Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,815,809 Δ1 bp 45.4% intergenic (‑41/+25) pyrE ← / ← rph orotate phosphoribosyltransferase/ribonuclease PH (defective);enzyme; Degradation of RNA; RNase PH

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,815,8090G.45.4% 394.7 / inf 520intergenic (‑41/+25)pyrE/rphorotate phosphoribosyltransferase/ribonuclease PH (defective);enzyme; Degradation of RNA; RNase PH
Reads supporting (aligned to +/- strand):  ref base G (87/106);  new base . (181/55);  total (278/242)
Fisher's exact test for biased strand distribution p-value = 2.25e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 564 total aligned reads displayed.

CGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAAT  >  NC_000913/3815660‑3815958
                                                                                                                                                     |                                                                                                                                                     
cGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAg                                                                                                                                                       >  1:9649242/1‑150 (MQ=255)
     aagaaATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGCGACt                                                                                                                                                 >  2:10086656/1‑150 (MQ=255)
       gaaATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGCGACtca                                                                                                                                               >  2:1318188/1‑150 (MQ=255)
       gaaATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTAATTTTCTACAGACAAAAAAAAGCGACtca                                                                                                                                               >  2:106709/1‑150 (MQ=255)
        aaaTCGGGGGTTTTGCGCCCGGATTTCAGCTTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGAAAATTCAATAAAGGGGCGCTGATTTGGTTTAATGCCTTCGCTCCTCATCTTACTTTTCTACAGAAAAAAAAAAGCGACtcat                                                                                                                                              <  2:7177314/150‑1 (MQ=255)
             ggggCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGCGACTCATCAGTc                                                                                                                                         >  2:9801855/1‑150 (MQ=255)
              gggCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGCGACTCATCAGTCg                                                                                                                                        >  1:9951751/1‑150 (MQ=255)
              gggCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGCGACTCATCAGTCg                                                                                                                                        >  1:6870336/1‑150 (MQ=255)
              gggCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGCGACTCATCAGTCg                                                                                                                                        >  1:4531575/1‑150 (MQ=255)
                  ttttGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGCGACTCATCAGTCGCCtt                                                                                                                                    >  1:2567579/1‑150 (MQ=255)
                   tttGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCtt                                                                                                                                    >  2:2358703/1‑150 (MQ=255)
                    ttGCGCCTTTATTTAAGCGTAAACTCGCAAAACTTTAACACCTGTTTTATAAGCGAAAATCCAATAAACGGGCGCTTATATGGTTACATGCCTGCGCTCCTAATCTTAATTTTCAACCGACAAAAAAAAGCGACTCATCAGTCGCCTTaa                                                                                                                                  <  2:8636128/150‑1 (MQ=255)
                      gcgcCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCACCTTaaa                                                                                                                                 >  1:2179160/1‑150 (MQ=255)
                        gcCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAAt                                                                                                                              >  1:7353663/1‑150 (MQ=255)
                         cccGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAAt                                                                                                                              >  2:7894248/1‑150 (MQ=255)
                         cccGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCACATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAAt                                                                                                                              <  2:4705492/150‑1 (MQ=255)
                          ccGGATTTAAGCTTAAACTCCCCAAAATTAAACACCGGCTTCCTTAGCGCAAATTCAAAACCCGGGCGCTGATATGGTTACATGCTTTCGCTGCAAATCTTATTTTTCTACACAAAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCa                                                                                                                            <  2:9562651/150‑1 (MQ=255)
                              aTTTCCGCTTAAACTCGCAAAACTTTAACCCCTGCTTGCTAAGCGCAAATTCCATAACCTGGCGCTTATATGGTTTCCTGCCTTCGCCCCTCATATTACTTTTACACCCAAAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGttt                                                                                                                        <  2:4345076/150‑1 (MQ=255)
                                 tCAGGGAAAACCCGCCAAACTTTAACACCTGATTGATATGCGAAAATTCAATAACCTGGCGCTTATTTGGTTACATCCCTTCGCTCCCCATCTTACTTTTATACACACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGcc                                                                                                                     <  2:3505067/150‑1 (MQ=255)
                                     cGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAgc                                                                                                                  >  1:8835644/1‑150 (MQ=255)
                                          aCTCGCCAAACTTTACCACCTGCTTGCTATGCGAAATTTCAATAACCTGGCGCTTTTTTGTTTTCATGCCTTGGCTCCACATATTACTTTTCTACAGAAAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCC                                                                                                            <  2:8925541‑M1/150‑1 (MQ=255)
                                            tCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTT                                                                                                          >  1:6394696‑M1/1‑150 (MQ=255)
                                              gCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCT                                                                                                        >  1:3006077‑M1/1‑150 (MQ=255)
                                              ccaaaaCTTTCACACCTGCTTGCTACGCTCAAATTCAAAAACGGGGCGGTTATTTGTTTTCTGGCCTTCGCTCCTAACCTATCTTTCCTCCCCAAAAAAAAACGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCt                                                                                                        <  2:10393731/147‑1 (MQ=255)
                                               ccAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTG                                                                                                       >  1:4179689‑M1/1‑150 (MQ=255)
                                                  aaCTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGt                                                                                                    >  2:2572326/1‑150 (MQ=255)
                                                     tttAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGc                                                                                                 >  1:3639391/1‑150 (MQ=255)
                                                       tAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCt                                                                                                >  1:5788963/1‑150 (MQ=255)
                                                         acacCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTAc                                                                                              >  1:5166608/1‑150 (MQ=255)
                                                         acacCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTAc                                                                                              >  2:4464174/1‑150 (MQ=255)
                                                         acacCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACa                                                                                             >  2:10178394/1‑150 (MQ=255)
                                                           cccggcttgctAAGCGCAAATCCAATAACCGGGCGCTTATCGGGTTACATGCCTTCGCACCTCCACTTACTTTACTACAGACAAAAAAACGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAAt                                                                                           <  2:6708951/146‑1 (MQ=255)
                                                           acCTGATTGCTAAGCGCAATTTCAATAAACTGGCGCTTATATGGTTTCTTGCCTTCGCGCCTCATCTTACTTTTCTACAGACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAAt                                                                                           <  2:747835/150‑1 (MQ=255)
                                                            ccTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATg                                                                                          <  1:501205/150‑1 (MQ=255)
                                                             cTGCTTGCTAAGCGCAAATTCAATAACCTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATg                                                                                          <  2:9292658/150‑1 (MQ=255)
                                                              tgcttgctAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGga                                                                                        >  1:1267367/1‑150 (MQ=255)
                                                                ctggctACGCGAAAATTCAATAAACTGGCGCTTATATGGTTTCATGCCTCCGACCCTCTCCTCACTTTTCTACCCACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGgatt                                                                                      <  2:9637246/147‑1 (MQ=255)
                                                                   gctAAGCGAAAATACAATAACCTGGCGCTGATATGGTTTCATGCCTCCGCGCCTAATCTTACTTTTCTACAGACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGgattcga                                                                                   <  2:2592665/150‑1 (MQ=255)
                                                                     tAAGCGCAAATTCAATAAACTGGCGCTGATAAGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAAAGgattcgatt                                                                                 >  1:4852713/1‑150 (MQ=255)
                                                                       aGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGgattcgattc                                                                                <  1:6023259/150‑1 (MQ=255)
                                                                                   aTAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcg                                                                    <  1:343056‑M1/150‑35 (MQ=255)
                                                                                     acaCTGGCGGTTTTTTGGTTCCAGGCGTTCGCTCCTCAACTTACTTTTCTACACACAAAAAACCGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGc                                                                 <  1:4419650/148‑1 (MQ=255)
                                                                                     aaccTGGGGCTGTTATGGTTCCTGGCGTGCGCTCATAATCTACCTTTACAACCGACAAAAAAACGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGc                                                                 <  2:8396863/147‑1 (MQ=255)
                                                                                         tGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCcaa                                                              <  2:7636863/150‑1 (MQ=255)
                                                                                            cgcTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCcaacaa                                                           <  2:4656534/150‑1 (MQ=255)
                                                                                             gcTGATTTGGTTTCTTGCCTCCGCGCATCTTTTTATTTTTCTACACACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGa                                                         <  1:1668541/150‑1 (MQ=255)
                                                                                               tGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGAt                                                        <  2:2187812/150‑1 (MQ=255)
                                                                                                 atatGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcatcactacgtt                                                      >  1:10201109‑M1/1‑102 (MQ=255)
                                                                                                 atatGGTTACATGCCTTCGCTCCACATCTAACTTTTCTACACACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAg                                                     <  2:6319113/150‑1 (MQ=255)
                                                                                                   atGTTTTCTTCCCTGCGCCCCTAACATAACTTTCCTACAAAAAAAAAAACGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTa                                                   <  2:3027146/150‑1 (MQ=255)
                                                                                                     ggTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcatcactacgttcatg                                                  >  1:7003822‑M1/1‑98 (MQ=255)
                                                                                                     ggTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGc                                                 >  1:5615384/1‑150 (MQ=255)
                                                                                                      gTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcatcactacgttcatgt                                                 >  2:10752203‑M1/1‑97 (MQ=255)
                                                                                                           aTGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGcc                                                                <  2:9101462/129‑1 (MQ=255)
                                                                                                           aTGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGcc                                                                >  1:9101462/1‑129 (MQ=255)
                                                                                                            tGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCAt                                          <  1:9822587/150‑1 (MQ=255)
                                                                                                            tGCCTTCACTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCa                                           <  2:7274977/150‑1 (MQ=255)
                                                                                                             gCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCCACACATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCGTCTGCGTCGCTACAATGGATTCGATTCCCCGCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCAt                                          >  1:8267243/1‑150 (MQ=255)
                                                                                                              ccTTCGCTCCTCATCTTACTTTTCTACAGAAAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATgg                                        <  2:3409178/150‑1 (MQ=255)
                                                                                                              ccTGCGATCCTAATCTTACTTTTCTACACAAAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATgg                                        <  2:400572/150‑1 (MQ=255)
                                                                                                               cTTCGCTCCTCCTCTTACTTTTCTACAGACACAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATgg                                        <  2:5796692/150‑1 (MQ=255)
                                                                                                                ttCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAATCGACTCATAAGTCGCATTAAAAATCAGTTTTCCATCGACTCATTATGCGTCGCTACAATGGATAAGAATCCCCTCGGGCCAGAGACAACAAGATGAGTAGATCGTAAAGGGt                                      >  2:3139506/1‑150 (MQ=255)
                                                                                                                 tCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGt                                      <  2:7047833/150‑1 (MQ=255)
                                                                                                                 tCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCACCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGt                                      <  1:10695939/150‑1 (MQ=255)
                                                                                                                   gccccacATCTTACTTTACTACACAAAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGaa                                   <  2:2935652/144‑1 (MQ=255)
                                                                                                                    ctcctcATTTTACTTTTATAAAAAAAAAAAAACGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAAc                                  <  2:2802151/150‑1 (MQ=255)
                                                                                                                      cctcATCTTACTTTTCTACATACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACgg                                <  2:3712615/150‑1 (MQ=255)
                                                                                                                       ctcATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACgg                                >  1:3247041/1‑150 (MQ=255)
                                                                                                                          aTCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTc                             <  2:1546035/150‑1 (MQ=255)
                                                                                                                            cTTACTTTTCTACATACAAAAAAAAGGCGACTCATCAGTCACCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGc                           <  2:5941801/150‑1 (MQ=255)
                                                                                                                            cTTACTTTTCTACAGACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGcc                          >  1:5365543/1‑150 (MQ=255)
                                                                                                                              tACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCt                         <  1:7919152/150‑1 (MQ=255)
                                                                                                                                attttCTACATAAAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCt                      <  1:10500137/149‑1 (MQ=255)
                                                                                                                                 ttttCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCt                      >  2:1742110/1‑150 (MQ=255)
                                                                                                                                 ttttCTACAGACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTg                     >  1:2678012/1‑150 (MQ=255)
                                                                                                                                 ttttCTACAGACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTg                     >  2:9703695/1‑150 (MQ=255)
                                                                                                                                 ttttCTACAGACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTg                     >  2:10909657/1‑150 (MQ=255)
                                                                                                                                 ttttCTACAGACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTg                     >  2:4200618/1‑150 (MQ=255)
                                                                                                                                   ttCTACAGACAAAAAAAAGGCGACTCATCAGTCACCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGc                    >  1:3790365/1‑150 (MQ=255)
                                                                                                                                     cTACAGACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGt                 <  2:7332044/150‑1 (MQ=255)
                                                                                                                                      tacatacaAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTc                >  1:782193/1‑150 (MQ=255)
                                                                                                                                      tACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgat                                                                                 <  2:6457460‑M1/86‑22 (MQ=255)
                                                                                                                                      tACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGt                 >  1:1943370/1‑150 (MQ=255)
                                                                                                                                       aCAGACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTcc               >  1:1414644/1‑150 (MQ=255)
                                                                                                                                         cacaaaaaaaaaAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTccc              <  1:10760170/146‑1 (MQ=255)
                                                                                                                                         aGACAAAAAAAAGGCGACTCATCAGACGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCcgtcccctgcacttcaatgatgcgcccgtcttcggtcatcactacgttcatgtcggtctctgcggcagagtcttcaacgtattacaga              >  1:9538039‑M1/1‑62 (MQ=255)
                                                                                                                                          gACAAAAAAAAGTGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCt            >  1:2329578/1‑150 (MQ=255)
                                                                                                                                          gACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCt            <  1:6281964/150‑1 (MQ=255)
                                                                                                                                          gACAAAAAAAAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGC                                                                                                             >  1:7184263‑M1/1‑53 (MQ=255)
                                                                                                                                            acaaaaaaaaGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTg           >  1:6653580/3‑150 (MQ=255)
                                                                                                                                            acaaaaaaaaGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTg           >  1:7694235/3‑150 (MQ=255)
                                                                                                                                            acaaaaaaaaGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTg           >  2:5688344/3‑150 (MQ=255)
                                                                                                                                            acaaaaaaaaGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTg           >  2:5501875/3‑150 (MQ=255)
                                                                                                                                              aaaaaaaaGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCa         >  2:5090544/1‑150 (MQ=255)
                                                                                                                                              aaaaaaaaGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCa         >  2:1968393/1‑150 (MQ=255)
                                                                                                                                              aaaaaaaaGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCa         >  2:6106703/1‑150 (MQ=255)
                                                                                                                                               aaaaaaAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCAc        >  1:1473496/1‑150 (MQ=255)
                                                                                                                                                 aaaaGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACtt      >  1:8113191/1‑150 (MQ=255)
                                                                                                                                                 aaaaAGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACtt      <  2:4153772/150‑1 (MQ=255)
                                                                                                                                                     ggCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAat  >  2:5322757/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CGTTGAAGAAATAGGGGCTTTTGCGCCCGGATTTCAGCGTAAACTCGCCAAACTTTAACACCTGCTTGCTAAGCGCAAATTCAATAAACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCGATTCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAAT  >  NC_000913/3815660‑3815958

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: