                                                                           gCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGCCGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCTCAACGTTACGCTGATAAACATTCGACGCATTGCGGTTATGATGAGCAGCAGCTAAATgg      >  1:1063241/1‑150 (MQ=255)
                                                                                                                                           gCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGCGGTTATGATGAGGAGCAGCAAAATgg      <  1:417433/150‑1 (MQ=255)
                                                                                                                                            cATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGCGGTTATGATGAGGAGCAGCAAAATGGc     >  2:1616159/1‑150 (MQ=255)
                                                                                                                                              tCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGCGGTTATGATGAGGagcag              >  1:996716/1‑139 (MQ=255)
                                                                                                                                              tCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGCGGTTATGATGAGGagcag              <  2:996716/139‑1 (MQ=255)
                                                                                                                                              tCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGAGGTTATGATGAGGAGCAGCAAAATGGCTa   >  1:1136535/1‑150 (MQ=255)
                                                                                                                                               cAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGCGGTTATGATGAGGAGCAGCAAAATGGCTAg  <  1:16444/150‑1 (MQ=255)
                                                                                                                                               cAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGCGGTTATGATGAGGAGCAGCAAAATGGCTAg  >  2:956167/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                               
CTGTTCATCCGTCTGCGTGTCCGGTTGCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGCGGTTATGATGAGGAGCAGCAAAATGGCTAG  >  NC_000913/2473319‑2473611

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑