Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 164,576 | A→C | 16.7% | intergenic (+42/‑154) | hrpB → / → mrcB | putative ATP‑dependent helicase/fused glycosyl transferase and transpeptidase |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 164,576 | 0 | A | C | 16.7% | 535.8 / 21.3 | 272 | intergenic (+42/‑154) | hrpB/mrcB | putative ATP‑dependent helicase/fused glycosyl transferase and transpeptidase |
Reads supporting (aligned to +/- strand): ref base A (105/89); new base C (3/45); total (109/163) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 2.60e-10 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
Only 100 of 275 total aligned reads displayed.
GCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGA > NC_000913/164427‑164721 | cGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAg < 2:4449235/150‑1 (MQ=255) gTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAgg > 1:10040554/1‑150 (MQ=255) gTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAgg < 2:3233338/150‑1 (MQ=255) gTACCGTGAGGTGAAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAgg < 2:5837104/150‑1 (MQ=255) tACCGTGCGGTGCAAATCGAGATGAACGGGCGTTATCCCAACCAGGTCTGGCCGGACGCCCCGGCAAATACTGCACCGACGCGCCGGACGAAAACGTTTTCGTACGGTTGGTTTTCGCCCGCTCCCGGTGGGAGAGGGCCGGGGTGAggg < 2:966417/150‑1 (MQ=255) cggTGAGGTGCAAAAAGAGATGAACGGGCGTTATCCCAAACCTGTCTGGCCGGACGACCCGGAAAATACTGCACCGACGCGACGGACGAAAAAGTTTTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCa < 2:51897/148‑1 (MQ=255) ccGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGACAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCa > 1:5463539/1‑150 (MQ=255) ccGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCa < 2:51806/150‑1 (MQ=255) ccGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCa > 2:2391074/1‑150 (MQ=255) cGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCag > 1:5489914/1‑149 (MQ=255) cGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTAGTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGcct > 1:1451571/1‑148 (MQ=255) gTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATc > 1:6543634/1‑150 (MQ=255) tGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCa > 2:3112173/1‑150 (MQ=255) tGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCa > 2:5772187/1‑150 (MQ=255) ggTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAgcg > 1:1018042/1‑150 (MQ=255) gTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAgcgc > 1:7764284/1‑150 (MQ=255) gTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAgcgc > 2:7252915/1‑150 (MQ=255) tGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCcgcgcg > 2:6151943/1‑150 (MQ=255) aaGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGGGAGGGCCGGGGTGAGGGCATCAGCGCGCACGtg > 2:743108/1‑149 (MQ=255) agagATGAACGGGCTTTATCCCAAACTTGTCTGGCGGGACGACCCGGCAAATACTGCACCGACGCGAGGGACTAAAATGTATTCGTATGTTTGGTTTTCGCCCTCGCCCTGTGGGAGTGGGCCGGGGTGCGGGCATCAGCGCGCACGTtc < 2:8910086/150‑1 (MQ=255) tgttGAATGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGAAAATACTGCACCGACGCGACGGACGAAAATGTATTCGTACGGTTGGTTTTCTCCCCCTCCCTGTGGGAGTGGGCCGGGGTGCGGGCATCAGCGCGCACGTtcac < 2:6712214/147‑1 (MQ=255) agaTTAAAGGGGGTTACCCCAAACTGGTGGGGCCGGACGACCCGCCAAATACTCCCCGGACGCGACGGACTAAAATGTATTCGTATGGTTGTTTTTCTCCCTCTCCCTGTGGGTGTGGGCCGGGGTGCGGGCATCAGCGCGCACGTtcac < 2:476279/150‑1 (MQ=255) ttttattgggTGTTATCCAAAACTTGTGGGGCGGGCCGACCGGGAAAACACGGCACCGCCGGGAGGGACTAAAATGTTTGGGTCGGGTTGTTTTCCCCCCCCGCCGTGGGGGTGGGGGGGGGGGTGGGGGCATCAGCGCGCACGTtcacc < 2:3000888/143‑1 (MQ=255) tGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTggaccca > 1:9601694/1‑149 (MQ=255) gAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTtcaccctc > 2:7958573/1‑150 (MQ=255) gAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTgcacccgc > 1:5284801/1‑148 (MQ=255) gAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGGgcacccgc > 2:6443313/1‑148 (MQ=255) ggCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCAGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTgggcccccacccc > 1:3150405/1‑149 (MQ=38) ttATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCAGCAGCGCGCACGTGGACCCACACCCCAAcc > 1:6369974/1‑150 (MQ=38) taaccccAAACAGGTGGGGCCGGCCGACCCGGCAAATACTGCACCGCGGCGACGGACTAAAAAGTTTTGGTATGGTTGGTTTTCGCCCTCCCCGTGGGGGAGTGGGCGGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAAcc < 2:5616464/146‑1 (MQ=255) atattcaggcgcaaaagcccgattttctgttcttctgttacaagacagaagaagatatctctcaaaatttgtgctcgatcggtcccctctcccttgaGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAAcc > 2:6887016‑M1/98‑150 (MQ=255) ttccggcgcaaaagccctattttctgttcttctgttacaagacagaagaagatatctctcaaaatttgtgctcgatcggtcccctctccctggaGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCctc > 1:5770286‑M1/95‑150 (MQ=255) ccAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGCCCCCCCCCCCCAAccccccc > 1:2089773/1‑146 (MQ=38) cAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGGTCACCCACCCCCAAAcccacaac > 1:6112602/1‑145 (MQ=38) aCATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGGAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGCCCCCGCACCCGAACCCACGcccgc > 1:9840789/1‑148 (MQ=37) cATGTCGGGCCGTCCGCCCCGCAAAAAACGCCCCCGCCGCGACGGACTAAAATTTATTCGTATGTTTGGTTTACCCCCACCCCCGGGGGTAGAGGGCGGGGGTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCa < 2:2053471/150‑1 (MQ=255) ctgcgtGGCCGGACGACCCGGCAAATACGGCCCCGACGCGACGGACTAAAAAGTTTTCGTATGGTTGTTTTTCTCCCTCTCCCTGTGGGAGTGGGCCGGGGTGGGGCCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCaa < 2:5029656/145‑1 (MQ=37) ggtggggCGGGACGACCCGGAAAATACTGCCCCGACGCTCCGGACTAAAATTTTTTGTTATGGTTGTTTTTCTCCCTCTCCGTGGGGGTGTGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAg < 2:9255213/145‑1 (MQ=255) gTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAAACCTCTCCCTCAAgg > 2:1744447/1‑150 (MQ=255) tGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCGCAACCAAAACCAAACCCGCAAGGgag > 2:906256/1‑150 (MQ=37) gggccggccGCCCCGGCAAATACGGCCCCGCCGCGCCGGCCGAACAAGTATTCGTACGGTGGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGCGGGCCTCCGCGCGCCCGTTCACCCTCACCCTAACCCTCTCCCTTGAGGgag < 2:10037604/149‑1 (MQ=25) ccGGACGACCCTGCAAATCCTCCCCCGCCGCGCGGGCTGAAAATTTTTTCTTCTGTTTGGTTTCCGCCCTCGCCGTGGGGGTGCGGGCCGGGGTGTGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTTGAGGGAGAgg < 1:10424821/150‑1 (MQ=255) gacgacCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGCACCCGCACCCAAACCCACAACCACAAGGGAggggggg > 1:1841835/1‑149 (MQ=37) acgacCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCAGCAGCGCGCACGTGGGCCCGCACCCCAACCCACACCCACAAGGGGGAgggggg > 1:10818156/1‑148 (MQ=37) ccgtaaaaTCCTGCACCGCCGCGCGGGACCAAAACTTTTTGGTAGGGTTGGTTTGCCCCCCCCCCGTGGGGGTGCGGGCGGGGGGGCGGCCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATc < 2:3860258/145‑1 (MQ=255) cGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCACACCCGAAACCACACCCACAAGGGAGTGGGGACCCAtag > 2:2796032/1‑148 (MQ=37) gCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCCGCGCGCACGGGGGCCCGCCCCCAAAACCACACCCCCCAGCGAGCGGGGCCCGAACGAg > 1:683384/1‑150 (MQ=21) gCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCACACCCGCACCCACGCCCGCCCGGGAGAGGGGGCCGATCGAg > 2:9814813/1‑150 (MQ=37) cAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCAGCAGCGCGCACGTGCACCCACACCCCAAACCACGCCCACAACGGCGTGGGGGCCCCTCGAGc > 2:5982734/1‑150 (MQ=25) aaaTACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCGCACCCAAACCCACACCCACCAGGGCGAGGGGGCCGATCGAGcc > 1:4639582/1‑149 (MQ=37) cTGCCCCGCGGCGACGGACTAAAATGTAGTCGTATGGTTGGTTTTCTCCCTCCCCGGGGGGGCGTGGGCGGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAAt < 1:702286/150‑1 (MQ=255) cacccccgcgacgGACGAAAATTTATCCTCATGGTTTTTTAACCCCCTAGCCCTGTGTTGGAGGGCCGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAAtttt < 1:4045932/144‑1 (MQ=255) cACCGACGCGAGGGACGAAAAAGTTTTCGTATGTTGGGTTTTCTCCCTCCCCCTGTGGGAGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAAtttt < 2:7875394/150‑1 (MQ=38) aagacagaagaagatatctctcaaaatttgtgctcgatcggtcccctctcccttgaGGGAGAGGGTTAGGGTGAGGGCATCAGCGCGCACGTtcacc < 2:10262255‑M1/41‑1 (MQ=255) ccgccgCTAGGGACTAAAATGTATTCGTATTGTTGGTTTCCTCCCTCTCCGTGGGGGTGTGGGCGGGGGGGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTga < 1:8513688/150‑1 (MQ=255) ccgccgCGACGGACTAAAATGTTTTCGTATGTTTGTTTTTCTCCCTCTCCCTGTGGGAGTGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTga < 2:8476966/150‑1 (MQ=38) ccGACGCGACGGACTAAAAATTATTCGTATGTTTGTTTTTCTCCCTCTCCGTGGGGTCGCGGGCGGGGGTGTGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTga < 1:6056096/150‑1 (MQ=255) gacgcgacgcACTAAAATTTTTTCGTATGGTTGGTTGTCGCCCTCTCCCTGTGTGAGTGGGCGGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTgaga < 1:6852931/150‑1 (MQ=255) agaagaagatatctctcaaaatttgtgctcgatcggtcccctctcccttgaGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTa > 2:1535925‑M1/52‑101 (MQ=255) ggacgAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCCCCCACACCCCAACCCACAACCACAAGGGACAGGGGCCCGAACGACCCCAAAATTTTGGAGATAtctt > 2:10836548/1‑150 (MQ=21) gactAAAATGTTTTCGTATGGTTGTTTTTCTCCCTCTCCCTGTGGGTGTGGGCCGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtcttc < 2:800747/150‑1 (MQ=38) acgAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGCCCCCCACCCAAAAACACACCCACAAGGGGGCGGGGCCCGATCGAGCCCAAATTTTTAGATATAtcatat > 1:9941741/1‑146 (MQ=21) aaaaaGTATTCGTACGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtcttcttct < 2:10046251/150‑1 (MQ=255) aaaaaGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtcttcttct < 1:2686761/150‑1 (MQ=255) aaGTATTCGTATGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTc < 1:7958573/150‑1 (MQ=255) gTATTCGTAAGGTTGTTTTTCTCCCACTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCtt < 2:3618661/150‑1 (MQ=255) aTTCGTATGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGt < 1:7361234/150‑1 (MQ=255) tCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGGTCACCCTCAACCGAACCCACTCCCACAAGGGAGAGGGGACCGATCGAGCACAAAATTTGAGTGATATCTTATTCTGTGTTGTaa > 1:1001509/1‑150 (MQ=37) gTATGGTTGGTTTTCGCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACa < 1:10740866/150‑1 (MQ=255) tggttggttTTCTCCATCCCCGTGGGGGTGTGGGCGGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACagaa < 1:921273/149‑1 (MQ=255) cggttggttTTCCCCCTCTCCCTGTTGGAGCGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACagaa < 1:2910042/149‑1 (MQ=255) aGGTTGTTTTTCGCCCTCTCCCTGTGGGTTTGGGCGGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACagaa < 2:1281411/150‑1 (MQ=255) aGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACagaa < 2:2698439/150‑1 (MQ=255) gtttggttTTCGCCCGCTCCGTGGGGGTGTGGGCGGGGGTGAGGCCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACagaag < 2:187998/148‑1 (MQ=255) gttgtttTTCCCCCGCGCCCGGTGGGCGAGGGCCGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACagaaga < 2:5155676/150‑1 (MQ=255) gttggttTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGCACCCTCACCCGAACCCACCCCCTCAAGGGAGAGGGGACCGATCGCGCCCAAAGTTTGTGAGATATCTTCTTCTGTCTTGTGACagaaga > 1:8167551/1‑150 (MQ=37) ggttTACGCCCTCCCCGGGTGGGTGCGGGCCGGGGTGCGGCCCACAGCGCGCACGGTCACCCTCCCCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAACTTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACa < 1:9463696/150‑1 (MQ=255) ttgtcgccctctccctGTGGGAGTGGGCGGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGa < 1:1213530/144‑1 (MQ=255) ttCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCACACCCAAAACCACTACCGCAACGGGGAGGGGCCCCAACGAGCCCAAAATTTGTGAGATATATTATTATGTGTTGTAAAAGAAGAACAGaaa > 2:6396835/1‑150 (MQ=21) ctccctctccctGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCACACCCGAACCCACACCCGCAAGGGGGAGGGGCCCCCTAGCGCACAAATTTTGGGAGATATAATATTCAGTCTTGGTACAGAAGAACAGtaaaa > 2:4560660/1‑149 (MQ=19) tcccgctccgtGTGGGTGGGGGCGGGGGGGTGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATc < 1:4893053/150‑1 (MQ=255) ccccccccctGGGGGAGAGGGCCGGGGTGAGGGCATCAGCGAGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCg < 1:1031213/144‑1 (MQ=255) ctcgccgtggGGGAGTGGGGGGGGGTGCGGCCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCggg > 1:4144460‑M1/11‑150 (MQ=255) ctccccgtGTGGGATTGGGCGGGGGTGTGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCggg < 2:261030/150‑1 (MQ=255) gggGGGAGCGGGCCGGGGTGTGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTgc > 1:1947221‑M1/4‑150 (MQ=255) ggtcgcGGGCGGGGGTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGcc > 2:9552340‑M1/7‑150 (MQ=255) GGAGAGGGCCGGGGTGAGGCCATCAGCGCGCACGGTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCt > 1:2447466‑M1/1‑150 (MQ=255) gagaGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCACAACCAAAACCACACCCACAACGGGGAGGGGGCCGCAAGAGCACAAAATTTGAGAGATTTATTAGTATGTCTTGTAACAAAAGAACACAAAAAAGGGCTTTTTCGccag > 2:7087920/1‑148 (MQ=255) ggCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCACACCCAAACCCACTACCACCAGGGAGAGGGGACCGATCGAGCACAAATTTTTAGAGTTATAATATTAAGTAATTTAACAAAAGACACCAAAAACGGGCTTTTGAGCCGGAatat > 2:8221209/1‑150 (MQ=255) GCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCtctc < 2:2441762‑M1/50‑1 (MQ=255) cggggtTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTg < 2:240639/148‑1 (MQ=255) gggggTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGc < 1:2324145/149‑1 (MQ=255) ggggTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCg < 2:3382118/150‑1 (MQ=255) ggggTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCg < 2:8591153/150‑1 (MQ=255) ggggTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCg < 2:3534802/150‑1 (MQ=255) ggggTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCg < 2:8667172/150‑1 (MQ=255) ggggTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCg < 1:10570686/150‑1 (MQ=255) ggggTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCg < 1:10554851/150‑1 (MQ=255) ggTGTGGCCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGga < 2:1070668/150‑1 (MQ=255) ggTGAGGCCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGga < 1:355232/150‑1 (MQ=255) | GCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGA > NC_000913/164427‑164721 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |