Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,177,229 G→T 6.5% intergenic (‑25/+283) gatY ← / ← fbaB D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,177,2290GT 6.5% 1656.2 / 24.4 551intergenic (‑25/+283)gatY/fbaBD‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
Reads supporting (aligned to +/- strand):  ref base G (280/213);  new base T (6/50);  total (287/264)
Fisher's exact test for biased strand distribution p-value = 1.38e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 558 total aligned reads displayed.

GCAGGTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGA  >  NC_000913/2177081‑2177378
                                                                                                                                                    |                                                                                                                                                       
    gTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTc                                                                                                                                                    <  2:3502506/150‑1 (MQ=255)
       ggCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCaaa                                                                                                                                                 <  1:2422549/150‑1 (MQ=255)
        gCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCaaaa                                                                                                                                                >  1:1702626/1‑150 (MQ=255)
        gCAGCGGTTTCTACCACCACATGCATCGTTTCGAGATTGTGAATATTTAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTATCATATCCTGTCGTGTGGTTTAGAATTCaaaa                                                                                                                                                >  2:6499477/1‑150 (MQ=255)
         cAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAat                                                                                                                                               >  2:7159646/1‑150 (MQ=255)
          aGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAata                                                                                                                                              >  2:2379762/1‑150 (MQ=255)
           gCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAatat                                                                                                                                             <  1:6253457/150‑1 (MQ=255)
           gCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAatat                                                                                                                                             <  1:3366092/150‑1 (MQ=255)
             ggTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATaa                                                                                                                                           <  2:10739111/150‑1 (MQ=255)
                   tACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAAAATAATGaaaa                                                                                                                                    >  2:6838521/1‑149 (MQ=255)
                       accacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAattatt                                                                                                                                <  1:10934980/150‑1 (MQ=255)
                        ccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTAttt                                                                                                                               <  2:7478272/150‑1 (MQ=255)
                         cacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTg                                                                                                                              >  2:6179443/1‑150 (MQ=255)
                          acTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGt                                                                                                                             <  2:1724864/150‑1 (MQ=255)
                          acTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATATAATGAAAATATTTGt                                                                                                                             >  2:3427184/1‑150 (MQ=255)
                           cTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTAtttttt                                                                                                                            >  1:7697830/1‑150 (MQ=255)
                           cTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGtt                                                                                                                            <  2:4382554/150‑1 (MQ=255)
                               cATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTTTTTCGATTTAAAAAAAAAAAGAAATTATTTTTTTTTa                                                                                                                        >  1:3240716/1‑150 (MQ=255)
                                 tCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTAGATTTCAAAATATAATGAAAATATTTTTTTTTaaa                                                                                                                      >  1:5867968/1‑150 (MQ=255)
                                 tCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTaaa                                                                                                                      >  2:5829410/1‑150 (MQ=255)
                                    tttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAatat                                                                                                                   >  2:10020787/1‑150 (MQ=255)
                                    tttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAAAATAATGAAATTATTTGTTTTTAAatat                                                                                                                   >  2:8565255/1‑150 (MQ=255)
                                       cGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATGTCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCga                                                                                                                <  1:5226887/150‑1 (MQ=255)
                                       cGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCga                                                                                                                <  2:5460775/150‑1 (MQ=255)
                                       cGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATATAATTAAATTATTTTTTTTTTAatatagt                                                                                                                >  1:10378734/1‑147 (MQ=255)
                                        gagaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAAATATTTGTTTTTAAATATagag                                                                                                               >  2:9580570/1‑150 (MQ=255)
                                         agaTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCgaga                                                                                                              <  1:901394/150‑1 (MQ=255)
                                           aTTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATATAATGAAATTATTTTTTTTTAAATATAGAGATa                                                                                                            >  1:5553691/1‑150 (MQ=255)
                                             tgtgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAAc                                                                                                          <  2:2181425/150‑1 (MQ=255)
                                               tgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGa                                                                                                        >  2:10066473/1‑150 (MQ=255)
                                                gAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTCGATTTCAAAATATAATTAAAATATTTTTTTTTAAATATAGAGATAAcaga                                                                                                       >  1:6668403/1‑147 (MQ=255)
                                                gAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAGTGAAATTATTTGTTTTTAAATATCGAGATAACGAt                                                                                                       <  1:3684979/150‑1 (MQ=255)
                                                   tatTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATcac                                                                                                    <  1:6008211/150‑1 (MQ=255)
                                                    atTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTCAAAATATAATTTAATTATTTTTTTTTAAATATAGAGATAAAGATaaca                                                                                                   >  2:1901335/1‑150 (MQ=255)
                                                    atTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTAGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATcaca                                                                                                   <  2:10183498/150‑1 (MQ=255)
                                                       gAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACaaaa                                                                                                >  2:1089362/1‑150 (MQ=255)
                                                        aaTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGTTTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACaaaaa                                                                                               <  2:299396/150‑1 (MQ=255)
                                                        aaTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATAAAATGAAATTATTTTTTTTTAAATATAGAGATAAAGATCACaaaaa                                                                                               >  1:2751312/1‑150 (MQ=255)
                                                        aaTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGTTTTGAAAATATAATGAAATTATTTTTTTTTTAATATAGAGATAACGATCACaaaaa                                                                                               >  1:9235558/1‑150 (MQ=255)
                                                          tGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAAATAAATTATTTGTTTTTAAATATAGAGATAAAGATCACAAAAACg                                                                                             >  1:5367178/1‑150 (MQ=255)
                                                          tGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAAAGAAATTATTTGTTTTTAAATATAGAGATAACGATCACaaaaaag                                                                                             >  2:8399819/1‑148 (MQ=255)
                                                            ccGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTATTTATATTTAAAAATAAAAATAAAAAATTTTTTTTTTAAAATACAAATAACGATAACaaaaaaaaa                                                                                           >  1:9743279/1‑146 (MQ=255)
                                                             cGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACa                                                                                          <  2:5983667/150‑1 (MQ=255)
                                                                  ccGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTGGATTTCAAAATATAATTTAATTATTTTTTTTTAAATATCGAGATATAGATAACAAAAAAGACAATATg                                                                                     >  1:3971690/1‑150 (MQ=255)
                                                                    gCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTAGATTTAAAAATATAAAGAAATTATTTTTTTTTAAATATAGAGATAAAGATAAAAAAAAAGACAAAATTaa                                                                                   >  2:2460751/1‑150 (MQ=255)
                                                                    gCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGGTTTGAAAAAATTATGTAAAAATTTTTTTTTAAAAATAGAGATAAACATAACCAAAAAAACAATAAGaa                                                                                   >  1:1001602/1‑150 (MQ=255)
                                                                     cATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATAGAGATAACGATCACAAAAACGACAATATGaaa                                                                                  >  2:9140028/1‑150 (MQ=255)
                                                                     cATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAATATAAATAAAATATTTTTTTTTTAATATAGAGATAAAGATAACAAAAACGACAAAATGaaa                                                                                  >  1:2085589/1‑150 (MQ=255)
                                                                      aTAACCGCGGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATAACACGTACTTTTTCTTATCCTGTCGTTTGTTTTCTATTTCTAATTATAATGAATTTTTTTGTTTTTAATTATCGAGTTAACGATCACAAACTCGACAATATGaaaa                                                                                 <  2:6920030/150‑1 (MQ=255)
                                                                        aaCCGCCGCGCTGTGCGGTGGTCAGCATCTCCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTATAATTTTTAAAAATATAATTAAATTATTTGTTTTTAAAAAGGGAGGGAACCATAACAAAATCGTCGAAATTAAAatt                                                                               >  1:1768606/1‑150 (MQ=255)
                                                                             ccgcGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTGTTCGATTTGAAAAAATAATGAAAATATTTTTTTTTAAATATCGAGATAACGATCCCAAAAACGACAAAATGAAAATACTTCg                                                                          >  1:4355686/1‑150 (MQ=255)
                                                                               gcgcGGTGCGTTGTTCCGCATCCGCTTTGCCGACACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCgag                                                                       <  2:9015181/150‑1 (MQ=255)
                                                                                   tgtgCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGt                                                                   <  2:469241/150‑1 (MQ=255)
                                                                                       cGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGaaa                                                               >  2:1692140/1‑150 (MQ=255)
                                                                                        gttgttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAAAATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTAGAGGAGGGAAAg                                                              >  2:7656709/1‑150 (MQ=255)
                                                                                          tgttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGc                                                            <  2:4637678/150‑1 (MQ=255)
                                                                                            ttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCaa                                                          <  2:8775849/150‑1 (MQ=255)
                                                                                             tCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCaaa                                                         <  2:1397137/150‑1 (MQ=255)
                                                                                              ctgCATCTGCTTTGTCTATACCACGTAAATTTTCATAGCCTGTTTTTTTTTTTCTTTTAAAAAATATAATTATTTTTTTTTTTTTTAAATTTCGTTATAACGAACAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCaaaa                                                        <  1:4197252/148‑1 (MQ=255)
                                                                                              cAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAAAATAATTAAATTATTTGTTTTTAAATATCGAGATAACCATGACAAAAAAGACAATATTAAAATTATTAGAGGAGTGAAAGGCaaaa                                                        >  2:4940199/1‑150 (MQ=255)
                                                                                              cAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGAATTAAAAAAATAAATAAAATATTTTTTTTTAAAAATAGAGATAAAGATCACAAAAAACAAAAAAAGAAAAATAATAGAGGAGTGTAAGGCcaaa                                                        >  1:7482813/1‑150 (MQ=255)
                                                                                                 cATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCaaaaaaa                                                     >  2:1605415/1‑150 (MQ=255)
                                                                                                  ataggCTTTGTCGATACCACGTACATTTTCATATCCGTTCGTTTGTTTTCTATTTCAAAATATAATGAAATTTTTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAAc                                                    <  2:8490711/146‑1 (MQ=255)
                                                                                                  aTCTGCTTTGTCGATACCCCGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAAc                                                    <  2:5118435/150‑1 (MQ=255)
                                                                                                  aTCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAAc                                                    <  2:9768738/150‑1 (MQ=255)
                                                                                                   tCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATATAATGAAATTATTTTTTTTTAAATAAAGAGATAACGATCACAAAAAACACAAAATGAAAATTATTCGAGGTGTGAAAGGCaaaaaaaag                                                   >  2:4843821/1‑148 (MQ=255)
                                                                                                     tGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTCa                                                                                                                                                   >  2:3761947/1‑54 (MQ=255)
                                                                                                       cTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTTTTTGGGTTTCAAAATATAATTGAAATAATTTTTTTTTAATATAGTGGAAAAGATCACAAAAAACACAAAAAGAAAAATATTAGAGGGGGGGAAAGCCaaaaaaaagccc                                                >  1:6894845/1‑145 (MQ=255)
                                                                                                        tttGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTGGAATTCAAAATATAAAGAAATTATTTTTTTTTAAAAATAGAGAAAAAGATAACAAAAAACAAAAAAAGAAAATTATTAGAGGAGGGAAAGTCAAAAAAAAGGccac                                              >  2:4074197/1‑148 (MQ=255)
                                                                                                        tttGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACCATCCCAAAAACGACAATATGAATATGATTCGTGGAGGGAACGGCAAAAAAACGGccgc                                              >  2:316937/1‑148 (MQ=255)
                                                                                                            tctaTACCACGTACATTTTAATATCCTGTCTTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAACACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGa                                          <  2:10402242/147‑1 (MQ=255)
                                                                                                              gATACCACTTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATa                                        <  2:6617406/150‑1 (MQ=255)
                                                                                                               aTACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAg                                       >  2:9919424/1‑150 (MQ=255)
                                                                                                                   cACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACCATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGaa                                   >  2:724679/1‑150 (MQ=255)
                                                                                                                   cACGTAAATTTCAATTTCTTTTCTTTTTTTTTCTATTAAAAAATATATTCAAATTTTTTTTTTTTAAATAACTCTTTATCTAAAAAAAAAACGACAATATGAAAATTTTTCTAGGAGTTAATGGCAAAAAAACGGCCTCCCGATAGGGaa                                   <  1:814369/150‑1 (MQ=255)
                                                                                                                    aCGTACATTTTCATTACCTTTCTTTTTTTTTCTATTTAAAATTATAATTAATTTTTTTTTTTTTAAATATCTATTTACCGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAg                                  <  1:8369817/150‑1 (MQ=255)
                                                                                                                       tACTTTTTAATTCCCTGTGTTTTTTTTTGTATTTAAAAATTTATTTATTTTTTTTTTTTTAAATTATCTATATAACGATCAAAAAAACGACAATATTAAATTTATTCGAGTTGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCg                               <  1:962052/150‑1 (MQ=255)
                                                                                                                       tACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCg                               <  2:560080/150‑1 (MQ=255)
                                                                                                                       tACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCg                               <  2:825227/150‑1 (MQ=255)
                                                                                                                         ctttttCATATCCTGTGTTTTTTTTTCTATTTCAAAATATAATTAAATTTTTTTTTTTTAAATATCGATATACCGATCAAAAAAACGAAAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTa                             <  1:1277669/148‑1 (MQ=255)
                                                                                                                         ctttttAATATCCTTTCTTTTTTTTTCGATTAAAAAATATAATTAAATTATTTTTTTTTAAATATCGATATAACTATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTa                             <  1:10067231/148‑1 (MQ=255)
                                                                                                                         cATTTTCCTATCCTGTCGTTTGTTGACGATTTCAAAATTCAATGAACTTATTTGTTTTTAAATATCGAGATAACGATCACAAACACGACAATATGACAATTATTCGAGGAGGGAAAGGCAAAAAAACGGCTTCCCTCTCGGGAGGCCGtt                             <  2:8042037/150‑2 (MQ=255)
                                                                                                                         atttttCTTATCTTGTTGTTTGTTTTCTATTTAAAATTATAATTAATTTTTTTTTTTTTAAATATCTATATAACTAACACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTa                             <  1:2927689/148‑1 (MQ=255)
                                                                                                                            tttCATATCCTGTCGTTTGTTTTCGATTTAAAAATATAATGAAAATATTTGTTTTTAAATATCGAGATAACCATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAAAGGCCTCCCGATAGGGAAGCCGTAGCa                          >  2:5314026/1‑150 (MQ=255)
                                                                                                                                atatCCTTTCGTTTTTTTTCTATTTCAAAATATAATTAAATTATTTTTTTTTAAATATCGATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGt                      <  1:7817786/150‑1 (MQ=255)
                                                                                                                                atagCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAACCGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGt                      <  2:7900121/150‑1 (MQ=255)
                                                                                                                                      tGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCgt                >  2:2284902/1‑150 (MQ=255)
                                                                                                                                      tGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCgt                <  2:2559628/150‑1 (MQ=255)
                                                                                                                                          gtttgtttTCGATTTCAAAATATAATTAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGttt            <  1:7133376/150‑1 (MQ=255)
                                                                                                                                             ttttttCTATTTCAAAATATAATTAATTTATTTTTTTTTAAATATCGTTATACCGATAAAAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTa         <  1:1870919/148‑1 (MQ=255)
                                                                                                                                             ttttttCTATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTa         <  1:8668544/148‑1 (MQ=255)
                                                                                                                                               ttttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATGACAAAATCGACAATTTGTATATTATTGGTGGTGTGTATGGCTATTTATCGGCGTCGGGATAGGGTAGGCGTAGGTAAGTGGGGGTGTTTTTTTg       >  2:6701337/1‑150 (MQ=255)
                                                                                                                                               ttttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATg       >  1:1413811/1‑150 (MQ=255)
                                                                                                                                                tttCTATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGc      <  2:9321180/150‑1 (MQ=255)
                                                                                                                                                tttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAATGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGc      <  2:1147253/150‑1 (MQ=255)
                                                                                                                                                 ttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGcc     >  2:7601055/1‑150 (MQ=255)
                                                                                                                                                 ttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGcc     >  1:10254325/1‑150 (MQ=255)
                                                                                                                                                  tCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCg    <  1:1584961/150‑1 (MQ=255)
                                                                                                                                                   cGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCgg   <  1:9919424/150‑1 (MQ=255)
                                                                                                                                                    gATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGa  <  2:1249633/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                       
GCAGGTTGGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGA  >  NC_000913/2177081‑2177378

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: