Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,388,285 A→G 5.2% intergenic (+24/+20) tyrR → / ← tpx aromatic amino acid biosynthesis and transport regulon transcriptional regulator; autorepressor; ATPase; phosphatase/lipid hydroperoxide peroxidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,388,2850AG 5.2% 718.3 / 19.2 212intergenic (+24/+20)tyrR/tpxaromatic amino acid biosynthesis and transport regulon transcriptional regulator; autorepressor; ATPase; phosphatase/lipid hydroperoxide peroxidase
Reads supporting (aligned to +/- strand):  ref base A (104/96);  new base G (5/6);  total (109/103)
Fisher's exact test for biased strand distribution p-value = 7.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.89e-01

Only 100 of 219 total aligned reads displayed.

TTAACCCAGCTTTATCGCAATTATCCCAGCACGCGCAAACTGGCAAAACGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATCCACCAGCTGGCTGAAAATCACATTGTCATTTTCGTCAATAACCACAACGGCACGCGCTGCCAGACCTTTCAGT  >  NC_000913/1388136‑1388434
                                                                                                                                                     |                                                                                                                                                     
ttAACCCAGCTTTATCGCAATTATCCCAGCACGCGCAAACTGGCAAAACGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCaa                                                                                                                                                       <  1:1545523/150‑1 (MQ=255)
 tAACCCAGCTTTATCGCAATTATCCCAGCACGCGCAAACTGGCAAAACGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAac                                                                                                                                                      <  2:2977050/150‑1 (MQ=255)
  aaCCCAGCTTTATCGCAATTATCCCAGCACGCGCAAACTGGCAAAACGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAaca                                                                                                                                                     >  2:4071308/1‑150 (MQ=255)
  aaCCCAGCTTTATCGCAATTATCCCAGCACGCGCAAACTGGCAAAACGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAaca                                                                                                                                                     >  2:1809122/1‑150 (MQ=255)
   aCCCAGCTTTATCGCAATTATCCCAGCACGCGCAAACTGGCAAAACGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAacac                                                                                                                                                    >  2:2087411/1‑150 (MQ=255)
     ccAGCTTTATCGCAATTATCCCAGCACGCGCAAACTGGCAAAACGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTACGCGCGACTATGCCTGATGGTGCAACACCa                                                                                                                                                  >  2:2816085/1‑150 (MQ=255)
     ccAGCTTTATCGCAATTATCCCAGCACGCGCAAACTGGCAAAACGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCa                                                                                                                                                  >  1:3710886/1‑150 (MQ=255)
     ccAGCTTTATCGCAATTATCCCAGCACGCGCAAACTGGCAAAACGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCa                                                                                                                                                  >  2:3747256/1‑150 (MQ=255)
     ccAGCTTTATCGCAATTATCCCAGCACGCGCAAACTGGCAAAACGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCa                                                                                                                                                  >  1:525419/1‑150 (MQ=255)
      cAGCTTTATCGCAATTATCCCAGCACGCGCAAACTGGCAAAACGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCAt                                                                                                                                                 >  1:345897/1‑150 (MQ=255)
        gCTTTATCGCAATTATCCCAGCACGCGCAAACTGGCAAAACGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCa                                                                                                                                               <  1:2583530/150‑1 (MQ=255)
        gCTTTATCGCAATTATCCCAGCACGCGCAAACTGGCAAAACGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCa                                                                                                                                               <  1:3488162/150‑1 (MQ=255)
         cTTTATCGCAATTATCCCAGCACGCGCAAACTGGCAAAACGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGTTGCACCATCAg                                                                                                                                              <  1:3478879/150‑1 (MQ=255)
         cTTTATCGCAATTATCCCAGCACGCGCAAACTGGCAAAACGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGTTGCACCATCAg                                                                                                                                              <  1:2356501/150‑1 (MQ=255)
         cTTTATCGCAATTATCCCAGCACGCGCAAACTGGCAAAACGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAg                                                                                                                                              <  1:3061807/150‑1 (MQ=255)
          tttATCGCAATTATCCCAGCACGCGCAAACTGGCAAAACGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGTCGCACCATCAgg                                                                                                                                             <  2:1332295/150‑1 (MQ=255)
          tttATCGCAATTATCCCAGCACGCGCAAACTGGCAAAACGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAgg                                                                                                                                             >  1:3243186/1‑150 (MQ=255)
                   aTTATCCCAGCACGCGCAAACTGGCAAAACGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTaaa                                                                                                                                    <  1:2357219/150‑1 (MQ=255)
                    ttATCCCAGCACGCGCAAACTGGCAAAACGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGACTATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAAt                                                                                                                                   >  2:3629317/1‑150 (MQ=255)
                    ttATCCCAGCACGCGCAAACTGGCAAAACGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAAt                                                                                                                                   >  2:3277874/1‑150 (MQ=255)
                    ttATCCCAGCACGCGCAAACTGGCAAAACGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAAt                                                                                                                                   >  1:2804194/1‑150 (MQ=255)
                       tCCCAGCACGCGCAAACTGGCAAAACGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGACGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATCTTaaaggag                                                                                                                                >  2:2792693/1‑146 (MQ=255)
                        cccAGCACGCGCAAACTGGCAAAACGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATg                                                                                                                               <  2:3673853/150‑1 (MQ=255)
                          cAGCACGCGCAAACTGGCAAAACGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGACTATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCCTATTAAATTATGCt                                                                                                                             >  1:1840849/1‑150 (MQ=255)
                          cAGCACGCGCAAACTGGCAAAACGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCt                                                                                                                             >  2:1448432/1‑150 (MQ=255)
                           aGCACGCGCAAACTGGCAAAACGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCtt                                                                                                                            <  2:2569779/150‑1 (MQ=255)
                              aCGCGCAAACTGGCAAAACGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCa                                                                                                                         <  2:3059217/150‑1 (MQ=255)
                               cgcgCAAACTGGCAAAACGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAg                                                                                                                        <  1:3334582/150‑1 (MQ=255)
                               cgcgCAAACTGGCAAAACGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAg                                                                                                                        <  1:3476661/150‑1 (MQ=255)
                                   cAAACTGGCAAAACGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACa                                                                                                                    <  1:993718/150‑1 (MQ=255)
                                     aaCTGGCAAAACGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGc                                                                                                                  <  2:3233759/150‑1 (MQ=255)
                                     aaCTGGCAAAACGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGc                                                                                                                  <  1:3139123/150‑1 (MQ=255)
                                     aaCTGGCAAAACGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGc                                                                                                                  <  1:1078562/150‑1 (MQ=255)
                                        tGGCAAAACGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCag                                                                                                               >  1:2215775/1‑150 (MQ=255)
                                          gCAAAACGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCagag                                                                                                             >  2:1766666/1‑150 (MQ=255)
                                            aaaaCGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAgct                                                                                                           >  2:3393714/1‑150 (MQ=255)
                                             aaaCGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAgctg                                                                                                          <  1:2032627/150‑1 (MQ=255)
                                                 gTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTc                                                                                                      <  1:1018449/150‑1 (MQ=255)
                                                 gTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTc                                                                                                      <  1:2997704/150‑1 (MQ=255)
                                                  tctcGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCg                                                                                                     <  1:935569/150‑1 (MQ=255)
                                                     cGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTaa                                                                                                  <  1:2682728/150‑1 (MQ=255)
                                                      ggCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAAt                                                                                                 >  2:1362511/1‑150 (MQ=255)
                                                       gCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATc                                                                                                <  1:389092/150‑1 (MQ=255)
                                                         gTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCg                                                                                              <  2:2036173/150‑1 (MQ=255)
                                                          tttCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCgg                                                                                             <  1:3666273/150‑1 (MQ=255)
                                                          tttCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCgg                                                                                             >  1:840639/1‑150 (MQ=255)
                                                            tCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCt                                                                                           >  1:3101199/1‑150 (MQ=255)
                                                              acaTACCTCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCg                                                                                         <  2:1171891/150‑1 (MQ=255)
                                                                  aCCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCGTATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCggtgg                                                                                     >  2:637416/1‑150 (MQ=255)
                                                                  aCCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCggtgg                                                                                     >  2:1067018/1‑150 (MQ=255)
                                                                   ccGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCggtggt                                                                                    >  1:3305515/1‑150 (MQ=255)
                                                                   ccGCGAGTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTCTGCTTTCAGTTCAGCCAGAGCTGCTTCGTAATCCGGCTCggtggt                                                                                    <  1:2319459/150‑1 (MQ=255)
                                                                    cgcgATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGgtggtg                                                                                   >  1:2871531/1‑150 (MQ=255)
                                                                    cgcgATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGgtggtg                                                                                   >  2:801076/1‑150 (MQ=255)
                                                                           gCCAATACGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCAt                                                                            <  1:2966070/150‑1 (MQ=255)
                                                                           gCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCAt                                                                            <  1:2763388/150‑1 (MQ=255)
                                                                             cAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATcc                                                                          >  2:2367335/1‑150 (MQ=255)
                                                                             cAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATcc                                                                          >  2:312055/1‑150 (MQ=255)
                                                                              aaTAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATcca                                                                         >  1:3255793/1‑150 (MQ=255)
                                                                              aaTAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATcca                                                                         >  2:559842/1‑150 (MQ=255)
                                                                                 aaGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATccacca                                                                      <  1:503550/150‑1 (MQ=255)
                                                                                   ggtGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTTAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTCAATTATGCTTTCTGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGGGGTGATTTCCTCACCCAgc                                                                    >  2:3131616/3‑150 (MQ=255)
                                                                                    ttGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATCCACCAgct                                                                   <  2:3271269/150‑1 (MQ=255)
                                                                                      gCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATCCACCAgctgg                                                                 <  2:3751463/150‑1 (MQ=255)
                                                                                      gCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATCCACCAgctgg                                                                 <  2:2505665/150‑1 (MQ=255)
                                                                                      gCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATCCACCAgctgg                                                                 <  1:1766666/150‑1 (MQ=255)
                                                                                        gggAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATCCACCAgctggct                                                               <  1:2794424/150‑1 (MQ=255)
                                                                                             tatGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATCCACCAGCTGGCTGaaaa                                                          <  2:2253229/150‑1 (MQ=255)
                                                                                                ggTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATCCACCAGCTGGCTGAAAATca                                                       <  1:2087411/150‑1 (MQ=255)
                                                                                                 gTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATCCACCAGCTGGCTGAAAATcac                                                      >  2:440172/1‑150 (MQ=255)
                                                                                                  tCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATCCCCCAGCTGGCTGAAAATcaca                                                     >  1:1238954/1‑150 (MQ=255)
                                                                                                  tCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATCCACCAGCTGGCTGAAAATcaca                                                     >  1:4008351/1‑150 (MQ=255)
                                                                                                  tCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATCCACCAGCTGGCTGAAAATcaca                                                     >  2:1614980/1‑150 (MQ=255)
                                                                                                    tGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATCCACCAGCTGGCTGAAAATCACAtt                                                   <  1:68740/150‑1 (MQ=255)
                                                                                                    tGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATCCACCAGCTGGCTGAAAATCACAtt                                                   <  1:3744818/150‑1 (MQ=255)
                                                                                                          agaagaagaACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATCCACCAGCTGGCTGAAAATCACATTGTCAtt                                             >  1:3200179/1‑150 (MQ=255)
                                                                                                             agtagaaCGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCTTATTAATTTATGCTTTCAGTACAGCCAGAGCTTCTTGGTAATCCGGCTGGGTGGTGATTTCATCCACCAGCTGGCTGAAAATCTCATTGTAATTTTc                                          <  2:390639/147‑1 (MQ=255)
                                                                                                                  aaCGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATCCACCAGCTGGCTGAAAATCACATTGTCATTTTCGTCaa                                     >  2:871564/1‑150 (MQ=255)
                                                                                                                  aaCGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATCCACCAGCTGGCTGAAAATCACATTGTCATTTTCGTCaa                                     >  2:3550327/1‑150 (MQ=255)
                                                                                                                  aaCGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATCCACCAGCTGGCTGAAAATCACATTGTCATTTTCGTCaa                                     <  2:658695/150‑1 (MQ=255)
                                                                                                                    cGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATCCACCAGCTGGCTGAAAATCACATTGTCATTTTCGTCAATa                                   >  1:1106051/1‑150 (MQ=255)
                                                                                                                    cGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATCCACCAGCTGGCTGAAAATCACATTGTCATTTTCGTCAATa                                   <  2:2746839/150‑1 (MQ=255)
                                                                                                                    cGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATCCACCAGCTGGCTGAAAATCACATTGTCATTTTCGTCAATa                                   >  1:3985084/1‑150 (MQ=255)
                                                                                                                      aaGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTc                                                                                                      <  1:1523408/81‑1 (MQ=255)
                                                                                                                      aaGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTc                                                                                                      >  2:1523518/1‑81 (MQ=255)
                                                                                                                               cgcgAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCCGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATACACCAGCTGGCTGAAAATCACATTGTAATTTTCGTCAAAAACCACAACGGc                        >  1:1503105/1‑150 (MQ=255)
                                                                                                                                gcgAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATCCACCAGCTGGCTGAAAATCACATTGTCATTTTCGTCAATAACCACAACGGCa                       >  1:261531/1‑150 (MQ=255)
                                                                                                                                         ccTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGgtggtg                                                                                   <  2:713391/81‑1 (MQ=255)
                                                                                                                                          cTGATGGTGTTGCACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATCCACCAGCTGGCTGAAAATCACATTGTCATTTTCGTCAATAACCACAACGGCACGCGCTGCCa             >  2:1290248/1‑150 (MQ=255)
                                                                                                                                          cTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATCCACCAGCTGGCTGAAAATCACATTGTCATTTTCGTCAATAACCACAACGGCACGCGCTGCCa             >  2:1492499/1‑150 (MQ=255)
                                                                                                                                          cTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATCCACCAGCTGGCTGAAAATCACATTGTCATTTTCGTCAATAACCACAACGGCACGCGCTGCCa             >  2:2404549/1‑150 (MQ=255)
                                                                                                                                           tGATGGTGTTGCACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATCCACCAGCTGGCTGAAAATCACATTGTCATTTTCGTCAATAACCACAACGGCACGCGCTGCCAg            >  1:1335732/1‑150 (MQ=255)
                                                                                                                                            gATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATCCACCAGCTGGCTGAAAATCACATTGTCATTTTCGTCAATAACCACAACGGCACGCGCTGCCAGa           <  1:4071308/150‑1 (MQ=255)
                                                                                                                                            gATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATCCACCAGCTGGCTGAAAATCACATTGTCATTTTCGTCAATAACCACAACGGCACGCGCTGCCAGa           >  2:4004434/1‑150 (MQ=255)
                                                                                                                                              tGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATCCACCAGCTGGCTGAAACTCACATTGTCATTTTCGTCAATAACCACAACGGCACGCGCTGCCAGAcc         >  1:1012347/1‑150 (MQ=255)
                                                                                                                                                gTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATCCACCAGCTGGCTGAAAATCACATTGTCATTTTCGTCAATAACCACAACGGCACGCGCTGCCAGACCtt       <  1:286823/150‑1 (MQ=255)
                                                                                                                                                gTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATCCACCAGCTGGCTGAAAATCACATTGTCATTTTCGTCAATAACCACAACGGCACGCGCTGCCAGACCtt       >  1:2463494/1‑150 (MQ=255)
                                                                                                                                                  gCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATCCACCAGCTGGCTGAAAATCACATTGTCATTTTCGTCAATAACCACAACGGCACGCGCTGCCAGACCTTTc     >  1:1142433/1‑150 (MQ=255)
                                                                                                                                                   cAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGACAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATCCACCAGCTGGCTGAAAATAACATTGTCATTTTCGTCAATAACCACAACGGCACGCGCTGCCAGACCTTTCa    >  1:278042/1‑150 (MQ=255)
                                                                                                                                                     acacCATCAGGCATATTAAATTATGCTATCAGAACAGCCAGAGCTGCTTCTTAATCCGGCTCGTTGGTGATTTCATCCAACAGCTGGCTGAAAATCACATTGTCGTTTTCGTCAATAACCACAACGGCACGCGCTGCAAGACCTTTCAGt  >  1:2283213/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TTAACCCAGCTTTATCGCAATTATCCCAGCACGCGCAAACTGGCAAAACGTCTCGGCGTTTCACATACCGCGATTGCCAATAAGTTGCGGGAATATGGTCTGAGTCAGAAGAAGAACGAAGAGTAAGCGCGAATATGCCTGATGGTGCAACACCATCAGGCATATTAAATTATGCTTTCAGTACAGCCAGAGCTGCTTCGTAATCCGGCTCGGTGGTGATTTCATCCACCAGCTGGCTGAAAATCACATTGTCATTTTCGTCAATAACCACAACGGCACGCGCTGCCAGACCTTTCAGT  >  NC_000913/1388136‑1388434

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: