Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 238,532 C→A 7.3% V69L (GTA→TTA)  ykfM ← lethality reduction protein, putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913238,5320CA 7.3% 1354.1 / 10.6 474V69L (GTA→TTA) ykfMlethality reduction protein, putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (230/189);  new base A (26/28);  total (257/217)
Fisher's exact test for biased strand distribution p-value = 3.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 483 total aligned reads displayed.

ATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGAAAAATA  >  NC_000913/238383‑238681
                                                                                                                                                     |                                                                                                                                                     
atatCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATTATTTTATTATCGTAATTAAAATTAc                                                                                                                                                       >  2:10130443/1‑150 (MQ=255)
atatCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTAc                                                                                                                                                       <  1:4527319/150‑1 (MQ=255)
atatCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTAc                                                                                                                                                       <  2:6759657/150‑1 (MQ=255)
 tatCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACa                                                                                                                                                      <  1:6269365/150‑1 (MQ=255)
  atCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACAt                                                                                                                                                     <  1:3109598/150‑1 (MQ=255)
     cTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGaa                                                                                                                                                  <  2:2352404/150‑1 (MQ=255)
      tgtgACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAat                                                                                                                                                 >  1:10958155/1‑150 (MQ=255)
           cAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTa                                                                                                                                            <  1:4720936/150‑1 (MQ=255)
           cAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTa                                                                                                                                            <  2:3977539/150‑1 (MQ=255)
            aaaaGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTaa                                                                                                                                           >  1:10541736/1‑150 (MQ=255)
             aaaGGATCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTaaa                                                                                                                                          <  1:7010018/150‑1 (MQ=255)
              aaGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTaaaa                                                                                                                                         <  1:2407006/150‑1 (MQ=255)
                ggAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAg                                                                                                                                       >  2:4346959/1‑150 (MQ=255)
                ggAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAg                                                                                                                                       >  2:7281347/1‑150 (MQ=255)
                ggAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATAGTAAGTAAAATTACATGAATATTTAAAAAg                                                                                                                                       >  1:3608911/1‑150 (MQ=255)
                ggAGCTATTAAAGATGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAg                                                                                                                                       >  2:9646603/1‑150 (MQ=255)
                 gAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAgg                                                                                                                                      >  1:4399035/1‑150 (MQ=255)
                 gAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAgg                                                                                                                                      <  1:7360492/150‑1 (MQ=255)
                  aGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGa                                                                                                                                     >  2:7531451/1‑150 (MQ=255)
                  aGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGa                                                                                                                                     <  2:8467954/150‑1 (MQ=255)
                   gCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGaa                                                                                                                                    >  2:219921/1‑150 (MQ=255)
                    cTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGaaa                                                                                                                                   >  2:6465076/1‑150 (MQ=255)
                            ggTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATg                                                                                                                           >  1:9400302/1‑150 (MQ=255)
                               gCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGaaa                                                                                                                        >  2:2751440/1‑150 (MQ=255)
                                 tATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAAcc                                                                                                                      >  2:10480649/1‑150 (MQ=255)
                                    tACGATAGCTATTAGTAAAAATATAAGATTTAGCTGTATTGTTTTTTCTGTGGCGAAATTGACTACCTTCTTTTTTTTGATTAAGAATTATTTTATTATCGTAATTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGaa                                                                                                                   <  2:5668304/150‑1 (MQ=255)
                                        aTAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGcac                                                                                                               <  2:8764985/150‑1 (MQ=255)
                                            cTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGaa                                                                                                           <  2:9794121/150‑1 (MQ=255)
                                             tATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAATGAAAACGACATGAAACCGAAGCACAGAAt                                                                                                          >  2:6543292/1‑150 (MQ=255)
                                             tATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAAt                                                                                                          >  2:10884954/1‑150 (MQ=255)
                                              aTTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATc                                                                                                         >  1:9472431/1‑150 (MQ=255)
                                                    aaaaaTATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACAtt                                                                                                   >  2:5855508/1‑150 (MQ=255)
                                                     aaaaTATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAATAATGATTTTATTATAGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATtc                                                                                                  >  1:8387206/1‑150 (MQ=255)
                                                     aaaaTATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATtc                                                                                                  >  1:5341920/1‑150 (MQ=255)
                                                     aaaaTATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACAAtc                                                                                                  >  1:1287602/1‑150 (MQ=255)
                                                      aaaTATATGATTTCGCTTTTTTTTTTTGTGTGTGGCTAAATTGACTACCTTCTTTTTTTTTATTAATATTGATTTTTTTATCTTATTTAAAATTAATTGAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATtct                                                                                                 <  1:6038675/150‑1 (MQ=255)
                                                       attataATATTTAGCTGTATTGTTATGTATGTGTAGAATTTGACTACCTTCTTTATTTTGATTAAGAATTATTTTTTTATCGTAATTAATATTACATTAATATTTATAATGGAAAACGACGTGAAACCGAAGCACAGAATCAACATtctc                                                                                                <  2:2593458/148‑1 (MQ=255)
                                                       aaTATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTTACTACCTTCGTTTTTTTGATTAATAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATtctc                                                                                                <  2:9355316/150‑1 (MQ=255)
                                                       aaTATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATtctc                                                                                                <  2:7797916/150‑1 (MQ=255)
                                                       aaTATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATtctc                                                                                                <  2:6863696/150‑1 (MQ=255)
                                                          ataAGAGTTAGCTTTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCaa                                                                                             <  2:112961/150‑1 (MQ=255)
                                                          ataAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATAGTAAGTAAAATTAAATGAATATTTAAAAAGTAAAAAGACATGAAACAGAATAACAGAATCAAAATTATCaaa                                                                                             >  2:8680946/1‑150 (MQ=255)
                                                                 ttAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAAAGATTTTATTATAGTAAGTAAAATTACATGAATAATTAAAAAGGAAAACGACATGAAACCCAAGCACAGAAACAACAATATACAATCATaaa                                                                                      >  1:6098370/1‑150 (MQ=255)
                                                                       gTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTTTTTAATAAATATTTTTTTATAGTAAGTAAAATTAAATTAATATTTTAAAAGGAAAAAGAAATGTAACACCAGCACACAAACAACATAATACAAAAAAAAAATAttt                                                                                >  1:2063408/1‑150 (MQ=255)
                                                                        tATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGGTTAAGAATGATTTTATTATAGTAAGTAAAATTAAATGAATATTTAAAAAGGAAAAAGACATTAAAACCAAGCACAGAAAAAACAAACTCCAAAAATAAAAAAATTc                                                                               >  1:3953760/1‑150 (MQ=255)
                                                                        tATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTc                                                                               >  2:7070898/1‑150 (MQ=255)
                                                                           tGTTATGTCTGTGGCTAAATTGACTACCTTCTTTTTTTTGATTAAGAATTATTTTATTATCGTAAGTAAAATTAAATTAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGt                                                                            <  1:7986140/150‑1 (MQ=255)
                                                                            gTTATTTCTGTGGCTAAATTGAATACCTTCGTTTTTTTGATTAAGAATTATTTTATTATCTTAAGTAAAATTACTTTAATTTTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTg                                                                           <  2:1919236/150‑1 (MQ=255)
                                                                              tttGTCTGTGGCTAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGa                                                                         <  2:3832351/148‑1 (MQ=255)
                                                                              tATGTCTGTGGCTAATTTGTCTACCTTCTTTTTTTTGTTTAATAATTATTTTATTATCTTAATTATAATTAAATTAATATTTTAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGa                                                                         <  1:6495504/150‑1 (MQ=255)
                                                                              tATGTCTGTGGCTAAATTGACTACCTGCGTTTTTTTGATTAAGATTGATTTTATTATCGTAATTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGa                                                                         <  1:10006486/150‑1 (MQ=255)
                                                                               ttGTCTGTGGCTAATTTTACTACCTTATTTTTTTTTATTAATATTGATTTTATTATCGTAATTAAATTTAAATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAg                                                                        <  1:8932226/149‑1 (MQ=255)
                                                                                  tCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCAt                                                                     >  2:2945832/1‑150 (MQ=255)
                                                                                   cTGTGGCGAAATTTACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCAtt                                                                    <  1:10838125/150‑1 (MQ=255)
                                                                                   cTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCAtt                                                                    >  2:1883032/1‑150 (MQ=255)
                                                                                   cTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCAtt                                                                    >  2:9107700/1‑150 (MQ=255)
                                                                                    tgtgGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAAACATAAAATAATTCCGTGGAGCAttt                                                                   >  2:3536942/1‑150 (MQ=255)
                                                                                      tgGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTtta                                                                 <  2:4998979/150‑1 (MQ=255)
                                                                                      tgGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACAAGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTtta                                                                 >  2:5167331/1‑150 (MQ=255)
                                                                                       ggCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTttat                                                                >  2:825123/1‑150 (MQ=255)
                                                                                       ggCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTttat                                                                >  2:5369163/1‑150 (MQ=255)
                                                                                        gCGAAATTGACTACCTTCGTTTTTTTGTTTAATAATTATTTTTTTATAGTAAGTAAAAATAAATGAAAATTTAAAAATGAAAAAGAAAATAAAAAGAAGCACAGAAAAAAAAATATCAAAAAAAAAAAAATTTAAGGGGAGCGTTttatt                                                               >  1:8252257/1‑150 (MQ=255)
                                                                                        gCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTttatt                                                               >  2:7370052/1‑150 (MQ=255)
                                                                                         ctaaaTTTAATACCTTCTTTTTTTTGATTAAGAATTATTTTATTATCTTAAGTAAATTTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTttatta                                                              <  2:3735267/148‑1 (MQ=255)
                                                                                         cGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTttatta                                                              >  2:1710704/1‑150 (MQ=255)
                                                                                           aatttGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTattatt                                                            <  1:7809381/147‑1 (MQ=255)
                                                                                           aaaTTGACTACATTCGTTTTTTTTATTAAGAATGATTTTATTATCGTATTTAAAATTACTTTATTATTTAAAATGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCTATCATAAAATATTTCCGTGGAGCATTTTattatt                                                            <  1:1842614/150‑1 (MQ=255)
                                                                                            aaTTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTg                                                           <  2:6340309/150‑1 (MQ=255)
                                                                                              ttGACTACCTTCTTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGaa                                                         <  2:2063408/150‑1 (MQ=255)
                                                                                              ttGACTACATTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGaa                                                         <  1:281320/150‑1 (MQ=255)
                                                                                                gACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATAGTAAGTAAAATTAAATGAATATTTAAAAAGGAAAACGACATGAAAACGAAGCACAGAATAAAAATAATCCAATCATAAAATATTTAAGTGGAGCATTTTATTATTGaaaa                                                       >  2:9492659/1‑148 (MQ=255)
                                                                                                  cTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAAtata                                                     <  2:8860488/150‑1 (MQ=255)
                                                                                                     ccTTCGTTTTTTTGATTAAGAATTATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATagag                                                  <  1:9122071/150‑1 (MQ=255)
                                                                                                     ccTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATagag                                                  <  2:9006259/150‑1 (MQ=255)
                                                                                                         ctttttttttATTAACAATTATTTTATTAACGTAATTAAAATTACATGAATATTAAAAAAGCAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGttt                                              <  1:5662128/148‑1 (MQ=255)
                                                                                                               tttGATTAAGAATGATTTTATTATCTTAATTAAAATTAAATTAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTcc                                        <  1:3493679/150‑1 (MQ=255)
                                                                                                                ttGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCg                                       >  2:3221444/1‑150 (MQ=255)
                                                                                                                 tGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCgg                                      <  2:1325835/150‑1 (MQ=255)
                                                                                                                   aTTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTa                                    >  2:8381713/1‑150 (MQ=255)
                                                                                                                     tAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTaaa                                  >  1:5829552/1‑150 (MQ=255)
                                                                                                                        gAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTaataaa                               >  2:8126090/1‑150 (MQ=255)
                                                                                                                        gAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTaaaaaa                               >  2:477233/1‑150 (MQ=255)
                                                                                                                         aaTGATTTTATTATCTTAATTAAAATTACATGAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAc                              <  1:10660253/150‑1 (MQ=255)
                                                                                                                         aaTGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAc                              >  1:8535495/1‑150 (MQ=255)
                                                                                                                            gATTTTATTATCGTAATTAAAATTACATTAATATTTAAAAAGTAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACaaa                           <  1:8680946/150‑1 (MQ=255)
                                                                                                                            gATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACaaa                           >  1:6649791/1‑150 (MQ=255)
                                                                                                                            gATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACCCCGGTAAAAAACaaa                           <  2:3392192/150‑1 (MQ=255)
                                                                                                                             aTTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAaag                          >  2:1179757/1‑150 (MQ=255)
                                                                                                                              ttttATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAaaga                         >  1:2643350/1‑150 (MQ=255)
                                                                                                                              ttttATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAaaga                         >  2:9203686/1‑150 (MQ=255)
                                                                                                                              ttttATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAaaga                         >  1:993654/1‑150 (MQ=255)
                                                                                                                                        gtaagtaaAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAAt               >  2:7978162/1‑150 (MQ=255)
                                                                                                                                        gtaagtaaAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAAt               >  1:1618327/1‑150 (MQ=255)
                                                                                                                                        gtaagtaaAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAAt               <  1:7228033/150‑1 (MQ=255)
                                                                                                                                           agtaaAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTATCTCCGGTAAAAAACAAAGAAGCATTGAATGCa            >  2:4571883/1‑150 (MQ=255)
                                                                                                                                           agtaaAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCa            >  2:4912804/1‑150 (MQ=255)
                                                                                                                                             taaAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCACCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAgg          >  2:1397104/1‑150 (MQ=255)
                                                                                                                                              aaaaTTAAATTATTTTTTAAAATGCAAAACGACATTAAACCGAAGCACAGAATCAACTTTCTCCTATCTTAAAATATTTCCGTGGAGCATTTTATTTTTGAATATAGAGGTTTAACTCCTGTAAAAAACAAAGAAGCATTGAATGCAggg         <  1:54579/150‑1 (MQ=255)
                                                                                                                                                   tACATGAATATTTAAAAACGAAAACTACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGaaaaa    <  1:9753410/150‑1 (MQ=255)
                                                                                                                                                     cATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGAAAaata  <  2:10716060/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ATATCCTGTGACAAAAGGAGCTATTAAAGGTGCTATTACGATAGCTATTAGTAAAAATATAAGAGTTAGCTGTATTGTTATGTCTGTGGCGAAATTGACTACCTTCGTTTTTTTGATTAAGAATGATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGAAAAATA  >  NC_000913/238383‑238681

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: