Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,019,460 G→A V359I (GTC→ATC)  fliK → flagellar hook‑length control protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,019,4600GA98.7% 241.1 / ‑6.1 76V359I (GTC→ATC) fliKflagellar hook‑length control protein
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (35/40);  total (36/40)
Fisher's exact test for biased strand distribution p-value = 4.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAAGTGGCATTCAGTTAGGGCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGACACAGGA  >  NC_000913/2019314‑2019605
                                                                                                                                                  |                                                                                                                                                 
aaaGTGGCATTCAGTTAGGGCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATCCGCTTCCGGTTCCCAtc                                                                                                                                                 >  1:835689/1‑149 (MQ=255)
 aaGTGGCATTCAGTTAGGGCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGGACAGCAAACCATGAACCTCTGGGGGGGGAAGACGACGATACGCTTCCGGTTCCCAtct                                                                                                                                                <  1:1142135/149‑1 (MQ=255)
   ggggCATTCAGTTAGGGCAAAGCAACATCAGGGGCGAAAGCGTTAGTGGGCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACAATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATctct                                                                                                                                              <  1:967291/147‑1 (MQ=255)
      gCATTCAGTTAGGGCAAAGCAACATTAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTa                                                                                                                                           <  1:311110/149‑1 (MQ=255)
      gCATTCAGTTAGGGCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTa                                                                                                                                           >  2:246063/1‑149 (MQ=255)
      gCATTCAGTTAGGGCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTa                                                                                                                                           >  1:679308/1‑149 (MQ=255)
         ttCAGTTAGGGCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACaa                                                                                                                                        <  2:800247/149‑1 (MQ=255)
         ttCAGTTAGGGCAAAGCAACATCAGGGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACaa                                                                                                                                        <  2:885036/149‑1 (MQ=255)
          tCAGTTAGGGAAAAGCAACATAAGTGGCGAAAGCTTTAGTGGTCAGCTGCTGGCCGCTTACCAGCAACATCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAg                                                                                                                                       <  2:622741/149‑1 (MQ=255)
            aGTTAGGGCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAggg                                                                                                                                     >  2:444103/1‑149 (MQ=255)
             gTTAGGGCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGc                                                                                                                                    <  2:405112/149‑1 (MQ=255)
               tAGGGCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAACCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCgt                                                                                                                                  <  2:930582/149‑1 (MQ=255)
               tAGGGCAAAGCAACATCAGTCGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCgt                                                                                                                                  <  2:337615/149‑1 (MQ=255)
                tgggCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGGTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCGCATCTCTTTACAAGGGCgtg                                                                                                                                 <  2:529174/148‑1 (MQ=255)
                aGGGCTACGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACAATGAACCTCTGTCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCgtg                                                                                                                                 <  2:872707/149‑1 (MQ=255)
                aGGGCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCgtg                                                                                                                                 >  2:1071413/1‑149 (MQ=255)
                  ggCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTa                                                                                                                               >  2:122809/1‑149 (MQ=255)
                   gaaaaGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCAAGCAACAGCAAAGCCAACGCACAGCAAACATTGAACCTCTGGCGGGTGAAGCCGACGATACGGTTCCGGTTCCCATGTATTTACAAGGTCgtaaaa                                                                                                                              >  2:1253805/3‑145 (MQ=255)
                    cAAACCAACATAAGTGGCGAAAGCTTTAGAGGTCAGCAGCAGGCCGCTTCCCAGCAAAAGCAAAGCCAACGCACAGCAAACCATGAACCACTTGCGGGTGATGACGACTAAACGCTTCCGCTTCCCATCTCATTACAAGGGAGTGTAAc                                                                                                                             >  2:325357/1‑149 (MQ=255)
                      aaGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAg                                                                                                                           <  1:294949/149‑1 (MQ=255)
                            cATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTTACAGGCAACa                                                                                                                     <  1:246063/149‑1 (MQ=255)
                            cATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACa                                                                                                                     >  2:1269621/1‑149 (MQ=255)
                                ttgggCGAACGCTTTAGTGGTCAGCAGCCGGCCGCTACCCAGCAACAGCAAAGCCACCGCACACCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACACGGGCGTGTAACAGGCAACAgcgg                                                                                                                 <  2:924321/146‑1 (MQ=255)
                                  tGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAACGCCAACGCCCAGCAAACCAGGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCGGCTCTTTTCAAGGGCGTGTAACAGGCACCAgcggcg                                                                                                               >  2:633396/1‑149 (MQ=255)
                                    gCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGTGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGtt                                                                                                             <  2:907057/149‑1 (MQ=255)
                                       aaaGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGat                                                                                                          <  2:1264882/149‑1 (MQ=255)
                                       aaaGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGat                                                                                                          <  1:207114/149‑1 (MQ=255)
                                        aaGCTATAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGATTCCGGTTCCCAGCTCTTTACCAGGGCGTGTAACAGGCAACAGCGGCGTTGata                                                                                                         >  1:248866/1‑149 (MQ=255)
                                            tttAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATAtttt                                                                                                     >  1:6410/1‑149 (MQ=255)
                                               aGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGc                                                                                                  <  2:910091/149‑1 (MQ=255)
                                                 tGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCt                                                                                                >  2:253857/1‑149 (MQ=255)
                                                   gTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGCCGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTaa                                                                                              >  1:1134723/1‑149 (MQ=255)
                                                   gTCAGCAGCAGGACGCTTCGCAGCAACAGCAAAGCAAACGCACAGCTAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTTGCCTaa                                                                                              <  1:1088970/149‑1 (MQ=255)
                                                        cagcagGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCa                                                                                         >  2:727825/1‑149 (MQ=255)
                                                         agcagGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAACCCATGAACCTCTGGCGGGGGACGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCag                                                                                        <  1:1273968/149‑1 (MQ=255)
                                                         agcagGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCag                                                                                        <  1:403754/149‑1 (MQ=255)
                                                          gcagGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCaga                                                                                       <  1:1005841/149‑1 (MQ=255)
                                                          gcagGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCaga                                                                                       <  1:758130/149‑1 (MQ=255)
                                                           ccggCCGCTTCCCTGCAACATCAAAGCCAACGCAAAGCAAACCATGAACATCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCagag                                                                                      <  1:1175240/147‑1 (MQ=255)
                                                           cagGCCGCTTCCCACCAACAGCAAAGCCAACGCTCAGCAAACCATCAACCTCTGGCGGGTGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCagag                                                                                      <  1:1170099/149‑1 (MQ=255)
                                                            tggCCGCTTCCCAGCAACAGCAATGCCAACGCACAGCAATCCATGAACCTCTGGGGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAgg                                                                                     <  2:112977/148‑1 (MQ=255)
                                                                   ttCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCAcc                                                                              <  2:423398/149‑1 (MQ=255)
                                                                      ccAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTa                                                                           >  1:483633/1‑149 (MQ=255)
                                                                        agcaacagcaaAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAAt                                                                         >  2:755458/1‑149 (MQ=255)
                                                                         gcaacagcaaAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATc                                                                        >  2:1110928/1‑149 (MQ=255)
                                                                          caacagcaaAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATcc                                                                       >  1:240849/1‑149 (MQ=255)
                                                                            acagcaaAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCcgc                                                                     <  2:598503/149‑1 (MQ=255)
                                                                              agcaaAGCCAACGCACAGCAAACCATGAAACTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGt                                                                   <  2:196883/149‑1 (MQ=255)
                                                                               gcaaAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAAACCGCGTc                                                                  >  2:104449/1‑149 (MQ=255)
                                                                                caaAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCt                                                                 >  1:521518/1‑149 (MQ=255)
                                                                                caaAGCAAAAGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCTGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGTTAGCACCGTAATCCGCGTCt                                                                 >  2:893249/1‑149 (MQ=255)
                                                                                 aaaGCAATCGCACAGCACACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCtt                                                                <  1:196554/149‑1 (MQ=255)
                                                                                  aaGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCttt                                                               <  2:49215/149‑1 (MQ=255)
                                                                                    gCCAACGCACAGCAAACCATGATCCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTACCATCTCTTTAAAAGGGCGTGTAACAGGCAACAGCGGCGTTGTTATTTTCGCCTTACGTCAGATGTAGCAGCGTAATCCGCGTCTATTc                                                             >  2:513056/1‑149 (MQ=255)
                                                                                    gCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTc                                                             <  1:540226/149‑1 (MQ=255)
                                                                                        aCGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAGCGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCg                                                         >  2:1274697/1‑149 (MQ=255)
                                                                                         cGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGc                                                        <  1:122809/149‑1 (MQ=255)
                                                                                          gCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCt                                                       >  2:1240120/1‑149 (MQ=255)
                                                                                              aGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGGCTTTTCCCCGCAttgt                                                   >  2:560118/1‑149 (MQ=255)
                                                                                                 aaaCCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTgc                                                >  1:1017563/1‑149 (MQ=255)
                                                                                                 aaaCCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCGttgttgg                                                >  1:1269317/1‑148 (MQ=255)
                                                                                                               tGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAgg                                  >  2:866765/1‑149 (MQ=255)
                                                                                                               tGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAgg                                  >  1:52224/1‑149 (MQ=255)
                                                                                                               tGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAgg                                  >  1:1221605/1‑149 (MQ=255)
                                                                                                                    ggggAAGACTACGATACGCTTCAGGTTCCCATCTCTTTACAAGCGCGCGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAAt                             <  2:191977/149‑1 (MQ=255)
                                                                                                                      ggAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATAATTTCGCCTAACGTCAGAGGTAGCACCGTAATCCTCGACTTTTCACCGCTTTGTTGCGCTCAAGACGCAGGATAATTa                           >  1:263335/1‑149 (MQ=255)
                                                                                                                       gAAGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAg                          <  2:679308/149‑1 (MQ=255)
                                                                                                                        aaGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGc                         <  1:719696/149‑1 (MQ=255)
                                                                                                                         aGACGACGATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTACCGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGcc                        <  2:114720/149‑1 (MQ=255)
                                                                                                                           acgacgATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGa                      >  2:452805/1‑149 (MQ=255)
                                                                                                                           acgacgATACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGa                      <  1:250726/149‑1 (MQ=255)
                                                                                                                             gacgaTACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATa                    >  2:97612/1‑149 (MQ=255)
                                                                                                                                  tACGCTTCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAg               >  1:508801/1‑149 (MQ=255)
                                                                                                                                        tCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGa         <  2:483633/149‑1 (MQ=255)
                                                                                                                                        tCCGGTTCCCATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGa         <  2:706429/149‑1 (MQ=255)
                                                                                                                                               cccATCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGACACAGGa  >  1:572651/1‑149 (MQ=255)
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AAAGTGGCATTCAGTTAGGGCAAAGCAACATCAGTGGCGAAAGCTTTAGTGGTCAGCAGCAGGCCGCTTCCCAGCAACAGCAAAGCCAACGCACAGCAAACCATGAACCTCTGGCGGGGGAAGACGACGATACGCTTCCGGTTCCCGTCTCTTTACAAGGGCGTGTAACAGGCAACAGCGGCGTTGATATTTTCGCCTAACGTCAGAGGTAGCACCGTAATCCGCGTCTTTTCCCCGCTTTGTTGCGCTCAAGACGCAGGATAATTAGCCGATAAGCAGTAGCGACACAGGA  >  NC_000913/2019314‑2019605

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑