| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 164,620 | C→T | 5.4% | intergenic (+86/‑110) | hrpB → / → mrcB | putative ATP‑dependent helicase/fused glycosyl transferase and transpeptidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 164,620 | 0 | C | T | 5.4% | 481.5 / 11.1 | 148 | intergenic (+86/‑110) | hrpB/mrcB | putative ATP‑dependent helicase/fused glycosyl transferase and transpeptidase |
| Reads supporting (aligned to +/- strand): ref base C (52/87); new base T (3/5); total (56/92) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 149 total aligned reads displayed.
ATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTG‑TCT‑‑TGT‑‑‑‑A‑ACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGGGA > NC_000913/164473‑164769 | aTGTCGGGCCGGACGACCCGGCAAATCCTGCACCGACGCGACGGACGAAAAAGTTTTCGTACGGTGGGTTTTCCCCCCCGCCGGGTGGGTGAGGGCGGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCaa < 2:188782/150‑1 (MQ=255) gtGGCCGGACGACCGGGCAAATCCCGCACCGACGCGCCGGACGAAAATGTATGCGTATGGTTGGTTTGCGCCCTCTCCCTGTGGGTGCGGGCGGGGGTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGga < 1:3962434/149‑1 (MQ=255) cgggCCGGACGCCCCGGAAAATACTGCACCGACGCGACGGACTAAAATGTATTCGTATGGTTGGTTTGCCCCCTCTCCGGGGGGGAGTGGGCGGGGGTGTGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGga < 1:3962516/148‑1 (MQ=255) cTGGCCGGACGACCCGGCAAATACTGCACCGAGGCGACGGACGAAAACTTTTTGGTTCGGTTGGTTTTCGCCCTCTCCCTGTGGTAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGga < 2:2802809/150‑1 (MQ=37) gCCGGACGCCCCTCAAAAACCTCCACCGCCGCGCCGGCCTAAAATTTTTTCGTAGGTTTGTTTTCCCCCCACGCGGTGGGGGCGTGGGCGGGGGTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGagag < 1:585107/150‑1 (MQ=255) gggACGACCCGGCAAATCCTGCACCGACGCGCCGGACGAAAATTTTTTCGTATGGTTGGTTTCCTCCCTCTCCCTGGGGGTGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAggg < 1:3054329/149‑1 (MQ=38) cgcacgacCGGGCCAATACTGCACCGACGGGAGGGACTTAAAAGTATTCTTACTGTGGGTTTTCTCCAACTCCGTGTGGGAGTGGGCCGGGGGGAGGGCATCAGCGCGCCCGGTCTCCCTCACCCTAACCCTCTCCCTTGAGGGAGAggg < 2:1686575/147‑1 (MQ=255) cGGCCGACCCGGCAAATACTGCCCCGACGCGAGGGACTAAAAAGTTTTCTTACGGTTGGTTTTCGCCCCCCCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAggg < 2:3254692/150‑1 (MQ=255) cGGACGACCCGGCAAATCCTGCACCGCCGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAggg < 2:592033/150‑1 (MQ=255) cGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCCGCGCGCACGTGGCCCCACACCCCGACCCCCACCCACCAGGGGGAggg > 1:1127625/1‑150 (MQ=25) acCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTGACCCCCACCCACAACCACACCCCCAAAGGAGAGGGGGcccc > 2:2055698/1‑148 (MQ=25) acCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGGAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGGGCACCCGCACCCACAACCGCACCCGCCAGGGGGGGGGGGCCgc > 2:4089293/1‑149 (MQ=25) ggCAATCACGGCACCGCCGGGCCGGACTAAAATGTATTCGTATGGTTGGTTTCCGCCCTCTCCGTGGGGGTGCGGGCGGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGa < 1:2749863/150‑1 (MQ=255) ggCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCGGCGCGCACGTTCACCCGCACCCGAAACCAATACCTCAAGGGGGTGGGGAGCggtggg > 2:3312620/1‑145 (MQ=25) ggCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTGACCCGCACCCGAACCCACTCCCACCAGGGGGCGGGGGCCgcggga > 2:3312590/1‑145 (MQ=25) aaaaACTCCACCGACGCGACGGACTAAAATGTATTCGTACGGTTGGTTTACCCCCTCTCCGTGTGGGTGTGGGCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGca < 1:3000004/150‑1 (MQ=255) aaaTACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGGTGACCCACACCCGAACCCTCACCCACAACGGGGAGGGGGCCCAtacagcc > 2:4050333/1‑144 (MQ=25) aaTACTGCACCGACGCGACGGACGAAAAAGTATTCGTATGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGcac < 2:4119482/150‑1 (MQ=255) tACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCAACCACACCCCAACCCAAACCCACAAGGGAGGGGGGACCGCGCGAGCCCaa > 2:1326277/1‑150 (MQ=25) cTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGCCCCGCACCCCAACCCACACCCACAACGGCGGGGGGCCCCATAGCGCACaaaa > 1:3012772/1‑149 (MQ=21) tGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCCGCGCGCACGGGCGCCCCCCCCCAACCCCCCAACCCCAACGGGGAGGGGGCCCCGCGAGCACcaaat > 2:96788/1‑148 (MQ=16) ccgccgCGCCGGACTAAAACGTATTCGTACGGTTGGTTTTCTCCCTCGCCGTGTGGTTGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTga < 2:3281316/150‑1 (MQ=255) ccGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTga < 2:3391177/150‑1 (MQ=255) acggactAAAAAGTTTTCGTATGGTTGGTTTTCTCCCTCTCCCTGTGGGTGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtc < 2:285867/150‑1 (MQ=255) aaaaaGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGCCCCACACCCCAAACCGCACCCGCAAGGGCGCGGGGGCCCAGCGAGCCCCAATTTTGTGGGATGtcttgttct > 2:2025801/1‑150 (MQ=16) attttttCGTATGTTTGTTTTTCCCCCTCGCCCGGTGGTTGTGGGCGGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTG‑Tc < 2:811252/145‑1 (MQ=255) aaGTATTCGTACGGTTGTTTTTCTCCCTCTCCCTGTGGGTGAGGGCCGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTG‑Tc < 2:953701/150‑1 (MQ=255) gtttcggttggttTACCCCCTCCCCGTGTGGGTGTGGGCGGGGGTGTGGGCCTCAGCGCGCACGGTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTG‑TCT‑‑TGT‑‑‑‑A‑Ac < 1:2021792/145‑1 (MQ=255) gTAAGGTTGTTTTTCTCCCTCTCCCTGTGGGAGAGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTG‑TCT‑‑TGT‑‑‑‑A‑ACa < 2:3012568/150‑1 (MQ=255) tAAGGTTGGTTTTCTCCCTCTCCCTGTGGGTGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTG‑TCT‑‑TGT‑‑‑‑A‑ACag < 1:2385231/150‑1 (MQ=255) atggttggttTGCTCCCTTTCCGTGTGGGTGTGGGCCGGGGTGTGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTG‑TCT‑‑TGT‑‑‑‑A‑ACaga < 1:864319/148‑1 (MQ=255) aaGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTG‑TCT‑‑TGT‑‑‑‑A‑ACaga < 2:1366492/150‑1 (MQ=255) aaGGTTGGTTTTCTCCCTCCCCCTGGGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTG‑TCT‑‑TGT‑‑‑‑A‑ACaga < 1:2976384/150‑1 (MQ=255) aGGGTGGTTTTCTCCCTCTCCCTGGGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTG‑TCT‑‑TGT‑‑‑‑A‑ACagaa < 2:2409315/150‑1 (MQ=255) tttgttTACTCCCCCTCGATGGGGGAGAGGGCGGGGTTGATGGCTTCAGCGCGCACGTTCACCCTCACCCTATCCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCTCAAATTTTGAGAGATATCTTATTCTG‑TCT‑‑TGT‑‑‑‑A‑ACAgaagaa < 1:1352225/147‑1 (MQ=255) tggttTTCTCCCGCGCCCTGTGGGAGAGGGCGGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTG‑TCT‑‑TGT‑‑‑‑A‑ACAGAAGAAc < 1:4119438/150‑1 (MQ=255) ggttTTCTCCCGCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCGAACCCTCTCCCTCAAGGGAGAGGGGACCGAACGAGCACAAATTTTGAGAGATATCTTCTTCTG‑TCT‑‑AGT‑‑‑‑A‑ACAGAAGAACa < 2:297121/150‑1 (MQ=255) tttCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGGTCACCCGCCCCCGAACCCACGCCCACAATGGGGAGGGGCCCGATCCAGCCCAAATTTTGAGAGATATCTTGTTCTG‑TCT‑‑TGT‑‑‑‑A‑ACAGAAGAACAGaa > 2:2059319/1‑150 (MQ=25) cctctccctGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGGTCACCCCACCCCAAACCCAAACCCCCACCGGCGAGGGGACCCGTCGGGCCCAAAATTTGAGAGATATATTGTTCTG‑TAG‑‑AGT‑‑‑‑A‑ACAGAAGGACCGAAAAGCgg > 2:307917/1‑150 (MQ=12) cctctccctGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCAAAACCTCACCCTCAAGGGGGTGGGGGCCGATCGAGCACAAATTTTGAGGGATATATTCTTCTG‑TCT‑‑TGT‑‑‑‑G‑AAAGAAGTAAAGAAAATCgg > 2:3094714/1‑150 (MQ=37) ccctGGGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTG‑TCT‑‑TGT‑‑‑‑A‑ACAGAAGAACAGAAAATCGGGCttt < 1:2620588/150‑1 (MQ=255) cctGGGGGAGTGGGCCGGGGTGAGGGCCTCAGCGCGCCCGTGCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTG‑TCT‑‑TGT‑‑‑‑A‑ACAGAAGAACAGAAAATCGGGCtttt < 1:1562785/150‑1 (MQ=255) gtgGGTGTGGGCGGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTG‑TCT‑‑TGT‑‑‑‑A‑ACAGAAGAACAGAAAATCGGGCTTTTgcg < 1:1557801/150‑1 (MQ=255) tgGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGCACCCACACCCAAAACCACAACCACCAGGGGGAGGGGGCCCATCGAGCCCAAAATTTGAGAGATAAAATATTATG‑TAT‑‑TGT‑‑‑‑A‑ACAGAAAAACATCAAAAAGGGCGTTTtcgc > 2:1376930/1‑150 (MQ=255) ggggtgcgggCGGGGGTGCGGCCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTG‑TCT‑‑TGT‑‑‑‑A‑ACAGAAGAACAGAAAATCGGGCTTTTgcgc < 1:1437939/143‑1 (MQ=255) ggggggtgggCGGGGGGGGGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTG‑TCT‑‑TGT‑‑‑‑A‑ACAGAAGAACAGAAAATCGGGCTTTTgcgc < 2:377491/143‑1 (MQ=255) ggggAGTGGGCCGGGGTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTG‑TCT‑‑TGT‑‑‑‑A‑ACAGAAGAACAGAAAATCGGGCTTTTgcgc < 1:3867645/149‑1 (MQ=255) ggggAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGGTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTG‑TCT‑‑TGT‑‑‑‑A‑ACAGAAGAACAGAAAATCGGGCTTTTgcgc < 1:3884677/149‑1 (MQ=255) cGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTG‑TCT‑‑TGT‑‑‑‑A‑ACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGc < 1:4108338/150‑1 (MQ=255) cGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTG‑TCT‑‑TGT‑‑‑‑A‑ACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGc < 1:434050/150‑1 (MQ=255) ggggTGAAGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTG‑TCT‑‑TGT‑‑‑‑A‑ACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCg < 2:610582/150‑1 (MQ=255) ggTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTG‑TCT‑‑TGT‑‑‑‑A‑ACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGga < 1:2711821/150‑1 (MQ=255) ggccATCAGCGCCCACGTTCACCCTCACCCTACCCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAAATTCGAGAGATATCTTCTTCTG‑TCT‑‑TGT‑‑‑‑A‑ACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGaaaa < 1:458293/147‑1 (MQ=255) tCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTG‑TCT‑‑TGT‑‑‑‑A‑ACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCAt < 2:3969176/150‑1 (MQ=255) cAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTG‑TCT‑‑TGT‑‑‑‑A‑ACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATg < 1:406393/150‑1 (MQ=255) cgcgcACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTG‑TCT‑‑TGT‑‑‑‑A‑ACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGcc < 2:2929586/150‑1 (MQ=255) cGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTG‑TCT‑‑TGT‑‑‑‑A‑ACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAAt < 2:484181/150‑1 (MQ=255) ttCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTG‑TCT‑‑TGT‑‑‑‑A‑ACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGa < 2:4072574/150‑1 (MQ=255) ttCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTG‑TCT‑‑TGT‑‑‑‑A‑ACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGa < 2:2805426/150‑1 (MQ=255) ttCACCCTCAACCTAACACAAAACCAAAAGGGAGAGGGGCCCGATCGAGCACAAATTTTGAGAGATAAATTCTTCTG‑TAT‑‑TGT‑‑‑‑A‑ACAGAAGAACAGAAAATAGGGCTTTTTCGCCGGAATATTTCGGAGAAAAAGCAAGGCCCGGAATGa > 2:2806713/1‑150 (MQ=255) caccctcaccctAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTG‑TCT‑‑TGT‑‑‑‑A‑ACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGAcc < 2:4051553/150‑1 (MQ=255) ctcaccctAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTG‑TCT‑‑TGT‑‑‑‑A‑ACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGACTGACCGCGa > 1:3410238/1‑150 (MQ=255) ctcaccctAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTG‑TCT‑‑TGT‑‑‑‑A‑ACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGa > 1:1357841/1‑150 (MQ=255) ctcaccctAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTG‑TCT‑‑TGT‑‑‑‑A‑ACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGa > 1:2733391/1‑150 (MQ=255) ctcacccgACCCCTCTCCCTCACGGGAGAGGGGCCCGATCGAGCACAAAGGTTGAGAGATATCTTCTTCTG‑TCT‑‑TGT‑‑‑‑A‑ACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATGGCGGAGAAAAAGCATGGCCGGGAATGACCGCGa < 1:2005657/150‑1 (MQ=255) tcaccctAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTG‑TCT‑‑TGT‑‑‑‑A‑ACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAg < 1:1655749/150‑1 (MQ=255) tcaccctAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTG‑TCT‑‑TGT‑‑‑‑A‑ACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAg < 2:338824/150‑1 (MQ=255) caccctACCCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTG‑TCT‑‑TGT‑‑‑‑A‑ACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGc < 2:822990/150‑1 (MQ=255) cctAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTG‑TCT‑‑TGT‑‑‑‑A‑ACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAACAGCATGGCCGGGAATGACCGCGAGCCaa > 2:2263268/1‑150 (MQ=255) cctAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTG‑TCT‑‑TGT‑‑‑‑A‑ACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCaa > 2:2263306/1‑150 (MQ=255) cctAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTG‑TCT‑‑TGT‑‑‑‑A‑ACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCaa > 2:481164/1‑150 (MQ=255) tAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGATCACAAATTTTGAGAGATATCTTCTTCTG‑TCT‑‑TGT‑‑‑‑A‑ACAGAAGAACAGAAAATCGGGCTTTTGCGCATGATTATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAAtt < 2:2014534/150‑1 (MQ=255) tAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTG‑TCT‑‑TGT‑‑‑‑A‑ACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGTATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAAtt < 1:3440302/150‑1 (MQ=255) tAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTG‑TCT‑‑TGT‑‑‑‑A‑ACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAAtt < 2:773930/150‑1 (MQ=255) tAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTG‑TCT‑‑TGT‑‑‑‑A‑ACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAAtt < 1:1260942/150‑1 (MQ=255) tAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTG‑TCT‑‑TGT‑‑‑‑A‑ACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAAtt < 2:48429/150‑1 (MQ=255) gtccccTCTCCCTTGAGGGAGAGGGTACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTG‑TCT‑‑TGT‑‑‑‑A‑ACagaa < 1:11191/74‑1 (MQ=37) gtccccTCTCCCTTGAGGGAGAGGGTACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTG‑TCT‑‑TGT‑‑‑‑A‑ACagaa > 2:11191/4‑77 (MQ=37) aaCCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTTTTCTGTTCTTCTGTTACAAGACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGa < 1:2036110/150‑1 (MQ=255) aaCCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTG‑TCT‑‑TGT‑‑‑‑A‑ACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTg < 1:1616628/150‑1 (MQ=255) cccTCTCCCTTGAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTG‑TCT‑‑TGT‑‑‑‑A‑ACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGa > 1:853933/1‑150 (MQ=255) cccTCTCCCTCACGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGAGATCTTCTTCTG‑TCT‑‑TGT‑‑‑‑A‑ACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGa < 1:4036839/150‑1 (MQ=255) cccTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTG‑TCT‑‑TGT‑‑‑‑A‑ACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGa < 2:1031077/150‑1 (MQ=255) cccTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTG‑TCT‑‑TGT‑‑‑‑A‑ACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGa > 2:1948620/1‑150 (MQ=255) ccTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTG‑TCT‑‑TGT‑‑‑‑A‑ACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGAc > 1:3230224/1‑150 (MQ=255) ctctCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTG‑TCT‑‑TGT‑‑‑‑A‑ACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGACTGACCGCGAGCCAATTGGACg > 1:2246295/1‑150 (MQ=255) ctctCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTG‑TCT‑‑TGT‑‑‑‑A‑ACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACg > 1:94228/1‑150 (MQ=255) ctctCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTG‑TCT‑‑TGT‑‑‑‑A‑ACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACg > 1:1784146/1‑150 (MQ=255) ctctCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTG‑TCT‑‑TGT‑‑‑‑A‑ACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACg > 1:2172320/1‑150 (MQ=255) ctcCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTG‑TCT‑‑TGT‑‑‑‑A‑ACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCa > 1:2029186/1‑150 (MQ=255) tcCCTCTAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTG‑TCT‑‑TGT‑‑‑‑A‑ACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCaa < 2:2429102/150‑1 (MQ=255) ccTCACGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTG‑TCT‑‑TGT‑‑‑‑A‑ACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAg < 2:3627882/150‑1 (MQ=255) ccTCAAGGGAGAGGGGACCGATCGAGCACAACTTTTGAGAGATATCTTCTTCTG‑TCT‑‑TGT‑‑‑‑A‑ACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAg < 2:2125696/150‑1 (MQ=255) ccTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTG‑TCT‑‑TGT‑‑‑‑A‑ACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAg > 2:2999534/1‑150 (MQ=255) cTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTG‑TCT‑‑TGT‑‑‑‑A‑ACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAgg > 2:365189/1‑150 (MQ=255) tCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTG‑TCT‑‑TGT‑‑‑‑A‑ACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAggg > 2:755779/1‑150 (MQ=255) tCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTG‑TCT‑‑TGT‑‑‑‑A‑ACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAggg > 2:2216185/1‑150 (MQ=255) tCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTG‑TCT‑‑TGT‑‑‑‑A‑ACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAggg > 1:1456008/1‑150 (MQ=255) tCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTG‑TCT‑‑TGT‑‑‑‑A‑ACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAggg > 2:1563089/1‑150 (MQ=255) cAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCGG‑TCT‑‑TGT‑‑‑‑A‑ACAGAAGAACAGAAACTCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGGGa < 1:1753502/150‑1 (MQ=255) | ATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTG‑TCT‑‑TGT‑‑‑‑A‑ACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCCAATTGGACGCAAAGGGA > NC_000913/164473‑164769 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |