Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,351,228 G→C 10.9% intergenic (‑189/+179) fabI ← / ← ycjD enoyl‑[acyl‑carrier‑protein] reductase, NADH‑dependent/DUF559 family endonuclease‑related protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,351,2280GC10.9% 1612.2 / 66.8 512intergenic (‑189/+179)fabI/ycjDenoyl‑[acyl‑carrier‑protein] reductase, NADH‑dependent/DUF559 family endonuclease‑related protein
Reads supporting (aligned to +/- strand):  ref base G (175/273);  new base C (51/10);  total (228/284)
Fisher's exact test for biased strand distribution p-value = 3.02e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 530 total aligned reads displayed.

TCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACAGC  >  NC_000913/1351080‑1351377
                                                                                                                                                     |                                                                                                                                                     
tCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCag                                                                                                                                                       <  1:9214687/150‑1 (MQ=255)
tCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCaga                                                                                                                                                      <  2:1987863/150‑1 (MQ=255)
tCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCaga                                                                                                                                                      <  2:7823655/150‑1 (MQ=255)
tCAGGCGATTCGTCGTTTTAGTAAACAGTAAGAACAGATAAACGGTTATTATAATCAACCTTGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCAAAACCACTACCCaga                                                                                                                                                      >  1:7324093/1‑150 (MQ=255)
 cAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCagag                                                                                                                                                     <  2:7489229/150‑1 (MQ=255)
  aGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAgg                                                                                                                                                    >  2:1490633/1‑150 (MQ=255)
   ggCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAggg                                                                                                                                                   <  2:5128802/150‑1 (MQ=255)
       aTTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGa                                                                                                                                               <  1:5762032/150‑1 (MQ=255)
        ttCGTCGCTTTAGTAAACAGTACGAACAGATAAACGGTTATTAAAATCAACCTCGCTGTGAGTAGCTATAGATGCCAGGTCCGACCGGAGCAGCCTTCGGCAGGGGGGGCGCTTTTCCCCTCACCCAAACCCTCTCCACAGATGGGCGAg                                                                                                                                              >  2:7345201/1‑150 (MQ=255)
         tcgtcgTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAgg                                                                                                                                             <  2:7997809/150‑1 (MQ=255)
         tcgtcgTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCGCCCCTCTGGGGAGAgg                                                                                                                                             <  1:8648755/150‑1 (MQ=255)
            tcgtTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGa                                                                                                                                          <  2:343336/150‑1 (MQ=255)
            tcgtTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACACTCTCCCCAGAGGGGCGAGGGGa                                                                                                                                          <  2:3487588/150‑1 (MQ=255)
              gtTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGAcc                                                                                                                                        <  1:6527883/150‑1 (MQ=255)
              gtTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTACCCGCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGAcc                                                                                                                                        >  1:9836011/1‑150 (MQ=255)
              gtTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCAGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCACACCCAAACCCACAACACAAAAGGGCGAGGGGAcc                                                                                                                                        >  1:5965740/1‑150 (MQ=255)
                tttAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGt                                                                                                                                      >  2:8964124/1‑150 (MQ=255)
                 ttAGTAAACAGTACGAACAGATTAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTACCCCTCTCCCCAGAGGGGCGAGGGGACCGTa                                                                                                                                     <  2:8352242/150‑1 (MQ=255)
                 ttAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCAAACCCACTCCCCAGAGGGGCGAGGGGACCCTa                                                                                                                                     >  2:9685035/1‑150 (MQ=255)
                   aGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTAtt                                                                                                                                   <  1:7062541/150‑1 (MQ=255)
                     tAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCAAACCCACACCCCAGAGGGGCGAGGGGACCGTAAtgt                                                                                                                                 >  1:2173864/1‑150 (MQ=255)
                     tAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTACCCCCCACCAAAACCACACCCCCGAGGGGGGGGGGGCCCGtgttgt                                                                                                                                 >  1:4680196/1‑150 (MQ=255)
                        aCAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCCCACCCAAACCCAAAACCCACAGGGGCGCGGGGGCCGGATtgtgga                                                                                                                              >  1:4064152/1‑148 (MQ=255)
                           gTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAATAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCACACCCTAACCCACACCCCACAGGGGCCAGGGGCCCGAATTGTGCCAat                                                                                                                           >  1:9128287/1‑150 (MQ=255)
                              cGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTTTAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATAtt                                                                                                                        <  2:464481/150‑1 (MQ=255)
                              cGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCGCACCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATAtt                                                                                                                        >  1:1809783/1‑150 (MQ=255)
                               gAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTg                                                                                                                       <  2:9085210/150‑1 (MQ=255)
                                aaCAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGt                                                                                                                      <  2:2343899/150‑1 (MQ=255)
                                aaCAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGt                                                                                                                      <  2:3601537/150‑1 (MQ=255)
                                  cAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTa                                                                                                                    <  1:5601727/150‑1 (MQ=255)
                                   aGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTAc                                                                                                                   <  1:2247038/150‑1 (MQ=255)
                                    gATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTACCCACACCCAAACCCACACCCCACAGGGGCGCGGGGACCCGAGTGTGCAAATATTGTTaac                                                                                                                  >  1:7440402/1‑148 (MQ=255)
                                     aTAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCGCACCCGAACCCTCTCCCCACAGGGGCCAGGGGCCCGTCTTGTGCAAATATTGTTAccc                                                                                                                 >  2:4950929/1‑150 (MQ=255)
                                     aTAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCACACCCAAACCCACTCCCCAGAGGGGCGAGGGGACCGTAATGTGCAAATATTGTTAccc                                                                                                                 >  2:4318394/1‑150 (MQ=255)
                                      tAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTAcccc                                                                                                                <  2:7065470/150‑1 (MQ=255)
                                        aaCGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAg                                                                                                              <  2:6080347/150‑1 (MQ=255)
                                           ggTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGcaa                                                                                                           >  2:10938971/1‑150 (MQ=255)
                                              tattatAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCACACCCAAAACCTCACCCCAGAGGGGCGCGGGGACCCGATTGTGCCAAAAATGTTACCCCAGcaacaa                                                                                                        >  2:562441/1‑150 (MQ=255)
                                               attatAATCAACCTGGCTGTGAGTAGCTATAGTTGGCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCaacaaa                                                                                                       <  1:5410276/150‑1 (MQ=255)
                                                 tataATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCaacaaaca                                                                                                     <  1:9496194/150‑1 (MQ=255)
                                                  ataATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCGCACCCAAAACCACCCCCCCCACGGGCGCGGGGGCCCGGGTGTGCAAAAAATTTTACCCCCCCaacaaaaac                                                                                                    >  2:3805944/1‑147 (MQ=255)
                                                  ataATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCACACCCAAACCCACTCCCCACAGGGGCGAGGGGGCCGGAATGTGCCAAAATAGTTACCCCCCCCACAAACAg                                                                                                    >  2:9236905/1‑150 (MQ=255)
                                                    caTCAACCTGGCTGTGAGTAGCTTTAGTTGACAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCACTCACCCTAACCCTCTCCCCATAGGGGCGTGGGGACCTTATTGTGCATATATTGTTACCCCATCAACAAACTGGc                                                                                                  <  1:2541623/149‑1 (MQ=38)
                                                     aTCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCAAACCCAAAACCCCCAGGGGCGAGGGGACCGTAATGTGCAAAAAATGTTAACCCACCAACAAACAGGcg                                                                                                 >  2:8621896/1‑149 (MQ=255)
                                                     aTCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCGCCCCCCAAACCCCAACCCCCAGGGGGGCGGGGGCCCGATTGTGGCAAAAATGTTTCCCCCCCCACAAACAGGcg                                                                                                 >  1:4945364/1‑149 (MQ=255)
                                                      tCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCCGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTc                                                                                                <  2:5502834/150‑1 (MQ=255)
                                                      tCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCACACCCAAAACCACAACCCACAGGGGCGAGGGGACCGGATTTTGCAAAAATTGTTAACCCCGCAACAAAAAGGCTc                                                                                                >  1:2712722/1‑150 (MQ=255)
                                                       cAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCa                                                                                               <  1:2035974/150‑1 (MQ=255)
                                                         aCCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATa                                                                                             <  1:8232695/150‑1 (MQ=255)
                                                            tGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTACCCAAACCCAAAACCAAACCCCAGAGGGGCGAGGGGACCCTATTGTGCCAATATTTTTACCCCACCAACAAACAGGCGCaaaaag                                                                                          >  1:5875516/1‑145 (MQ=255)
                                                               cTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGccc                                                                                       <  2:8131899/150‑1 (MQ=255)
                                                                tgtgAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCGCACCCAAACCCACACCCCAGAGGGGCGAGGGGACCCTATTGTGCAAAAAATGTTAACCCACCAACAAACAGGCACATACAGcccc                                                                                      >  1:8001711/1‑150 (MQ=255)
                                                                    aGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCGCACCCAAAACCACACCCCAGAGGGGCGAGGGGACCCTAATTTGCAAAAAATTTTAACCCAGCAACAAAAAGGCACAAAAAGCCCCaaaa                                                                                  >  2:485517/1‑149 (MQ=255)
                                                                     gTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACGAACAGGCTCATACAGCCCCTAAcc                                                                                 <  1:3904747/150‑1 (MQ=255)
                                                                     gTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAAcc                                                                                 <  2:7201482/150‑1 (MQ=255)
                                                                     gTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCAAACCCACTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTAACCCAGCAAAAAACAGGCTCATACACCCCCAAAcc                                                                                 >  2:8542145/1‑150 (MQ=39)
                                                                     gTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTACCCTCACCCAAACCCTCTCCCCAGAGGGGCGGGGGGGCCGTATTTTTCAAAAATTGTTAACCCAGCAACAAAAAAGCGCATACACCCCCGAAcc                                                                                 >  1:8853894/1‑150 (MQ=255)
                                                                       tgCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCt                                                                               <  1:10584620/149‑1 (MQ=255)
                                                                          tataGGTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTc                                                                            <  1:10731870/150‑1 (MQ=255)
                                                                           ataGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTGCCCGCACCCAAAACCAAACCCCCCCGGGGCGCGGGGGCCCGAGTGTTGAAAAATTTTTAACCCACCAAAAAAAAAGCGCAAAAAACCCCCAAcccaatga                                                                           >  1:3563046/1‑145 (MQ=255)
                                                                           ataGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTGCCCAAACCCAAAACCACACCCCCCAGGGGCGCGGGGCCCCGATTGTTCAAAAATTTTTTAACCACCAACAAACAAGCGCAAAAACCCCCCAAccccaaaa                                                                           >  1:9017470/1‑145 (MQ=255)
                                                                            taGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCAAACCCTCAACCCCGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCAAACCCGATCAt                                                                          >  2:7671788/1‑150 (MQ=255)
                                                                              gTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATgg                                                                        <  1:3008307/150‑1 (MQ=255)
                                                                                 gCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGa                                                                     <  1:4338859/150‑1 (MQ=255)
                                                                                 gCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTACCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGa                                                                     >  2:10792148/1‑150 (MQ=255)
                                                                                  ccAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGAt                                                                    <  1:10192458/150‑1 (MQ=255)
                                                                                   cAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATg                                                                   <  2:2826453/150‑1 (MQ=255)
                                                                                    aGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATgg                                                                  <  2:10852504/150‑1 (MQ=255)
                                                                                    aGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCAAACCCTCTCCCCACAGGGGCGAGGGGACCAGATTGTGCAAAAATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCATACATGGCGCTgg                                                                  >  2:9885098/1‑150 (MQ=255)
                                                                                      gTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCt                                                                <  1:108963/150‑1 (MQ=255)
                                                                                                aGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTc                                                      <  1:9685035/150‑1 (MQ=255)
                                                                                                 gCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCa                                                     <  2:6259005/150‑1 (MQ=255)
                                                                                                    ggCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGAc                                                  <  1:1490633/150‑1 (MQ=255)
                                                                                                    ggCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGAc                                                  <  1:778652/150‑1 (MQ=255)
                                                                                                             aGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCGTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGaa                                         <  2:4528907/150‑1 (MQ=255)
                                                                                                                ggggggCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAata                                       <  1:1318799/150‑1 (MQ=255)
                                                                                                                 gggggCGCTTTTCCCCAAAACCAAAACCACTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACACCACCAACAAACAGGCACAAAAACCCCCAAAACCTTTCAAGGCCAAGGCGGGGACGGGTCAGACCGTGCCGAaaat                                      >  2:3087978/1‑150 (MQ=255)
                                                                                                                  ggggCACTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATAtt                                     >  2:1774856/1‑150 (MQ=255)
                                                                                                                     gcgcTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCCAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCAAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATtctc                                  >  2:2525452/1‑150 (MQ=255)
                                                                                                                     gcgcTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATtctc                                  <  2:6902183/150‑1 (MQ=255)
                                                                                                                       gcTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCa                                <  2:6504441/150‑1 (MQ=255)
                                                                                                                         ttttCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGc                              >  2:5781543/1‑150 (MQ=255)
                                                                                                                         ttttCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGc                              >  2:5886544/1‑150 (MQ=255)
                                                                                                                           ttCCCCTCACCCTAACCCACTCCCCACAGGGGCGAGGGGACCGTATTTTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCAc                            >  2:3294443/1‑150 (MQ=255)
                                                                                                                               ccTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGtc                        <  2:872790/150‑1 (MQ=255)
                                                                                                                               ccTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGGTCAGACCTTGCCGAATATTCTCCAGCACCGtc                        >  2:2228398/1‑150 (MQ=255)
                                                                                                                                cTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGtct                       <  1:4126732/150‑1 (MQ=255)
                                                                                                                                   aCCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCa                    >  1:321556/1‑150 (MQ=255)
                                                                                                                                    cccTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCAt                   <  1:1122277/150‑1 (MQ=255)
                                                                                                                                     ccTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATg                  >  2:973110/1‑150 (MQ=255)
                                                                                                                                     ccTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATg                  >  1:3374115/1‑150 (MQ=255)
                                                                                                                                     ccTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATg                  >  1:10045864/1‑150 (MQ=255)
                                                                                                                                           ccTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTca            >  1:8476596/1‑150 (MQ=255)
                                                                                                                                            ctctCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTcac           <  1:5108811/150‑1 (MQ=255)
                                                                                                                                            ctctCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTcac           >  2:10508940/1‑150 (MQ=255)
                                                                                                                                               tcCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTcaccac        >  1:2363360/1‑150 (MQ=255)
                                                                                                                                               tcCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTcaccac        >  1:2864307/1‑150 (MQ=255)
                                                                                                                                                ccccAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACcaca       <  2:10297537/150‑1 (MQ=255)
                                                                                                                                                ccccAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACcaca       >  2:4083343/1‑150 (MQ=255)
                                                                                                                                                   cAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCacaaca    <  1:7618257/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TCAGGCGATTCGTCGTTTTAGTAAACAGTACGAACAGATAAACGGTTATTATAATCAACCTGGCTGTGAGTAGCTATAGTTGCCAGGTCCGACCGGAGCAGGCTGCGGCAGGGGGGGCGCTTTTCCCCTCACCCTAACCCTCTCCCCAGAGGGGCGAGGGGACCGTATTGTGCAAATATTGTTACCCCAGCAACAAACAGGCTCATACAGCCCCTAACCCTTTCATGGCGATGGCTGGGACGGTTCAGACCTTGCCGAATATTCTCCAGCACCGTCTCCATGTTTTCACCACAACAGC  >  NC_000913/1351080‑1351377

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: