Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,270,101 C→A 5.9% intergenic (+499/+115) yhaC → / ← rnpB pentapetide repeats‑related protein/RNase P, M1 RNA component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,270,1010CA 5.9% 1429.8 / 15.3 500intergenic (+499/+115)yhaC/rnpBpentapetide repeats‑related protein/RNase P, M1 RNA component
Reads supporting (aligned to +/- strand):  ref base C (244/197);  new base A (34/9);  total (288/212)
Fisher's exact test for biased strand distribution p-value = 3.22e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 514 total aligned reads displayed.

AAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTG  >  NC_000913/3269952‑3270250
                                                                                                                                                     |                                                                                                                                                     
aaaGTAACAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAc                                                                                                                                                       <  2:3380201/150‑1 (MQ=38)
aaaGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAc                                                                                                                                                       >  2:853916/1‑150 (MQ=38)
aaaGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTATCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAc                                                                                                                                                       <  2:10014901/150‑1 (MQ=38)
   gTAAAAACCCGCCGTAGCGGTTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAg                                                                                                                                                    <  1:6771476/150‑1 (MQ=255)
   gTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAg                                                                                                                                                    <  2:643891/150‑1 (MQ=38)
   gTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTAAGTAAACCAg                                                                                                                                                    <  2:8120117/150‑1 (MQ=38)
       aaaCCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGa                                                                                                                                                >  1:2970641/1‑150 (MQ=17)
         aCCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGaaa                                                                                                                                              >  1:10305752/1‑150 (MQ=17)
         aCCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGaaa                                                                                                                                              <  2:9203937/150‑1 (MQ=37)
         aCCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGaaa                                                                                                                                              <  1:2392943/150‑1 (MQ=37)
         aCCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAAACCGCCGAAGCGGGTTTTTACGTTAAGCAGGTGaaa                                                                                                                                              >  1:9158395/1‑150 (MQ=255)
          cccGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAAc                                                                                                                                             <  2:545066/150‑1 (MQ=37)
          cccGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTATGCCCCTCCAAAAGAAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAAc                                                                                                                                             >  1:6184723/1‑150 (MQ=255)
           ccgccgAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACt                                                                                                                                            <  2:407900/150‑1 (MQ=17)
           ccgccgAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACt                                                                                                                                            <  1:1328708/150‑1 (MQ=37)
              ccgAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTACACCAGGTGAAACTGAc                                                                                                                                         <  2:4163247/150‑1 (MQ=37)
              ccgAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTAGAGGGTCGTGGACAGTCATTCATCTTTATGCCCCTCCAAAATCAAAAACCCGCCGAAGCGGGTTTTTAAGTAAAACAGGTGGAACTGAc                                                                                                                                         >  2:3570378/1‑150 (MQ=11)
               cgAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGAcc                                                                                                                                        <  1:7482940/150‑1 (MQ=37)
                     gggTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAg                                                                                                                                  >  1:8085917/1‑150 (MQ=21)
                      ggTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTTGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGc                                                                                                                                 <  2:6637498/150‑1 (MQ=37)
                      ggTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGc                                                                                                                                 <  2:9506659/150‑1 (MQ=37)
                       gTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGcc                                                                                                                                <  2:1839813/150‑1 (MQ=17)
                       gTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGcc                                                                                                                                <  1:7335710/150‑1 (MQ=37)
                            tACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAACAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGcttt                                                                                                                           <  2:6952261/150‑1 (MQ=255)
                                 aaaaCAGGTGACACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCCGGTGAAACTGACCGATAAGCCGCTTTCtttt                                                                                                                      <  2:7548619/150‑1 (MQ=255)
                                 aaaaCAGGTCAAACTGACCGATAATCCGCTTTCTTTTGGGTATAGTGTAGTTGACACTCATAAATCTTTCTGCCCCTCCAAAAGAAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCtttt                                                                                                                      <  1:4618440/150‑1 (MQ=11)
                                  catcAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTg                                                                                                                     <  2:7686850/147‑1 (MQ=17)
                                  aaaCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTg                                                                                                                     <  2:10764719/150‑1 (MQ=39)
                                   aaCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTgg                                                                                                                    <  1:9348639/150‑1 (MQ=38)
                                    aCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTAAGTAAAACAGGTGAAACTGACCGGTAAGCCGCTTTCTTTTgtg                                                                                                                   >  1:10867709/1‑148 (MQ=255)
                                         tGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGc                                                                                                                              >  2:4430913/1‑134 (MQ=35)
                                         tGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGc                                                                                                                              <  1:4430913/134‑1 (MQ=35)
                                             aCTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACCGTCAGTCATCTTTCAGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCGTTCTTTTGGGGATAGcgtc                                                                                                          >  1:7642353/1‑150 (MQ=11)
                                             aCTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGcgtc                                                                                                          <  1:9501841/150‑1 (MQ=37)
                                               tGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGcgtcgt                                                                                                        >  2:1412226/1‑150 (MQ=37)
                                                  ccGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGa                                                                                                     <  1:1548518/150‑1 (MQ=37)
                                                   cGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGAc                                                                                                    <  2:7243789/150‑1 (MQ=37)
                                                   cGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTAGGGTAAAGCGACGTGGAc                                                                                                    >  2:4525955/1‑150 (MQ=255)
                                                     aTAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGTCAGTCATTCATATTTATGCCCCACCAAAAGCAAAAAACCGCCCAAGCGGGTTTTTAAGTAAAACAGGTGAAACTGACCGAAAAGCCGCATTATTTTGGGTATAGCGTCGTgggaag                                                                                                  >  1:6443102/1‑146 (MQ=11)
                                                     aTAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAg                                                                                                  >  2:2608307/1‑150 (MQ=37)
                                                     aTAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGAACGATAATCCGCTTTCTTTTGGGTATAGCGTCGTGGACAg                                                                                                  >  2:8603332/1‑150 (MQ=255)
                                                          ccGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTAAAGCGAATTGGAAAGtcaat                                                                                             >  2:7072144/1‑148 (MQ=11)
                                                            gCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTGTTGGGTATAGAGTATTGGACAGtcattca                                                                                           >  2:6099000/1‑150 (MQ=255)
                                                                tctttTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAAACCCCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTAAAGCGTCGTGGACACTAAATAATCtt                                                                                       >  2:1132941/1‑150 (MQ=11)
                                                                  ttttGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAATCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGAAAGTCATTCATCatta                                                                                     >  2:2492215/1‑149 (MQ=255)
                                                                  ttttGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGa                                                                                                                                          <  1:10701248/97‑1 (MQ=37)
                                                                  ttttGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGa                                                                                                                                          >  2:10701248/1‑97 (MQ=37)
                                                                   tttGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCGTTTGGGTATAGCGTCGTGGACAGTCAATAATCtttat                                                                                    >  2:5838438/1‑148 (MQ=255)
                                                                     tGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGc                                                                                  <  1:3944032/150‑1 (MQ=37)
                                                                        gTATAGTGTCGTGGACAGTCATTCATCTTTCTCCCCAACCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACAGACCGATAAGCCGCTTTCTTGTGGGTAAAGCGACGGGGAAAGGCCTTCATCGTTCTGcccc                                                                               >  2:9066694/1‑150 (MQ=11)
                                                                         tataGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTg                                                                                                                     <  1:5545922/111‑1 (MQ=35)
                                                                         tataGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGt                                                                                                 >  2:6126683/1‑131 (MQ=37)
                                                                           taGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTcc                                                                            <  2:7979797/150‑1 (MQ=37)
                                                                              tgtCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCaaa                                                                         >  2:2081088/1‑150 (MQ=37)
                                                                                tCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGCCAGGCATTCATCGTTCTGCCCCGCCAAAAg                                                                       >  2:10502579/1‑150 (MQ=255)
                                                                                  gTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCACCAAAAGCa                                                                     >  2:6850917/1‑150 (MQ=37)
                                                                                     gACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCaaaa                                                                  >  2:473540/1‑150 (MQ=37)
                                                                                      aCAGTCTTTGATCTTTCTGGCACTCAACAATCTTAAACCCGCCGAATCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCaaaaa                                                                 <  2:4919564/150‑1 (MQ=11)
                                                                                      aCAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTTCGTAAACCCGTTGAAACTGACCGATTAGACGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCaaaaa                                                                 <  1:589371/150‑1 (MQ=11)
                                                                                        aGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAAcc                                                               >  1:1400695/1‑150 (MQ=37)
                                                                                        aGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAAcc                                                               >  1:10015829/1‑150 (MQ=37)
                                                                                             ttcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAAAccgccg                                                          >  1:3739802/1‑150 (MQ=37)
                                                                                              tcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGa                                                         >  1:2633871/1‑150 (MQ=37)
                                                                                              tcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGa                                                         >  1:486425/1‑150 (MQ=37)
                                                                                              tcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTGTTGGGTATCGCGTCGTGGACAGTCATTCATCTGTCTGCCCCTCCAAAAGCAAAAACccgccgg                                                         >  1:7978556/1‑149 (MQ=255)
                                                                                               catCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAACCAAAAACCCGCCGaa                                                        >  1:1870271/1‑150 (MQ=37)
                                                                                                atCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAg                                                       >  2:9902456/1‑150 (MQ=37)
                                                                                                  cTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTATGCCCCGCCAAAAGCAAAAACCCGCCGCAGCg                                                     >  2:6757192/1‑150 (MQ=255)
                                                                                                   tttCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCgg                                                    <  1:7047944/150‑1 (MQ=37)
                                                                                                     tCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTAGAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCCAGGCGGGt                                                  >  1:1914477/1‑150 (MQ=255)
                                                                                                       tGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGttt                                                <  1:5945628/150‑1 (MQ=37)
                                                                                                         ccccTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGAt                                                                                                                                     >  2:10246082/1‑63 (MQ=35)
                                                                                                         ccccTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGcttt                                                                                                                           >  1:255318/1‑73 (MQ=40)
                                                                                                         ccccTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGttttt                                              >  2:3797158/1‑150 (MQ=37)
                                                                                                         ccccTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTACAAAATCAAAAAACCGCCGAAGCGGGttttt                                              >  2:510005/1‑150 (MQ=255)
                                                                                                            cTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTGAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACg                                           >  2:9795165/1‑150 (MQ=37)
                                                                                                            cTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTGTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACg                                           >  1:406641/1‑150 (MQ=37)
                                                                                                               cAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTaa                                        >  1:8891584/1‑150 (MQ=37)
                                                                                                                aaaaGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTaaa                                       >  1:8460807/1‑150 (MQ=37)
                                                                                                                 aaaGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAt                                      >  2:7904510/1‑150 (MQ=38)
                                                                                                                    gtaaaaaCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCATTCTGCCCCGCCAAAAGCAAAAAAACCCCGAAGCGGGGTTTTAAGTAAAACAg                                   >  1:3387962/3‑150 (MQ=11)
                                                                                                                     taaaaaCCCGCTTCGGCGGGTTTTTACGTAAACCAGGTGAAACAGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCCATCATATTTCTGCCCCAACAAAAGCCAAAACCACCCGCAGCGGGTTTTTAAGTaaaaaatg                                  >  1:10182301/2‑145 (MQ=11)
                                                                                                                     cAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATACGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAgg                                  <  2:1676669/150‑1 (MQ=38)
                                                                                                                      aaaaaCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGt                                 >  2:589343/1‑150 (MQ=37)
                                                                                                                       aaaaCCCGCTTCGGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAt                                      <  1:3593114/144‑1 (MQ=255)
                                                                                                                       aaaaCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCaaa                                                                   <  1:4814/115‑1 (MQ=35)
                                                                                                                         aaCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGaa                              >  1:6254192/1‑150 (MQ=37)
                                                                                                                          aCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGaaa                             >  2:9386139/1‑150 (MQ=37)
                                                                                                                             cgccgAAGCGGGTTTTTACGTAAACCCGGTGAAACGGACCGATAAGCCGCTTTCTTTTGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGa                         <  2:2956053/150‑1 (MQ=255)
                                                                                                                             cgccgAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTGTCTGCCCCGCCCAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTg                          >  2:8920261/1‑150 (MQ=255)
                                                                                                                                cgAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAAATGAcc                       >  1:2236197/1‑150 (MQ=37)
                                                                                                                                 gAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTGTTTACGTAAATCAGGTGAAAAAGACCg                      >  2:5049679/1‑150 (MQ=255)
                                                                                                                                       ggTTTTTTGGTAAATCCGCTGTAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGc                <  1:5188806/150‑1 (MQ=11)
                                                                                                                                       ggTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGc                <  1:5084498/150‑1 (MQ=37)
                                                                                                                                       ggTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGc                <  2:10861069/150‑1 (MQ=17)
                                                                                                                                          ttttACGTAAACCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCgg             <  1:9799276/150‑1 (MQ=38)
                                                                                                                                          ttttACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCgg             <  1:4283527/150‑1 (MQ=38)
                                                                                                                                            ttACGTAAACCAGGTGAAACTGACCGATCAGCGGCTTTCTTTTGTGTATAGCGTCGTGTACAGTAATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGTAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGt           <  1:3864826/150‑1 (MQ=14)
                                                                                                                                                gTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCAACTTTCTGCCCCGCCAAAAGCAAAAACCCCCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACAGACCGATAAGCCGGGTTCTg       >  1:2012408/1‑150 (MQ=255)
                                                                                                                                                  aaaCCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACa                                                                                                   <  2:3689692/56‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTG  >  NC_000913/3269952‑3270250

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: