Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,473,479 G→A 6.3% pseudogene (107/708 nt) oweS ← pseudogene, CPS‑53 (KpLE1) prophage; bacteriophage replication protein O family;Phage or Prophage Related

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,473,4790GA 6.3% 301.8 / 5.7 96pseudogene (107/708 nt)oweSpseudogene, CPS‑53 (KpLE1) prophage; bacteriophage replication protein O family;Phage or Prophage Related
Reads supporting (aligned to +/- strand):  ref base G (38/51);  new base A (3/3);  total (41/55)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.83e-01

Only 100 of 101 total aligned reads displayed.

TCTGCGTGTCCGGTTGCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGCGGTTATGATGAGGAGCAGCAAAATGGCTAGCGAACGCAGTACTGATG  >  NC_000913/2473330‑2473628
                                                                                                                                                     |                                                                                                                                                     
tCTGCGTGTCCGGTTGCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAg                                                                                                                                                       <  2:1620889/150‑1 (MQ=255)
 cTGCGTGTCCGGTTGCGCAGCGTCCGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGATAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGa                                                                                                                                                      >  1:827850/1‑150 (MQ=255)
     gtgtCCGGTTGCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGAtttt                                                                                                                                                  >  1:366919/1‑150 (MQ=255)
      tgtCCGGTTGCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTg                                                                                                                                                 <  2:458406/150‑1 (MQ=255)
       gtCCGGTTGCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGa                                                                                                                                                <  1:1100474/150‑1 (MQ=255)
        tCCGGTTGCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGAt                                                                                                                                               >  2:240960/1‑150 (MQ=255)
         ccGGTTGCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGAGGGGTCAGATTTTGATg                                                                                                                                              >  1:998031/1‑150 (MQ=255)
            gTTGCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAAATCTGACCCGTCAAAATTTGATGCGt                                                                                                                                           <  1:1696868/150‑1 (MQ=39)
             ttGCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTc                                                                                                                                          <  2:1168268/150‑1 (MQ=255)
             ttGCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAAATCTGACCCGTCAAAATTTGATGCGTc                                                                                                                                          <  1:1049346/150‑1 (MQ=39)
              tGCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCa                                                                                                                                         >  2:1156509/1‑150 (MQ=255)
              tGCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCa                                                                                                                                         >  2:376673/1‑150 (MQ=255)
               gCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCTGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAg                                                                                                                                        <  1:825123/150‑1 (MQ=255)
               gCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAg                                                                                                                                        >  2:1334321/1‑150 (MQ=255)
               gCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAg                                                                                                                                        <  1:863143/150‑1 (MQ=255)
                     cGTCCGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGCATCAAAATCTGACCCGTCAAAATTTg                                                                                                                                  <  2:920163/150‑1 (MQ=255)
                      gTCCGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGa                                                                                                                                 <  2:178003/150‑1 (MQ=255)
                       tCCGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGAc                                                                                                                                <  2:1528163/150‑1 (MQ=255)
                        ccGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACg                                                                                                                               <  2:991174/150‑1 (MQ=255)
                          ggACGAGAAGGTTTTTTATCTGACGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACggg                                                                                                                             <  1:140789/150‑1 (MQ=255)
                               agaAGGTTTTTTATCTGCCGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATCCGTCAGATTTTGACGGGTCaga                                                                                                                        >  2:134681/1‑150 (MQ=255)
                               agaAGGTTTTTTATCTGACGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCaga                                                                                                                        >  2:1211292/1‑150 (MQ=255)
                               agaAGGTTTTTTATCTGACGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCaga                                                                                                                        >  2:60092/1‑150 (MQ=255)
                                   ggTTTTTTATCTGACGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCt                                                                                                                    <  2:1349846/150‑1 (MQ=255)
                                   ggTTTTTTATCTGACGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTAGCCAGATTTCGACGCATCAATTTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCt                                                                                                                    <  1:711475/150‑1 (MQ=255)
                                    gTTTTTTATCTGACGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTg                                                                                                                   <  1:1339445/150‑1 (MQ=255)
                                       ttttATCTGACGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACa                                                                                                                <  2:1121026/150‑1 (MQ=255)
                                           aTCTGACGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTg                                                                                                            <  2:778461/150‑1 (MQ=255)
                                               gACGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGaa                                                                                                        <  1:1033043/150‑1 (MQ=255)
                                               gACGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGaa                                                                                                        >  1:274644/1‑150 (MQ=255)
                                               gACGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGaa                                                                                                        <  1:46010/150‑1 (MQ=255)
                                                aCGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGaaa                                                                                                       >  1:710157/1‑150 (MQ=255)
                                                aCGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGaaa                                                                                                       <  1:369079/150‑1 (MQ=255)
                                                   gATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTTAGAAAATg                                                                                                    >  2:606083/1‑150 (MQ=255)
                                                   gATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATg                                                                                                    <  2:1229221/150‑1 (MQ=255)
                                                     tCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGcc                                                                                                  <  1:1123786/150‑1 (MQ=255)
                                                      cATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCg                                                                                                 <  1:427531/150‑1 (MQ=255)
                                                       aTGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGc                                                                                                <  2:215643/150‑1 (MQ=255)
                                                        tgatttGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGGCAGAGTCTGACAGTTGAGAAAATGCCGCt                                                                                               <  2:232895/147‑1 (MQ=255)
                                                          ttttGATTATACTGACGTATCCCCTCCATATTCTGACGTGTGATAAACCGATTTTTTGCCAGTTTTCGACGCTTCAAATTTTGACGGGTCAGATTTTGATGCGTCAGTTTTTGACGGGTCAGAGTCTGACAGTTGAGTAAATGCCGCTGc                                                                                             <  1:1148603/150‑1 (MQ=255)
                                                           tttGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGcc                                                                                            >  2:16444/1‑150 (MQ=255)
                                                           tttGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGcc                                                                                            >  2:417433/1‑150 (MQ=255)
                                                             tGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTg                                                                                          <  1:1211292/150‑1 (MQ=255)
                                                              gATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGa                                                                                         <  2:189691/150‑1 (MQ=255)
                                                                       gACGGATCCCCGCCAGATTCTGACGGGTGAACACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCa                                                                                >  2:263799/1‑150 (MQ=255)
                                                                         cGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAAc                                                                              >  1:693423/1‑150 (MQ=255)
                                                                         cGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAAc                                                                              >  2:886764/1‑150 (MQ=255)
                                                                          ggATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACg                                                                             <  1:376673/150‑1 (MQ=255)
                                                                          ggATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACg                                                                             <  1:1156509/150‑1 (MQ=255)
                                                                            aTCCCCGCCAGATTCAGACGGGGGAAACCCCGATTTTTTGCCAGATTTCGACCCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGCTCAGAGTCTGACAGTTGAGATAATGCCGCTGCCTGAAGCTTCGCAACGtt                                                                           <  2:1173573/150‑1 (MQ=255)
                                                                              ccccGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTaa                                                                         <  2:705111/150‑1 (MQ=255)
                                                                                ccGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGc                                                                       <  2:1540733/150‑1 (MQ=255)
                                                                                ccGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGc                                                                       >  1:1151269/1‑150 (MQ=255)
                                                                                     aGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATa                                                                  >  1:1244725/1‑150 (MQ=255)
                                                                                      gATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGAAGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATaa                                                                 <  1:834174/150‑1 (MQ=255)
                                                                                         tCTGACGGGAGAAAACCCTATTTTTTGCCAGATTTCTACGCATCAAATTTTGAAGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACa                                                              <  2:131705/150‑1 (MQ=255)
                                                                                            gACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTc                                                           <  2:466161/150‑1 (MQ=255)
                                                                                               gggTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGAc                                                        <  1:642991/150‑1 (MQ=255)
                                                                                                ggTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACg                                                       >  1:1472619/1‑150 (MQ=255)
                                                                                                    aaaaCCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCAtt                                                   >  2:607484/1‑150 (MQ=255)
                                                                                                     aaaCCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTg                                                  <  2:1244725/150‑1 (MQ=255)
                                                                                                      aaCCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGc                                                 >  2:696135/1‑150 (MQ=255)
                                                                                                      aaCCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGc                                                 >  2:702343/1‑150 (MQ=255)
                                                                                                       aCCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGCg                                                <  2:1071685/150‑1 (MQ=255)
                                                                                                        cccGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGCgg                                               >  1:711284/1‑150 (MQ=255)
                                                                                                         ccGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGCGGt                                              <  1:763899/150‑1 (MQ=255)
                                                                                                            aTTTTTTGCCAGATTTCGACTCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGCGGTTat                                           <  2:1189776/150‑1 (MQ=255)
                                                                                                              tttttGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGGCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGCGGTTatga                                         <  2:72514/150‑1 (MQ=255)
                                                                                                                 ttGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGCGGTTAtgatga                                      <  1:1596099/150‑1 (MQ=255)
                                                                                                                   gCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGCGGTTATGATgagg                                    <  2:510160/150‑1 (MQ=255)
                                                                                                                   gCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGCGGTTATGATgagg                                    >  2:1516850/1‑150 (MQ=255)
                                                                                                                   gCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGCGGTTATGATgagg                                    >  1:1325860/1‑150 (MQ=255)
                                                                                                                   gCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGAAGCATTGCGGTTATGATgagg                                    >  2:915696/1‑150 (MQ=255)
                                                                                                                   gCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCAGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGCGGTTATGATgagg                                    >  2:339140/1‑150 (MQ=255)
                                                                                                                    ccAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGCGGTTATGATgagga                                   <  2:1110499/150‑1 (MQ=255)
                                                                                                                      aGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGCGGTTATGATGAGGagc                                 <  2:639600/150‑1 (MQ=255)
                                                                                                                        aTTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGAAAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGCGGTTATGATGAGGagcag                               <  2:1493944/150‑1 (MQ=255)
                                                                                                                         tttCGACGCATAAAATTTTGACGGGTCATATTTTGATGCGTCAGATTTTGACGGGTCAGATTCTGAAAGTTGATAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGCGGTTATGATGAGGagcagc                              <  1:606083/150‑1 (MQ=255)
                                                                                                                           ctgACGCATCAAAATCTGACCCGTCAAAATTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGCGGTTATGATGAGGAGCAGCaa                            >  2:514738/3‑150 (MQ=38)
                                                                                                                            tgACGCATCAAAATCTGACCCGTCAAAATTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGCGGTTATGATGAGGAGCAGCaaa                           >  1:1441930/2‑150 (MQ=39)
                                                                                                                             gACGCATCAAAATCTGACCCGTCAAAATTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGTGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGAGAAACATTCGACGCAGTGCGGTTAGGATGAGGAGCAGCaaaa                          >  2:1203589/1‑150 (MQ=38)
                                                                                                                               cGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTAATAAAAATTCGACGCAATGCGGTTATGATGAGGAGCAGCAAAATg                        >  2:1702435/1‑150 (MQ=255)
                                                                                                                                gCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGCCGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCTCAACGTTACGCTGATAAACATTCGACGCATTGCGGTTATGATGAGCAGCAGCTAAATgg                       >  1:1063241/1‑150 (MQ=255)
                                                                                                                                gCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGCGGTTATGATGAGGAGCAGCAAAATgg                       <  1:417433/150‑1 (MQ=255)
                                                                                                                                 cATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGCGGTTATGATGAGGAGCAGCAAAATGGc                      >  2:1616159/1‑150 (MQ=255)
                                                                                                                                   tCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGCGGTTATGATGAGGagcag                               >  1:996716/1‑139 (MQ=255)
                                                                                                                                   tCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGAGGTTATGATGAGGAGCAGCAAAATGGCTa                    >  1:1136535/1‑150 (MQ=255)
                                                                                                                                    cAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGCGGTTATGATGAGGAGCAGCAAAATGGCTAg                   >  2:956167/1‑150 (MQ=255)
                                                                                                                                    cAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGCGGTTATGATGAGGAGCAGCAAAATGGCTAg                   <  1:16444/150‑1 (MQ=255)
                                                                                                                                             aCGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGCGGTTATGATGAGGAGCAGAAAAATGGCTAGCGAACGCAg          <  2:274644/150‑1 (MQ=255)
                                                                                                                                                ggTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGCGGTTATGATGAGGAGCAGCAAAATGGCTAGCGAACGCAGTAc       >  2:713372/1‑150 (MQ=255)
                                                                                                                                                 gTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGCGGTTATGATGAGGAGCAGCAAAATGGCTAGCGAACGCAGTACt      >  2:1694456/1‑150 (MQ=255)
                                                                                                                                                 gTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGCGGTTATGATGAGGAGCAGCAAAATGGCTAGCGAACGCAGTACt      >  1:968701/1‑150 (MQ=255)
                                                                                                                                                  tCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGCGGTTATGATGAGGAGCAGCAAAATGGCTAGCGAACGCAGTACTg     <  1:2933/150‑1 (MQ=255)
                                                                                                                                                  tCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGCGGTTATGATGAGGAGCAGCAAAATGGCTAGCGAACGCAGAACTg     <  2:933963/150‑1 (MQ=255)
                                                                                                                                                   cAGATTTTGATGCGTCAGATTTTGACGGGGCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGCGGTTATGATGAGGAGCAGCAAAATGGCTAGCGAACGCAGTACTGa    <  2:710157/150‑1 (MQ=255)
                                                                                                                                                     gATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGCGGTTATGATGAGGAGCAGCAAAATGGCTAGCGAACGCAGTACTGAtg  <  1:886764/150‑1 (MQ=255)
                                                                                                                                                     gATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGCGGTTATGATGAGGAGCAGCAAAATGGCTAGCGAACGCAGTACTGAtg  >  2:1194927/1‑150 (MQ=255)
                                                                                                                                                     gATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGCGGTTATGATGAGGAGCAGCAAAATGGCTAGCGAACGCAGTACTGAtg  >  2:1453503/1‑150 (MQ=255)
                                                                                                                                                     gATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAAAATTCGACGCATTGCGGTTATGATGAGGAGCAGCAAAATGGCTAGCGAACGCAGTACTGAtg  >  2:275193/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TCTGCGTGTCCGGTTGCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGCGGTTATGATGAGGAGCAGCAAAATGGCTAGCGAACGCAGTACTGATG  >  NC_000913/2473330‑2473628

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: