Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,636,476 T→A 9.5% intergenic (‑109/‑280) nohQ ← / → ynfO pseudogene, Qin prophage; Phage DNA packaging protein Nu1 family;Phage or Prophage Related; DNA packaging protein NU1 homolog from lambdoid prophage Qin/uncharacterized protein, Qin prophage

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,636,4760TA 9.5% 1465.2 / 24.6 496intergenic (‑109/‑280)nohQ/ynfOpseudogene, Qin prophage; Phage DNA packaging protein Nu1 family;Phage or Prophage Related; DNA packaging protein NU1 homolog from lambdoid prophage Qin/uncharacterized protein, Qin prophage
Reads supporting (aligned to +/- strand):  ref base T (171/260);  new base A (61/2);  total (234/262)
Fisher's exact test for biased strand distribution p-value = 5.62e-20
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 509 total aligned reads displayed.

CGCACCGAAAATGTCAGCCAGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGCCAAAAACAGAGAAAGGAAACGACAGAGGCCCAAAAGCTCGTTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGGGGGTATTTTAAATAAAAACATTAAGTTACGACGAAGAAGAACGGAAATGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATCACAACGTG  >  NC_000913/1636326‑1636625
                                                                                                                                                      |                                                                                                                                                      
  cACCCAAAATGACACCCACCTGCAATTTGATGACTACCATTGTACATACCACCCCCAAAAACAGAGAAAGGAAACGACAGAGGCCCAAAACCTCGTTTTCAGCACCTGTCGTTTCCTTTCATTTCAGGGGGTATTTTAAATAAAAACAtt                                                                                                                                                       <  2:8006269/150‑1 (MQ=14)
     cGAAAATGTCAGCCAGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGCCAAAAACAGAGAAAGGAAACGACAGAGGCCCAAAAGCTCGTTTTCAGCACCTGTCGTTTGCTTTCTTTTGAGGGGGGGTTTTTAAAAAAAAAAATAAg                                                                                                                                                    >  1:4736497/1‑150 (MQ=14)
     cGAAAATGTCAGCCAGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGCCAAAAACAGAGAAAGGAAACGACAGAGGCCCAAAAGCTCGTTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGGGGGTATTTTAAATAAAAACATTAAg                                                                                                                                                    >  2:5423621/1‑150 (MQ=255)
     cGAAAATGTCAGCCAGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGCCAAAAACAGAGAAAGGAAACGACAGAGGCCCAAAAGCTCGTTTTCAGCACCTGTCGTTTCCTTTATTTTCAGGGGGTATTTTAAATAAAAACATTAAg                                                                                                                                                    >  1:10898434/1‑150 (MQ=255)
        aaaTGTCAGCCAGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGCCAAAAACAGAGAAAGGAAACGACAGAGGCCCAAAAGCTCGTTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGGGGGTATTTTAAATAAAAACATTAAGTTa                                                                                                                                                 <  1:5034152/150‑1 (MQ=255)
               agccagcTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGCCAAAAACAGAGAAAGGAAACGACAGAGGCCCAAAAGCTCGTTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGGGGGTATTTTAAATAAAAACATTAAGTTACGACgaa                                                                                                                                          <  2:7490687/150‑1 (MQ=255)
               agccagcTGATTTTTGTTGACTTCCATTGTACATACCACGCCCAAAAACAGAGAAAGCAAACGACAGAGGCCCAAAAGCTCGTTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGGGGGTATTTTAAATAAAAACATTAAGTTACGACgaa                                                                                                                                          <  1:8357964/150‑1 (MQ=14)
                gccagcTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGCCAAAAACAGAGAAAGGAAACGACAGAGGCCCAAAAGCTCGTTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGGGGGTATTTTAAATAAAAACATTAAGTTACGACgaag                                                                                                                                         <  2:6409069/150‑1 (MQ=255)
                  cagcTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGCCAAAAACAGAGAAAGGAAACGACAGAGGCCCAAAAGCTCGTTTTCAGCACCTGTCGTTTGCTTTGTTTTCAGGGGGGATTTTTAATAAAAACATTAATTTACGACgaataa                                                                                                                                       >  2:2954167/1‑150 (MQ=14)
                  cagcTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGCCAAAAACAGAGAAAGGAAACGACAGAGGCCCAAAAGCTCGTTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGGGGGTATTTTAAATAAAAACATTAAGTTACGACgaagaa                                                                                                                                       <  2:3456796/150‑1 (MQ=255)
                  cagcTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGCCAAAAACAGAGAAAGGAAACGACAGAGGCCCAAAAGCTCGTTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGGGGGTATTTTAAATAAAAACATTAAGTTACGACgaagaa                                                                                                                                       <  2:3674277/150‑1 (MQ=255)
                   agcTGATTTTTGTTGACTTCCATTGTTCATTCAACGGCAAAAAACAGAGAAAGGAAACGACAGAGGCCCAAAAGCTCGTTTTCAGCACCTGTCTTTTCCTTTCTTTTCAGGGGGTATTTTAAATAAAAACATTAAGTTACGACgaagaag                                                                                                                                      <  1:6700029/150‑1 (MQ=255)
                     ctgctTTTTGTTGACTTCCATTGTTCATTCCACGGCCAAAAACAGAGAAAGGAAACGACAGAGGCCCAAAAGCTCGTTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGGGGGTATTTTAAATAAAAACATTAAGTTACGACgaagaagaa                                                                                                                                    >  2:7061957/1‑150 (MQ=255)
                      tgctTTTTGTTGACTTCCATTGTTCATTCCACGGCCAAAAACAGAGAAAGGAAACGACAGAGGCCCAAAAGCTCGTTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGGGGGTATTTTAAATAAAAACATTAAGTTACGACGAAGAAGAAc                                                                                                                                   <  2:6042906/150‑1 (MQ=255)
                      tgctTTTTGTTGACTTCCATTGTTCATTCCACGGCCAAAAACAGAGAAAGGAAACGACAGAGGCCCAAAAGCTCGTTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGGGGGTATTTTAAATAAAAACATTAAGTTACGACGAAGAAGAAc                                                                                                                                   <  1:4333848/150‑1 (MQ=255)
                        ctTTTTGTTGACTTCCATTGTTCATTCCACGGCCAAAAACAGAGAAAGGAAACGACAGAGGCCCAAAAGCTCGTTTTCAGCACCTGTCGTTTGCTTTATTTTGAGGGGGTATTTTAAATAAAAACATTTAGTTAAGACGAAGGAGAACgg                                                                                                                                 >  1:308912/1‑150 (MQ=14)
                         tttttGTTGACTTCCATTGTTCATTCCACGGCCAAAAACAGAGAAAGGAAACGACAGAGGCCCAAAAGCTCGTTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGGGGGTATTTTAAATAAAAACATTAAGTTACGACGAAGAAGAACGGa                                                                                                                                <  1:4439478/150‑1 (MQ=255)
                              gttgACTTCCATTGTTCATTCCACGGCCAAAAACAGAGAAAGGAAACGACAGAGGCCCAAAAGCTCGTTTTCAGCACCTGTCGTTTCCTTTCTTTTAAGGGGGTATTTTAAATAAAAACATTAAGTTAAGAAGAAGAAGAACGGAAATGc                                                                                                                           >  1:9455700/1‑150 (MQ=255)
                               ttgACTTCCATTGTTCATTCCACGGCCAAAAACAGAGAAAGGAAACGACAGAGGCCCAAAAGCTCGTTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGGGGGTATTTTAAATAAAAACATTAAGTTTAGACGAAGCAGAACGGAAATGcc                                                                                                                          >  2:8498520/1‑150 (MQ=255)
                               ttcaattCAATTGTACATACCACCCAAAAAAACACACAAACCAAACGACACACCCCCAAAAGCACGTTTTCAGCACCTCACGTTTCCTTTCATTACAGGGCGTATTTTAAATAAAAACATTAACTTACGACGAAGAAGAACGGAAATGcc                                                                                                                          <  2:3782606/145‑1 (MQ=2)
                                tgACTTCCATTGTTCATTCCACGGCCAAAAACAGAGAAAGGAAACGACAGAGGCCCAAAAGCTCGTTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGGGGGTATTTTAAATAAAAACATTAAGTTACGACGAAGAAGAACGGAAATGCCt                                                                                                                        <  2:5284622/150‑1 (MQ=255)
                                  aCTTCCATTGTTCATTCCACGGCCAAAAACAGAGAAAGGAAACGACAGAGGCCCAAAAGCTCGTTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGGGGGTATTTTAAATAAAAACATTAAGTTACGACGAAGAAGAACGGAAATGCCTTa                                                                                                                      <  2:6274930/150‑1 (MQ=255)
                                    ttCCATTGTTCATTCCCCGGCCAAAAACAGAGAAAGGAAACGACAGAGGCCCAAAAGCTCGTTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGGGGGTATTTTTAATAAAAAAAATAAGTTTAGACGAAGAAGAAAGGAAATTCCTTaaa                                                                                                                    >  2:1992256/1‑150 (MQ=14)
                                    ttCCATTGTTCATTCCACGGCCAAAAACAGAGAAAGGAAACGACAGAGGCCCAAAAGCTCGTTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGGGGGTATTTTAAATAAAAACATTAAGTTACGACGTAGAAGAACGGAAAATCCTTaaa                                                                                                                    >  2:3931772/1‑150 (MQ=255)
                                    ttCCATTGTTCATTCCACGGCCAAAAACAGAGAAAGGAAACGACAGAGGCCCAAAAGCTCGTTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGGGGGTATTTTAAATAAAAACATTAAGTTACGACGAAGAAGAACGGAACTGCCTTaaa                                                                                                                    >  2:6749765/1‑150 (MQ=255)
                                     tCCATTGTTCATTCCACGGCCAAAAACAGAGAAAGGAAACGACAGAGGCCCAAAAGCTCGTTTTCAGCACCTGTCGTGTGCTTTCTTTTAGGGGGGTGTTTTATATAAAAAAAATAAGTTAAGACGAAAAAGAACGGCAATACCTTaaaa                                                                                                                   >  1:5118889/1‑149 (MQ=14)
                                      caaTTGTTCATTCCACGGCCAAAAACAGAGAAAGGAAACGACAGAGGCCCAAAAGCTCGTTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGGGGGTATTTTAAATAAAAACATTAAGTTACGACGAAGAAGAACGGAAATGCCTTAAAcc                                                                                                                  <  1:10136960/148‑1 (MQ=255)
                                        aTTGTTCATTCCACGGCCAAAAACAGAGAAAGGAAACGACAGAGGCCCAAAAGCTCGTTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGGGGGTATTTTAAATAAAAACATTAAGTTACGACGAAGAAGAACGGAAATGCCTTAAACCgg                                                                                                                <  1:10028793/150‑1 (MQ=255)
                                        aTTGTTCATTCCACGGCCAAAAACAGAGAAAGGAAACGACAGAGGCCCAAAAGCTCGTTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGGGGGTATTTTAAATAAAAACATTAAGTTACGACGAAGAAGAACGGAAATGCCTTAAACCgg                                                                                                                <  1:1630793/150‑1 (MQ=255)
                                        aTTGTTCATTCCACGGCCAAAAACAGAGAAAGGAAACGACAGAGGCCCAAAAGCTCGTTTTCAGCACCTGTCGTTTACTTTCTTTTAAGGGGGTATTTTAAATAAAAAAATTAAGTTAAGACGAAGAAGAACGGAAAAGCCTTAAACCgg                                                                                                                >  1:2746250/1‑150 (MQ=14)
                                         ttGTTCATTCCACGGCCAAAAACAGAGAAAGGAAACGACAGAGGCCCAAAAGCTCGTTTTCAGCACCTGTCGTGTCCTTTCTTTTCAGGGGGGATTTTAAATAAAAACAATAAGTTAAGACCAAGAATAACGGCAATTCCATaaaaccga                                                                                                               >  2:9213773/1‑145 (MQ=14)
                                           gTTCATTCCACGGCCAAAAACAGAGAAAGGAAACGACAGAGGCCCAAAAGCTCGTTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGGGGGTATTTTAAATAAAAACATTAAGTTACGACGAAGAAGAACGGAAATGCCTTAAACCGGaaa                                                                                                             <  1:1445636/150‑1 (MQ=255)
                                            ttCATTCCACGGCCAAAAACAGAGAAAGGAAACGACAGAGGCCCAAAAGCTCGTTTTCAGCACCTGTCGTTTGCTTTATTTTAATGGGGTGTTTTAAATAAAAAAAATAATTTAAGAAGAAGAATAAAGGAAATGCCATAAAACGGaaaa                                                                                                            >  1:4055761/1‑150 (MQ=14)
                                            ttCATTCCACGGCCAAAAACAGAGAAAGGAAACGACAGAGGCCCAAAAGCTCGTTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGGGGGTATTTTAAATAAAAACATTAAGTTACGACGAAGAAGAACGGAAATGCCTTAAACCGGaaaa                                                                                                            <  2:6827904/150‑1 (MQ=255)
                                            ttCATTCCACGGCCAAAAACAGAGAAAGGAAACGACAGAGGCCCAAAAGCTCGTTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGGGGGTATTTTAAATAAAAACATTAAGTTACGACGAAGAAGAACGGAAATGCCTTAAACCGGaaaa                                                                                                            <  2:8201801/150‑1 (MQ=255)
                                                ttCCACGGCCAAAAACAGAGAAAGGAAACGACAGAGGCCCAAAAGCTCGTTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGGGGGTATTTTAAATAAAAACATTAAGTTACGACGAAGAAGAACGGAAATGCCTTAAACCGGAAAAtttt                                                                                                        <  2:5178851/150‑1 (MQ=255)
                                                ttCCACGGCCAAAAACAGAGAAAGGAAACGACAGAGGCCCAAAAGCTCGTTTTCAGCACCTGTCGTTTCCATTATTTTAAGGGGGTATTTTTAATAAAAAAAATAATTTAAGACGAATAAGAAAGGAAAAGACATAAAAAGGAAAAattt                                                                                                        >  1:4304427/1‑150 (MQ=14)
                                                 tCCACGGCCAAAAACAGAGAAAGGAAACGACAGAGGCCCAAAAGCTCGTTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGGGGGTATTTTAAATAAAAACATTAAGTTACGACGAAGAAGAACGGAAATGCCTTAAACCGGAAAATTTTc                                                                                                       <  1:2295435/150‑1 (MQ=255)
                                                 tCCACGGCCAAAAACAGAGAAAGGAAACGACAGAGGCCCAAAAGCTCGTTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGGGGGTATTTTAAATAAAAACATTAAGTTACGACGAAGAAGAACGGAAATGCCTTAAACCGGAAAATTTTc                                                                                                       <  2:8903494/150‑1 (MQ=255)
                                                 tCCACGGCCAAAAACAGAGAAAGGAAACGACAGAGGCCCAAAAGCTCGTTTTCAGCACCTGTCGTTTACTTTCTTTTCAGGGGGTATTTTAAATAAAAACATTAAGTTAAGACGAAGAAGAACCGAAATGCCTTAAACCGGAAAATTTTc                                                                                                       >  2:4252560/1‑150 (MQ=255)
                                                  ccACGGCCAAAAACAGAGAAAGGAAACGACAGAGGCCCAAAAGCTCGTTTTCAGCACCTGTCGTTTGCTTTCTGTTCAGGGGGTATTTTAAATAAAAACATTAAGTTACGACGAAGAAGAACGGCAATGCCTTAAACCGGAAAATTTTCa                                                                                                      >  2:5888598/1‑150 (MQ=255)
                                                  ccACGGCCAAAAACAGAGAAAGGAAACGACAGAGGCCCAAAAGCTCGTTTTCAGCACCTGTCGTTTCCATTCTTTTCAGGGGGTATTTTAAATAAAAAAAATAATTTAAGACGAAGAAGAACGGAAATTCCGTAAACAGGAAAAATTTCa                                                                                                      >  1:10802920/1‑150 (MQ=14)
                                                  ccACGGCCAAAAACAGAGAAAGGAAACGACAGAGGCCCAAAAGCTCGTTTTCAGCACCTGTCGTGTGCTTTCTTTTAAGGGGGTATTTTAAATAAAAAAAATAAATTAAGACGAAGAAAAAAGGAAATGCCATGTAACGGGAAAAtttaa                                                                                                       >  1:7595569/1‑148 (MQ=14)
                                                    aCGGCCAAAAACAGAGAAAGGAAACGACAGAGGCCCAAAAGCTCGTTTTCAGCACCTGTCGTTTGCTTTATTTTAAGGGGGTATTTTAAATAAAAAAAAAAATTTAACAAGAAGAATAACCGAAATTCCTTAAACCGGAAAATTTTAATa                                                                                                    >  1:2574962/1‑150 (MQ=14)
                                                     cGGCCAAAAACAGAGAAAGGAAACGACAGAGGCACAAAAGCTCGTTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGGGGGTATTTTAAATAAAAAAATTGAGTTAAAACGAAGAAGAACCGAAAAGCATTAAACCGGAAAATTTtaataa                                                                                                   >  2:7651508/1‑150 (MQ=14)
                                                         aaaaaaCATACAAACCAAACGACAGAGGCCCAAAAGCTCGTTTTCATCACCTGTCGTTTCCTTTCATTTCAGGGGGTATTTTAAAAAAAAACATTAAGTTACGACGAAGAAGAACGGAAATGCCTTAAACCGGAAAATTTTCATAAATAg                                                                                               <  1:2089057/149‑1 (MQ=14)
                                                          aaaaaCAGAGAAAGGAAACGACAGAGGCCCAAAAGCTCGTTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGGGGGTATTTTAAATAAAAACATTAAGTTACGACGAAGAAGAACGGAAATGCCTTAAACCGGAAAATTTTCATAAATAGc                                                                                              <  1:6136394/150‑1 (MQ=255)
                                                                agagAAAGGAAACGACAGAGGCCCAAAAGCTCGTTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGGGGGTATTTTAAATAAAAACATTAAGTTACGACGAAGAAGAAACGAAATGCCTTAAACCGGGAAATTTTAATAAATATCGaaaaa                                                                                        >  2:1185128/1‑149 (MQ=14)
                                                                   gaAAGGAAACGACAGAGGCCCAAAAGCTCGTTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGGGGGTATTTTAAATAAAAACATTAAGTTACGACGAAGAAGTACGGGAATGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAAACcg                                                                                     >  2:2196677/1‑150 (MQ=255)
                                                                       ggAAACGACAGAGGCCCAAAAGCTCGTTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGGGGGTATTTTAAATAAAAACATTAAGTTACGACGAAGAAGAACGGAAATGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAg                                                                                 <  2:7257592/150‑1 (MQ=255)
                                                                        gAAACGACAGAGGCCCAAAAGCTCGTTTTCAGCACCTGTCGTTTACTTTATTTTGAGGGGGTATTTTAAATAAAAACAATAAGTTACGACGAAGAAGAACGGAAAATCCGTAAAACGGAAAATTTTAATAAATAGCGAAAACCCGCGAgg                                                                                >  2:1035520/1‑150 (MQ=11)
                                                                           aCGACATAGGCCCAAAAGCTCGTTTTCAGCACCTGTCGTTTCCTTTGTTTTGAGGGGGTATTTTAAATAAAAAAATTTATTTAAGAAGAATAATTACGGAAATGGCTTAAACCGGAAAATTTTAATAAATAGCGTAAACCCGCGAGGTcg                                                                             >  2:10850759/1‑150 (MQ=14)
                                                                            cGACAGAGGCCCAAAAGCTCGTTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGGGGGTATTTTAAATAAAAACATTAAGTTACGACGAAGAAGAACGGAAATGCCTTAAACCGTAAAATTTTCATAAATAGCGAAAACCCGCGAGGTcgc                                                                            <  1:9138485/150‑1 (MQ=255)
                                                                            cGACAGAGGCCCAAAAGCTCGTTTTCAGCACCTGTCGTTTCATTTCTTTTCTGGGGTTATTTTAAATAAAAACATTAAGTTACGACTAAGAAGAACGGAAATGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTcgc                                                                            <  1:7444878/150‑1 (MQ=255)
                                                                              aCAGAGGCCCAAAAGCTCGTTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGGGGGTATTTTAAATAAAAACAATAAGTTACGACGAAGAAGAACCGAAATGCCTTAAACCGGAAAAATTTAATAAATAGCGAAAACCCGCGAGGTcgccg                                                                          >  2:251166/1‑150 (MQ=255)
                                                                              aCAGAGGCCCAAAAGCTCGTTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGGGGGTATTTTAAATAAAAAAATTAAGTTACGACGAAGAAGAACGGAAAAGCCGTAAACCGGAAAATTTTAATAAATAGCGAAAACCCGCGAGGTcgccc                                                                          >  2:9327807/1‑149 (MQ=255)
                                                                                 gagGCACAAAAGCTCGTTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGGGGGTATTTTAAATAAAAACATTAAGTTACGACGAAGAAGAACGGAAATGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGccc                                                                       <  2:10255608/150‑1 (MQ=255)
                                                                                  agGCCCAAAAGCTCGTTTTCAGCACCTGTCGTTTGCTTTCTTTTCAGGGGGTATTTTAAATAAAAAAATTAATTTACGACGAAGAAGAACGGAAATGCCTTAAACCGGAAAATTTTAATAAATATCGAAAAACCGCGAGGTCGCCGcccc                                                                      >  2:2883785/1‑150 (MQ=14)
                                                                                  agGCCCAAAAGCTCGTTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGGGGGTATTTTAAATAAAAACATTAAGTTACGACGAAGAAGAACGGAAATGCCTTAAACCGGAAAATTTTCATAAATAGCCAAAACCCGCGACGTCGCCGcccc                                                                      >  2:10064656/1‑150 (MQ=255)
                                                                                  agGCCCAAAAGCTCGTTTTCAGCACCTGTCGTTTCATTTCTTTTCAGGGGTTATTTTAAATAAAAACATTAAGTTACGACGAAGAAGAACGGAAATGCCTTAAACCGTAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGcccc                                                                      <  1:10312344/150‑1 (MQ=255)
                                                                                       cAAAAGCTCGTTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGGGGGTATTTTAAATAAAAACATTAAGTTACGACGAAGAAGAACGGAAATGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAAc                                                                 <  1:7279340/150‑1 (MQ=40)
                                                                                       cAAAAGCTCGTTTTCAGCACCTGTCGTTTCCTTTATTTTCAGGGGGTATTTTAAATAAAAACAATAAGTTAAGACGAAGAAGAACCGAAATGCCTTAAAACGGAAAATTTTAATAAATAGCGAAAAACCCCGAGGTCGCCGCCCCGTAAc                                                                 >  2:1052273/1‑150 (MQ=14)
                                                                                            gCTCGTTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGGGGTTATTTTAAATAAAAACATTAAGTTACGACGAAGAAGAACGGAAATGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTc                                                            <  1:9673757/150‑1 (MQ=255)
                                                                                             cTCGTTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGGGGGTATTTTAAATAAAAACATTAAGTTACGACGAAGAAGAACGGAAATGCCTTAAACCGGAAAATTTTCATACATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCg                                                           <  2:5642543/150‑1 (MQ=255)
                                                                                                gTTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGGGGGTATTTTAAATAAAAACATTAAGTTACGACGAAGAAGAACGGAAATGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGAt                                                        <  1:10503084/150‑1 (MQ=38)
                                                                                                 ttttCAGCACCTGTCGTTTCCTTTCTTTTCAGGGGGTATTTTAAATAAAAACATTAAGTTACGACGAAGAAGAACGGAAATGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATc                                                       <  1:5580880/150‑1 (MQ=38)
                                                                                                  tttCAGCACCTGTCGTTTCCTTTCTTTTCAGGGGGTATTTTAAATAAAAACATTAAGTTACGACGAAGAAGAACGGAAATGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCa                                                      <  1:1097795/150‑1 (MQ=38)
                                                                                                   ttCAGCACCTGTCGTTTCCTTTCTTTTGAGGGGGTATTTTAAATAAAAAAAATAATTTAAGACGAATAAGAACGGAAAAGCCTTAAAACGGAAAATTTTAATAAATATAGAAAACCCGCGAGGTAGCCGCCCCGTAAACTGTCGGATCAc                                                     >  2:8379491/1‑150 (MQ=11)
                                                                                                   ttCAGCACCTGTCGTTTCCTTTCTTTTCAGGGGGTATTTTAAATAAAAACATTAAGTTACGACGAAGAAGAACGGAAATGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCAc                                                     <  1:5730195/150‑1 (MQ=38)
                                                                                                        cACCTGTCGTTTGCTTTCTTTTAAGGGGGTATTTTAAATAAAAAAAATAAAATAACACCAAGAAGAAACGAAAAAACATAAAACCGAAAAATTTAAAAAAAATCGAAAACCCCCCAGGTCGCCGCCCCCAAACCAGTCGGATAGCCGGaa                                                >  2:4982276/1‑150 (MQ=255)
                                                                                                        cACCTGTCGTTTCCTTTCTTTTCAGGGGGTATTTTAAATAAAAACATTAAGTTACGACGAAGAAGAACGGAAATGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGaa                                                <  2:4520326/150‑1 (MQ=39)
                                                                                                          ccTGTCGTTTCCTTTCTTTTCAGGGGGTATTTTAAATAAAAACATTAAGTTACGACGAAGAAGAACGGAAATGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAg                                              <  1:3618179/150‑1 (MQ=39)
                                                                                                             gTCGTTTCCTTTCTTTTCAGGGGGTATTTTAAATAAAAACATTAAGTTACGACGAAGAAGAACGGAAATGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGAc                                           <  2:3550134/150‑1 (MQ=39)
                                                                                                             gTCGTTTCCTTTCTTTTCAGGGGGTATTTTAAATAAAAACATTAAGTTACGACGAAGAAGAACGGAAATGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGAc                                           <  2:1902991/150‑1 (MQ=39)
                                                                                                                     ctttctttTCAGGGGGTATTTTAAATAAAAACATTAAGTTACGACGAAGAAGAACGGAAATGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAg                                   <  2:2746259/150‑1 (MQ=255)
                                                                                                                      tttctttTCAGGGGGTATTTTAAATAAAAACATTAAGTTACGACGAAGAAGAACGGAAATGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGt                                  <  1:8659022/150‑1 (MQ=255)
                                                                                                                      tttctttTCAGGGGGTATTTTAAATAAAAACATTAAGTTACGACGAAGAAGAACGGAAATGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGt                                  >  2:4395148/1‑150 (MQ=255)
                                                                                                                      tttctttTCAGGGGGTATTTTAAATAAAAACATTAAGTTACGACGAAGAAGAACGGAAATGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGt                                  >  1:10392228/1‑150 (MQ=255)
                                                                                                                      tttctttTCAGGGGGTATTTTAAATAAAAACATTAAGTTACGACGAAGAAGAACGGAAATGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGt                                  <  2:7808073/150‑1 (MQ=255)
                                                                                                                      tttctttTCAGGGGGTATTTTAAATAAAAACATTAAGTTACGACGAAGAAGAACGGAAATGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGt                                  >  1:6423587/1‑150 (MQ=255)
                                                                                                                         ctttTCAGGGGTTATTTTAAATAAAAACATTAAGTTACGACGAATAAGAACGGAAATGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGAt                               <  2:9694988/150‑1 (MQ=255)
                                                                                                                          ttttCAGGGGGTATTTTAAATAAAAACATTAAGTTACGACGAAGAAGAACGGAAATGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATa                              >  1:5377609/1‑150 (MQ=255)
                                                                                                                          ttttCAGGGGGTACTTTAAATAACAACATTCAGGTACGACGAAGAAGAACGGAAATGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAACGTGATa                              <  1:3349604/150‑1 (MQ=14)
                                                                                                                           tttCAGGGGGTATTTTAAATAAAAACATTAAGTTACGACGAAGAAGAACGGAAATGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATaa                             <  2:6157138/150‑1 (MQ=255)
                                                                                                                           tttCAGGGGGTATTTTAAATAAAAACATTAAGTTACGACGAAGAAGAACGGAAATGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATaa                             >  1:3951178/1‑150 (MQ=255)
                                                                                                                             tCAGGGGGTATTTTAAATAAAAACATTAAGTTACGACGAAGAAGAACGGAAATGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATg                           <  2:8741048/150‑1 (MQ=255)
                                                                                                                               aGGGGGTATTTTAAATAAAAACATTAAGTTACGACGAAGAAGAACGGAAATGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGAt                         <  2:3380052/150‑1 (MQ=255)
                                                                                                                                  gggTATTTTAAATAAAAACATTAAGTTACGACGAAGAAGAACGGAAATGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTat                      <  1:7153852/150‑1 (MQ=255)
                                                                                                                                   ggTATTTTAAATAAAAACATTAAGTTACGACGAAGAAGAACGGAAATGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTatc                     >  1:679144/1‑150 (MQ=255)
                                                                                                                                       ttttAAATAAAAACATTAAGTTACGACGAAGAAGAACGGAAATGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCt                 >  1:2439411/1‑150 (MQ=255)
                                                                                                                                        tttAAATAAAAACATTAAGTTACGACGAAGAAGAACGGAAATGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTa                >  2:1590729/1‑150 (MQ=255)
                                                                                                                                         ttAAATAAAAACATTAAGTTACGACGAAGAAGAACGGAAATGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTAc               <  2:5086875/150‑1 (MQ=255)
                                                                                                                                         ttAAATAAAAACATTAAGTTACGACGAAGAAGAACGGAAATGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTAc               <  1:10601656/150‑1 (MQ=255)
                                                                                                                                         ttAAATAAAAACATTAAGTTACGACGAAGAAGAACGGAAATGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTAc               <  1:5956189/150‑1 (MQ=255)
                                                                                                                                         ttAAATAAAAACATTAAGTTACGACGAAGAAGAACGGAAATGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACACGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTAc               >  1:7946975/1‑150 (MQ=255)
                                                                                                                                                aaaaCATTAAGTTACGACGAAGAAGAACGGAAATGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATcac        >  1:2942209/1‑150 (MQ=255)
                                                                                                                                                 aaaCATTAAGTTACGACGAAGAAGAACGGAAATGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATcaca       >  1:9781631/1‑150 (MQ=255)
                                                                                                                                                   aCATTAAGTTACGACGAAGAAGAACGGAAATGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATCACAAc     <  1:1776499/150‑1 (MQ=255)
                                                                                                                                                     aTTAAGTTACGACGAAGAAGAACGGAAATGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATCACAAcgt   >  2:4840248/1‑150 (MQ=255)
                                                                                                                                                     aTTAAGTTACGACGAAGAAGAACGGAAATGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATCACAAcgt   >  2:3202063/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                      
CGCACCGAAAATGTCAGCCAGCTGCTTTTTGTTGACTTCCATTGTTCATTCCACGGCCAAAAACAGAGAAAGGAAACGACAGAGGCCCAAAAGCTCGTTTTCAGCACCTGTCGTTTCCTTTCTTTTCAGGGGGTATTTTAAATAAAAACATTAAGTTACGACGAAGAAGAACGGAAATGCCTTAAACCGGAAAATTTTCATAAATAGCGAAAACCCGCGAGGTCGCCGCCCCGTAACCTGTCGGATCACCGGAAAGGACCCGTAAAGTGATAATGATTATCATCTACATATCACAACGTG  >  NC_000913/1636326‑1636625

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: