Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,018,986 A→T 5.2% intergenic (+49/+27) matP → / ← ompA Ter macrodomain organizer matS‑binding protein/outer membrane protein A (3a;II*;G;d)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,018,9860AT 5.2% 857.9 / 19.7 249intergenic (+49/+27)matP/ompATer macrodomain organizer matS‑binding protein/outer membrane protein A (3a;II*;G;d)
Reads supporting (aligned to +/- strand):  ref base A (141/94);  new base T (7/7);  total (148/101)
Fisher's exact test for biased strand distribution p-value = 5.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 256 total aligned reads displayed.

GATTGTTCAGCTGATTGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGT  >  NC_000913/1018838‑1019135
                                                                                                                                                    |                                                                                                                                                     
   tGTTCAGCTGATTGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCgg                                                                                                                                                   <  2:2406625/150‑1 (MQ=255)
   tGTTCAGCTGATTGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCgg                                                                                                                                                   <  2:1730703/150‑1 (MQ=255)
    gTTCAGCTGATTGACGTTGCGGAAATCAAAGAGAAATCCGCGAATAATATGTCTTCTCTGAAGCAGGAACTGCAGGCATTGCTGGGTTAGGAATAACTGACGTATGTCAGTTGAATTTTCTAAAGGCAAAAAAAACCCCGCAGCTGCggg                                                                                                                                                  <  2:1623246/150‑1 (MQ=255)
    gTTCAGCTGATTGAAGATGCGTAAAACAAAGAGAAATACGCGAATAAAACGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAGAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCggg                                                                                                                                                  <  2:4011989/150‑1 (MQ=255)
    gTTCAGCTGATTGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCggg                                                                                                                                                  >  1:815048/1‑150 (MQ=255)
    gTTCAGCTGATTGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCggg                                                                                                                                                  <  2:1912776/150‑1 (MQ=255)
      tctgCTGATTTAATATGCGTAAAACAAAGATAAATACGCTAATAAAATGTCTTCTCTGAAGCTGGATCTGCTGGCTTTGGTGGTTAAGGAATAACTGACTAATTTATTTTAAATTTAATAAAGCAAAAAAAAACCCCGCAGCAGCGGGGt                                                                                                                                                <  1:837227/147‑1 (MQ=255)
        aGCTGATTGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGttt                                                                                                                                              <  2:2259228/150‑1 (MQ=255)
          cTGATTGAAGATGCGGAAAACAAATATAAATAAGCGAATAAAATTTCTTATCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTTACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGttttt                                                                                                                                            <  1:2177853/150‑1 (MQ=255)
          cTGATTGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGTAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGttttt                                                                                                                                            <  1:164958/150‑1 (MQ=255)
           tGATTTAATATGCGTAAAACAAAGAGAAATACGCGAATAAAATGTCTTCGCTGAAGCTGGATCTGCAGGCATTGCTGGGTACGGAATAACTGCCGAAAGTCAGTTCAATTTACTAATGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTc                                                                                                                                           <  1:3667690/150‑1 (MQ=255)
           tGATTGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCTGCTGCGGGGtttttt                                                                                                                                           <  2:88329/150‑2 (MQ=255)
            gATTGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCTGCTGCGGGGttttttt                                                                                                                                          <  2:1002210/150‑3 (MQ=255)
                          aaaaCAAAGAGAAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACt                                                                                                                            >  1:3195666/1‑150 (MQ=255)
                              cAAAGACAAATACGCGAATAAAATGTATTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAg                                                                                                                        <  1:1206390/150‑1 (MQ=255)
                               aaaGAGAAATACGCGAATAACATGTCTTCTCTGAAGCAGGATCTGCAGGCCTTGCTGGGTAAGGAATACCTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCATCGGGGTTTTTCTACCAGACGAGAACTTAAGc                                                                                                                       <  2:2645444/150‑1 (MQ=255)
                               aaaCAGACAGACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTACGGAATAACTGACGAACGTCAGTTCAATTTACTAAAGGCACAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGc                                                                                                                       <  2:3626627/150‑1 (MQ=255)
                                aaGAGAAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGcc                                                                                                                      >  1:552078/1‑150 (MQ=255)
                                  gagaAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTg                                                                                                                    >  2:2147406/1‑150 (MQ=255)
                                   agaAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAATAAATACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGc                                                                                                                   >  1:2501740/1‑150 (MQ=255)
                                     aaaTACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCgg                                                                                                                 >  1:2000217/1‑150 (MQ=255)
                                     aaaTACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTCCTACCAGACGAGAACTTAAGCCTGCgg                                                                                                                 >  1:1937272/1‑150 (MQ=255)
                                      aaTACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGc                                                                                                                >  2:797376/1‑150 (MQ=255)
                                       aTACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCt                                                                                                               <  2:3963365/150‑1 (MQ=255)
                                       aTACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCt                                                                                                               <  2:2003502/150‑1 (MQ=255)
                                         ccgcgAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGACTAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGa                                                                                                             <  1:2284090/149‑1 (MQ=255)
                                               aTAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACa                                                                                                       >  2:4096233/1‑150 (MQ=255)
                                               aTAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACa                                                                                                       <  2:2066001/150‑1 (MQ=255)
                                                 aacaTGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAACGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAAc                                                                                                     <  1:2126257/147‑1 (MQ=255)
                                                  aaaTGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACg                                                                                                    >  2:912056/1‑150 (MQ=255)
                                                  aaaTGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGCCTGAGTTACAACg                                                                                                    >  2:1123230/1‑150 (MQ=255)
                                                      gTCTTCTCTGAAGCAGGTTCTGCAGGCAGTGCTGGGTAAGGAATAACGGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCtt                                                                                                <  1:1745402/150‑1 (MQ=255)
                                                       tcttctCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCttt                                                                                               <  1:672425/150‑1 (MQ=255)
                                                       tcttctCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCttt                                                                                               <  1:2916322/150‑1 (MQ=255)
                                                       tcttctCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCttt                                                                                               <  1:2644423/150‑1 (MQ=255)
                                                       acttctCTGAAGCAGGATCTGCAGGCATTGCTGGGAAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTACTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCttt                                                                                               <  2:655012/149‑1 (MQ=255)
                                                         ttctCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGa                                                                                             >  2:2711662/1‑150 (MQ=255)
                                                            tctGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATAc                                                                                          >  1:279839/1‑150 (MQ=255)
                                                            tctGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATAc                                                                                          >  1:558017/1‑150 (MQ=255)
                                                            tctGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACAAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATAc                                                                                          >  1:2648425/1‑150 (MQ=255)
                                                             ctGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATAcc                                                                                         >  1:3048131/1‑150 (MQ=255)
                                                              tGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAACTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCt                                                                                        >  2:250599/1‑150 (MQ=255)
                                                               gAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCtt                                                                                       >  1:1513326/1‑150 (MQ=255)
                                                                  gCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTaa                                                                                    >  2:277514/1‑150 (MQ=255)
                                                                   cAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAAc                                                                                   >  1:2257017/1‑150 (MQ=255)
                                                                   cAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAAc                                                                                   >  1:3442906/1‑150 (MQ=255)
                                                                      gATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTc                                                                                >  2:3827072/1‑150 (MQ=255)
                                                                      gATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTc                                                                                >  1:1002398/1‑150 (MQ=255)
                                                                      gATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTc                                                                                >  2:3926900/1‑150 (MQ=255)
                                                                        tCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCATTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGATTTACAACGTCTTTTATACCTTTAACTTCGa                                                                              <  1:385777/150‑1 (MQ=255)
                                                                         cTGCAGGCATTGCTGTGTAATTAATAACTGACGAAATTCAGTTCATTTTACTAAAGTCAAAAAAAACCCCGCAGCAGGGGGTTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGAt                                                                             <  1:873995/150‑1 (MQ=255)
                                                                              ggCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTa                                                                        <  1:2376718/150‑1 (MQ=255)
                                                                               gCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTAc                                                                       >  1:3262268/1‑150 (MQ=255)
                                                                                      tGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAATCCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGCGTTACACCGTCTTTGATACCTTTAACGTCGATCTCTACGcgccga                                                                >  1:749543/1‑150 (MQ=255)
                                                                                       gggTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGAt                                                               >  1:3613832/1‑150 (MQ=255)
                                                                                           aaGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCgg                                                           <  1:3363553/150‑1 (MQ=255)
                                                                                                aTAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCa                                                      >  2:2389165/1‑150 (MQ=255)
                                                                                                     tGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAg                                                 >  2:2206675/1‑150 (MQ=255)
                                                                                                     tGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAg                                                 >  2:3820867/1‑150 (MQ=255)
                                                                                                     tGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAg                                                 >  1:2324280/1‑150 (MQ=255)
                                                                                                     tGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAg                                                 >  1:2761407/1‑150 (MQ=255)
                                                                                                     tGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAAACCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAATCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTATACGCGACGATCCGGAGCCAGGCAg                                                 >  2:3789437/1‑150 (MQ=255)
                                                                                                       aCGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTc                                               <  2:3468008/150‑1 (MQ=255)
                                                                                                         gAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGa                                             <  1:160917/150‑1 (MQ=255)
                                                                                                          aaaGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGAt                                            <  1:912056/150‑1 (MQ=255)
                                                                                                           aaGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATc                                           <  2:1541209/150‑1 (MQ=255)
                                                                                                           aaGTCAGTACAATTTACTAAAGGCAAAAAAATCCCCGCAGCAGCGGGTTTTTTCTACCATACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATc                                           <  2:3024010/150‑1 (MQ=255)
                                                                                                            aGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCa                                          <  1:3283447/150‑1 (MQ=255)
                                                                                                             gTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAg                                         >  2:945673/1‑150 (MQ=255)
                                                                                                              tCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGt                                        <  1:2025486/150‑1 (MQ=255)
                                                                                                                aGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTgc                                      <  1:2961780/150‑1 (MQ=255)
                                                                                                                 gTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTgca                                     <  2:2125260/150‑1 (MQ=255)
                                                                                                                  ttCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTgcag                                    >  1:4059118/1‑150 (MQ=255)
                                                                                                                  ttCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTgcag                                    >  2:1187229/1‑150 (MQ=255)
                                                                                                                       tttACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGc                               >  1:236601/1‑150 (MQ=255)
                                                                                                                         tACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTg                             <  1:2605515/150‑1 (MQ=255)
                                                                                                                            tAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCCGACGAGACCTTAAGCCTGCGGCTGAGGTACAACGTCTTTGATACCGTTAACTTCGATCTCTACGCGCCGATCCGGAGCCAGGCAGTCGAGCAGTGCGGCACGCTGttg                          >  1:1300752/1‑149 (MQ=255)
                                                                                                                            tAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCa                                                  >  2:1525535/1‑126 (MQ=255)
                                                                                                                            tAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGttt                          >  2:2587789/1‑150 (MQ=255)
                                                                                                                              aaGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCa                        >  2:3691945/1‑150 (MQ=255)
                                                                                                                              aaGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCa                        >  1:2693978/1‑150 (MQ=255)
                                                                                                                                 gCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGt                     >  1:1123271/1‑150 (MQ=255)
                                                                                                                                  cAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGTTCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGtt                    <  1:1851557/150‑1 (MQ=255)
                                                                                                                                  cAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGtt                    >  1:3750053/1‑150 (MQ=255)
                                                                                                                                  cAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGtt                    >  2:1282545/1‑150 (MQ=255)
                                                                                                                                   aaaaaaaaCCCCGCAGCAGCGGGGTTTTTCTACCCGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTg                   >  1:3785498/1‑150 (MQ=255)
                                                                                                                                   aaaaaaaaCCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTg                   >  1:1617134/1‑150 (MQ=255)
                                                                                                                                   aaaaaaaaCCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTg                   >  2:3770012/1‑150 (MQ=255)
                                                                                                                                     aaaaaaCCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCCGTCGATCAGTGCAGCACGCTGTTTCACGTTGTc                 >  2:2945610/1‑150 (MQ=255)
                                                                                                                                       aaaaCCCCGCTGCTGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCCGTCGAGCAGTGCAGCACGCTGTTTCACGTTGTcac               >  1:3577537/1‑150 (MQ=255)
                                                                                                                                        aaaCCCCGCTGCTGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTcaca              >  1:734137/1‑150 (MQ=255)
                                                                                                                                        aaaCCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTcaca              <  2:3127215/150‑1 (MQ=255)
                                                                                                                                         aaCCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAg             <  2:558017/150‑1 (MQ=255)
                                                                                                                                         aaCCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCGGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAg             >  1:1508004/1‑150 (MQ=255)
                                                                                                                                           ccccGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGgt           <  1:1394222/150‑1 (MQ=255)
                                                                                                                                            cccGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGgtg          >  2:3319073/1‑150 (MQ=255)
                                                                                                                                            cccGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGgtg          >  2:10480/1‑150 (MQ=255)
                                                                                                                                            cccGCAGCAGCGGGGTTATTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGgtg          >  1:3408350/1‑150 (MQ=255)
                                                                                                                                              cGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGtt        >  1:1071666/1‑150 (MQ=255)
                                                                                                                                                    agcGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGt  <  1:1126153/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GATTGTTCAGCTGATTGAAGATGCGGAAAACAAAGAGAAATACGCGAATAAAATGTCTTCTCTGAAGCAGGATCTGCAGGCATTGCTGGGTAAGGAATAACTGACGAAAGTCAGTTCAATTTACTAAAGGCAAAAAAAACCCCGCAGCAGCGGGGTTTTTCTACCAGACGAGAACTTAAGCCTGCGGCTGAGTTACAACGTCTTTGATACCTTTAACTTCGATCTCTACGCGACGATCCGGAGCCAGGCAGTCGATCAGTGCAGCACGCTGTTTCACGTTGTCACAGGTGTTGCCAGT  >  NC_000913/1018838‑1019135

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: