Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,473,481 T→A 6.0% pseudogene (105/708 nt) oweS ← pseudogene, CPS‑53 (KpLE1) prophage; bacteriophage replication protein O family;Phage or Prophage Related

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,473,4810TA 6.0% 290.2 / 6.7 99pseudogene (105/708 nt)oweSpseudogene, CPS‑53 (KpLE1) prophage; bacteriophage replication protein O family;Phage or Prophage Related
Reads supporting (aligned to +/- strand):  ref base T (39/54);  new base A (3/3);  total (42/57)
Fisher's exact test for biased strand distribution p-value = 6.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.25e-01

GTGTCCGGTTGCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGCGGTTATGATGAGGAGCAGCAAAATGGCTAGCGAACGCAGTACTGATGT  >  NC_000913/2473335‑2473629
                                                                                                                                                  |                                                                                                                                                    
gtgtCCGGTTGCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGAtttt                                                                                                                                                   >  1:366919/1‑150 (MQ=255)
 tgtCCGGTTGCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTg                                                                                                                                                  <  2:458406/150‑1 (MQ=255)
  gtCCGGTTGCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGa                                                                                                                                                 <  1:1100474/150‑1 (MQ=255)
   tCCGGTTGCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGAt                                                                                                                                                >  2:240960/1‑150 (MQ=255)
    ccGGTTGCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGAGGGGTCAGATTTTGATg                                                                                                                                               >  1:998031/1‑150 (MQ=255)
       gTTGCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAAATCTGACCCGTCAAAATTTGATGCGt                                                                                                                                            <  1:1696868/150‑1 (MQ=39)
        ttGCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTc                                                                                                                                           <  2:1168268/150‑1 (MQ=255)
        ttGCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAAATCTGACCCGTCAAAATTTGATGCGTc                                                                                                                                           <  1:1049346/150‑1 (MQ=39)
         tGCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCa                                                                                                                                          >  2:376673/1‑150 (MQ=255)
         tGCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCa                                                                                                                                          >  2:1156509/1‑150 (MQ=255)
          gCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCTGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAg                                                                                                                                         <  1:825123/150‑1 (MQ=255)
          gCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAg                                                                                                                                         >  2:1334321/1‑150 (MQ=255)
          gCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAg                                                                                                                                         <  1:863143/150‑1 (MQ=255)
                cGTCCGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGCATCAAAATCTGACCCGTCAAAATTTg                                                                                                                                   <  2:920163/150‑1 (MQ=255)
                 gTCCGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGa                                                                                                                                  <  2:178003/150‑1 (MQ=255)
                  tCCGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGAc                                                                                                                                 <  2:1528163/150‑1 (MQ=255)
                   ccGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACg                                                                                                                                <  2:991174/150‑1 (MQ=255)
                     ggACGAGAAGGTTTTTTATCTGACGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACggg                                                                                                                              <  1:140789/150‑1 (MQ=255)
                          agaAGGTTTTTTATCTGCCGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATCCGTCAGATTTTGACGGGTCaga                                                                                                                         >  2:134681/1‑150 (MQ=255)
                          agaAGGTTTTTTATCTGACGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCaga                                                                                                                         >  2:60092/1‑150 (MQ=255)
                          agaAGGTTTTTTATCTGACGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCaga                                                                                                                         >  2:1211292/1‑150 (MQ=255)
                              ggTTTTTTATCTGACGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCt                                                                                                                     <  2:1349846/150‑1 (MQ=255)
                              ggTTTTTTATCTGACGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTAGCCAGATTTCGACGCATCAATTTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCt                                                                                                                     <  1:711475/150‑1 (MQ=255)
                               gTTTTTTATCTGACGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTg                                                                                                                    <  1:1339445/150‑1 (MQ=255)
                                  ttttATCTGACGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACa                                                                                                                 <  2:1121026/150‑1 (MQ=255)
                                      aTCTGACGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTg                                                                                                             <  2:778461/150‑1 (MQ=255)
                                          gACGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGaa                                                                                                         <  1:46010/150‑1 (MQ=255)
                                          gACGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGaa                                                                                                         >  1:274644/1‑150 (MQ=255)
                                          gACGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGaa                                                                                                         <  1:1033043/150‑1 (MQ=255)
                                           aCGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGaaa                                                                                                        <  1:369079/150‑1 (MQ=255)
                                           aCGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGaaa                                                                                                        >  1:710157/1‑150 (MQ=255)
                                              gATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTTAGAAAATg                                                                                                     >  2:606083/1‑150 (MQ=255)
                                              gATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATg                                                                                                     <  2:1229221/150‑1 (MQ=255)
                                                tCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGcc                                                                                                   <  1:1123786/150‑1 (MQ=255)
                                                 cATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCg                                                                                                  <  1:427531/150‑1 (MQ=255)
                                                  aTGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGc                                                                                                 <  2:215643/150‑1 (MQ=255)
                                                   tgatttGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGGCAGAGTCTGACAGTTGAGAAAATGCCGCt                                                                                                <  2:232895/147‑1 (MQ=255)
                                                     ttttGATTATACTGACGTATCCCCTCCATATTCTGACGTGTGATAAACCGATTTTTTGCCAGTTTTCGACGCTTCAAATTTTGACGGGTCAGATTTTGATGCGTCAGTTTTTGACGGGTCAGAGTCTGACAGTTGAGTAAATGCCGCTGc                                                                                              <  1:1148603/150‑1 (MQ=255)
                                                      tttGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGcc                                                                                             >  2:16444/1‑150 (MQ=255)
                                                      tttGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGcc                                                                                             >  2:417433/1‑150 (MQ=255)
                                                        tGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTg                                                                                           <  1:1211292/150‑1 (MQ=255)
                                                         gATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGa                                                                                          <  2:189691/150‑1 (MQ=255)
                                                                  gACGGATCCCCGCCAGATTCTGACGGGTGAACACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCa                                                                                 >  2:263799/1‑150 (MQ=255)
                                                                    cGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAAc                                                                               >  2:886764/1‑150 (MQ=255)
                                                                    cGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAAc                                                                               >  1:693423/1‑150 (MQ=255)
                                                                     ggATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACg                                                                              <  1:1156509/150‑1 (MQ=255)
                                                                     ggATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACg                                                                              <  1:376673/150‑1 (MQ=255)
                                                                       aTCCCCGCCAGATTCAGACGGGGGAAACCCCGATTTTTTGCCAGATTTCGACCCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGCTCAGAGTCTGACAGTTGAGATAATGCCGCTGCCTGAAGCTTCGCAACGtt                                                                            <  2:1173573/150‑1 (MQ=255)
                                                                         ccccGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTaa                                                                          <  2:705111/150‑1 (MQ=255)
                                                                           ccGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGc                                                                        >  1:1151269/1‑150 (MQ=255)
                                                                           ccGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGc                                                                        <  2:1540733/150‑1 (MQ=255)
                                                                                aGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATa                                                                   >  1:1244725/1‑150 (MQ=255)
                                                                                 gATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGAAGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATaa                                                                  <  1:834174/150‑1 (MQ=255)
                                                                                    tCTGACGGGAGAAAACCCTATTTTTTGCCAGATTTCTACGCATCAAATTTTGAAGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACa                                                               <  2:131705/150‑1 (MQ=255)
                                                                                       gACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTc                                                            <  2:466161/150‑1 (MQ=255)
                                                                                          gggTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGAc                                                         <  1:642991/150‑1 (MQ=255)
                                                                                           ggTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACg                                                        >  1:1472619/1‑150 (MQ=255)
                                                                                               aaaaCCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCAtt                                                    >  2:607484/1‑150 (MQ=255)
                                                                                                aaaCCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTg                                                   <  2:1244725/150‑1 (MQ=255)
                                                                                                 aaCCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGc                                                  >  2:702343/1‑150 (MQ=255)
                                                                                                 aaCCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGc                                                  >  2:696135/1‑150 (MQ=255)
                                                                                                  aCCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGCg                                                 <  2:1071685/150‑1 (MQ=255)
                                                                                                   cccGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGCgg                                                >  1:711284/1‑150 (MQ=255)
                                                                                                    ccGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGCGGt                                               <  1:763899/150‑1 (MQ=255)
                                                                                                       aTTTTTTGCCAGATTTCGACTCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGCGGTTat                                            <  2:1189776/150‑1 (MQ=255)
                                                                                                         tttttGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGGCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGCGGTTatga                                          <  2:72514/150‑1 (MQ=255)
                                                                                                            ttGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGCGGTTAtgatga                                       <  1:1596099/150‑1 (MQ=255)
                                                                                                              gCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGCGGTTATGATgagg                                     >  2:1516850/1‑150 (MQ=255)
                                                                                                              gCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGCGGTTATGATgagg                                     <  2:510160/150‑1 (MQ=255)
                                                                                                              gCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGCGGTTATGATgagg                                     >  1:1325860/1‑150 (MQ=255)
                                                                                                              gCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGAAGCATTGCGGTTATGATgagg                                     >  2:915696/1‑150 (MQ=255)
                                                                                                              gCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCAGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGCGGTTATGATgagg                                     >  2:339140/1‑150 (MQ=255)
                                                                                                               ccAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGCGGTTATGATgagga                                    <  2:1110499/150‑1 (MQ=255)
                                                                                                                 aGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGCGGTTATGATGAGGagc                                  <  2:639600/150‑1 (MQ=255)
                                                                                                                   aTTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGAAAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGCGGTTATGATGAGGagcag                                <  2:1493944/150‑1 (MQ=255)
                                                                                                                    tttCGACGCATAAAATTTTGACGGGTCATATTTTGATGCGTCAGATTTTGACGGGTCAGATTCTGAAAGTTGATAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGCGGTTATGATGAGGagcagc                               <  1:606083/150‑1 (MQ=255)
                                                                                                                      ctgACGCATCAAAATCTGACCCGTCAAAATTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGCGGTTATGATGAGGAGCAGCaa                             >  2:514738/3‑150 (MQ=38)
                                                                                                                       tgACGCATCAAAATCTGACCCGTCAAAATTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGCGGTTATGATGAGGAGCAGCaaa                            >  1:1441930/2‑150 (MQ=39)
                                                                                                                        gACGCATCAAAATCTGACCCGTCAAAATTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGTGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGAGAAACATTCGACGCAGTGCGGTTAGGATGAGGAGCAGCaaaa                           >  2:1203589/1‑150 (MQ=38)
                                                                                                                          cGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTAATAAAAATTCGACGCAATGCGGTTATGATGAGGAGCAGCAAAATg                         >  2:1702435/1‑150 (MQ=255)
                                                                                                                           gCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGCCGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCTCAACGTTACGCTGATAAACATTCGACGCATTGCGGTTATGATGAGCAGCAGCTAAATgg                        >  1:1063241/1‑150 (MQ=255)
                                                                                                                           gCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGCGGTTATGATGAGGAGCAGCAAAATgg                        <  1:417433/150‑1 (MQ=255)
                                                                                                                            cATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGCGGTTATGATGAGGAGCAGCAAAATGGc                       >  2:1616159/1‑150 (MQ=255)
                                                                                                                              tCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGCGGTTATGATGAGGagcag                                <  2:996716/139‑1 (MQ=255)
                                                                                                                              tCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGCGGTTATGATGAGGagcag                                >  1:996716/1‑139 (MQ=255)
                                                                                                                              tCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGAGGTTATGATGAGGAGCAGCAAAATGGCTa                     >  1:1136535/1‑150 (MQ=255)
                                                                                                                               cAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGCGGTTATGATGAGGAGCAGCAAAATGGCTAg                    >  2:956167/1‑150 (MQ=255)
                                                                                                                               cAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGCGGTTATGATGAGGAGCAGCAAAATGGCTAg                    <  1:16444/150‑1 (MQ=255)
                                                                                                                                        aCGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGCGGTTATGATGAGGAGCAGAAAAATGGCTAGCGAACGCAg           <  2:274644/150‑1 (MQ=255)
                                                                                                                                           ggTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGCGGTTATGATGAGGAGCAGCAAAATGGCTAGCGAACGCAGTAc        >  2:713372/1‑150 (MQ=255)
                                                                                                                                            gTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGCGGTTATGATGAGGAGCAGCAAAATGGCTAGCGAACGCAGTACt       >  1:968701/1‑150 (MQ=255)
                                                                                                                                            gTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGCGGTTATGATGAGGAGCAGCAAAATGGCTAGCGAACGCAGTACt       >  2:1694456/1‑150 (MQ=255)
                                                                                                                                             tCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGCGGTTATGATGAGGAGCAGCAAAATGGCTAGCGAACGCAGTACTg      <  1:2933/150‑1 (MQ=255)
                                                                                                                                             tCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGCGGTTATGATGAGGAGCAGCAAAATGGCTAGCGAACGCAGAACTg      <  2:933963/150‑1 (MQ=255)
                                                                                                                                              cAGATTTTGATGCGTCAGATTTTGACGGGGCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGCGGTTATGATGAGGAGCAGCAAAATGGCTAGCGAACGCAGTACTGa     <  2:710157/150‑1 (MQ=255)
                                                                                                                                                gATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGCGGTTATGATGAGGAGCAGCAAAATGGCTAGCGAACGCAGTACTGAtg   <  1:886764/150‑1 (MQ=255)
                                                                                                                                                gATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGCGGTTATGATGAGGAGCAGCAAAATGGCTAGCGAACGCAGTACTGAtg   >  2:1194927/1‑150 (MQ=255)
                                                                                                                                                gATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGCGGTTATGATGAGGAGCAGCAAAATGGCTAGCGAACGCAGTACTGAtg   >  2:1453503/1‑150 (MQ=255)
                                                                                                                                                gATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAAAATTCGACGCATTGCGGTTATGATGAGGAGCAGCAAAATGGCTAGCGAACGCAGTACTGAtg   >  2:275193/1‑150 (MQ=255)
                                                                                                                                                 aTTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGCGGTTATGATGAGGAGCAGCAAAATGGCTAGCGAACGCAGTACTGAtgt  <  2:1151269/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
GTGTCCGGTTGCGAAGCGTCCGGACGAGAAGGTTTTTTATCTGACGGATCATGTTTTGATTTTACTGACGGATCCCCGCCAGATTCTGACGGGTGAAAACCCGATTTTTTGCCAGATTTCGACGCATCAAATTTTGACGGGTCAGATTTTGATGCGTCAGATTTTGACGGGTCAGAGTCTGACAGTTGAGAAAATGCCGCTGCCTGAAGCTTCGCAACGTTAAGCTGATAAACATTCGACGCATTGCGGTTATGATGAGGAGCAGCAAAATGGCTAGCGAACGCAGTACTGATGT  >  NC_000913/2473335‑2473629

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: