Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,720,364 G→T 9.3% intergenic (+21/+26) slyB → / ← slyA outer membrane lipoprotein/global transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,720,3640GT 9.3% 1525.7 / 58.8 480intergenic (+21/+26)slyB/slyAouter membrane lipoprotein/global transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (278/144);  new base T (5/52);  total (283/197)
Fisher's exact test for biased strand distribution p-value = 3.77e-17
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 498 total aligned reads displayed.

CTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGTTAATAACAGCT  >  NC_000913/1720215‑1720513
                                                                                                                                                     |                                                                                                                                                     
cTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAgg                                                                                                                                                       >  1:1016665/1‑150 (MQ=255)
cTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAgg                                                                                                                                                       <  1:8692065/150‑1 (MQ=255)
cTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAgg                                                                                                                                                       >  2:10938697/1‑150 (MQ=255)
cTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAgg                                                                                                                                                       <  1:5504347/150‑1 (MQ=255)
 tGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGAACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGt                                                                                                                                                      >  1:5246332/1‑150 (MQ=255)
   gAAATTCGTAAAGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTa                                                                                                                                                    >  1:4305454/1‑150 (MQ=255)
    aaaTTCGTAAAGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTAc                                                                                                                                                   <  1:3867464/150‑1 (MQ=255)
    aaaTTCGTAAAGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGACAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCTCGCTAAATAAGTTTGCGTGTGGTCAGGTTAc                                                                                                                                                   <  1:1355486/150‑1 (MQ=255)
     aaTTCGTAAAGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACt                                                                                                                                                  <  1:1152438/150‑1 (MQ=255)
      aTTCGTAAAGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTg                                                                                                                                                 <  2:4707350/150‑1 (MQ=255)
      aTTCGTAAAGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTg                                                                                                                                                 <  2:2976613/150‑1 (MQ=255)
       ttCGTAAAGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGa                                                                                                                                                >  1:2046818/1‑150 (MQ=255)
       ttCGTAAAGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGa                                                                                                                                                <  2:101885/150‑1 (MQ=255)
       ttCGTAAAGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGa                                                                                                                                                >  1:10733758/1‑150 (MQ=255)
        tCGTAAAGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGAc                                                                                                                                               <  2:762629/150‑1 (MQ=255)
        tCGTAAAGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCATACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGAc                                                                                                                                               <  2:5142458/150‑1 (MQ=255)
            taaGACGATGGTACTACCATCATGGTGTGACAGAATCAAGTCAAAACTCGTTTCTCTCCGGGCCAACGTGTCGTACTTGCCAGAAATTGCAGTCATGTGACCGTTTCTCCGCTCTAAATAAGTTTTCTTGTTGTAAGTAACCTGACcaca                                                                                                                                           <  2:7008258/149‑1 (MQ=255)
            aaaGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACcaca                                                                                                                                           >  2:9558230/1‑150 (MQ=255)
             aaGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCacac                                                                                                                                          >  2:5989661/1‑150 (MQ=255)
               gACTATGTTAATACCATCTTGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGTTGACCTTTTCTCCGCGCTAAATAAGTTTGGTTGTGGTCAGTTTACTGACCACACGc                                                                                                                                        <  2:5600917/150‑1 (MQ=255)
                   aTGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGccccc                                                                                                                                    >  2:4015073/1‑150 (MQ=255)
                    tGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCt                                                                                                                                   >  2:7465637/1‑150 (MQ=255)
                       tAATACAATTTTGGTTTTAAATAAACATGGAAACACTCGTTTCTCTCGGGGCAATGGTTTCTTAGTGGCAAGAATTGTATTTCTGTTTACCTTTTCTCCGCGATAATTATTTTTGCGTGTGGTCTTTTTACTGACCACACGCCCCCTTCa                                                                                                                                <  2:424178/150‑1 (MQ=255)
                             cttgatGGTGGTACATAAACAAGGCAACACTCGTTTCTCTCCGGGCAAACGTGTCGTACTGGCCAGCAATGGCTGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCAc                                                                                                                          <  2:10031274/146‑1 (MQ=255)
                             catcatGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCAc                                                                                                                          >  1:7744883/1‑150 (MQ=255)
                                cttggtgtTACAGAAACAAGGCAACACTGGTTTCTCTCGGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCCGGTGACCGTTTCTCCGCGATAAATATGTTTGGGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCt                                                                                                                       <  2:540213/148‑1 (MQ=255)
                                  tggtgtTACATAAACATGGCAACAGTCGTTTCTCTCGGGGCAAACGTGTCTTAGTGGCCAGAATTGGCTGTCTGGTGACCGTTTCTCGGCGCTAAATATGTTGGGGTGTGGTCTGGTTAGTGACCACACGCCCCCTTCATTTCACCCttt                                                                                                                     <  2:5906910/150‑1 (MQ=255)
                                  tggtggTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCttt                                                                                                                     >  2:10860831/1‑150 (MQ=255)
                                   ggtggtACAGAAACACGGCAACACTCGTTTCTCTCCGGGCCAACGTGTGGTCCTGGCCAGCAATGGCAGTCTGGTGACCTTTTCACCGCGCTAAATATGTTTGGGTTTGTTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTg                                                                                                                    <  2:7997936/150‑1 (MQ=255)
                                     tggtACAGAAACCTGGCAACACTGTTTTCTCTCGGGGCAACGGTGTGGTAGGGGCCTGAATGGGCTGTCTGTTGCCGGTTTCTCGGGGTTAATTATGTTTGGGTGTGTTCTGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGc                                                                                                                  <  2:1371818/150‑1 (MQ=255)
                                     tggtACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCACTGGCAGTCCGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTGCAGTTCACCCGTTGGc                                                                                                                  >  1:1036005/1‑150 (MQ=255)
                                        tACAGAAACATGGCAACACTCGTTTCTGGCGGGGCCATGTTGTCGTAGGGGCATGAAATGGATGTCTGTTGCCGTTTTCTCGGCGCTAAATATTTTTGCTTGTGGTCTGTTTCCTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTg                                                                                                               <  2:2238044/150‑1 (MQ=255)
                                          cAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTa                                                                                                             >  2:4472342/1‑150 (MQ=255)
                                          cAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTa                                                                                                             >  2:1068363/1‑150 (MQ=255)
                                           ataaaCAAGCCAACACTCTTTTCTCGCGGGGCCAAGGTTTCTTACGGCCCAGATTTGGCTTTCTGGTGCCGTTTTCTCCGCTATAAATATTTTTGGTTGTGTTCTTTTTACTCACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTaa                                                                                                            <  2:6518268/148‑1 (MQ=255)
                                           aGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTaa                                                                                                            >  1:1898157/1‑150 (MQ=255)
                                                 aaGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCATCATTTCACCCTTTGGCCTGTAACTCAAt                                                                                                      >  1:8491700/1‑150 (MQ=255)
                                                    gCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGat                                                                                                   >  1:8064555/1‑150 (MQ=255)
                                                    gCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGat                                                                                                   >  2:3654829/1‑150 (MQ=255)
                                                        cacTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGAtatta                                                                                               >  2:4796636/1‑150 (MQ=255)
                                                           tCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGc                                                                                            >  1:8854180/1‑150 (MQ=255)
                                                              tttCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCa                                                                                         >  2:3493158/1‑150 (MQ=255)
                                                              tttCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCa                                                                                         >  2:9275304/1‑150 (MQ=255)
                                                                 ctctcCGGGCCAACGGGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCCTGTCACCCTTTGGCCTGTAACTCACTGATATTATGCTCAAgg                                                                                      >  1:665455/1‑149 (MQ=255)
                                                                  tctcCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGtt                                                                                     >  1:9730110/1‑150 (MQ=255)
                                                                    tcCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGtttt                                                                                   >  1:9815352/1‑150 (MQ=255)
                                                                     ccGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGCGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTg                                                                                  <  2:7310240/150‑1 (MQ=255)
                                                                      cGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTGTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGc                                                                                 >  2:9322318/1‑150 (MQ=255)
                                                                       gggCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCg                                                                                >  2:3729803/1‑150 (MQ=255)
                                                                                 gtCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTaa                                                                      >  1:7025198/1‑150 (MQ=255)
                                                                                    gTACTGGCCAGCAATGGCAGTCAGGTGACAGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTTCGATGAGAGTAATCa                                                                   >  1:5135719/1‑150 (MQ=255)
                                                                                     tACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTCCCTCACTGATATTATGCTCAAGGTTTGCGATGAGCGTAATCAg                                                                  >  1:5905848/1‑150 (MQ=255)
                                                                                     tACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAg                                                                  >  1:9403801/1‑150 (MQ=255)
                                                                                       cgggCCAGCATGGGCAGTCTGGTGACGGTTTCGCGGCGCAAATTTTGTTTGGGTGTGTTCTGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGtt                                                                <  2:8655935/148‑1 (MQ=255)
                                                                                       cgggCCAGCAAGGGCAGTCCGGTGACCGTTTCGCCGCGCTAAATAAGTTTGCGTGGGGTCAGGTTCCTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGtt                                                                <  2:8914471/148‑1 (MQ=255)
                                                                                        tGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTg                                                               >  2:1453616/1‑150 (MQ=255)
                                                                                          gCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCt                                                             >  2:1945235/1‑150 (MQ=255)
                                                                                          gCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCt                                                             >  2:4970703/1‑150 (MQ=255)
                                                                                          gCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCt                                                             >  2:1244335/1‑150 (MQ=255)
                                                                                          gCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCt                                                             >  2:3844013/1‑150 (MQ=255)
                                                                                           cctgCAATGGCAGACCGTGGACCGTTTCTCCGCGCTAAATTAGTTTGCGTGTGGTCTGGTTACTGACCACACGACCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTc                                                            <  2:6244148/147‑1 (MQ=255)
                                                                                           ccAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTc                                                            <  2:9113213/150‑1 (MQ=255)
                                                                                            cAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCacac                                                                                                                                          <  2:3971412/71‑1 (MQ=255)
                                                                                            cAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTcg                                                           >  2:8100325/1‑149 (MQ=255)
                                                                                            cAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTcc                                                           >  2:6114233/1‑150 (MQ=255)
                                                                                              gCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAg                                                         >  1:3244687/1‑150 (MQ=255)
                                                                                                 aTGGCTGTCTGGGCACCGTTACTCCGCGCTAAATATGTTTGGGTGGGGTCTGGTTACTCACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTc                                                      <  1:6403726/150‑1 (MQ=255)
                                                                                                      aGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTg                                                 >  2:8368198/1‑150 (MQ=255)
                                                                                                       gTCTGGGGACGGTTTCTCCGCGATAAATCAGTTTGCGTGTGGTCCGGTTCCTCACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGc                                                <  2:2466032/150‑1 (MQ=255)
                                                                                                        tccggTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCTGGTTACGGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCg                                               <  2:2663444/147‑1 (MQ=255)
                                                                                                          aGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCGTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGGTGCTCCAGTTCCTCTGCggc                                             >  1:2811821/1‑149 (MQ=255)
                                                                                                              gACCGTTTCTCCGCGCTAAATATGTTTGCGTGGGGTCAGGTTACTCACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATg                                         <  1:6096329/150‑1 (MQ=255)
                                                                                                               aCCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGc                                        >  1:6883424/1‑150 (MQ=255)
                                                                                                                 cGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCACGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGCTGccg                                      >  2:1613645/1‑149 (MQ=255)
                                                                                                                  gTTTCTCCGCGCTAAATACGTTTGGGTGTGGTCTGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCAt                                     <  2:8532053/150‑1 (MQ=255)
                                                                                                                         cgcgCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAatat                              >  2:9489783/1‑150 (MQ=255)
                                                                                                                         cgcgCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAatat                              >  2:5824063/1‑150 (MQ=255)
                                                                                                                           cgcTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCGTCATTTCACCCGTTGGCCTGTAACTCAAGGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCAGGTAATAttt                            >  1:9122462/1‑150 (MQ=255)
                                                                                                                            gcTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTAAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGGTACTCTGCGGAGGTGCCAGGTGATAtttg                           >  1:5629668/1‑149 (MQ=255)
                                                                                                                            gcTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACACAATGATATTATTCTCAAGTTTTTCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTc                           >  1:10563968/1‑150 (MQ=255)
                                                                                                                                       ttGGGTGTGTTCAGTTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGttt                <  1:4137326/150‑1 (MQ=255)
                                                                                                                                        tGCGTGTGGTCTGGTAACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGtttt               <  1:1087301/150‑1 (MQ=255)
                                                                                                                                        tGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGtttt               >  2:6755570/1‑150 (MQ=255)
                                                                                                                                        tGCGTGTGGTCAGGTTACGGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGtttt               <  2:7191057/150‑1 (MQ=255)
                                                                                                                                         gCGTGTGGTCCGGTTCCGCACCCCACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTg              <  1:496159/150‑1 (MQ=255)
                                                                                                                                          ggggtgGTCAGGTTACGGACCCCACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGt             <  2:5292421/147‑1 (MQ=255)
                                                                                                                                          cGTGTGGTCTGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGt             <  1:4645891/150‑1 (MQ=255)
                                                                                                                                           gtgtTGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGtt            <  2:7785466/150‑1 (MQ=255)
                                                                                                                                           gtgtGGTCTGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGtt            <  1:1728991/150‑1 (MQ=255)
                                                                                                                                           gtgtGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGtt            <  1:2439476/150‑1 (MQ=255)
                                                                                                                                           gtgtGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTCCGGCGATGCCATGGACAATTTACGCGCGGGTTTTGtt            >  2:6369401/1‑150 (MQ=255)
                                                                                                                                             gtgGTCTGTTTAGTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGTtaa          <  2:2048462/150‑1 (MQ=255)
                                                                                                                                                gTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGTtaataa       >  2:2166343/1‑150 (MQ=255)
                                                                                                                                                gTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGTtaataa       <  1:8254875/150‑1 (MQ=255)
                                                                                                                                                gTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCAGAGCGGGTTTTGTtaataa       >  2:10480761/1‑150 (MQ=255)
                                                                                                                                                gTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCAGGTAATAGTTCCGCGCGGGTTTTGTtaataa       >  2:6630197/1‑150 (MQ=255)
                                                                                                                                                 tCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGTTAATAAc      >  2:338641/1‑150 (MQ=255)
                                                                                                                                                 tCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCAGGTAATATTTCCGCGCGGGTTTTGTTAATAAc      >  1:2979918/1‑150 (MQ=255)
                                                                                                                                                     gTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCa                                                          <  1:3474708/94‑1 (MQ=255)
                                                                                                                                                     gTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGTTAATAACAGCt  >  2:10414516/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGTTTCTCCGCGCTAAATAAGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGTTAATAACAGCT  >  NC_000913/1720215‑1720513

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: