Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 238,507 G→T 8.5% S77* (TCA→TAA)  ykfM ← lethality reduction protein, putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913238,5070GT 8.5% 1344.0 / 15.2 466S77* (TCA→TAA) ykfMlethality reduction protein, putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (234/170);  new base T (17/44);  total (251/215)
Fisher's exact test for biased strand distribution p-value = 1.38e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 476 total aligned reads displayed.

CGCAGGGGTGATAATTAGTATTGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAA  >  NC_000913/238358‑238656
                                                                                                                                                     |                                                                                                                                                     
  cAGGGGTGATAATTAGTATTGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGAt                                                                                                                                                     <  1:2048414/150‑1 (MQ=255)
   aGGGGTGATAATTAGTATTGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGAtt                                                                                                                                                    <  2:9437996/150‑1 (MQ=255)
      ggTGATAATTAGTATTGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTtta                                                                                                                                                 <  1:9208721/150‑1 (MQ=255)
      ggTGATAATTAGTATTGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTtta                                                                                                                                                 <  2:6437599/150‑1 (MQ=255)
      ggTGATAATTAGTATTGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTtta                                                                                                                                                 >  2:3396417/1‑150 (MQ=255)
        tGATAATTAGTATTGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTttatt                                                                                                                                               <  1:976834/150‑1 (MQ=255)
         gATAATTAGTATTGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTttatta                                                                                                                                              >  1:4936001/1‑150 (MQ=255)
         gATAATTAGTATTGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTttatta                                                                                                                                              >  2:7341799/1‑150 (MQ=255)
          aTAATTAGTATTGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTtattat                                                                                                                                             >  1:7441233/1‑150 (MQ=255)
             aTTAGTATTGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGGTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCgt                                                                                                                                          >  2:8408667/1‑150 (MQ=255)
             aTTAGTATTGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATTATTTTATTATctt                                                                                                                                          >  1:10774912/1‑148 (MQ=255)
             aTTAGTAATGACAAATCCTGTGACAAAAGGAGCTATTAAACGTGCAATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCgt                                                                                                                                          <  2:9092504/150‑1 (MQ=255)
                aGTATTGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCgtaag                                                                                                                                       <  1:2260405/150‑1 (MQ=255)
                 gTATTGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCgtaagt                                                                                                                                      >  2:541127/1‑150 (MQ=255)
                  tATTGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCgt                                                                                                                                          >  1:1045108/1‑145 (MQ=255)
                    ttGACATATCCGGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTaaa                                                                                                                                   >  2:2114356/1‑150 (MQ=255)
                       aCATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAAtt                                                                                                                                >  2:649827/1‑150 (MQ=255)
                       aCATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAAtt                                                                                                                                >  2:6657759/1‑150 (MQ=255)
                         atatCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTAc                                                                                                                              >  2:2034821/1‑150 (MQ=255)
                         atatCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTAc                                                                                                                              <  1:8943597/150‑1 (MQ=255)
                          tatCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACa                                                                                                                             <  1:595287/150‑1 (MQ=255)
                          tatCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACa                                                                                                                             <  2:3033594/150‑1 (MQ=255)
                          tatCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACa                                                                                                                             <  1:6269365/150‑1 (MQ=255)
                           atCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACAt                                                                                                                            <  1:3109598/150‑1 (MQ=255)
                                   aCAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATAttt                                                                                                                    >  2:8977767/1‑150 (MQ=255)
                                     aaaaGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTaa                                                                                                                  >  2:7640304/1‑150 (MQ=255)
                                     aaaaGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTaa                                                                                                                  >  1:1711066/1‑150 (MQ=255)
                                     aaaaGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTaa                                                                                                                  >  2:4143870/1‑150 (MQ=255)
                                      aaaGGATCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTaaa                                                                                                                 <  1:7010018/150‑1 (MQ=255)
                                          gAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAgg                                                                                                             >  2:8744287/1‑150 (MQ=255)
                                          gAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAgg                                                                                                             <  2:3656520/150‑1 (MQ=255)
                                           aGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGa                                                                                                            <  2:8234901/150‑1 (MQ=255)
                                             cTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGaaa                                                                                                          >  2:9182171/1‑150 (MQ=255)
                                              tATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGTaaaa                                                                                                         >  2:10108012/1‑150 (MQ=255)
                                                ttAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTAAATGAATATTTAAAAATGAAAACg                                                                                                       >  1:3794306/1‑150 (MQ=255)
                                                   aaGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACa                                                                                                    <  2:10311316/150‑1 (MQ=255)
                                                     ggTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATTATTTTATTATAGTAAGTAAAATTACATGAATATTTAAAAATGAAAACGACATg                                                                                                  >  1:9326182/1‑150 (MQ=255)
                                                        gCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGaaa                                                                                               >  2:2751431/1‑150 (MQ=255)
                                                         cTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTTTGTATGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAAc                                                                                              <  2:5570204/150‑1 (MQ=255)
                                                           aTTACGATAGCTTTTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCg                                                                                            <  2:2498992/150‑1 (MQ=255)
                                                            ttACTATAGATATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGa                                                                                           <  2:7984608/150‑1 (MQ=255)
                                                                gATAGCTATTATTAAAAATATAAGAGTTAGCTGTATTTTTATGTCTGTGGCTAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGca                                                                                       <  2:3291346/150‑1 (MQ=255)
                                                                 aTAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATTAATATTTAAAAAGGAAAACGACATGAAACCGAAGcac                                                                                      >  1:412252/1‑150 (MQ=255)
                                                                 aTAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGcac                                                                                      >  2:10940124/1‑150 (MQ=255)
                                                                 aTAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATAGTAAGTAAAATTAAATGAATATTTAAAAAGGAAAAAGACATGAAACCGAAGcac                                                                                      >  1:1285289/1‑150 (MQ=255)
                                                                  tttatattatTAAAAATATAAGTGTTAGCTGTTTTTTTATTTCTTTGGCTAAATTGACTACCTTCTTTTTTTTTATTAAGAATTATTTTATTATCGTAATTAAAATTACATGAATTTTTAAAAAGGAAAACGACATGAAACCGAAGcaca                                                                                     <  2:1873061/146‑1 (MQ=255)
                                                                     cTATTATTAAAAATATAAGAGTTAGCTTTTTTTTTATGTCTGTGGCTAAATTGACTACCTTCTTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGaa                                                                                  <  2:2277045/150‑1 (MQ=255)
                                                                     cTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATAGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGaa                                                                                  >  2:7484300/1‑150 (MQ=255)
                                                                         tAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTTTTATCGTAAGTAAAATTAAATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCaa                                                                              <  2:8597468/150‑1 (MQ=255)
                                                                            tAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTaaaaa                                                                                                               >  1:356287/1‑114 (MQ=255)
                                                                            tAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACAt                                                                           >  2:6979109/1‑150 (MQ=255)
                                                                              aaaaTATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAATAATGATTTTATTATAGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATtc                                                                         >  1:8387206/1‑150 (MQ=255)
                                                                               aaaTATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATtct                                                                        >  1:1136590/1‑150 (MQ=255)
                                                                                attataTGATTTCGTTGTTTTTTTTTGTCTGTGTCTAATTTGACTACCTTCTTTTTTTTTATTATTATTTATTTTATTTTCTTAATTAAAATTACATTAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATtctc                                                                       <  1:3396417/148‑1 (MQ=255)
                                                                                aaTATAAGTGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTAAATGAATATTTAAAAATGAAAACGACATGAAACCGAAGCACAGAATCAACATtctc                                                                       <  2:5816898/150‑1 (MQ=255)
                                                                                aaTATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATtctc                                                                       <  2:6863696/150‑1 (MQ=255)
                                                                                  tataAGAGTTTGCTTTTTTTTTTTGTCTGTGGCTAATTTTACTACCTTGTTTTTTTTGTTTAATAATTATTTTATTATCTTAATTAAAATTACATTAATATTTAAAATGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCa                                                                     <  1:4466393/150‑1 (MQ=255)
                                                                                    taAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGTTTAAGAATTATTTTATTATCGTAAGTAAAAATACAAGAATATTTAAAAATGAAAAAGACAAGAAACCGAAGCACAAAATCAACATTCTCCaaa                                                                   >  1:8795114/1‑149 (MQ=255)
                                                                                      agagTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCa                                                                 <  2:9539876/150‑1 (MQ=255)
                                                                                               tGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACAAGAATATTTAAAAAGGAAAACGACATGAAAACCAATCACAGAATAAACATTCACCAAACATAAAAAAtt                                                        >  1:7257128/1‑150 (MQ=255)
                                                                                                 tATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATTATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTc                                                      <  2:1300809/150‑1 (MQ=255)
                                                                                                  aTTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTcc                                                     >  2:387590/1‑150 (MQ=255)
                                                                                                     gTTATTTCTGTGGCTAAATTGAATACCTTCGTTTTTTTGATTAAGAATTATTTTATTATCTTAAGTAAAATTACTTTAATTTTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTg                                                  <  2:1919236/150‑1 (MQ=255)
                                                                                                     gTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTg                                                  >  2:10634675/1‑150 (MQ=255)
                                                                                                      ttATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTTTTTAAGAATTATTTTATTATAGTAAGTAAAATTAAATGAATATTTAAAAAGGAAAAAGACAAGAAAACCCAGCACAGAAAAAACAAACTCCAAAAAAAAAATAATTACGTgg                                                 >  1:9922649/1‑150 (MQ=255)
                                                                                                       tATGTCTGTGGCTAAATTGACTACCTGCGTTTTTTTGATTAAGATTGATTTTATTATCGTAATTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGa                                                <  1:10006486/150‑1 (MQ=255)
                                                                                                        aTGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACAAGAAACCGAAGCACAGAATCAACATTATACAATCATAAAATATTTCCGTGGAg                                               >  2:3763225/1‑150 (MQ=255)
                                                                                                           tCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATTAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCAt                                            <  1:541127/150‑1 (MQ=255)
                                                                                                            cTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCAtt                                           >  2:1381499/1‑150 (MQ=255)
                                                                                                             tgtgGCGAAATTGACTACCTTCGTTTTTTTTTTTAAGAATGATTTTATTATAGTAAGTTAAATTACAAGGATATTTAAAAAGGAAAACGACCTGAAACCGAAGCACCGAATAAAAAATCTACCATCCTAAAATATTTGCGTGGGGCAttt                                          >  1:8071954/1‑150 (MQ=255)
                                                                                                             tgtgGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCAttt                                          >  2:7830462/1‑150 (MQ=255)
                                                                                                             tgtgGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAAACATAAAATAATTCCGTGGAGCAttt                                          >  2:3536942/1‑150 (MQ=255)
                                                                                                               tgGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTtta                                        <  2:5296018/150‑1 (MQ=255)
                                                                                                                    aatttGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTattatt                                   <  1:7809381/147‑1 (MQ=255)
                                                                                                                     aaTTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTg                                  <  2:6340309/150‑1 (MQ=255)
                                                                                                                      aTTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAATTAAAATTAAATTAATATTAAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGa                                 <  2:4812326/150‑1 (MQ=255)
                                                                                                                       ttGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGaa                                <  2:9796585/150‑1 (MQ=255)
                                                                                                                       ttGACTACATTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGaa                                <  1:281320/150‑1 (MQ=255)
                                                                                                                             cccTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATaga                          <  1:2583573/149‑1 (MQ=255)
                                                                                                                              ccTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATagag                         <  2:5014230/150‑1 (MQ=255)
                                                                                                                              ccTTCGTTTTTTTGATTAACAATGATTTTATTATCGTAATTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATagag                         <  2:10774912/150‑1 (MQ=255)
                                                                                                                                ttCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGt                       >  2:4680792/1‑150 (MQ=255)
                                                                                                                                ttCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGt                       >  2:7235503/1‑150 (MQ=255)
                                                                                                                                  ctttttttttATTAACAATTATTTTATTAACGTAATTAAAATTACATGAATATTAAAAAAGCAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGttt                     <  1:5662128/148‑1 (MQ=255)
                                                                                                                                     ttttttGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAATAGGATATCGACATGAATCCGAAGCTCAGAATCAACATTCTCCAATCATAAAATATTTGGGTGGAGCATTTTTTTATTGTATTTTGTGGtttttg                  >  2:8854388/1‑147 (MQ=255)
                                                                                                                                         ttGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCg              >  2:3221444/1‑150 (MQ=255)
                                                                                                                                            aTTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTa           >  2:6795063/1‑150 (MQ=255)
                                                                                                                                            aTTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTa           >  1:6093219/1‑150 (MQ=255)
                                                                                                                                            aTTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATATTATTATTGAATATAGAGGTTTAACTCCggag           >  1:6514477/1‑148 (MQ=255)
                                                                                                                                             ttAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTtattat                                    <  1:8258566/124‑1 (MQ=255)
                                                                                                                                              tAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTaaa         <  1:573380/150‑1 (MQ=255)
                                                                                                                                               aaGAATGATTTTATTATCGTAAGTAAAATTACATTAATATTTAAAAATGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTaaaa        <  2:8311121/150‑1 (MQ=255)
                                                                                                                                               aaGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTaaaa        >  2:1581117/1‑150 (MQ=255)
                                                                                                                                                  aaTGATTTTATTATCTTAATTAAAATTACATGAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAc     <  1:10660253/150‑1 (MQ=255)
                                                                                                                                                  aaTGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAc     >  1:8535495/1‑150 (MQ=255)
                                                                                                                                                   aTGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACa    <  2:9333086/150‑1 (MQ=255)
                                                                                                                                                    tGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACaa   >  1:9469881/1‑150 (MQ=255)
                                                                                                                                                     gATTTTATTATCGTAATTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACaaa  <  1:465941/150‑1 (MQ=255)
                                                                                                                                                     gATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACaaa  >  1:6649791/1‑150 (MQ=255)
                                                                                                                                                     gATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACaaa  <  2:4530996/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CGCAGGGGTGATAATTAGTATTGACATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAA  >  NC_000913/238358‑238656

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: