Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,708,429 T→C 6.2% intergenic (‑724/+187) dppA ← / ← proK dipeptide/heme ABC transporter periplasmic binding protein; dipeptide chemotaxis receptor/tRNA‑Pro

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,708,4290TC 6.2% 1903.7 / 74.1 592intergenic (‑724/+187)dppA/proKdipeptide/heme ABC transporter periplasmic binding protein; dipeptide chemotaxis receptor/tRNA‑Pro
Reads supporting (aligned to +/- strand):  ref base T (246/308);  new base C (15/22);  total (261/331)
Fisher's exact test for biased strand distribution p-value = 7.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.21e-01

Only 100 of 616 total aligned reads displayed.

AATAAGGCGGTCGCGCCGCATCCGACAATGGCCGGCACATTTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAT  >  NC_000913/3708280‑3708578
                                                                                                                                                     |                                                                                                                                                     
aaTAAGGCGGTCGCGCCGCATCCGACAATGGCCGGCACATTTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAATAGCCTGCTCGtt                                                                                                                                                       >  1:6267850/1‑150 (MQ=255)
aaTAAGGCGGTCGCGCCGCATCCGACAATGGCCGGCACATTTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGtt                                                                                                                                                       <  1:956168/150‑1 (MQ=255)
aaTAAGGCGGTCGCGCCGCATCCGACAATGGCCGGCACATTTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGtt                                                                                                                                                       <  1:7028410/150‑1 (MQ=255)
 aTAAGGCGGTCGCGCCGCATCCGACAATGGCCGGCACATTTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAATAGCCTGCTCGTTg                                                                                                                                                      >  1:10359682/1‑150 (MQ=255)
  tAAGGCGGTCGCGCCGCATCCGACAATGGCCGGCACATTTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGa                                                                                                                                                     <  1:4263998/150‑1 (MQ=255)
       cGGTCGCGCCGCATCCGACAATGGCCGGCACATTTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAgg                                                                                                                                                <  1:5594205/150‑1 (MQ=255)
        ggTCGCGCCGCATCCGACAATGGCCGGCACATTTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGc                                                                                                                                               <  1:2900575/150‑1 (MQ=255)
        ggTCGCGCCGCATCCGACAATGGCCGGCACATTTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCAACGAGCAGGc                                                                                                                                               <  2:1820369/150‑1 (MQ=255)
        ggTCGCGCCGCATCCGACAATGGCCGGCACATTTGCCTGATGCGACGCTGACGCGTCTTATCAGACCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGc                                                                                                                                               <  1:10434534/150‑1 (MQ=255)
         gTCGCGCCGCATCCGACAATGGCCGGCACATTTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCAACGAGCAGGCt                                                                                                                                              <  2:6449287/150‑1 (MQ=255)
          tCGCGCCGCATCCGACAATGGCCGGCACATTTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCAACGAGCAGGCtt                                                                                                                                             <  2:5123058/150‑1 (MQ=255)
          tCGCGCCGCATCCGACAATGGCCGGCACATGTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCtt                                                                                                                                             <  1:6709094/150‑1 (MQ=255)
            gcgcCGCATCCGACCATGGCCGGCACATGTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCAACGAGCAGGCtttt                                                                                                                                           <  2:2076650/150‑1 (MQ=25)
               ccgcATCCGACAATGGCCGGCACATTTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGa                                                                                                                                        >  2:8311445/1‑150 (MQ=255)
               ccgcATCCGACAATGGCCGGCACATTTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTGTTCgc                                                                                                                                        >  2:4248531/1‑149 (MQ=255)
                  cATCCGACAATGGCCGGCACATTTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTGTTCGAAtt                                                                                                                                     >  1:5039941/1‑150 (MQ=255)
                    tCCGACAATGGCCGGCACATTTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTg                                                                                                                                   <  2:7441620/150‑1 (MQ=255)
                    tCCGACAATGGCCGGCACATTTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTg                                                                                                                                   <  1:10034751/150‑1 (MQ=255)
                       gACAATGGCCGGCACATTTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGATCCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTc                                                                                                                                <  2:969828/150‑1 (MQ=255)
                       gACAATGGCCGGCACATTTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCTAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTc                                                                                                                                <  2:6775775/150‑1 (MQ=255)
                       gACAATGGCCGGCACATTTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTc                                                                                                                                >  1:37911/1‑150 (MQ=255)
                            tGGCCGGCACATTTGCCTGATGCGACTCTGACGCGTCTTATCAGTCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCACATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGa                                                                                                                           <  2:693313/150‑1 (MQ=255)
                            tGGCCGGCACATTTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGa                                                                                                                           >  2:6354520/1‑150 (MQ=255)
                             ggCCGGCACATTTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCGGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGAt                                                                                                                          <  2:3600336/150‑1 (MQ=255)
                              gCCGGCACATTTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATa                                                                                                                         >  1:1179812/1‑150 (MQ=255)
                                cGGCACATTTGCCTGATGCGACGCTGACGCGTCGTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGACTTTGGTCGGTGATaga                                                                                                                       <  2:10656889/150‑1 (MQ=255)
                                 ggCACATTTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATagag                                                                                                                      <  1:597687/150‑1 (MQ=255)
                                 ggCACATTTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATagag                                                                                                                      <  2:2500365/150‑1 (MQ=255)
                                   cacaTTTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGa                                                                                                                    <  2:5454878/150‑1 (MQ=255)
                                   cacaTTTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGa                                                                                                                    >  1:1494903/1‑150 (MQ=255)
                                      aTTTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGTTCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTa                                                                                                                 <  1:7468209/150‑1 (MQ=255)
                                      aTTTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTc                                                                                                                                          >  1:8428085/1‑125 (MQ=255)
                                      aTTTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGAttc                                                                                                                 >  2:3269644/1‑149 (MQ=255)
                                       tttGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTAc                                                                                                                >  2:7988101/1‑150 (MQ=255)
                                          gCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCg                                                                                                             <  2:2375470/150‑1 (MQ=255)
                                             tGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGcc                                                                                                           >  2:900817/1‑149 (MQ=255)
                                             tGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGcc                                                                                                           <  1:900817/149‑1 (MQ=255)
                                                   gACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGt                                                                                                    >  1:10618309/1‑150 (MQ=255)
                                                       cTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGtt                                                                                                                                                       >  1:5465675/1‑95 (MQ=255)
                                                       cTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCt                                                                                                <  1:7850582/150‑1 (MQ=255)
                                                         gACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCg                                                                                              <  1:920085/150‑1 (MQ=255)
                                                           cgcgTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGcc                                                                                            <  2:1211142/150‑1 (MQ=255)
                                                                cTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCg                                                                                       <  1:533016/150‑1 (MQ=255)
                                                                 ttATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCgg                                                                                      <  2:8558707/150‑1 (MQ=255)
                                                                 ttATCAGGCCGACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCgg                                                                                      <  1:6507548/150‑1 (MQ=255)
                                                                   aTCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGc                                                                                    <  2:9462610/150‑1 (MQ=255)
                                                                   aTCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGc                                                                                    <  2:1051620/150‑1 (MQ=255)
                                                                         ccTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTACCACCGTCGAGGAGCGCCCTGAGGGCCGTTGc                                                                              >  2:201074/1‑150 (MQ=14)
                                                                          cTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCt                                                                             <  1:2118871/150‑1 (MQ=255)
                                                                           tACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTg                                                                            <  2:5548381/150‑1 (MQ=255)
                                                                            aCGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTgg                                                                           <  1:729223/150‑1 (MQ=255)
                                                                             cGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGgc                                                                          <  1:1657013/150‑1 (MQ=255)
                                                                              gtcgagtcgaACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGgcg                                                                         <  1:8225800/150‑1 (MQ=255)
                                                                               tcgagtcgaACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGgcgc                                                                        >  2:10259451/1‑150 (MQ=255)
                                                                                  agtcgaACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAAc                                                                     <  1:8807448/150‑1 (MQ=255)
                                                                                  agtcgaACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAAc                                                                     <  1:10214967/150‑1 (MQ=255)
                                                                                    tcgaACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAAc                                                                     <  1:5806651/148‑1 (MQ=255)
                                                                                         ccTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTtctct                                                              >  1:4560422/1‑150 (MQ=255)
                                                                                          cTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTtctctc                                                             >  1:3223284/1‑150 (MQ=255)
                                                                                           tCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCtcg                                                            >  2:3128700/1‑150 (MQ=255)
                                                                                             ccTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCtcgct                                                          >  2:4457484/1‑150 (MQ=255)
                                                                                               tGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAATTCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGGGGATCGCCCTGCGGGCCGTTGATGTCGCAACGTtgtctgggttg                                                        >  2:3481021/1‑144 (MQ=18)
                                                                                                 cccccGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCtcgcttcgc                                                      >  2:1391838/1‑150 (MQ=255)
                                                                                                 cccccGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTAGCCACTTCCAGGCGCGCCCTGCGGGCCGTTGCTGGCGCAACGATCTAtcgaatcgc                                                      >  1:2671046/1‑150 (MQ=18)
                                                                                                    ccGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCg                                                   >  2:10017349/1‑150 (MQ=255)
                                                                                                     cGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGa                                                  <  1:2555335/150‑1 (MQ=255)
                                                                                                     cGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGa                                                  <  2:4632776/150‑1 (MQ=255)
                                                                                                     cGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGa                                                  <  1:3412017/150‑1 (MQ=255)
                                                                                                          gTTCTCATCCTCATGAGCTGCAGATACAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGa                                             <  2:7723244/150‑1 (MQ=255)
                                                                                                           ttCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGaa                                            <  1:9447764/150‑1 (MQ=255)
                                                                                                           ttCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACGCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGaa                                            <  2:3949376/150‑1 (MQ=255)
                                                                                                             ctcATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAAcc                                          >  1:1795591/1‑150 (MQ=255)
                                                                                                                aTCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACGTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTcc                                       >  2:7836914/1‑150 (MQ=255)
                                                                                                                          gCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCgg                             <  1:3994026/150‑1 (MQ=255)
                                                                                                                          gCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCgg                             <  2:1129049/150‑1 (MQ=255)
                                                                                                                            tGCAGTTGGAAAAAAGCCTGCTCGTTGATCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCATCGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAg                           <  2:9265586/150‑1 (MQ=255)
                                                                                                                            tGCAGATGCAAAAAAGCCGGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAg                           <  1:303796/150‑1 (MQ=255)
                                                                                                                              cAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGt                         >  2:1572221/1‑150 (MQ=255)
                                                                                                                                gATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTtc                       <  1:6354520/150‑1 (MQ=255)
                                                                                                                                  tGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGTGTCGAACCTCCCTGCCCCCGGAGGTtctc                     >  2:5326394/1‑150 (MQ=255)
                                                                                                                                   gCAAAAAAGCCTGCTGGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCAATTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTtcgac                    <  1:10891451/150‑4 (MQ=255)
                                                                                                                                    cAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTtctc                     <  2:6183863/148‑1 (MQ=255)
                                                                                                                                     aaaaaaGCCTGCTCGTTGAGCAGGCTTTTCGACTTTGGTCGGTGATAGAGGATTCCTCGCCACTACGTGGCTCTCCCTGGGGGCCGTTGCTGGCGGATCGTTCTCTGGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATc                  <  2:8669205/150‑1 (MQ=37)
                                                                                                                                     aaaaaaGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGTGTCGAACCTCCCTGCCCCCGGAGGTTCTCATc                  >  1:7620118/1‑150 (MQ=255)
                                                                                                                                     aaaaaaGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATc                  <  2:4111744/150‑1 (MQ=255)
                                                                                                                                     aaaaaaGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATc                  >  2:379966/1‑150 (MQ=255)
                                                                                                                                     aaaaaaGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATc                  >  2:7898200/1‑150 (MQ=255)
                                                                                                                                     aaaaaaCCCTGCTCGTTGAGCAGGCTTTTCGAAATTGGTCGGTGATAGAGGATTACACGCCACTTCCTGGCTCGCCCTGCGGGCCGTTGCTGGCGCACCGTTATCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATc                  <  2:7234509/150‑1 (MQ=38)
                                                                                                                                      gaaaaGCCTGCTCAACGAGCAGGCTTTTTGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCtcg                                                            >  2:2880097/2‑107 (MQ=37)
                                                                                                                                      aaaaaGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATcc                 >  1:6982008/1‑150 (MQ=255)
                                                                                                                                       aaaaGCCTGCTCAACGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCAc                                                                                                         >  1:10471377/1‑61 (MQ=17)
                                                                                                                                         aaGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCa              >  1:3655743/1‑150 (MQ=255)
                                                                                                                                            ccTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCACGCTTCGCTCGAGTCGAACCTCCCAGCCCCCGCAGCTTCTCATCCTCATGa           >  2:5928923/1‑150 (MQ=255)
                                                                                                                                               gCTCGTTTAGCAGGCTTTTCGAATATGGTCGGTGATAGAGGATTAATAGCCACTTAGTGGCTCGCCCTGCGGGCCGTTTGTGGCGCAACGTTCTCTCGCATCGCTCGACTCGAACCTCCCACCCCCCGGAGGTTCTCATCCACATGAGCt        >  1:10157039/1‑150 (MQ=255)
                                                                                                                                                cTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTg       <  1:8339034/150‑1 (MQ=255)
                                                                                                                                                cTCGGTGAGCAGGCTTTTCGAATGTGGTCGGTGATAGAGGATAACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGGACCGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTg       <  1:1315886/150‑1 (MQ=38)
                                                                                                                                                 tCGTTGAGCTGGCTTTTCGTATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGc      <  2:6620048/150‑1 (MQ=255)
                                                                                                                                                 tCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGc      <  2:10359682/150‑1 (MQ=255)
                                                                                                                                                  cGTGGAGCAGGCTTATCGAATCTGCGCGGAGAGAGAGGATCACTCGCCACTTCGTGGCTCGCCCAGCGGGCCGTTGCTGGCTCTTCGTTATCTGGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCa     <  2:702825/150‑1 (MQ=25)
                                                                                                                                                   gTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAg    >  2:4984417/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AATAAGGCGGTCGCGCCGCATCCGACAATGGCCGGCACATTTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGAT  >  NC_000913/3708280‑3708578

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: