Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 164,576 A→C 16.7% intergenic (+42/‑154) hrpB → / → mrcB putative ATP‑dependent helicase/fused glycosyl transferase and transpeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913164,5760AC16.7% 535.8 / 21.3 272intergenic (+42/‑154)hrpB/mrcBputative ATP‑dependent helicase/fused glycosyl transferase and transpeptidase
Reads supporting (aligned to +/- strand):  ref base A (105/89);  new base C (3/45);  total (109/163)
Fisher's exact test for biased strand distribution p-value = 2.60e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 275 total aligned reads displayed.

GCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGA  >  NC_000913/164427‑164721
                                                                                                                                                     |                                                                                                                                                 
 cGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAg                                                                                                                                                  <  2:4449235/150‑1 (MQ=255)
  gTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAgg                                                                                                                                                 >  1:10040554/1‑150 (MQ=255)
  gTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAgg                                                                                                                                                 <  2:3233338/150‑1 (MQ=255)
  gTACCGTGAGGTGAAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAgg                                                                                                                                                 <  2:5837104/150‑1 (MQ=255)
   tACCGTGCGGTGCAAATCGAGATGAACGGGCGTTATCCCAACCAGGTCTGGCCGGACGCCCCGGCAAATACTGCACCGACGCGCCGGACGAAAACGTTTTCGTACGGTTGGTTTTCGCCCGCTCCCGGTGGGAGAGGGCCGGGGTGAggg                                                                                                                                                <  2:966417/150‑1 (MQ=255)
     cggTGAGGTGCAAAAAGAGATGAACGGGCGTTATCCCAAACCTGTCTGGCCGGACGACCCGGAAAATACTGCACCGACGCGACGGACGAAAAAGTTTTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCa                                                                                                                                              <  2:51897/148‑1 (MQ=255)
     ccGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGACAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCa                                                                                                                                              >  1:5463539/1‑150 (MQ=255)
     ccGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCa                                                                                                                                              <  2:51806/150‑1 (MQ=255)
     ccGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCa                                                                                                                                              >  2:2391074/1‑150 (MQ=255)
      cGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCag                                                                                                                                             >  1:5489914/1‑149 (MQ=255)
      cGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTAGTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGcct                                                                                                                                             >  1:1451571/1‑148 (MQ=255)
       gTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATc                                                                                                                                            >  1:6543634/1‑150 (MQ=255)
        tGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCa                                                                                                                                           >  2:3112173/1‑150 (MQ=255)
        tGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCa                                                                                                                                           >  2:5772187/1‑150 (MQ=255)
           ggTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAgcg                                                                                                                                        >  1:1018042/1‑150 (MQ=255)
            gTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAgcgc                                                                                                                                       >  1:7764284/1‑150 (MQ=255)
            gTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAgcgc                                                                                                                                       >  2:7252915/1‑150 (MQ=255)
             tGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCcgcgcg                                                                                                                                      >  2:6151943/1‑150 (MQ=255)
                   aaGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGGGAGGGCCGGGGTGAGGGCATCAGCGCGCACGtg                                                                                                                                >  2:743108/1‑149 (MQ=255)
                    agagATGAACGGGCTTTATCCCAAACTTGTCTGGCGGGACGACCCGGCAAATACTGCACCGACGCGAGGGACTAAAATGTATTCGTATGTTTGGTTTTCGCCCTCGCCCTGTGGGAGTGGGCCGGGGTGCGGGCATCAGCGCGCACGTtc                                                                                                                               <  2:8910086/150‑1 (MQ=255)
                      tgttGAATGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGAAAATACTGCACCGACGCGACGGACGAAAATGTATTCGTACGGTTGGTTTTCTCCCCCTCCCTGTGGGAGTGGGCCGGGGTGCGGGCATCAGCGCGCACGTtcac                                                                                                                             <  2:6712214/147‑1 (MQ=255)
                      agaTTAAAGGGGGTTACCCCAAACTGGTGGGGCCGGACGACCCGCCAAATACTCCCCGGACGCGACGGACTAAAATGTATTCGTATGGTTGTTTTTCTCCCTCTCCCTGTGGGTGTGGGCCGGGGTGCGGGCATCAGCGCGCACGTtcac                                                                                                                             <  2:476279/150‑1 (MQ=255)
                       ttttattgggTGTTATCCAAAACTTGTGGGGCGGGCCGACCGGGAAAACACGGCACCGCCGGGAGGGACTAAAATGTTTGGGTCGGGTTGTTTTCCCCCCCCGCCGTGGGGGTGGGGGGGGGGGTGGGGGCATCAGCGCGCACGTtcacc                                                                                                                            <  2:3000888/143‑1 (MQ=255)
                         tGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTggaccca                                                                                                                          >  1:9601694/1‑149 (MQ=255)
                          gAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTtcaccctc                                                                                                                         >  2:7958573/1‑150 (MQ=255)
                          gAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTgcacccgc                                                                                                                         >  1:5284801/1‑148 (MQ=255)
                          gAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGGgcacccgc                                                                                                                         >  2:6443313/1‑148 (MQ=255)
                               ggCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCAGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTgggcccccacccc                                                                                                                    >  1:3150405/1‑149 (MQ=38)
                                   ttATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCAGCAGCGCGCACGTGGACCCACACCCCAAcc                                                                                                                >  1:6369974/1‑150 (MQ=38)
                                   taaccccAAACAGGTGGGGCCGGCCGACCCGGCAAATACTGCACCGCGGCGACGGACTAAAAAGTTTTGGTATGGTTGGTTTTCGCCCTCCCCGTGGGGGAGTGGGCGGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAAcc                                                                                                                <  2:5616464/146‑1 (MQ=255)
                                   atattcaggcgcaaaagcccgattttctgttcttctgttacaagacagaagaagatatctctcaaaatttgtgctcgatcggtcccctctcccttgaGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAAcc                                                                                                                >  2:6887016‑M1/98‑150 (MQ=255)
                                      ttccggcgcaaaagccctattttctgttcttctgttacaagacagaagaagatatctctcaaaatttgtgctcgatcggtcccctctccctggaGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCctc                                                                                                             >  1:5770286‑M1/95‑150 (MQ=255)
                                        ccAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGCCCCCCCCCCCCAAccccccc                                                                                                           >  1:2089773/1‑146 (MQ=38)
                                         cAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGGTCACCCACCCCCAAAcccacaac                                                                                                          >  1:6112602/1‑145 (MQ=38)
                                            aCATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGGAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGCCCCCGCACCCGAACCCACGcccgc                                                                                                       >  1:9840789/1‑148 (MQ=37)
                                             cATGTCGGGCCGTCCGCCCCGCAAAAAACGCCCCCGCCGCGACGGACTAAAATTTATTCGTATGTTTGGTTTACCCCCACCCCCGGGGGTAGAGGGCGGGGGTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCa                                                                                                      <  2:2053471/150‑1 (MQ=255)
                                              ctgcgtGGCCGGACGACCCGGCAAATACGGCCCCGACGCGACGGACTAAAAAGTTTTCGTATGGTTGTTTTTCTCCCTCTCCCTGTGGGAGTGGGCCGGGGTGGGGCCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCaa                                                                                                     <  2:5029656/145‑1 (MQ=37)
                                               ggtggggCGGGACGACCCGGAAAATACTGCCCCGACGCTCCGGACTAAAATTTTTTGTTATGGTTGTTTTTCTCCCTCTCCGTGGGGGTGTGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAg                                                                                                    <  2:9255213/145‑1 (MQ=255)
                                                gTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAAACCTCTCCCTCAAgg                                                                                                   >  2:1744447/1‑150 (MQ=255)
                                                   tGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCGCAACCAAAACCAAACCCGCAAGGgag                                                                                                >  2:906256/1‑150 (MQ=37)
                                                   gggccggccGCCCCGGCAAATACGGCCCCGCCGCGCCGGCCGAACAAGTATTCGTACGGTGGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGCGGGCCTCCGCGCGCCCGTTCACCCTCACCCTAACCCTCTCCCTTGAGGgag                                                                                                <  2:10037604/149‑1 (MQ=25)
                                                      ccGGACGACCCTGCAAATCCTCCCCCGCCGCGCGGGCTGAAAATTTTTTCTTCTGTTTGGTTTCCGCCCTCGCCGTGGGGGTGCGGGCCGGGGTGTGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTTGAGGGAGAgg                                                                                             <  1:10424821/150‑1 (MQ=255)
                                                         gacgacCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGCACCCGCACCCAAACCCACAACCACAAGGGAggggggg                                                                                          >  1:1841835/1‑149 (MQ=37)
                                                          acgacCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCAGCAGCGCGCACGTGGGCCCGCACCCCAACCCACACCCACAAGGGGGAgggggg                                                                                         >  1:10818156/1‑148 (MQ=37)
                                                               ccgtaaaaTCCTGCACCGCCGCGCGGGACCAAAACTTTTTGGTAGGGTTGGTTTGCCCCCCCCCCGTGGGGGTGCGGGCGGGGGGGCGGCCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATc                                                                                    <  2:3860258/145‑1 (MQ=255)
                                                                cGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCACACCCGAAACCACACCCACAAGGGAGTGGGGACCCAtag                                                                                   >  2:2796032/1‑148 (MQ=37)
                                                                  gCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCCGCGCGCACGGGGGCCCGCCCCCAAAACCACACCCCCCAGCGAGCGGGGCCCGAACGAg                                                                                 >  1:683384/1‑150 (MQ=21)
                                                                  gCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCACACCCGCACCCACGCCCGCCCGGGAGAGGGGGCCGATCGAg                                                                                 >  2:9814813/1‑150 (MQ=37)
                                                                   cAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCAGCAGCGCGCACGTGCACCCACACCCCAAACCACGCCCACAACGGCGTGGGGGCCCCTCGAGc                                                                                >  2:5982734/1‑150 (MQ=25)
                                                                    aaaTACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCGCACCCAAACCCACACCCACCAGGGCGAGGGGGCCGATCGAGcc                                                                               >  1:4639582/1‑149 (MQ=37)
                                                                         cTGCCCCGCGGCGACGGACTAAAATGTAGTCGTATGGTTGGTTTTCTCCCTCCCCGGGGGGGCGTGGGCGGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAAt                                                                          <  1:702286/150‑1 (MQ=255)
                                                                            cacccccgcgacgGACGAAAATTTATCCTCATGGTTTTTTAACCCCCTAGCCCTGTGTTGGAGGGCCGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAAtttt                                                                       <  1:4045932/144‑1 (MQ=255)
                                                                            cACCGACGCGAGGGACGAAAAAGTTTTCGTATGTTGGGTTTTCTCCCTCCCCCTGTGGGAGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAAtttt                                                                       <  2:7875394/150‑1 (MQ=38)
                                                                            aagacagaagaagatatctctcaaaatttgtgctcgatcggtcccctctcccttgaGGGAGAGGGTTAGGGTGAGGGCATCAGCGCGCACGTtcacc                                                                                                                            <  2:10262255‑M1/41‑1 (MQ=255)
                                                                              ccgccgCTAGGGACTAAAATGTATTCGTATTGTTGGTTTCCTCCCTCTCCGTGGGGGTGTGGGCGGGGGGGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTga                                                                     <  1:8513688/150‑1 (MQ=255)
                                                                              ccgccgCGACGGACTAAAATGTTTTCGTATGTTTGTTTTTCTCCCTCTCCCTGTGGGAGTGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTga                                                                     <  2:8476966/150‑1 (MQ=38)
                                                                              ccGACGCGACGGACTAAAAATTATTCGTATGTTTGTTTTTCTCCCTCTCCGTGGGGTCGCGGGCGGGGGTGTGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTga                                                                     <  1:6056096/150‑1 (MQ=255)
                                                                                gacgcgacgcACTAAAATTTTTTCGTATGGTTGGTTGTCGCCCTCTCCCTGTGTGAGTGGGCGGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTgaga                                                                   <  1:6852931/150‑1 (MQ=255)
                                                                                 agaagaagatatctctcaaaatttgtgctcgatcggtcccctctcccttgaGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTa                                                                                                                   >  2:1535925‑M1/52‑101 (MQ=255)
                                                                                        ggacgAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCCCCCACACCCCAACCCACAACCACAAGGGACAGGGGCCCGAACGACCCCAAAATTTTGGAGATAtctt                                                           >  2:10836548/1‑150 (MQ=21)
                                                                                         gactAAAATGTTTTCGTATGGTTGTTTTTCTCCCTCTCCCTGTGGGTGTGGGCCGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtcttc                                                          <  2:800747/150‑1 (MQ=38)
                                                                                          acgAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGCCCCCCACCCAAAAACACACCCACAAGGGGGCGGGGCCCGATCGAGCCCAAATTTTTAGATATAtcatat                                                         >  1:9941741/1‑146 (MQ=21)
                                                                                             aaaaaGTATTCGTACGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtcttcttct                                                      <  2:10046251/150‑1 (MQ=255)
                                                                                             aaaaaGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtcttcttct                                                      <  1:2686761/150‑1 (MQ=255)
                                                                                                aaGTATTCGTATGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTc                                                   <  1:7958573/150‑1 (MQ=255)
                                                                                                  gTATTCGTAAGGTTGTTTTTCTCCCACTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCtt                                                 <  2:3618661/150‑1 (MQ=255)
                                                                                                    aTTCGTATGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGt                                               <  1:7361234/150‑1 (MQ=255)
                                                                                                      tCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGGTCACCCTCAACCGAACCCACTCCCACAAGGGAGAGGGGACCGATCGAGCACAAAATTTGAGTGATATCTTATTCTGTGTTGTaa                                             >  1:1001509/1‑150 (MQ=37)
                                                                                                        gTATGGTTGGTTTTCGCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACa                                           <  1:10740866/150‑1 (MQ=255)
                                                                                                           tggttggttTTCTCCATCCCCGTGGGGGTGTGGGCGGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACagaa                                        <  1:921273/149‑1 (MQ=255)
                                                                                                           cggttggttTTCCCCCTCTCCCTGTTGGAGCGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACagaa                                        <  1:2910042/149‑1 (MQ=255)
                                                                                                           aGGTTGTTTTTCGCCCTCTCCCTGTGGGTTTGGGCGGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACagaa                                        <  2:1281411/150‑1 (MQ=255)
                                                                                                           aGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACagaa                                        <  2:2698439/150‑1 (MQ=255)
                                                                                                            gtttggttTTCGCCCGCTCCGTGGGGGTGTGGGCGGGGGTGAGGCCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACagaag                                       <  2:187998/148‑1 (MQ=255)
                                                                                                             gttgtttTTCCCCCGCGCCCGGTGGGCGAGGGCCGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACagaaga                                      <  2:5155676/150‑1 (MQ=255)
                                                                                                             gttggttTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGCACCCTCACCCGAACCCACCCCCTCAAGGGAGAGGGGACCGATCGCGCCCAAAGTTTGTGAGATATCTTCTTCTGTCTTGTGACagaaga                                      >  1:8167551/1‑150 (MQ=37)
                                                                                                                ggttTACGCCCTCCCCGGGTGGGTGCGGGCCGGGGTGCGGCCCACAGCGCGCACGGTCACCCTCCCCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAACTTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACa                                   <  1:9463696/150‑1 (MQ=255)
                                                                                                                  ttgtcgccctctccctGTGGGAGTGGGCGGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGa                                 <  1:1213530/144‑1 (MQ=255)
                                                                                                                    ttCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCACACCCAAAACCACTACCGCAACGGGGAGGGGCCCCAACGAGCCCAAAATTTGTGAGATATATTATTATGTGTTGTAAAAGAAGAACAGaaa                               >  2:6396835/1‑150 (MQ=21)
                                                                                                                      ctccctctccctGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCACACCCGAACCCACACCCGCAAGGGGGAGGGGCCCCCTAGCGCACAAATTTTGGGAGATATAATATTCAGTCTTGGTACAGAAGAACAGtaaaa                             >  2:4560660/1‑149 (MQ=19)
                                                                                                                       tcccgctccgtGTGGGTGGGGGCGGGGGGGTGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATc                            <  1:4893053/150‑1 (MQ=255)
                                                                                                                        ccccccccctGGGGGAGAGGGCCGGGGTGAGGGCATCAGCGAGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCg                           <  1:1031213/144‑1 (MQ=255)
                                                                                                                          ctcgccgtggGGGAGTGGGGGGGGGTGCGGCCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCggg                         >  1:4144460‑M1/11‑150 (MQ=255)
                                                                                                                          ctccccgtGTGGGATTGGGCGGGGGTGTGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCggg                         <  2:261030/150‑1 (MQ=255)
                                                                                                                                 gggGGGAGCGGGCCGGGGTGTGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTgc                  >  1:1947221‑M1/4‑150 (MQ=255)
                                                                                                                                    ggtcgcGGGCGGGGGTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGcc               >  2:9552340‑M1/7‑150 (MQ=255)
                                                                                                                                     GGAGAGGGCCGGGGTGAGGCCATCAGCGCGCACGGTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCt              >  1:2447466‑M1/1‑150 (MQ=255)
                                                                                                                                      gagaGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCACAACCAAAACCACACCCACAACGGGGAGGGGGCCGCAAGAGCACAAAATTTGAGAGATTTATTAGTATGTCTTGTAACAAAAGAACACAAAAAAGGGCTTTTTCGccag             >  2:7087920/1‑148 (MQ=255)
                                                                                                                                           ggCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCACACCCAAACCCACTACCACCAGGGAGAGGGGACCGATCGAGCACAAATTTTTAGAGTTATAATATTAAGTAATTTAACAAAAGACACCAAAAACGGGCTTTTGAGCCGGAatat        >  2:8221209/1‑150 (MQ=255)
                                                                                                                                            GCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCtctc                                                                                                           <  2:2441762‑M1/50‑1 (MQ=255)
                                                                                                                                             cggggtTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTg      <  2:240639/148‑1 (MQ=255)
                                                                                                                                              gggggTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGc     <  1:2324145/149‑1 (MQ=255)
                                                                                                                                               ggggTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCg    <  2:3382118/150‑1 (MQ=255)
                                                                                                                                               ggggTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCg    <  2:8591153/150‑1 (MQ=255)
                                                                                                                                               ggggTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCg    <  2:3534802/150‑1 (MQ=255)
                                                                                                                                               ggggTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCg    <  2:8667172/150‑1 (MQ=255)
                                                                                                                                               ggggTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCg    <  1:10570686/150‑1 (MQ=255)
                                                                                                                                               ggggTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCg    <  1:10554851/150‑1 (MQ=255)
                                                                                                                                                 ggTGTGGCCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGga  <  2:1070668/150‑1 (MQ=255)
                                                                                                                                                 ggTGAGGCCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGga  <  1:355232/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                 
GCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGA  >  NC_000913/164427‑164721

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: