Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 3,269,956 | 0 | T | C | 24.4% | 60.8 / 19.4 | 45 | intergenic (+354/+260) | yhaC/rnpB | pentapetide repeats‑related protein/RNase P, M1 RNA component |
Reads supporting (aligned to +/- strand): ref base T (4/27); new base C (1/9); total (9/36) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.60e-01 | |||||||||||
Rejected as consensus: Frequency below/above cutoff threshold. |
GCCGCATCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAG > NC_000913/3269808‑3270104 | gccgcATCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGt < 2:103270/149‑1 (MQ=255) gccgcATCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGCAACGGTTTATTACGTACATCAGGTAAAACTGCCCGCTAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGGCATTAATCGTTCTGCCCCGCCAAAAGt > 1:112946/1‑149 (MQ=25) aTCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTaaaaa < 1:968632/149‑1 (MQ=255) tCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAAc < 2:1192649/149‑1 (MQ=255) cGGAATGTATTAATCGCACTCAAAAATCTGAAACCCGCCGGAACGGTTTTTTACGTATATGAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCATAAGCAAAAAccc < 2:85741/149‑1 (MQ=14) gCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACccgc < 2:770903/149‑1 (MQ=255) aTAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGAGTCGTGTACAGTAAATAATCATTCTGACCCACATAAAAAAATAACccgactcaac > 1:228507/1‑142 (MQ=18) cgcACTCAACAATCTGAAACCCGCCGTATCGGGTTTTTACGTAAATCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGttt < 2:677826/149‑1 (MQ=11) cACTCCACAATCATAAACCCGCCGAACCGGTTTATTACGTACAACAGGTCAAACTGACCGATAAGCCGCTTTCTTTGGGGAATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCTTCGGCGGGttttt < 1:20305/149‑1 (MQ=11) cACTCAACAATCTCAATCCCGCCGGAACGGTTTTTTACGTACACGAGGTTAAACTGACCGATAAGCCGCTTTCTTTTTGGTATAGTGTCGTGGACAGTCATTCTTCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGttttt < 2:713023/149‑1 (MQ=14) cACTCAAAAATCTTAAACCCGCCGTATCGGTTTATTACGTAAATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGttttt < 2:1269838/149‑1 (MQ=14) cACTCAAAAATATTAAACCCGCCGTAGCGGTTTTTTACGTACATCTGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCTTCGGCGGGttttt < 2:112946/149‑1 (MQ=11) aCTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTa < 2:1061508/149‑1 (MQ=255) cTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCTTCGGCGGGTTTTTAc < 2:1026858/149‑1 (MQ=255) caaTCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTaaa < 1:1129593/149‑1 (MQ=255) tCTGAAACCCGCCGGAACGGTTAATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACa < 1:177621/149‑1 (MQ=255) cTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAg < 1:460911/149‑1 (MQ=255) gAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCACATACTATAGCGTATAGTGTCGAAAAAATTAATTCATCTTTATTACACTCAAAAAGAAAAAACACTAAAAAAAATGTTTTTACGTAAAACAGGt > 2:1255491/1‑149 (MQ=255) aaaCCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTTTAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTg < 2:137342/149‑1 (MQ=255) cgGACCGGTTTTTTACGTACATCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGAc < 2:364125/149‑1 (MQ=11) cgGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGAc < 1:289478/149‑1 (MQ=255) gAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCATTCTTTTCGCTAAATTGTCGTGAACAGTCAATAATCATTATGCACAACCAAAACAAAAAAACAGAAAAAAAAAGTTTTTACGTAAAACAGGTGAAACTGACCg > 2:612628/1‑149 (MQ=255) aaCGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGa < 1:1182892/149‑1 (MQ=255) aCGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTTGGTATAGTGTCGTGGACAGTCATTAATCTTTCTGCCACTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGAt < 1:687771/149‑1 (MQ=255) gTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCgg < 2:37250/114‑1 (MQ=255) gTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCgg > 1:37250/1‑114 (MQ=255) tttATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGc < 2:979735/149‑1 (MQ=255) tattaAGTACATCAGGTTAAACTTACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCg < 1:827207/149‑1 (MQ=255) acttACATCAGGTAACACTGACCGATAAGCAGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGcttt < 1:1274354/146‑1 (MQ=255) cATCAGGTAAAACTGAGCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCTTCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCtttt < 1:1033162/149‑1 (MQ=255) cATCACTTAAAAATGTCCGATAAGCCGCTTGCATTTGGGTTTCGTGTCGGGGAAAGTCTTTCTTCTATAGGGCCCTCCAAAAGTATAAACCCGCCGAATCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCtttt < 2:629795/149‑1 (MQ=11) tcatttAATACTTAGCGATCAGATGCTTTCTTTTTTTTATTTTGTCGTGGACTGTGTTTCTTATTTCTGCCACTCAAAAAGTAAAAACCCGCCGAATCGGGTTTTTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTgg < 2:626920/144‑1 (MQ=2) acAGGTAAAAATGACCGATAAGCCGCTTTCTTTTGGGTATAGTGACGTGGACAGTCATTCATATTTCTGCCCCTCCAAAAGTTAAAACCCGCCGAATCGGGTTTTTACGTAAAACAGGTTAAACTGACCGATAAGCCGCTTTCTTTTgg < 2:964859/148‑1 (MQ=11) tgcgcctgtgtCGAGGCAAGTCCTTGATCTTAATCGCACTCAAAAAGTATAAACCCGCCGAAGCGGTTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGc < 2:1251603/142‑1 (MQ=11) tgtattttgtCGGGGACAGGCAATCTTCTTTCTGCCACTCCAAAAGTAAAAACCCGCCGAATCGGGTTATTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGcc < 1:567395/142‑1 (MQ=11) tcgtgtCGTGGAAAGTCTTTCCTCTTTCTGCCCCTCCACAAGTATAAACCCGCCGCAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTc < 2:1209849/147‑1 (MQ=11) gACAGTCATTCATCTTTCTGCCCCTCCAAAAGAAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGAc > 2:835249/1‑79 (MQ=21) tcattcatCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGCACCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAAccc < 2:1235723/149‑1 (MQ=255) tcattcatCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGAc > 2:960951/1‑74 (MQ=255) tGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGaa > 2:441807/1‑142 (MQ=37) tGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGaa < 1:441807/142‑1 (MQ=37) cacTCCAAAATTATAAACCCGCCGTATGGGGTTTTTACGTAAAACAGGTTAAACTGACCGATAAGCGGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCTTCGGCGGGttttt < 2:1158892/147‑1 (MQ=11) cTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGtcattca < 2:842551/102‑1 (MQ=40) cTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGtcattca > 1:842551/1‑102 (MQ=40) cTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCAGCTTTCTTTTGGGTATAGTGTCGTGTACAGTCATTCAACTTTATGAACCTAAAAAAGCAAAAACCAGCCTAATCGGGTTTTTaa > 2:720972/1‑148 (MQ=11) cTCCAAAAGCAAAACCCCGCCGTCTCGGGTTTTTACGTACAACAGCTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGGCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTAc < 2:852649/149‑1 (MQ=11) aGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGAAAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAcc < 1:1294950/149‑1 (MQ=38) tAAAAACCCGCCGCACCGTGTTTTTACGTAAAACAGGTGAAACTGTCCGATAAGCCGCTTTCTTTTGGGTTTAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCTAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAg < 1:951434/149‑1 (MQ=11) | GCCGCATCCGGCATGAATAAAGCGCACTCAACAATCTGAAACCCGCCGGAACGGTTTATTACGTACATCAGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAG > NC_000913/3269808‑3270104 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |