| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 164,569 | C→G | 21.9% | intergenic (+35/‑161) | hrpB → / → mrcB | putative ATP‑dependent helicase/fused glycosyl transferase and transpeptidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 164,569 | 0 | C | G | 21.9% | 638.3 / 63.0 | 268 | intergenic (+35/‑161) | hrpB/mrcB | putative ATP‑dependent helicase/fused glycosyl transferase and transpeptidase |
| Reads supporting (aligned to +/- strand): ref base C (110/94); new base G (4/58); total (115/153) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.69e-12 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 272 total aligned reads displayed.
GAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTT‑TTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGC‑ACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGC > NC_000913/164420‑164718 | gAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAATGTATTCGTAAGGTTGGTT‑TTCTCCCTCTCCCTGTGGGAGAGGGcc > 1:147290/1‑150 (MQ=255) aaaGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTT‑TTCTCCCTCTCCCTGTGGGAGAGGGCCg > 2:3057059/1‑150 (MQ=255) ggAGCGTACCGTGAGGTGCAAAACGAGATGAACGGGCGTTATCCCACCCAGGTCTGGCCGGACGACCCGGCAAATACGGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTT‑TTCTCCCTCTCCCTGTGGGAGAGGGCCgggg < 2:9633433/150‑1 (MQ=255) gAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTT‑TTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGt > 1:9451287/1‑150 (MQ=255) gAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTT‑TTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGt > 2:10744086/1‑150 (MQ=255) gCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTT‑TTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGa < 2:412684/150‑1 (MQ=255) cGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAACCATGTCTGGCCGGACGATCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTT‑TTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAg < 2:454241/150‑1 (MQ=255) cGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTT‑TTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAg > 2:8003841/1‑150 (MQ=255) gTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTT‑TTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAgg > 1:10040554/1‑150 (MQ=255) tACCGTGCGGTGCAAATCGAGATGAACGGGCGTTATCCCAACCAGGTCTGGCCGGACGCCCCGGCAAATACTGCACCGACGCGCCGGACGAAAACGTTTTCGTACGGTTGGTT‑TTCGCCCGCTCCCGGTGGGAGAGGGCCGGGGTGAggg < 2:966417/150‑1 (MQ=255) ccGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTT‑TTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCa < 2:51811/150‑1 (MQ=255) cGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTT‑TTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGcct > 1:5489630/1‑148 (MQ=255) cGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGATT‑TTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCAt > 2:3240074/1‑150 (MQ=255) ggTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTT‑TTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAgcg > 1:1018042/1‑150 (MQ=255) gTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTT‑TTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAgcgc > 1:7764284/1‑150 (MQ=255) tGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTT‑TTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAgcgcg > 2:9434793/1‑150 (MQ=255) gCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTT‑TTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCCgcgcgc > 1:5380533/1‑150 (MQ=255) aaaaaGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTT‑TTCTCCCTCTCCCTGTAGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCAc < 2:2397362/150‑1 (MQ=255) aaaGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTT‑TTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGt > 1:2964139/1‑150 (MQ=255) aaGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTT‑TTCTCCCTCTCCCTGTGGGGGAGGGCCGGGGTGAGGGCATCAGCGCGCACGtg > 2:743108/1‑149 (MQ=255) tattaatgggCTTTAACCAAAACCTGTCGGGCGGTACGACCCGGAAATTACTGCCCCGACGCGAGTTAATAAAATTTTTTCTTATGGTTGTTT‑TTCCCCCTCCCCCTGTGGTTTGGGGCGGGGTTGCGGGCATCAGCGCGCACGTtcacc < 2:3142937/143‑1 (MQ=255) gAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTT‑TTCTCCCTCTCCCTGTGGGAGAGGGCCggg < 2:2599767/120‑1 (MQ=255) gAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTT‑TTCTCCCTCTCCCTGTGGGAGAGGGCCggg > 1:2599767/1‑120 (MQ=255) atgggCTTTAACCCAAACTGGTCGGGCCGGACGACCCGGAAAATACGGCACCGACGCTACGGACGAAAATTTATTCGTATGGTTGGTT‑TTCTCCCTCGCCCTGTGGGAGTGGGCGGGGGTGAGGGCATCAGCGCGCACGTtcaccctcac < 2:709183/148‑1 (MQ=25) aaGGGCGTTATCCCAACCTGGTCTGGCCGGACGACCCGTCAAATCCTGCACCGACGCGACGGACGAAAATTTATTCGTATGGTTGGTT‑TTCTCCCTCTCCCTGTGGGTGTGGGCGGGGGTGTGGGCATCAGCGCGCACGTtcaccctcac < 2:617059/150‑1 (MQ=37) tgggCGTTAGCCCAAACATGGCTGGCCGGACGACCCGGCAAATCCTGCACCGACGCGACGGACGAAAATGTTTTCTTATGGTTGGTT‑TTCTCCCTCTCCCTGTGGGTGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTtcaccctcacc < 2:4574145/149‑1 (MQ=37) ttATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTT‑TTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCAGCAGCGCGCACGTGGACCCACACCCCAAcc > 1:6369974/1‑150 (MQ=38) ttATCCCAAACATGTCGGGCCGGACGACCCGGAAAATACGGCACCGACGCGACGGACGAAAAATTATTCGTAAGGTTGGTT‑TTCTCCCTCTCCCTGTGGGAGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAAcc < 2:5380533/150‑1 (MQ=38) ttATCCCAAACAGGTCTGGCCGGACGACCCGGCAAATACGGCCCCGAGGCTAGTGACTAAAATTTTTTCTTATGTTTGTTT‑TTCTCCCTACCCCGGTGGGTGCGGGCCGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAAcc < 2:293729/150‑1 (MQ=255) ttATCCAAACCTTCTCGGGCCGCACGACCCGGCAAATACTGCACCGCCGCGCCGGACTAAAAAGTTTTCGTATGGTTGGTT‑TTCCCCCTCTCCCTGTGGGAGAGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAAcc < 2:5179103/150‑1 (MQ=25) taaccccAAACAGGTGGGGCCGGCCGACCCGGCAAATACTGCACCGCGGCGACGGACTAAAAAGTTTTGGTATGGTTGGTT‑TTCGCCCTCCCCGTGGGGGAGTGGGCGGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAAcc < 2:5616464/146‑1 (MQ=255) atattcaggcgcaaaagcccgattttctgttcttctgttacaagacagaagaagatatctctcaaaatttgtgctcgatcggtcccctctcccttgaGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAAcc > 2:1353786‑M1/98‑150 (MQ=255) cccAACCTTGTGGGGCCGGACGCCCCGGCATTTCCTGCCCCGCCGCGCGGGACTAAAATTTTTTCGTATGTTTGGTT‑TTCTCCCTCTCCGGGGGGGTGTGGGGGGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCctct < 2:6678617/150‑1 (MQ=255) cccAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTT‑TTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTGGCCCACCCCCAAAccaaca > 1:9552340/1‑146 (MQ=38) ccAATCTTGGCTGGCCGGACCACCGGGAAATTCCGGCCCCTCCGCGCCGGACGAAAATGTATTCTTTGGGTTGGTT‑TTCGCCCTCTCCCTGGGGGTGTGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCtctc < 1:9849500/150‑1 (MQ=255) ccAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTT‑TTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTGACCCGCACCCAAAcccacac > 1:8093122/1‑146 (MQ=255) ccAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTT‑TTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGCCCCCCCCCCCCAAccccccc > 1:2089773/1‑146 (MQ=38) cAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTT‑TTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGGTCACCCACCCCCAAAcccacaac > 1:6112602/1‑145 (MQ=38) ttGTCTGGCGGCACGACCCGCAAAATACTCCACCGACGCGACGCACTAAAATTTATTCGTATGTTTGTTT‑TTCTCCCTCCCCCTGTGGGAGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCaa < 2:5030470/149‑1 (MQ=25) aTGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTT‑TTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCaa < 2:2684807/150‑1 (MQ=255) gTCTGTCCGGCCGACCCGGCAAATACTGCACCGCCGCGACGGACGAAAAAGTATTCGTACGGTTGGTT‑TTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAgg < 2:7296340/150‑1 (MQ=38) gTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTT‑TTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAAACCTCTCCCTCAAgg > 2:1744447/1‑150 (MQ=255) tGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTT‑TTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAg < 2:10289832/100‑1 (MQ=255) tGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTT‑TTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCACACCCGAACCCACACCCACAAGGgag > 1:9825867/1‑150 (MQ=38) ggCCGGACCACCCGGCAAATACTGCACCTACGCGACGGACTAAAATGTTTTCGTATGGTTGTTT‑TTCTCCAACGCCCTGTGGGTGAGGGCCGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGgaga < 2:573528/150‑1 (MQ=255) gCCGGACGCCCCGCAAAATCCGCCCCCTACGCTCCGGACTAAATATTATGCGTTTGGTTGTTT‑TTCTCCCGCTCCGTGTGGGTGTGGGCGGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGagag < 1:2387064/150‑1 (MQ=255) ccGGACTCCCCGCAAATTACTCCCCCGCCGCGCCGTACGAAAATTTA‑TAGTTATGTTGGTTGTCCCCCCTCCCCCTGGGGGTGTGGGCGGGGGTGTGGCCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTTGAGGGAGAgg < 1:6808108/150‑1 (MQ=255) cGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTT‑TTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGGTCACCCGCACCCACACCCACACCCCCCAGGGAGAggg > 1:4366184/1‑150 (MQ=37) gacgacCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTT‑TTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCACACCCTAACCCACTCCCACAAGGGGGAggggg > 2:3662965/1‑149 (MQ=255) gacgacCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTT‑TTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGCACCCGCACCCAAACCCACAACCACAAGGGAggggggg > 1:1841835/1‑149 (MQ=37) acgacCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTT‑TTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCAGCAGCGCGCACGTGGGCCCGCACCCCAACCCACACCCACAAGGGGGAgggggg > 1:10818156/1‑148 (MQ=37) gacCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTT‑TTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTGGCCCGCACCCAAAACCACAACCCCAAGCGGGAGGGGCCCg > 1:8476966/1‑150 (MQ=25) gacCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTT‑TTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGACCCCCCCCCGAAACCCCAACCCCAAGGGGGTGGGGGCCg > 1:1865563/1‑150 (MQ=25) ccGGCAAATACGGCACCGACGCGACGGACTAAAATGTATTCGTAAGGTTGGTT‑TTCTCCCTCGCCCTGTGGGTGAGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATc < 2:8660648/150‑1 (MQ=38) gCAAATCCGGCCCCGACGCGCCGGACGAAAAAGTATTCGTACGGTTGGTT‑TTCTCCCCCCCCCGGGGGGAGAGGGCCGGGGTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAg < 2:5811129/150‑1 (MQ=255) gCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTT‑TTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCACACCCGCACCCACGCCCGCCCGGGAGAGGGGGCCGATCGAg > 2:9814813/1‑150 (MQ=37) cAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTT‑TTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGc < 2:10042544/150‑1 (MQ=255) tGCCCCGACGCGACGGACGAAAAATTTTTCTTATGGTTGGTT‑TTCTCCCTCCCCCTGTGGGAGAGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGC‑ACAAAtt < 2:1539184/150‑1 (MQ=38) gCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTT‑TTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGCGCCCACACCCACACCCGCTCCCGCCCGGGAGAGGGGGCCGAGCGCGC‑CCAAAatt > 2:10851448/1‑150 (MQ=25) cacccccgcgacgGACGAAAATTTATCCTCATGGTTTTTT‑AACCCCCTAGCCCTGTGTTGGAGGGCCGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGC‑ACAAAtttt < 1:4045932/144‑1 (MQ=255) ccgccgCGCGGGACTAAAATTTTTTCGTATGGTGGGTT‑TTCTCCCTCTCCGTGTGGGTGTGGGCGGGGGTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGC‑ACAAATTTTga < 1:7252915/150‑1 (MQ=255) ccgccgCGACGGACTAAAATGTTTTCGTATGTTTGTTT‑TTCTCCCTCTCCCTGTGGGAGTGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGC‑ACAAATTTTga < 2:8476966/150‑1 (MQ=38) cccacgcgacgCACCAAAAAGTATTCGAAAGGTTGGTT‑TACACCCACCCCCTGTGGCAGAGGGCCGGGGTGAGGCAATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGC‑ACAAATTTTga < 2:8796251/147‑1 (MQ=255) ccGACGCGACGTACGAAAAATTTTTCTTATGTTTGTTT‑TTCTCCCTCTCCGTGTGGGTGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGC‑ACAAATTTTga < 1:2683272/150‑1 (MQ=38) gcggcgacgTACTAAAAAGTTTTCTTATGGTTGTTT‑TCCGCCCTCCCCCGGTGGGTGTGGGCGGGGGTGTGGGCATCAGCGCGCACGGTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGC‑ACAAATTTTgaga < 1:6852933/147‑1 (MQ=255) acgcgacgGACGAAAAAGTATTCGTAAGGTTGGTT‑TTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCACACCCAAAACCACAACCACAAGGGAGAGTGGACCCAACGAGC‑ACAAAATTTtagag > 2:2789576/1‑150 (MQ=25) gagggaccAAAATTTATTCTTACGTTTGGTT‑TTCCCCCACCCCGGGTGGGTGGGGGCCGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGC‑ACAAATTTTGAGAGatat < 2:1972041/147‑1 (MQ=255) acgAAAAAGTATTCGTAAGGTTGGTT‑TTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGCCCCCCACCCAAAAACACACCCACAAGGGGGCGGGGCCCGATCGAGC‑CCAAATTTTTAGATATAtcatat > 1:9941741/1‑146 (MQ=21) cgAAAAAGTATTCTTACGGTTGGTT‑TCCCCCCTCTCCCTGTGGCAGAGGCCGGGGGTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGC‑ACAAATTTTGAGAGATAtcttcta < 1:5734634/150‑2 (MQ=255) aaaaaTTATTCTAATGGTTGGTT‑ATCACCCTCCCCCTGTGGGAGTGGCCCGGGGTGAGCCCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCACCCTCAAGGGAGAGGGGACCGATCGAGC‑ACAAATTTTGAGAGATAtcttcttct < 1:5063565/150‑1 (MQ=255) aaaaGTAATCGTAAGGTTGGTT‑TTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCCACCACCCCCAAAACCACTCCCACAAGGGCGACGGGCCCCATCCAGCAACAAATTTT‑CGAGATAtcttcttcgg > 2:6043175/1‑148 (MQ=16) aaGTATTCGTATGGTTGGTT‑TTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGC‑ACAAATTTTGAGAGATATCTTCTTCTGTc < 1:7958573/150‑1 (MQ=255) gTATTCGTAAGGTTGTTT‑TTCTCCCACTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGC‑ACAAATTTTGAGAGATATCTTCTTCTGTCtt < 2:3618661/150‑1 (MQ=255) tATTCTTATGGTTGTTT‑TTCTCCCTCTCCCGGGGGGAGTGGGCGGGGGTGGGGGCCTCAGCGCGCACGTTCACCCTCACCCTACCCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGC‑ACAAAGTTTGAGAGAGATCTTCTTCTGTCTTg < 2:4908414/150‑1 (MQ=255) aTTCGTATGGTTGGTT‑TTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGC‑ACAAATTTTGAGAGATATCTTCTTCTGTCTTGt < 1:7361234/150‑1 (MQ=255) ttCGTATGGTTGGTT‑TTCTCCCTCTCCCTGTGGTAGTGGGCGGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGC‑ACAAATTTTGAGAGATATCTTCTTCTGTCTTGTa < 1:5805344/150‑1 (MQ=255) tcttatggttggtt‑TTCTCCCTCTCCCTGTGGGTGAGGGCGGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGC‑ACAAATTTTGAGAGATATCTTCTTCTGTCTTGTaa < 2:3501642/144‑1 (MQ=39) tCGTAAGGTTGGTT‑TTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGGTCACCCTCAACCGAACCCACTCCCACAAGGGAGAGGGGACCGATCGAGC‑ACAAAATTTGAGTGATATCTTATTCTGTGTTGTaa > 1:1001509/1‑150 (MQ=37) gtttggttgttt‑TTCTCCCTCTCCCTGGGGGTGTGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGC‑ACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACa < 2:683384/146‑1 (MQ=39) gTATGGTTGGTT‑TTCGCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGC‑ACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACa < 1:10740866/150‑1 (MQ=255) cggttggtt‑TACTCCCTCCCCCTGGGGGAGTGGGCCGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGC‑ACAAATTTTGAGAGTTATCTTCTTCTGTCTTGTAACagaa < 1:921224/149‑1 (MQ=255) gttggtc‑TTCCCCCCCCCCCTGGGGGTGGGGGCCGGGGGGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGC‑ACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACagaaga < 1:3201849/150‑1 (MQ=255) tt‑ttCTCCCTCTCCCTGTGGGAGAGGGCCGGGGGGAGGGCCTCAGCGCGCACGGTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGC‑ACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGa < 2:5898068/150‑1 (MQ=255) ttgtcgccctctccctGTGGGAGTGGGCGGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGC‑ACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGa < 1:1213530/144‑1 (MQ=255) ccccccccctGGGGGAGAGGGCCGGGGTGAGGGCATCAGCGAGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGC‑ACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCg < 1:1031213/144‑1 (MQ=255) cctctccctGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGC‑ACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCgg < 1:3667211/150‑1 (MQ=255) ctctccctGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGGTCACCCTCACCCGAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGC‑ACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCggg < 2:9049874/150‑1 (MQ=255) tccctGTGGGAGAGGGCCGGGGTGAGGGCATCCGCGCGCACGTGGACCCGCACCCAAACCCCCGCCCACCAGGGCGGGGGGGCCCCGCGCGC‑CCAAAGTGTTGGTGGTATAAAGATCTGGGGTGTAACAGAAGAACACGAAATCgggggg > 2:7910296/1‑147 (MQ=255) ctGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGCACCCACACCCGAACCCACACCCTCAAGGGAGAGGGGACCCAACGAGC‑ACAAATTTTGAGAGATATATTCTTCTGTCGTTTAACAGAAGAAAAGAAAATCGGGCGTTTg > 2:9101082/1‑150 (MQ=255) tgtgGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGGGGGGACCGATCGAGC‑ACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTgc > 2:7051134/1‑150 (MQ=255) ggggtgtgggCGGGGTTGTGGGCATCAGCGCGCACGTTCACACTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGC‑ACAAATTTTGAGAGCTATCTTCTTCTGTCTTGTAACAGAAGAACAGAATATCGGGCTTTTgcgc < 2:10099539/143‑1 (MQ=255) ggggcgcgggCGGGGGTGCGGCCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGC‑ACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTgcgc < 1:4665968/143‑1 (MQ=255) gGGGAGTGGGCGGGGGTGAGGCCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGC‑ACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTgcgc > 2:2304225‑M1/2‑150 (MQ=255) gtcgtGGGCGGGGGGGCGGCCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGC‑ACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCt > 1:10563457‑M1/6‑150 (MQ=255) ggtgagGGCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGC‑ACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCt < 1:6459175/147‑1 (MQ=255) GAGAGGGCCGGGGGGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGC‑ACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTg > 1:10392241‑M1/1‑150 (MQ=255) GAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCGCACCCCAACCCACTCCCACAAGGGGGAGGGGACCCATCGAGC‑ACAAAATTTGAGTGATAAATTATTATGTCTTGTAACAGAAGAACAGAAAATAGGGCTTTTGCGCCGGaa < 2:8203458‑M1/150‑1 (MQ=255) ggCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCACCCCCAAAACCACACCCGCAATGGGGAGGGGGCCGATCGAGC‑CCAAAATTTGAGATATATATTATTATGTCTTTTAAAAGAAGAAAAGAAAATACGGCTTTTTCGCCAGAaaat > 2:6401717/1‑150 (MQ=255) ggCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCACACCCAAACCCACTACCACCAGGGAGAGGGGACCGATCGAGC‑ACAAATTTTTAGAGTTATAATATTAAGTAATTTAACAAAAGACACCAAAAACGGGCTTTTGAGCCGGAatat > 2:8221209/1‑150 (MQ=255) GCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCtctc < 2:2441762‑M1/50‑1 (MQ=255) | GAAAGGAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTT‑TTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGC‑ACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGC > NC_000913/164420‑164718 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |