Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 164,620 | C→T | 5.2% | intergenic (+86/‑110) | hrpB → / → mrcB | putative ATP‑dependent helicase/fused glycosyl transferase and transpeptidase |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 164,620 | 0 | C | T | 5.2% | 958.7 / 22.3 | 287 | intergenic (+86/‑110) | hrpB/mrcB | putative ATP‑dependent helicase/fused glycosyl transferase and transpeptidase |
Reads supporting (aligned to +/- strand): ref base C (107/165); new base T (7/8); total (114/173) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 5.96e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
Only 100 of 290 total aligned reads displayed.
CATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGC‑ACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGGG > NC_000913/164472‑164768 | cATGTCGGGCCGTCCGCCCCGCAAAAAACGCCCCCGCCGCGACGGACTAAAATTTATTCGTATGTTTGGTTTACCCCCACCCCCGGGGGTAGAGGGCGGGGGTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCa < 2:2053471/150‑1 (MQ=255) tGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGAAAGTTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAg < 2:9084564/150‑1 (MQ=255) gtggggCCGGACGCCCGGCCAAATAGGGCCCCGACGCGCGGTCCTAAAATTTTTTGGTAGGGTTGTTTTTCCCCGTCTCCCGGGGGGTGTGGGCGGGGGTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAgg < 2:3045612/146‑1 (MQ=255) gTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAAACCTCTCCCTCAAgg > 2:1744447/1‑150 (MQ=255) cTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGGGGGAGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGga < 2:2665951/150‑1 (MQ=255) tGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCACACCCGAACCCACACCCACAAGGgag > 1:9825867/1‑150 (MQ=38) ccGTACGACCCGTAAAAAACTGCACCGACGCGCCGGACTAAAATGTATTCGTATGGTTGTTTTACCCCCTCCCCGTGTGGGTGCGGGCGGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAgg < 2:5006582/150‑1 (MQ=255) ccGGACGACCCGGCAAATACTGCACCGACGCGACGGACTAAAAAGTATTCGTACGGTTGGTTTTCTCCCTCTCCCTGTGGGCGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTTGAGGGAGAgg < 1:9879184/150‑1 (MQ=38) gacgacCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCACACCCTAACCCACTCCCACAAGGGGGAggggg > 2:3662965/1‑149 (MQ=255) acgacCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCAGCAGCGCGCACGTGGGCCCGCACCCCAACCCACACCCACAAGGGGGAgggggg > 1:10818156/1‑148 (MQ=37) gacCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCCACCCCAAAACCACAACCACCAGGGGGAGGGGGCCg > 1:1865546/1‑150 (MQ=25) gCAAATCCGGCCCCGACGCGCCGGACGAAAAAGTATTCGTACGGTTGGTTTTCTCCCCCCCCCGGGGGGAGAGGGCCGGGGTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAg < 2:5811129/150‑1 (MQ=255) gCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCCGCGCGCACGGGGGCCCGCCCCCAAAACCACACCCCCCAGCGAGCGGGGCCCGAACGAg > 1:683384/1‑150 (MQ=21) cAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCAGCAGCGCGCACGTGCACCCACACCCCAAACCACGCCCACAACGGCGTGGGGGCCCCTCGAGc > 2:5982734/1‑150 (MQ=25) aaaTACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCGCACCCAAACCCACACCCACCAGGGCGAGGGGGCCGATCGAGcc > 1:4639582/1‑149 (MQ=37) cTGCCCCGCGGCGACGGACTAAAATGTAGTCGTATGGTTGGTTTTCTCCCTCCCCGGGGGGGCGTGGGCGGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGC‑ACAAAt < 1:702286/150‑1 (MQ=255) gCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGCGCCCACACCCACACCCGCTCCCGCCCGGGAGAGGGGGCCGAGCGCGC‑CCAAAatt > 2:10851448/1‑150 (MQ=25) cACCGCCGCGCCGGAAGAAAAAGTATTCTTATGGTTGGTTTTCCCCCCCTCCCTGTGGGAGCGGGCCGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGC‑ACAAAtttt < 1:221568/150‑1 (MQ=255) cACCGACGCGAGGGACGAAAAAGTTTTCGTATGTTGGGTTTTCTCCCTCCCCCTGTGGGAGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGC‑ACAAAtttt < 2:7875394/150‑1 (MQ=38) ccgccgCTAGGGACTAAAATGTATTCGTATTGTTGGTTTCCTCCCTCTCCGTGGGGGTGTGGGCGGGGGGGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGC‑ACAAATTTTga < 1:8513688/150‑1 (MQ=255) ccgccgCGACGGACTAAAATGTTTTCGTATGTTTGTTTTTCTCCCTCTCCCTGTGGGAGTGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGC‑ACAAATTTTga < 2:8476966/150‑1 (MQ=38) cccacgcgacgCACCAAAAAGTATTCGAAAGGTTGGTTTACACCCACCCCCTGTGGCAGAGGGCCGGGGTGAGGCAATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGC‑ACAAATTTTga < 2:8796251/147‑1 (MQ=255) ccGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGC‑ACAAATTTTga < 2:8214071/150‑1 (MQ=255) gacgcgacgcACTAAAATTTTTTCGTATGGTTGGTTGTCGCCCTCTCCCTGTGTGAGTGGGCGGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGC‑ACAAATTTTgaga < 1:6852931/150‑1 (MQ=255) acgcgacgGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCACACCCAAAACCACAACCACAAGGGAGAGTGGACCCAACGAGC‑ACAAAATTTtagag > 2:2789576/1‑150 (MQ=25) gactAAAATGTTTTCGTATGGTTGTTTTTCTCCCTCTCCCTGTGGGTGTGGGCCGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGC‑ACAAATTTTGAGAGATAtcttc < 2:800747/150‑1 (MQ=38) acgAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGCCCCCCACCCAAAAACACACCCACAAGGGGGCGGGGCCCGATCGAGC‑CCAAATTTTTAGATATAtcatat > 1:9941741/1‑146 (MQ=21) cgAAAAAGTATTCTTACGGTTGGTTTCCCCCCTCTCCCTGTGGCAGAGGCCGGGGGTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGC‑ACAAATTTTGAGAGATAtcttcta < 1:5734634/150‑2 (MQ=255) aaaacGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGC‑ACAAATTTTGAGAGATAtcttcttct < 2:10046109/150‑1 (MQ=255) aaaaGTAATCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCCACCACCCCCAAAACCACTCCCACAAGGGCGACGGGCCCCATCCAGCAACAAATTTT‑CGAGATAtcttcttcgg > 2:6043175/1‑148 (MQ=16) aTTCGTATGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGC‑ACAAATTTTGAGAGATATCTTCTTCTGTCTTGt < 1:7361234/150‑1 (MQ=255) ttCGTATGGTTGGTTTTCTCCCTCTCCCTGTGGTAGTGGGCGGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGC‑ACAAATTTTGAGAGATATCTTCTTCTGTCTTGTa < 1:5805344/150‑1 (MQ=255) tCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGGTCACCCTCAACCGAACCCACTCCCACAAGGGAGAGGGGACCGATCGAGC‑ACAAAATTTGAGTGATATCTTATTCTGTGTTGTaa > 1:1001509/1‑150 (MQ=37) gtttggttgtttTTCTCCCTCTCCCTGGGGGTGTGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGC‑ACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACa < 2:683384/146‑1 (MQ=39) gTACGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGC‑ACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACa < 1:10740861/150‑1 (MQ=255) tggttggttTTCTCCATCCCCGTGGGGGTGTGGGCGGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGC‑ACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACagaa < 1:921273/149‑1 (MQ=255) gtttggttTTCGCCCGCTCCGTGGGGGTGTGGGCGGGGGTGAGGCCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGC‑ACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACagaag < 2:187998/148‑1 (MQ=255) gttgtttTTCCCCCCCCCCCTGTGGGAGCGGGCGGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGC‑ACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACagaaga < 2:243285/150‑1 (MQ=255) gttggtcTTCCCCCCCCCCCTGGGGGTGGGGGCCGGGGGGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGC‑ACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACagaaga < 1:3201849/150‑1 (MQ=255) ttggttTTCTCCCTCGCCGGGGGGGTGTGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGC‑ACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAgaagaa < 1:9375051/150‑1 (MQ=255) ttgtcgccctctccctGTGGGAGTGGGCGGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGC‑ACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGa < 1:1213530/144‑1 (MQ=255) tcccgctccgtGTGGGTGGGGGCGGGGGGGTGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGC‑ACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATc < 1:4893053/150‑1 (MQ=255) ccctctccgtGTGGTTGTGGGGGGGGGTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGC‑ACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCg < 1:4118024/150‑1 (MQ=255) ccccccccctGGGGGAGAGGGCCGGGGTGAGGGCATCAGCGAGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGC‑ACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCg < 1:1031213/144‑1 (MQ=255) cctctccctGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGC‑ACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCgg < 1:3667211/150‑1 (MQ=255) cctGTGGGTGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGC‑ACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCtttt < 1:10034739/150‑1 (MQ=255) tgtgGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGGGGGGACCGATCGAGC‑ACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTgc > 2:7051134/1‑150 (MQ=255) ggggcgcgggCGGGGGTGCGGCCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGC‑ACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTgcgc < 1:4665968/143‑1 (MQ=255) GGAGAGGGCCGGGGTGAGGCCATCAGCGCGCACGGTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGC‑ACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCt > 1:2447466‑M1/1‑150 (MQ=255) GAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGC‑ACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTg > 2:3578751‑M1/1‑150 (MQ=255) tagggtgagggtgaacgtgcgcgctgatgccctcaccccggccctctcccTCAAGGGAGAGGGGACCGATCGAGC‑ACAAATTTTGAGAGATAtcttcttct < 1:1535925‑M2/51‑1 (MQ=255) cGGGGTGCGGGCATCAGCGCGCACGGTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGC‑ACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGc < 2:5182419/150‑1 (MQ=255) ggggTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGC‑ACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCg < 2:3382118/150‑1 (MQ=255) ggggTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGC‑ACAAATTTTGAGAGATACCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCg < 1:1308967/150‑1 (MQ=255) ggggTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGC‑ACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCg < 2:8591153/150‑1 (MQ=255) ggggTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGC‑ACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCg < 1:10570686/150‑1 (MQ=255) gggTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGC‑ACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCgg < 1:10426756/150‑1 (MQ=255) gggTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGC‑ACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCgg < 1:2796032/150‑1 (MQ=255) ggTGTGGCCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGC‑ACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGga < 2:1070668/150‑1 (MQ=255) ggCCTCAGCGCGCACGTTCACCCTCACCCGACCCCTCTCCCTCAAGGGAGAGGGGACCGAGCGAGC‑ACAAATTTTGAGAGATATCTTCTTCGGTCGTGTAACCGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGaaaaa < 2:10539784/150‑1 (MQ=255) ggCATCAGCGCGCACGTTCACTCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGC‑ACAAATTTTGAGATATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGaaaaa < 1:515053/150‑1 (MQ=255) cATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGC‑ACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGc < 2:5600345/150‑1 (MQ=255) tCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGC‑ACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCAt < 1:3017088/150‑1 (MQ=255) cAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGC‑ACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATg < 2:4019798/150‑1 (MQ=255) gcgcACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGC‑ACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCg < 2:9695559/150‑1 (MQ=255) cgcACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGC‑ACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCgg < 1:2032490/150‑1 (MQ=255) cgcACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGC‑ACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCgg < 1:775597/150‑1 (MQ=255) gcACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGC‑ACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTAAATATTGCGGAGAAAAAGCATGGCCggg < 2:6434818/150‑1 (MQ=255) aCGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGC‑ACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGaa < 2:898655/150‑1 (MQ=255) ttCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGC‑ACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGa < 2:10147577/150‑1 (MQ=255) tcaccctcacccAAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGC‑ACAAAATTTGAGAGATATCTTATTATGTCTTTTAAAAGAAGAACAGAAAAACGGGCTTTTTAGCCGGAAAATTTCGGAGAAAAATCAAGGGCGGGAATGAc > 2:7234719/1‑150 (MQ=255) ctcaccctAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGC‑ACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGa < 1:553819/150‑1 (MQ=255) ctcaccctAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGC‑ACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGa > 2:6343058/1‑150 (MQ=255) ctcaccctAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGC‑ACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGa < 1:805550/150‑1 (MQ=255) atcggtcccctctcccTTGAGGGAGAGGGGACCGATCGAGC‑ACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGcc > 2:5457252‑M2/17‑150 (MQ=255) accctAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGC‑ACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGcc > 2:505458/1‑150 (MQ=255) cctAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGC‑ACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCaa > 2:2834560/1‑150 (MQ=255) cctAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGC‑ACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCaa > 1:3096424/1‑150 (MQ=255) cctAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGC‑ACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCaa > 1:9064566/1‑150 (MQ=255) tAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGC‑ACAAATTTTGAGAGATATCTTCGTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAAtt < 1:4403460/150‑1 (MQ=255) aaCCCTCTCCCTCACGGGAGAGGGGACCGATCGAGC‑CCAAATTTTGAGAGATATCTTCTTCTGTCGTGGAACAGAAGAACAGAAAATCGGGCTGTTGCGCCTGAAGATTGCGGAGAAAAAGCATGGCCGGGACTGACCGCGAGccccttg > 2:982554/1‑145 (MQ=255) ccctctcccTCAAGGGAGAGGGGACCGATCGAGC‑ACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGa < 1:8221602‑M2/141‑1 (MQ=255) cctctcccTCAAGGGAGAGGGGACCGATCGAGC‑ACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGCCCGCGAGCCACTTGGAc > 1:4289189‑M2/9‑150 (MQ=255) ctctcccTCAAGGGAGAGGGGACCGATCGAGC‑ACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCgg < 2:8704012‑M2/119‑1 (MQ=255) ctctcccTCAAGGGAGAGGGGACCGATCGAGC‑ACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACg < 2:9297903‑M2/143‑1 (MQ=255) ctctcccTCAAGGGAGAGGGGACCGATCGAGC‑ACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACg > 1:6116233‑M2/8‑150 (MQ=255) tctcccTCAAGGGAGAGGGGACCGATCGAGC‑ACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGc > 1:6046642‑M2/7‑150 (MQ=255) tctcccTCAAGGGAGAGGGGACCGATCGAGC‑ACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGc > 1:6564157‑M2/7‑150 (MQ=255) tcccTCAAGGGAGAGGGGACCGATCGAGC‑ACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCaa < 1:5459299‑M2/146‑1 (MQ=255) ccTCAAGGGAGAGGGGACCGATCGAGC‑ACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGACTGACCGCGAGCCCATTGGACGCAAAg > 1:10270633‑M2/3‑150 (MQ=255) ccTCAAGGGAGAGGGGACCGATCGAGC‑ACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAg < 1:5655117‑M2/148‑1 (MQ=255) cTCAAGGGAGAGGGGACCGATCGAGC‑ACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAgg > 1:743777‑M2/2‑150 (MQ=255) cTCAAGGGAGAGGGGACCGATCGAGC‑ACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAgg < 2:1323307‑M2/149‑1 (MQ=255) cTCAAGGGAGAGGGGACCGATCGAGC‑ACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAgg > 1:6300181‑M2/2‑150 (MQ=255) tCAAGGGAGAGGGGACCGATCGAGC‑ACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGc > 2:4536757/1‑100 (MQ=255) tCAAGGGAGAGGGGACCGATCGAGC‑ACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAACAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAggg > 2:7682080/1‑150 (MQ=255) tCAAGGGAGAGGGGACCGATCGAGC‑ACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAggg < 2:1498320/150‑1 (MQ=255) tCAAGGGAGAGGGGACCGATCGAGC‑ACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAggg > 1:1752000/1‑150 (MQ=255) tCAAGGGAGAGGGGACCGATCGAGC‑ACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAggg > 1:887688/1‑150 (MQ=255) cAAGGGAGAGGGGACCGATCGAGC‑ACAAATTTTGAGAGATATCTTCTTCTGTCt > 2:4651664/1‑54 (MQ=255) | CATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGC‑ACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGGG > NC_000913/164472‑164768 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |