Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,720,364 G→T 9.6% intergenic (+21/+26) slyB → / ← slyA outer membrane lipoprotein/global transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,720,3640GT 9.6% 534.4 / 8.3 178intergenic (+21/+26)slyB/slyAouter membrane lipoprotein/global transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (92/61);  new base T (2/23);  total (94/84)
Fisher's exact test for biased strand distribution p-value = 6.02e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 185 total aligned reads displayed.

CTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGT‑TTCTCCGCGCTAAATA‑AGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGTTAATAACAGCT  >  NC_000913/1720215‑1720513
                                                                                                                                                       |                                                                                                                                                     
cTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGT‑TTCTCCGCGCTAAATA‑AGTTTGCGTGTGGTCAgg                                                                                                                                                       >  2:165587/1‑150 (MQ=255)
 tGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGT‑TTCTCCGCGCTAAATA‑AGTTTGCGTGTGGTCAGGt                                                                                                                                                      >  2:3614590/1‑150 (MQ=255)
  ggAAATTCGTAAAGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGT‑TTCTCCGCGCTAAATA‑AGTTTGCGTGTGGTCAGGtt                                                                                                                                                     >  1:918369/1‑150 (MQ=255)
   gAAATTCGTAAAGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGT‑TTCTCCGCGCTAAATA‑AGTTTGCGTGTGGTCAGGTTa                                                                                                                                                    >  1:2905601/1‑150 (MQ=255)
     aaTTCGTAAAGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGT‑TTCTCCGCGCTAAATA‑AGTTTGCGTGTGGTCAGGTTACt                                                                                                                                                  <  1:3339053/150‑1 (MQ=255)
     aaTTCGTAAAGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGT‑TTCTCCGCGCTAAATA‑AGTTTGCGTGTGGTCAGGTTACt                                                                                                                                                  <  1:826284/150‑1 (MQ=255)
        tCGTAAAGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGT‑TTCTCCGCGCTAAATA‑AGTTTGCGTGTGGTCAGGTTACTGAc                                                                                                                                               <  1:316141/150‑1 (MQ=255)
        tCGTAAAGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGT‑TTCTCCGCGCTAAATA‑AGTTTGCGTGTGGTCAGGTTACTGAc                                                                                                                                               <  1:316140/150‑1 (MQ=255)
        tCGTAAAGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGT‑TTCTCCGCGCTAAATA‑AGTTTGCGTGTGGTCAGGTTACTGAc                                                                                                                                               <  1:3063084/150‑1 (MQ=255)
          gTAAAGACGTTGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTGTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGT‑TTCTCCGCGCTAAATA‑AGTTTGCGTGTGGTCTGGTTACTGTCca                                                                                                                                             <  2:1257728/150‑1 (MQ=255)
             aaGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGT‑TTCTCCGCGCTAAATA‑AGTTTGCGTGTGGTCAGGTTACTGACCacac                                                                                                                                          >  1:3819264/1‑150 (MQ=255)
             aaGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGT‑TTCTCCGCGCTAAATA‑AGTTTGCGTGTGGTCAGGTTACTGACCacac                                                                                                                                          <  2:500793/150‑1 (MQ=255)
             aaGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGT‑TTCTCCGCGCTAAATA‑AGTTTGCGTGTGGTCAGGTTACTGACCacac                                                                                                                                          <  2:500787/150‑1 (MQ=255)
               gACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGT‑TTCTCCGCGCTAAATA‑AGTTTGCGTGTGGTCAGGTTACTGACCACACGc                                                                                                                                        >  1:72645/1‑150 (MQ=255)
               gACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGT‑TTCTCCGCGCTAAATA‑AGTTTGCGTGTGGTCAGGTTACTGACCACACGc                                                                                                                                        >  1:2301034/1‑150 (MQ=255)
                 ctttgttAATACAATTTTGGTGTTACAGAAACATGGCAACACGGTTTTCTCTCGGGGCCAACGTGTCGTACTGGCCAGCATTGGCTTTCTGGTGACGTT‑TTCTCCGCGATAAATA‑TTTTTGGGTGTGGTCTGTTTACTCACCACACGccc                                                                                                                                      <  2:604988/144‑1 (MQ=255)
                             catcatGGTGTTACTGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCTGTCTGGTGACGTT‑TTCTCCGCGCTAAATA‑AGTTTGCGTTTGGTCTGGTTACTGACCACACGCCCCCTTCATTTCAc                                                                                                                          <  2:3092542/150‑1 (MQ=255)
                             catcatGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGT‑TTCTCCGCGCTAAATA‑AGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCAc                                                                                                                          >  1:2462809/1‑150 (MQ=255)
                             aatattggtgtTACAGAAACAAGGCAACACGCTTTTCTCTCGGGGCAACGGTGTGGTACTGGCCTGAAAGGGCTGCCTGGTGCCCTT‑TTCTCCGCGCTAAATA‑TTTTTGGGTTTGTTCTGTTTACTGACCACACGCCCCCTTCATTTCAc                                                                                                                          <  2:2789582/145‑1 (MQ=255)
                                     tggtACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGT‑TTCTCCGCGCTAAATA‑AGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGc                                                                                                                  >  2:689709/1‑150 (MQ=255)
                                     tggtACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGT‑TTCTCCGCGCTAAATA‑AGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGc                                                                                                                  >  1:595694/1‑150 (MQ=255)
                                      ggtACATAACCAAGGAAACACTCTTTTCACTCGGGGCAAAGGTGTGGTACTGGCCAGCAAGGGCTTTCTGGGGCCGGT‑TTCGCCGCGCTAAATA‑AGTTTGTGTGTGGTCTGTTTCGTGACCACACGCCCCCTTCATTTCACCCTTTGGcc                                                                                                                 <  2:1915585/150‑1 (MQ=255)
                                       gtACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGT‑TTCTCCGCGCTAAATA‑AGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCt                                                                                                                >  1:1830186/1‑150 (MQ=255)
                                        tACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGT‑TTCTCCGCGCTAAATA‑AGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTg                                                                                                               >  1:2463177/1‑150 (MQ=255)
                                         aCAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGT‑TTCTCCGCGCTAAATA‑AGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGt                                                                                                              >  1:3106427/1‑150 (MQ=255)
                                          cAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGTCCGT‑TTCTCCGCGCTAAATA‑AGTTTGCGTGTGGTCAGGTTACTGACGACTCGGCCGCTTCATTTCTCCCTTTGGCCTGTa                                                                                                             >  1:3077458/1‑150 (MQ=255)
                                          cAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGT‑TTCTCCGCGCTAAATA‑AGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTa                                                                                                             >  1:2892091/1‑150 (MQ=255)
                                          cAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGT‑TTCTCCGCGCTAAATA‑AGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTa                                                                                                             >  1:4004294/1‑150 (MQ=255)
                                          cAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGT‑TTCTCCGCGCTAAATA‑AGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTa                                                                                                             >  2:1770989/1‑150 (MQ=255)
                                           aGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGT‑TTCTCCGCGCTAAATA‑AGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTaa                                                                                                            >  1:2161194/1‑150 (MQ=255)
                                             aaaCAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGT‑TTCTCCGCGCTAAATA‑AGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACt                                                                                                          >  1:911566/1‑150 (MQ=255)
                                                cAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGT‑TTCTCCGCGCTAAATA‑AGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCaa                                                                                                       >  1:1277034/1‑150 (MQ=255)
                                                 aaGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGAGACCGT‑TTCTCCGCGCTAAATA‑AGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCAAATCACCCTTACGCCAGTAACTCAAt                                                                                                      >  2:1257543/1‑150 (MQ=255)
                                                   ggCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGT‑TTCTCCGCGCTAAATA‑AGTTTTCGTGTGGTCAGGTTAATGACCACAAGACACCTTCATTTCACACTTTGGCCTGTAACTCAATGa                                                                                                    >  1:4022055/1‑150 (MQ=255)
                                                    gCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGT‑TTCTCCGCGCTAAATA‑AGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGat                                                                                                   >  1:743649/1‑150 (MQ=255)
                                                     cAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGT‑TTCTCCGCGCTAAATA‑AGTTTGCGTGTGGTCAGGTTACTGa                                                                                                                                                >  2:3988739/1‑104 (MQ=255)
                                                        caggggTTTCTCTCGGGGCCAACGTGTCTTAGTGGCAAGCTTTGGCTGTCTGGTGACCGT‑TTCTCCGCGCTAATTA‑TGTTTGCTTGTTTTCTTGTTCCGGACCCCACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGAtatta                                                                                               <  2:802748/145‑1 (MQ=255)
                                                        cacgggTTTCTCTCGGGGCCACGGTGTCGTCGTGGCCAGCACTGGCAGTCGGGTG‑CCGTGTTCTCGGCGATAAATA‑ATTTTGGGTTTGGGCTGTTTACTCACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGAtatta                                                                                               <  2:1514191/145‑1 (MQ=255)
                                                         acTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGT‑TTCTCCGCGCTAAATA‑AGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTCACTCAATGAtattat                                                                                              >  2:3323062/1‑150 (MQ=255)
                                                           tCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGT‑TTCTCCGCGCTAAATA‑AGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGc                                                                                            >  1:2220153/1‑150 (MQ=255)
                                                           tCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGT‑TTCTCCGCGCTAAATA‑AGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCAGTTCACCCTTTGGCCTGTAACTCAATGATATTATGc                                                                                            >  2:2717242/1‑150 (MQ=255)
                                                               ttCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGT‑TTCTCCGCGCTAAATA‑AGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCaa                                                                                        >  1:341816/1‑150 (MQ=255)
                                                               ttCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGT‑TTCTCCGCGCTAAATA‑AGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCaa                                                                                        >  1:342098/1‑150 (MQ=255)
                                                                    tcCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGT‑TTCTCCGCGCTAAATA‑AGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGtttt                                                                                   >  2:810073/1‑150 (MQ=255)
                                                                    tcCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGT‑TTCTCCGCGCTAAATA‑AGTTTGCGAGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGattt                                                                                   >  1:2488554/1‑150 (MQ=255)
                                                                     cggggCCAAGGTGTCGTAGTGGCCAGCATTGGATGTCTGGTGACCGT‑TTCTCCGCGATAAATA‑CGTTTGGGTGTGGTCTGTTTACTCACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTg                                                                                  <  2:87552/148‑1 (MQ=255)
                                                                      cGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGT‑TTCTCCGCGCTAAATA‑AGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGc                                                                                 >  1:3979164/1‑150 (MQ=255)
                                                                      cGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGT‑TTCTCCGCGCTAAATA‑AGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGc                                                                                 >  2:4099857/1‑150 (MQ=255)
                                                                      cGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGT‑TTCTCCGCGCTAAATA‑AGTTTGCGTGTGGTCAGGTTACTGACAACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGATCAAGTTTTGc                                                                                 >  2:4100109/1‑150 (MQ=255)
                                                                       gggCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGT‑TTCTCCGCGCTAAATA‑AGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCg                                                                                >  2:484382/1‑150 (MQ=255)
                                                                         gCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACAGT‑TTCTCCGCGCTAAATA‑AGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATATCACCCTTTGGCCTGTAAATCAAAGATATTATGCTCAAGTTTTGCGAt                                                                              >  1:1307717/1‑150 (MQ=255)
                                                                                tgtCGTACTGGCCAGCAATGGCAGTCAGGTGACCGT‑TTCTCCGCGCTAAATA‑AGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTa                                                                       >  1:3038706/1‑150 (MQ=255)
                                                                                  tCGTACTGGCCAGCAATGGCAGTCAGGTGACCGT‑TTCTCCGCGCTAAATA‑AGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAAt                                                                     >  2:2366993/1‑150 (MQ=255)
                                                                                   cttcggggCCCGCACGGGCTGTCCGTTGACGTT‑TTCGCCGCGATAAATT‑TTTTGGGGTGTGTTCTGTTTACTCACCCCACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATc                                                                    <  2:1086675/144‑1 (MQ=255)
                                                                                   cggacgggCCAGCAATGGCAGTCCGGTGACCGT‑TTCTCCGCGCTAAATC‑AGTTTGCGTGTGGGCTGGCTACTGACCACACGCCCCCTTCATTTCCCCCTTTGGCCTGTAACTCACTGATATAATGCTCAAGTTTTGCGATGAGCGTAATc                                                                    <  2:1735052/144‑1 (MQ=255)
                                                                                     ttcTGGCCAGCATTGGCTGTCAGGTGACCGT‑TTCTCCGGGCTATATA‑AGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAg                                                                  <  2:2367574/148‑1 (MQ=255)
                                                                                        tGGCCACCATTGGCTGTCCGGTGACCGT‑TTCTCCGCTATAAATA‑TTTTGGGGTGTGTCCAGGTTACTCACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTg                                                               <  1:3189338/150‑1 (MQ=255)
                                                                                         ggCCAGCATGGGCAGTCTGGTGACCGT‑TTCGCCGCGATAAATA‑TGTTTGGGTGTGGTCTGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGc                                                              <  2:2161194/150‑1 (MQ=255)
                                                                                           ccAGAATTGGCTGTCTGGGGACCTT‑TTCTCCGCGCTAAATA‑AGTTTGCTTGTGTTCTGGTAACTCACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTc                                                            <  2:2905601/150‑1 (MQ=255)
                                                                                           caagaaaTGGCTGTCAGGTGACCGT‑TTCTCCGCGCTAAATA‑AGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGGCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTc                                                            <  2:1305129/145‑1 (MQ=255)
                                                                                            cAGCAATGGCAGTCAGGTGACCGT‑TTCTCCGCGCTAAATA‑AGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTcc                                                           >  2:3444209/1‑150 (MQ=255)
                                                                                                     ctgTCGGGTCACCTT‑TTCCCCGCTCTAAATT‑CTTTGGGGTGTGGTATGGTTACTCCCCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCctct                                                  <  1:1203242/148‑1 (MQ=255)
                                                                                                      tgTCTGGTGCCCGT‑TTCCCCGCTATAAATA‑TGTTTGCGTGTGTTCTGTTTCCTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTg                                                 <  1:1335071/149‑1 (MQ=255)
                                                                                                       gTCTGGTCACGGT‑TTCTCCGC‑CAAAATATTGTTGGGGTGTGGTCTGGTTACTCACCCCACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGc                                                <  1:3834933/150‑1 (MQ=255)
                                                                                                        tctggTGACCTT‑TTCCCCGCGCTAAATA‑TGTTGGCGTGTGGTCTGTTTACTCACCCCACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCg                                               <  1:2800591/147‑1 (MQ=255)
                                                                                                        tCAGGTGACCGT‑TTCTCCGCGCTAAATA‑AGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCg                                               <  2:341863/150‑1 (MQ=255)
                                                                                                         cAGGGGACCGT‑TTCTCCGCGATAAATA‑TGTTTGCGTGTGGTCTGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCgg                                              <  2:2874541/150‑1 (MQ=255)
                                                                                                            gTGACCGT‑TTCTCCGCGCTAAATA‑AGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGGTCCTCTGCGgagc                                           >  2:2601879/1‑149 (MQ=255)
                                                                                                               aCCGT‑TTCTCCGCGCTAAATA‑AGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGc                                        >  1:257496/1‑150 (MQ=255)
                                                                                                                cggT‑TTATCCGGGCTAAATA‑AGTTTGGGTGGTGTCATTTTACTTACCACTCGCCCCCTTCATTTCACCCTTTGGCATGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGcc                                       <  2:3671056/148‑1 (MQ=255)
                                                                                                                 cGT‑TTCTCCGCGCTAAATA‑AGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGCTGCCa                                      >  2:3949924/1‑150 (MQ=255)
                                                                                                                  ctttcccccGCGAAAAATC‑AGTTTGGGTGGGGCCTGGTAACGGACCCCACGCCCCCTTCATGTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCa                                      <  1:1613111/143‑1 (MQ=255)
                                                                                                                   t‑ttCTCCGCGCTAAATA‑AGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATg                                    >  1:2534630/1‑150 (MQ=255)
                                                                                                                     ttCCCCGCGCTAAATA‑TGTTGGCGTGTGGTCTGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGt                                   <  1:4113621/150‑1 (MQ=255)
                                                                                                                      tctcCGCGCTAAATA‑AGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTa                                  <  2:2201483/150‑1 (MQ=255)
                                                                                                                      tctcCGCGATAAATT‑TGTTTGTGTGTGGTCTGGTTACTCACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTa                                  <  1:165587/150‑1 (MQ=255)
                                                                                                                        tcCGCGCTAAATA‑AGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAat                                >  2:3819579/1‑150 (MQ=255)
                                                                                                                          cgcgCTAATTA‑TTTTTGCGTGTGGTCAGTTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAatat                              <  2:2462809/150‑1 (MQ=255)
                                                                                                                            cgcTAAATA‑AGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCCTGTAATAttt                            >  2:3797652/1‑150 (MQ=255)
                                                                                                                            cgcTAAATA‑AGTTTGCGTGTGGTCAGGTTAATGACCACACGCCCCCTTCATTTCACCCATTGGCCTGTAAATCAATGATATTATGCTCAAGTTTTGCGATGTGCGGAATAAGTTGCTCCAGTTCCTATGCGGGGATGCCCTGGACTAttt                            >  1:3910513/1‑150 (MQ=255)
                                                                                                                              atAAATA‑AGTTGGCGTGTGGTCTGGTTAATGACAACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTcc                          <  2:595694/149‑1 (MQ=255)
                                                                                                                                  tttttgTTTGGGTGGGTTCTGTTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCcgcg                       <  2:1431218/145‑1 (MQ=255)
                                                                                                                                  aTA‑AGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCcgcgc                      >  2:3495063/1‑150 (MQ=255)
                                                                                                                                    ttaGTTGGGGTGTGGTCCGGTTCCTCACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCcgcgcg                     <  2:3140885/148‑1 (MQ=255)
                                                                                                                                    tatgTTTGCGTGTGGTCTGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCcgcgcg                     <  1:3442005/147‑1 (MQ=255)
                                                                                                                                     atttttGCGTGGGTTCTGTTTACGGACCCCACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCgg                    <  1:67966/147‑1 (MQ=255)
                                                                                                                                         ttGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGttt                <  2:2646373/150‑1 (MQ=255)
                                                                                                                                         tggggtgtGGTCTGTTTACTCACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGttt                <  1:1929931/146‑1 (MQ=255)
                                                                                                                                           gCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTg              <  2:3414468/150‑1 (MQ=255)
                                                                                                                                            cGTGTGGTCAGTTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGt             <  2:233385/150‑1 (MQ=255)
                                                                                                                                              tgtgGTCAGTAACCTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGTta           >  2:1201938/1‑150 (MQ=255)
                                                                                                                                               gtgGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGTtaa          >  1:3740326/1‑150 (MQ=255)
                                                                                                                                                ttgacAGGATACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGTtaat         <  2:944216/146‑1 (MQ=255)
                                                                                                                                                tgGTCTGTTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGTtaat         <  1:3365626/150‑1 (MQ=255)
                                                                                                                                                 ggTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGTtaata        >  1:2677331/1‑150 (MQ=255)
                                                                                                                                                    cAGTTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGTTAATAACa     <  1:1018121/150‑1 (MQ=255)
                                                                                                                                                    cAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGTTAATAACa     >  2:2020891/1‑150 (MQ=255)
                                                                                                                                                      ggTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGTTAATAACAGc   >  2:1809035/1‑150 (MQ=255)
                                                                                                                                                      agTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGTTAATAACAGc   >  1:2013401/2‑150 (MQ=255)
                                                                                                                                                       gTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGTTAATAACAGCt  >  1:362327/1‑150 (MQ=255)
                                                                                                                                                       |                                                                                                                                                     
CTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGGCAGTCAGGTGACCGT‑TTCTCCGCGCTAAATA‑AGTTTGCGTGTGGTCAGGTTACTGACCACACGCCCCCTTCATTTCACCCTTTGGCCTGTAACTCAATGATATTATGCTCAAGTTTTGCGATGAGCGTAATCAGTTGCTCCAGTTCCTCTGCGGAGATGCCATGTAATATTTCCGCGCGGGTTTTGTTAATAACAGCT  >  NC_000913/1720215‑1720513

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑