Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 164,524 A→T 7.2% K807M (AAG→ATG)  hrpB → putative ATP‑dependent helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913164,5240AT 7.2% 1256.9 / 16.4 388K807M (AAG→ATG) hrpBputative ATP‑dependent helicase
Reads supporting (aligned to +/- strand):  ref base A (175/173);  new base T (2/35);  total (178/210)
Fisher's exact test for biased strand distribution p-value = 2.93e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 405 total aligned reads displayed.

TGCCCAAAGGCCATTACAAATCACACGAGATTTGAGCGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTC  >  NC_000913/164375‑164672
                                                                                                                                                     |                                                                                                                                                    
tGCCCAAAGGCCATTACAAATCACACGAGATTTGAGCGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGaaaaa                                                                                                                                                      <  1:757166/150‑1 (MQ=255)
tGCCCAAAGGCCATTACAAATCACACGAGATTTGAGCGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGaaaaa                                                                                                                                                      <  2:1017105/150‑1 (MQ=255)
tGCCCAAAGGCCATTACAAATCACACGAGATTTGAGCGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGaaaaa                                                                                                                                                      <  2:4358423/150‑1 (MQ=255)
 gCCCAAAGGCCATTACAAATCACACGAGATTTGAGCGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAg                                                                                                                                                     >  1:1281411/1‑150 (MQ=255)
   ccAAAGGCCATTACAAATCACACGAGATTTGAGCGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTa                                                                                                                                                   >  1:5029656/1‑150 (MQ=255)
    cAAAGGCCATTACAAATCACACGAGATTTGAGCGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTAt                                                                                                                                                  >  2:7771633/1‑150 (MQ=255)
    cAAAGGCCATTACAAATCACACGAGATGTGAGCGAGTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAATGAGATGAAAGGGCGGTATCCCAATCATGTCTGGCCGGACGACCCGGCAATTACTGCACCGACGCGACGGCCGACAAAGtct                                                                                                                                                  >  1:5285667/1‑148 (MQ=255)
     aaaGGCCATTACAAATCACACGAGATTTGAGCGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTAtt                                                                                                                                                 <  2:3117327/150‑1 (MQ=255)
     aaaCGCCATTACAAATCACACGAGATTTGAGCGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTAtt                                                                                                                                                 <  2:483758/150‑1 (MQ=255)
        ggCCATTACAAATCACACGAGATTTGAGCGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGt                                                                                                                                              <  1:3378725/150‑1 (MQ=255)
         gCCATTACAAATCACACGAGATTTGAGCGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTa                                                                                                                                             >  1:2665951/1‑150 (MQ=255)
          ccATTACAAATCACACGAGATTTGAGCGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTaa                                                                                                                                            <  2:402815/150‑1 (MQ=255)
          ccATTACAAATCACACGAGATTTGAGCGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTaa                                                                                                                                            <  1:2429683/150‑1 (MQ=255)
          ccATTACAAATCACACGAGATTTGAGCGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTaa                                                                                                                                            <  1:8929611/150‑1 (MQ=255)
          ccATTACAAATCACACGAGATTTGAGCGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTaa                                                                                                                                            <  1:7433184/150‑1 (MQ=255)
                 aaaTCACACGAGATTTGAGCGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAggttggt                                                                                                                                     <  2:1282654/150‑1 (MQ=255)
                 aaaTCACACGAGATTTGAGCGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAggttggt                                                                                                                                     >  1:8214071/1‑150 (MQ=255)
                     cacaCGAGATTTGAGCGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTtc                                                                                                                                 <  2:3594048/150‑1 (MQ=255)
                     cacaCGAGATTTGAGCGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTtc                                                                                                                                 <  2:2345782/150‑1 (MQ=255)
                     cacaCGAGATTTGAGCGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTtc                                                                                                                                 >  1:5837104/1‑150 (MQ=255)
                      acacGAGATTTGAGCGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTtct                                                                                                                                <  2:8196358/150‑1 (MQ=255)
                       cacGAGATTTGAGCGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTtctc                                                                                                                               <  1:10130637/150‑1 (MQ=255)
                       cacGAGATTTGAGCGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTtctc                                                                                                                               <  2:5124121/150‑1 (MQ=255)
                       cacGAGATTTGAGCGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTtctc                                                                                                                               >  2:6262917/1‑150 (MQ=255)
                       cacGAGATTTGAGCGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTtctc                                                                                                                               <  1:4722173/150‑1 (MQ=255)
                        acGAGATTTGAGCGACTTCTGGAAAGGAGCGTACCGTGAGGTGCACAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTtctcc                                                                                                                              >  2:2131711/1‑150 (MQ=255)
                        acGAGATTTGAGCGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAATCATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTtctcc                                                                                                                              >  2:3336572/1‑150 (MQ=255)
                        acGAGATTTGAGCGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTtctcc                                                                                                                              <  2:10014180/150‑1 (MQ=255)
                        acGAGATTTGAGCGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTtctcc                                                                                                                              >  1:9255213/1‑150 (MQ=255)
                        acGAGATTTGAGCGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACCGACGAAAAAGAATGCGTCAGGTAGTATTtctcc                                                                                                                              >  1:5802020/1‑150 (MQ=255)
                         cGAGATTTGAGCGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTtctccc                                                                                                                             >  1:51811/1‑150 (MQ=255)
                          gagaTTTGAGCGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTtctccct                                                                                                                            >  2:4118024/1‑150 (MQ=255)
                           agaTTTGAGCGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTtctccctc                                                                                                                           >  2:6849091/1‑150 (MQ=255)
                              tttGAGCGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTtctccctctcc                                                                                                                        >  1:2779456/1‑150 (MQ=255)
                              tttGAGCGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTtctccctctcc                                                                                                                        >  1:3881688/1‑150 (MQ=255)
                                  aGCGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCtgt                                                                                                                    >  2:1144940/1‑150 (MQ=255)
                                  aGCGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCtgt                                                                                                                    <  1:9342414/150‑1 (MQ=255)
                                    cGACTTCTGGAACGGAGCGTACCGTGAGGTGCAAAACGAGATGACAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGTACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTgg                                                                                                                  <  2:6650492/150‑1 (MQ=255)
                                    cGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTgg                                                                                                                  <  1:4504582/150‑1 (MQ=255)
                                    cGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTgg                                                                                                                  <  2:1105427/150‑1 (MQ=255)
                                        ttCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGgaga                                                                                                              >  1:1498320/1‑150 (MQ=255)
                                        ttCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGgaga                                                                                                              <  1:10430320/150‑1 (MQ=255)
                                        ttCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCACAGGgaga                                                                                                              <  2:8659056/150‑1 (MQ=255)
                                         tCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGagag                                                                                                             <  1:6788889/150‑1 (MQ=255)
                                         tCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCACAGGGagag                                                                                                             <  2:9548540/150‑1 (MQ=255)
                                          cTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAgg                                                                                                            >  1:3534802/1‑150 (MQ=255)
                                           tGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAggg                                                                                                           <  1:6458424/150‑1 (MQ=255)
                                             gAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAGGGATTCGTAAGGTTGGTGTTCTCCCTCTCCCTGTGGGAGAGGGcc                                                                                                         >  2:5716001/1‑150 (MQ=255)
                                              aaaGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCg                                                                                                        >  2:3057059/1‑150 (MQ=255)
                                              aaaGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCg                                                                                                        <  2:5394194/150‑1 (MQ=255)
                                               aaGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCgg                                                                                                       <  2:7223008/150‑1 (MQ=255)
                                                aGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCggg                                                                                                      <  2:933741/150‑1 (MQ=255)
                                                  gAGCGTACCGTGAGGTGCAACAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAAGACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCACAGGGAGAgggagaaaac                                                                                                    <  2:5590692/150‑8 (MQ=255)
                                                  gAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGt                                                                                                    >  2:10744086/1‑150 (MQ=255)
                                                     cGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAACCATGTCTGGCCGGACGATCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAg                                                                                                 <  2:454241/150‑1 (MQ=255)
                                                     cGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAg                                                                                                 <  2:5882638/150‑1 (MQ=255)
                                                     cGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAg                                                                                                 >  2:8003841/1‑150 (MQ=255)
                                                      gTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAgg                                                                                                <  2:3233338/150‑1 (MQ=255)
                                                      gTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAgg                                                                                                >  1:10040554/1‑150 (MQ=255)
                                                       tACCGTGTGGTGCAAAAAGAGATGAATGGGCGTTAGCCCAAACATGTCGGGCGGGACGACCCGGCAAATAGTGCACCGAGGCGACGGACGAAAAAGTATTCGTACGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAggg                                                                                               <  2:966357/150‑1 (MQ=38)
                                                         ccGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCa                                                                                             <  2:51811/150‑1 (MQ=255)
                                                          cGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGcct                                                                                            >  1:5489630/1‑148 (MQ=255)
                                                          cGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCAt                                                                                            >  2:675544/1‑150 (MQ=255)
                                                           gTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATc                                                                                           >  1:3840441/1‑150 (MQ=255)
                                                               ggTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAgcg                                                                                       >  1:1018042/1‑150 (MQ=255)
                                                                gTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAgcgc                                                                                      >  1:7764284/1‑150 (MQ=255)
                                                                    aaaaaGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTAGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCAc                                                                                  <  2:2397362/150‑1 (MQ=255)
                                                                    aaaaaGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTAGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCAc                                                                                  <  2:10361790/150‑1 (MQ=255)
                                                                      aaaGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGt                                                                                >  1:2964139/1‑150 (MQ=255)
                                                                              gAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCggg                                                                                                      <  2:2599767/120‑1 (MQ=255)
                                                                              gAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTgcacccgc                                                                        >  1:6242219/1‑148 (MQ=255)
                                                                              gAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGGgcacccgc                                                                        >  2:6443313/1‑148 (MQ=255)
                                                                                       ttATCCCAAACATGTCGGGCCGCACGACCCGGAAAATACTGCACCGACGCGACGGACGAAAATGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAAcc                                                               <  2:1695908/150‑1 (MQ=255)
                                                                                       ttATCCCAAACAGGTCTGGCCGGACGACCCGGCAAATACGGCCCCGAGGCTAGTGACTAAAATTTTTTCTTATGTTTGTTTTTCTCCCTACCCCGGTGGGTGCGGGCCGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAAcc                                                               <  2:293729/150‑1 (MQ=255)
                                                                                           cccAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTGGCCCACCCCCAAAccaaca                                                           >  1:9552340/1‑146 (MQ=38)
                                                                                             cAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGGTCACCCACCCCCAAAcccacaac                                                         >  1:6112602/1‑145 (MQ=38)
                                                                                             cAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGGCCCCCACCCAAAacccacac                                                         >  2:7402179/1‑145 (MQ=37)
                                                                                               aaCATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTgg                                                                                                                  >  1:7031130/1‑91 (MQ=255)
                                                                                               aaCATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGGGAGGGCCTCAGCGCGCACGTTCCCCCACCCCCAAACCCACAccca                                                       >  1:1707669/1‑149 (MQ=38)
                                                                                                   tGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGAAAGTTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAg                                                   <  2:9084564/150‑1 (MQ=255)
                                                                                                     tCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTc                                                                                                                                                <  1:6637318/55‑1 (MQ=255)
                                                                                                       tGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCACACCCGAACCCACACCCACAAGGgag                                               >  1:9825867/1‑150 (MQ=38)
                                                                                                       gggccggccGCCCCGGCAAATACGGCCCCGCCGCGCCGGCCGAACAAGTATTCGTACGGTGGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGCGGGCCTCCGCGCGCCCGTTCACCCTCACCCTAACCCTCTCCCTTGAGGgag                                               <  2:10037604/149‑1 (MQ=25)
                                                                                                         gCCGGACGACCCGGAAAATACTGCACCGACGCGCCGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGTGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGagag                                             <  2:7060138/150‑1 (MQ=255)
                                                                                                          ccGGACGACCCGGCAAATACTGCACCGACGCGACGGACTAAAAAGTATTCGTACGGTTGGTTTTCTCCCTCTCCCTGTGGGCGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTTGAGGGAGAgg                                            <  1:9879184/150‑1 (MQ=38)
                                                                                                           cGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGGTCACCCGCACCCACACCCACACCCCCCAGGGAGAggg                                           >  1:4366184/1‑150 (MQ=37)
                                                                                                              acgacCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCAGCAGCGCGCACGTGGGCCCGCACCCCAACCCACACCCACAAGGGGGAgggggg                                        >  1:10818156/1‑148 (MQ=37)
                                                                                                              acgacCCGGAAAATACTGCCCCGCGGCGACGGACTAAAATTTATTCGTATGTTTGTTTTTCTCCCTCTCCCTGTGGGTGTGGGCGGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGAc                                        <  2:5997348/150‑1 (MQ=255)
                                                                                                                gacCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGACCCCCCCCCGAAACCCCAACCCCAAGGGGGTGGGGGCCg                                      >  1:1865563/1‑150 (MQ=25)
                                                                                                                                cacccccgcgacgGACGAAAATTTATCCTCATGGTTTTTTAACCCCCTAGCCCTGTGTTGGAGGGCCGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAAtttt                      <  1:4045932/144‑1 (MQ=255)
                                                                                                                                cACCGCCGCGCCGGAAGAAAAAGTATTCTTATGGTTGGTTTTCCCCCCCTCCCTGTGGGAGCGGGCCGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAAtttt                      <  1:221568/150‑1 (MQ=255)
                                                                                                                                  ccgccgCTAGGGACTAAAATGTATTCGTATTGTTGGTTTCCTCCCTCTCCGTGGGGGTGTGGGCGGGGGGGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTga                    <  1:8513688/150‑1 (MQ=255)
                                                                                                                                  ccgccgCGCGGGACTAAAATTTTTTCGTATGGTGGGTTTTCTCCCTCTCCGTGTGGGTGTGGGCGGGGGTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTga                    <  1:7252915/150‑1 (MQ=255)
                                                                                                                                  ccgccgCGACGGACTAAAATGTTTTCGTATGTTTGTTTTTCTCCCTCTCCCTGTGGGAGTGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTga                    <  2:8476966/150‑1 (MQ=38)
                                                                                                                                  cccacgcgacgCACCAAAAAGTATTCGAAAGGTTGGTTTACACCCACCCCCTGTGGCAGAGGGCCGGGGTGAGGCAATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTga                    <  2:8796251/147‑1 (MQ=255)
                                                                                                                                  ccGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTga                    <  2:2789610/150‑1 (MQ=255)
                                                                                                                                    gcggcgacgTACTAAAAAGTTTTCTTATGGTTGTTTTCCGCCCTCCCCCGGTGGGTGTGGGCGGGGGTGTGGGCATCAGCGCGCACGGTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTgaga                  <  1:6852933/147‑1 (MQ=255)
                                                                                                                                         gagggaccAAAATTTATTCTTACGTTTGGTTTTCCCCCACCCCGGGTGGGTGGGGGCCGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGatat             <  2:1972041/147‑1 (MQ=255)
                                                                                                                                            ggacgAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCCCCCACACCCCAACCCACAACCACAAGGGACAGGGGCCCGAACGACCCCAAAATTTTGGAGATAtctt          >  2:10836548/1‑150 (MQ=21)
                                                                                                                                                    aaGTATTCGTATGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTc  <  1:7958573/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TGCCCAAAGGCCATTACAAATCACACGAGATTTGAGCGACTTCTGGAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTC  >  NC_000913/164375‑164672

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: