Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,287,149 G→A 7.2% noncoding (88/171 nt)
R10* (CGA→TGA) 
rttR ←
tpr ←
rtT sRNA, processed from tyrT transcript
protamine‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,287,1490GA 7.2% 388.8 / 15.8 139noncoding (88/171 nt)
R10* (CGA→TGA) 
rttR
tpr
rtT sRNA, processed from tyrT transcript
protamine‑like protein
Reads supporting (aligned to +/- strand):  ref base G (62/67);  new base A (5/5);  total (67/72)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01

Only 100 of 139 total aligned reads displayed.

TTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGT  >  NC_000913/1287001‑1287297
                                                                                                                                                    |                                                                                                                                                     
ttCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCACTCTCGCTTGCGCGCGa                                                                                                                                                      >  1:125998/1‑150 (MQ=255)
ttCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCGGCGGCACCGCTCTCTCGCTGGCGCTCGa                                                                                                                                                      >  1:1512849/1‑150 (MQ=255)
ttCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTGGCCTGCGGCAACGCTATCTCGCTGGCGCTCGa                                                                                                                                                      >  1:358636/1‑150 (MQ=255)
 tCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGAGCTTCGCCCTTCGGGTCGTGGCCGGACGCAAAGCACTCTCGCTGGCGCGCGaa                                                                                                                                                     >  2:1882957/1‑149 (MQ=14)
  ccccGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGt                                                                                                                                                    <  1:2370586/150‑1 (MQ=255)
   cccGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGGGGCACCGCGCTCTCGCTGTCGCTCGAGTc                                                                                                                                                   >  1:743042/1‑150 (MQ=255)
        aTGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTTCCTGCGGCAACCCTCTCTCGCTGGCGCACGAGTCGAAcc                                                                                                                                              >  1:1669410/1‑150 (MQ=255)
        aTGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGGGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCACACACGCAGGCGCACGAGTCGaaac                                                                                                                                              >  1:1474028/1‑148 (MQ=14)
          gggCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCtt                                                                                                                                            <  1:916564/150‑1 (MQ=255)
             cAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGt                                                                                                                                         >  2:1303626/1‑150 (MQ=255)
             cAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGt                                                                                                                                         >  1:2714262/1‑150 (MQ=255)
              aGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTAGCTGGCGCTCGAGTCGAACCTTGGTc                                                                                                                                        >  1:2621958/1‑150 (MQ=255)
               gAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTTCCTGAGGCAACGCTCTCTCGCTTGCGCTCGAGTCGAACCTTGGTCg                                                                                                                                       >  2:2537234/1‑150 (MQ=255)
               gAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGTCGCTCGAGTCGAACCGTTGTCg                                                                                                                                       >  1:453923/1‑150 (MQ=255)
               gAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCg                                                                                                                                       <  1:1515118/150‑1 (MQ=255)
               gAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCg                                                                                                                                       >  2:2537231/1‑150 (MQ=255)
               gAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTACGCCCTTCGGGTCGTTGCCGGGGGCAACGCTCTCACGCTGGCCCTCGAGTCGAACCTTGGTCg                                                                                                                                       >  2:460496/1‑150 (MQ=14)
                 atatTTGACTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGaa                                                                                                                                     <  1:678632/150‑1 (MQ=255)
                  tatTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAg                                                                                                                                    >  2:3375799/1‑150 (MQ=255)
                                     ttGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTTCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCa                                                                                                                 >  2:4088483/1‑150 (MQ=255)
                                     ttGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCAGGCGCTCGAGTCGAACCTTTGTCGAAGCTTCTCAACCTTACCCGct                                                                                                                 >  1:1616287/1‑149 (MQ=255)
                                            ttCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCAAGCTGGCGCTCGAGTCGAACCATGGTCGAAGCTTATCAAACATACCCCCACGGGCAg                                                                                                          >  1:2544217/1‑150 (MQ=14)
                                             tCAGGGACTTTGGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCCACGCGCACTCGCTGGCGCACGAGTCGAACCGTGGTCGCATCTAGACATCCTTCCCCGCATGGGGAgg                                                                                                         >  1:1298195/1‑149 (MQ=14)
                                                     ttttGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTTCCTGCGGCAACGCTCTCTCGCGGGCGCTCGGGTCGAACCGTGGTCGAAGCGTCACATCCTTCCCCCCATGGGCAGGATATTTag                                                                                                 >  1:3028383/1‑149 (MQ=11)
                                                     ttttGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGAGGCAACGCTATCACGCTTGCGCTCGATTCGAACCATGGTCGAATCTTCTCAAACATCCCCGCATGGGCCGAAGATTTaa                                                                                                 >  1:193016/1‑150 (MQ=11)
                                                       ttGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCAGCGGCAACCCTCACACCCTGGCGCGCGAGTCGAACCCTGGTCGAAGCTTCTCATCCTACCCCCCCTGGGCCGAATATTTAAtt                                                                                               >  1:908763/1‑150 (MQ=14)
                                                         gaaatataTGGTGGTGGCGGAAGCATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGc                                                                                             <  1:3404616/143‑1 (MQ=255)
                                                         gAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGc                                                                                             <  1:1004224/150‑1 (MQ=39)
                                                          aaaGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTccc                                                                                                                     >  2:2855307/1‑125 (MQ=35)
                                                          aaaGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTccc                                                                                                                     <  1:2855307/125‑1 (MQ=35)
                                                           aaGTGATGGTGGTGGGGGAAGGATTACACAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGAGGCAACGCTCTCTCGCTGCCGCACGAGGCGAACCTTCGTCGAAGCATCTCATCCATCCCCGCATGGGCCGAATATTTAAtagagg                                                                                           >  2:3043847/1‑145 (MQ=11)
                                                               gATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGTCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTc                                                                                       <  1:1077536/150‑1 (MQ=37)
                                                                    tggtggGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTgg                                                                                 <  1:3375799/150‑1 (MQ=38)
                                                                           ggAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGaa                                                                                                                                                <  1:898267/81‑1 (MQ=33)
                                                                           ggAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGaa                                                                                                                                                >  2:3539862/1‑81 (MQ=33)
                                                                           ggAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGaa                                                                                                                                                >  2:898205/1‑81 (MQ=33)
                                                                           ggAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGaa                                                                                                                                                <  1:3539862/81‑1 (MQ=33)
                                                                           ggAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGaa                                                                                                                                                >  2:898267/1‑81 (MQ=33)
                                                                           ggAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTc                                                                          <  1:3208459/150‑1 (MQ=255)
                                                                           ggAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTAATCCATCCCCGCATGGGCAGAATATTTAATTGCGGATTCGGTGGGAAGGTc                                                                          >  2:1166634/1‑150 (MQ=255)
                                                                           ggAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCGCTCGCTGGCGCTCGACTCGAACCTTGGTCGAAGCGTCTCAACCTTCCCCGCATGGGCAGACTATTTAATTTCGGATTCGTTGGGCAGTTc                                                                          >  2:1166643/1‑150 (MQ=14)
                                                                           ggAAGGAGTACTCAGCGCTGCGCGCTTCGGCCTTCGGGTCGGTGCCTGCGGCACCGCTCTCTCGCTGGCGCTCGAGTCGAACCTGGGTCGTAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTc                                                                          <  2:3272373/150‑1 (MQ=14)
                                                                            gAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCg                                                                                                                                                  <  1:307287/78‑1 (MQ=34)
                                                                            gAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCa                                                                         <  1:2377481/150‑1 (MQ=255)
                                                                            gAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCa                                                                         <  1:839324/150‑1 (MQ=255)
                                                                             aaGGATTACTCAGCGCTGCGCGCTTCGCCCTTCTGTTCTTTGGCTGCGCCATAGATCTAACGCTGGCGCTAGAGTCTTTCCTTTGTCGAAGCTTCTCATCCTTCCCGGCTTGGGATTAATATTTATTTGGGTTTCGTTTGTGAAGTTCAg                                                                        <  2:703653/150‑1 (MQ=2)
                                                                             aaGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAg                                                                        <  1:1012586/150‑1 (MQ=255)
                                                                                gATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGTCGCTCGAGTCGAACCTTTGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTTATTGCGGATTCGTTGGTAAGTTCAGGGa                                                                     <  1:1574520/150‑1 (MQ=255)
                                                                                gATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGa                                                                     <  1:432211/150‑1 (MQ=255)
                                                                                   tACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACtt                                                                  >  2:3867831/1‑150 (MQ=255)
                                                                                    aCTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGTCGCTCGAGGCGAACCTTTGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTTATTGCGGATTCGTTGGGAAGTTCAGGGACttt                                                                 <  1:1300137/150‑1 (MQ=255)
                                                                                     cTCGGCGCTGCGCGCTACGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACtttt                                                                <  1:3234450/150‑1 (MQ=255)
                                                                                     cTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGt                                                                                                                                         >  2:2525718/1‑78 (MQ=34)
                                                                                      tCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGTCGCTCGAGGCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTg                                                               <  1:3648287/150‑1 (MQ=255)
                                                                                      tCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCCGAATAGTTAATTGCGGATTCGTTGGGAAGGTCAGGGAcggttg                                                               >  2:2731424/1‑145 (MQ=255)
                                                                                      tCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTg                                                               <  1:3648220/150‑1 (MQ=255)
                                                                                      tCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTg                                                               <  1:1608234/150‑1 (MQ=255)
                                                                                      tCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTg                                                               <  2:2410692/150‑1 (MQ=255)
                                                                                      gcAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGTCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTg                                                               <  2:1526210/149‑1 (MQ=255)
                                                                                        aGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGaa                                                             <  1:2129608/150‑1 (MQ=255)
                                                                                          cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCGGCGGCAACGCTCGCTCGCGGGCGCACGAGTCGAACCGTGGTCCAAGCTTATCATCCGTCCCCGCATGGGCAGCAGATTTAATTGCGGATTCGTTGGGAAGGTCAGGGACATTTGCAAg                                                           >  2:658752/1‑150 (MQ=17)
                                                                                          cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCGGCGGCAACGCGCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCGTCTCATCCTTCCCCGCAGGGGCAGCCTATTTAAGTGCGGAGTCGGTGGGCAGTTCAGGGACTTTTGTAAg                                                           >  2:3881818/1‑150 (MQ=14)
                                                                                            cTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCACGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCCCAGGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACGTTTGAAAGTg                                                         >  2:1464159/1‑150 (MQ=255)
                                                                                               cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCtt                                                                                                                                            >  2:3039277/1‑65 (MQ=35)
                                                                                               cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTGGGTCGAAGCTTCTCAACCTTCCCCGCAGGGGCCGAACATTGAAATTCGGAAGCGTTGTGACCGTACGGGGCTTTTGAAAgtggtg                                                      >  2:2642243/1‑150 (MQ=11)
                                                                                                        cccTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGTCGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTgggg                                             <  1:671064/150‑1 (MQ=255)
                                                                                                        cccTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCGTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTg                                                                                              >  2:4111715/1‑102 (MQ=12)
                                                                                                         ccTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCGGGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAAAATTTCATTGCGGAGTCGGTGGGCAGTTCAGGGACTTTGGAAAGGGTTGgggggggggg                                            >  2:293020/1‑150 (MQ=14)
                                                                                                          cTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAg                                                                                                                                    <  1:4086435/62‑1 (MQ=35)
                                                                                                          cTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAg                                                                                                                                    >  2:4086435/1‑62 (MQ=35)
                                                                                                          cTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCa                                                                                                                             >  2:2077902/1‑69 (MQ=35)
                                                                                                          cTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCa                                                                                                                             <  1:2077902/69‑1 (MQ=35)
                                                                                                            tCGGGTCGTTGCCTGCGGCACCGCTCTCTCGCTGTCGCCCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAg                                         <  1:2973002/150‑1 (MQ=255)
                                                                                                              gggTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTTGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGa                                       >  2:3733493/1‑150 (MQ=255)
                                                                                                              gggTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCATACCCCCATGGGCAGAATATTTAAATGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGa                                       >  2:3733705/1‑150 (MQ=255)
                                                                                                                gTCGTTGCCTGCGGCAACGCTCTCTCGCTTGCCCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCCTGGGCCGAATATTTAATTGCGGATTCGGTGGGAAGTTCAGGGACTTTTGAAAGTGTTGGGGGTGGGGGAAGGAtt                                     >  2:572556/1‑150 (MQ=14)
                                                                                                                 tCGTTGCCTGCGGCAACGCTCTCTCGCTGTCGCTCGAGCCGAACCTTCGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTCATTGCGGATTCGTTGGTCAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTc                                    <  2:2389788/150‑1 (MQ=11)
                                                                                                                 tCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGaaa                                                            >  2:2191068/1‑126 (MQ=255)
                                                                                                                 tCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGaaa                                                            <  1:2191068/126‑1 (MQ=255)
                                                                                                                 tCGTTGCCTGCGGCAACGCTCCCTCGGTGTCGCTCGAGTCGAACCTCCGTCGTAGCGTCCCAGCCTTACCCGCTTTGTCAGAATATTTCATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTc                                    <  2:2389747/150‑1 (MQ=14)
                                                                                                                    ttGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGaa                                 >  2:195942/1‑150 (MQ=255)
                                                                                                                    ttGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTATCATCCATACCCGCAAGGGCAGAATATTTAATTGCGGATTCGTTGGGTAGTTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGTAGGATTAGaa                                 >  1:3703592/1‑150 (MQ=14)
                                                                                                                     tGCCTGCGGCAACGCTCTCTCGCTGTCGCTCGAGCCGAACCTTTGTCGAAGCTTCTCATCCGTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAAc                                <  1:2081436/150‑1 (MQ=255)
                                                                                                                      gCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAAcc                               >  2:2115406/1‑150 (MQ=255)
                                                                                                                        cTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCtt                             <  1:1603843/150‑1 (MQ=255)
                                                                                                                         tGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGACCCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTc                            <  2:2683264/150‑1 (MQ=255)
                                                                                                                         tGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTc                            <  1:2010738/150‑1 (MQ=255)
                                                                                                                           cGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGa                          <  2:2544217/150‑1 (MQ=255)
                                                                                                                           cGGCAACGCTCTCTCGCTGGCGCTCGAGCCGAACCTTGGTCGAAGCTTCTCATCTTTCGACGGTTGGTCAGAATTTTTAATTTCGGATTCGTTTGTTATTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGa                          <  1:217967/150‑1 (MQ=14)
                                                                                                                                  gCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGtt                                                                                   <  1:1845024/86‑1 (MQ=18)
                                                                                                                                  gCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGtt                                                                                   >  2:1845024/1‑86 (MQ=18)
                                                                                                                                   ctctctCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATg                  >  2:303294/1‑150 (MQ=255)
                                                                                                                                      tctcGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACg               >  2:601129/1‑150 (MQ=255)
                                                                                                                                         cGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGTAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCa            <  1:303294/150‑1 (MQ=255)
                                                                                                                                          gCTGGCGCTCGGGTCGAACCTTGGTCGAAGCTTCTCATCCGTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAg           <  1:3178471/150‑1 (MQ=255)
                                                                                                                                          gCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAg           <  1:3735236/150‑1 (MQ=255)
                                                                                                                                            tGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGAt         <  1:1450853/150‑1 (MQ=255)
                                                                                                                                              gcgcTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAgg                                                                       <  1:706391/86‑1 (MQ=255)
                                                                                                                                                gcTCGAGTCGAACCTTTGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAc     <  1:26586/150‑1 (MQ=255)
                                                                                                                                                 cTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACa    <  1:1492351/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
TTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGT  >  NC_000913/1287001‑1287297

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: