Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,470,431 G→T 13.0% R434I (AGA→ATA)  gtrS → serotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,470,4310GT13.0% 445.3 / 2.8 164R434I (AGA→ATA) gtrSserotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage
Reads supporting (aligned to +/- strand):  ref base G (58/78);  new base T (17/3);  total (83/81)
Fisher's exact test for biased strand distribution p-value = 5.07e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 168 total aligned reads displayed.

TCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGA  >  NC_000913/2470286‑2470580
                                                                                                                                                 |                                                                                                                                                     
tctcTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAAt                                                                                                                                                   <  1:1961471/150‑1 (MQ=255)
 ctctTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAATAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATc                                                                                                                                                  <  1:3775040/150‑1 (MQ=255)
  tctTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCa                                                                                                                                                 <  2:2115702/150‑1 (MQ=255)
  tctTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGTAATCa                                                                                                                                                 >  2:242942/1‑150 (MQ=255)
   ctTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAATAAAACaa                                                                                                                                                >  1:1421083/1‑150 (MQ=255)
    tttttGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATTAAATAATTCAAAGTAATGAAg                                                                                                                                               >  2:1377987/1‑150 (MQ=255)
    tttttGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATAAaaataaatacag                                                                                                                                               >  2:2447730/1‑146 (MQ=255)
          gAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATa                                                                                                                                         <  1:713551/150‑1 (MQ=255)
          gAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATa                                                                                                                                         >  2:3660777/1‑150 (MQ=255)
            atatCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGc                                                                                                                                       <  1:190795/150‑1 (MQ=255)
              atCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGa                                                                                                                                     <  1:1410898/150‑1 (MQ=255)
              atCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGa                                                                                                                                     <  1:3010814/150‑1 (MQ=255)
              atCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGa                                                                                                                                     <  1:893151/150‑1 (MQ=255)
               tctcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATAAAGAAAATAGCGAt                                                                                                                                    >  2:2734646/1‑150 (MQ=255)
                 tcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAATAAATCAAGACAAAAGCGATGt                                                                                                                                  >  2:251075/1‑150 (MQ=255)
                 tcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAATAAATAAAAACAAAAGAGAttt                                                                                                                                  >  1:2409282/1‑148 (MQ=255)
                  cAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAATAAATAAATAAAATAGCGAtttt                                                                                                                                 >  2:3626631/1‑147 (MQ=255)
                    aTTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAATAAATACAAAATAAATGTaaa                                                                                                                               >  1:1104039/1‑150 (MQ=255)
                     ttttATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAg                                                                                                                              <  1:1260343/150‑1 (MQ=255)
                       ttATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCa                                                                                                                            <  2:2693150/150‑1 (MQ=255)
                       ttATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCa                                                                                                                            <  2:587237/150‑1 (MQ=255)
                            tgtCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTTATTTATTAAATAATTCAAATAAATCAAGAAAATATCGCTTTAAATCACGAAg                                                                                                                       >  1:1816716/1‑150 (MQ=255)
                            tgtCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAATAAATCAAGACAATAGCGATGTAAAGCACTAAg                                                                                                                       >  2:1610611/1‑150 (MQ=255)
                             gtCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAATAAAAAAAGAAAAAAGCGATTTAAATCACTAAGt                                                                                                                      >  1:632500/1‑150 (MQ=255)
                              tCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAAAAAAAACAAAATCGATTTAAAGAAAAAAGtt                                                                                                                     >  2:2303078/1‑150 (MQ=255)
                              tCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAAAAAAAAAGAAAAAAGCGATGTAAAGCACTAAGtt                                                                                                                     >  1:2068296/1‑150 (MQ=255)
                              tCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAAATTATAAAAAAAATAAAAAAAAAAAAGAAAAAATCGATGTAAATCACAAAAtt                                                                                                                     >  2:3841174/1‑150 (MQ=255)
                                aTTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAAATCAAATAAATAAAGAAAAAAGCGATGTAAATCACTAACTTTa                                                                                                                   >  1:4012168/1‑150 (MQ=255)
                                  ttttCGGGGAAGGATATAGAGATTTAAGCAAGGATTTATTTGGAATGTTTTTTTCGTTCGACCTTTGCTTATACATAACTGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTaaa                                                                                                                 <  1:3129906/150‑1 (MQ=255)
                                     tCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAATCAAGAAAATAGCGATGTAAAGCACTAAGTTTAAAttt                                                                                                              >  1:338763/1‑149 (MQ=255)
                                       ggggAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTgcg                                                                                                            <  2:868605/150‑1 (MQ=255)
                                        gggAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTgcgc                                                                                                           <  1:3895484/150‑1 (MQ=255)
                                        gggAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTgcgc                                                                                                           <  1:937159/150‑1 (MQ=255)
                                          gAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTgcgcgc                                                                                                         <  1:14118/150‑1 (MQ=255)
                                          gAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTgcgcgc                                                                                                         <  2:3279957/150‑1 (MQ=255)
                                           aaGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGcc                                                                                                        <  1:3223623/150‑1 (MQ=255)
                                               atatAGATATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATc                                                                                                    <  2:3237797/150‑1 (MQ=255)
                                               atatAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATATCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATc                                                                                                    >  2:1740672/1‑150 (MQ=255)
                                                tataGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCa                                                                                                   <  2:2380560/150‑1 (MQ=255)
                                                tataGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCa                                                                                                   <  2:2380522/150‑1 (MQ=255)
                                                tataGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCa                                                                                                   <  2:2207287/150‑1 (MQ=255)
                                                  taGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGTAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATg                                                                                                 <  2:297074/150‑1 (MQ=255)
                                                  taGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATg                                                                                                 <  2:1535722/150‑1 (MQ=255)
                                                    gagaTTTAAGCCAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGgc                                                                                               <  2:2540262/150‑1 (MQ=255)
                                                       aTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTGTTTTTTATTTATAAAAAAATTAAAAAAAAAAAAAAAAAAATAGATGTAAAACACGAATATTAAAATTAGCGCCCCACAGGgcgcg                                                                                            >  1:1564996/1‑150 (MQ=255)
                                                          tAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTTATTTATAAAAAAAATAAAAAAAAAAAAAACAAAACAGATGTAAATCACAAAATTTAAAATTAGCGCCCCACAAGgcgcgccc                                                                                         >  1:1827906/1‑148 (MQ=255)
                                                          tAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGcac                                                                                         >  2:2747012/1‑150 (MQ=255)
                                                                    tttatttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTataat                                                                               <  2:3380457/150‑1 (MQ=255)
                                                                     ttatttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATCGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTataata                                                                              <  2:243306/150‑1 (MQ=255)
                                                                         ttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCaa                                                                          <  2:1829776/150‑1 (MQ=255)
                                                                         ttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCaa                                                                          <  2:1829696/150‑1 (MQ=255)
                                                                           ggattttATTTTTGTTTCGACATTTGATTATACATAACTGTCTTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGGGATGTAACGCACTAATTTTAAATTGCGCGCAAATCTTGGCGCGCACAAGCTATAATACCAAcc                                                                        <  1:3397609/144‑1 (MQ=255)
                                                                            gAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAAAAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAAcca                                                                       >  2:1028203/1‑149 (MQ=255)
                                                                               tGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAAATAAAAGAAAACAAGACAAAAGCGATGTGAATCAAAAGGTTTTAATTGGGCGCCAAAAAAGGCGCGCCCAAGCGATAATACCAACCaaaa                                                                    >  2:1433176/1‑149 (MQ=255)
                                                                                   tttttCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTTAAAAAAAATTCAAAGAAATAAAGACAAAAGCGATGTAAACCACGAAGTTTAAAATTCGCGCCAATCATTGCGCGCAAAACCTATAAAACCCACCAAAtttat                                                                >  2:800883/1‑148 (MQ=255)
                                                                                   tttttCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAAAAAAGACAATATCGATGTAAAGCACTAATTTTAAATTGAGCGCCAATAAAGGCGCGCACAAACTATAATACCAAACTAATTtct                                                                >  2:1637648/1‑150 (MQ=255)
                                                                                            cgACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCtt                                                       >  2:2302775/1‑150 (MQ=255)
                                                                                            cgACCTTTGCTTATACATAACAGTCGTTTTTTTAAATTATAAAATAATTCAAAGAAATCAAGACAATATCGATGTAAAGCACTAAGTTTAAATTTCGCGCCAATCATTGCGCGCACAAGCAATAATACCAACCAAATTTCTCCTCCTCtt                                                       >  2:2507150/1‑150 (MQ=255)
                                                                                             gACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAATTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTa                                                      <  1:867997/150‑1 (MQ=255)
                                                                                              aCCTTTGCTTATAAATAACAGTCGTTTTTTTAATTTTTAAAATAATTCAAAGACATCAAGACATTAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAATCTATTATACCAACCTAATTTGTCATCCTCTTag                                                     <  2:709434/150‑1 (MQ=255)
                                                                                                 tttGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGt                                                  <  2:2082926/150‑1 (MQ=255)
                                                                                                   tGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGa                                                <  2:2442392/150‑1 (MQ=255)
                                                                                                    gCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGAc                                               <  2:1970580/150‑1 (MQ=255)
                                                                                                     cTTATAAATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAATAAATCAAGACAATAGCGATTTAAAGAACTAATTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACt                                              <  1:3977760/150‑1 (MQ=255)
                                                                                                     attATACATAACAGTCTTTTTTTTAATTTATAAAATAATTCAAAGAAATCAATACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACt                                              <  1:3951546/149‑1 (MQ=255)
                                                                                                      ttATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCGATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACta                                             >  2:594357/1‑150 (MQ=255)
                                                                                                         taCATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTatat                                          <  2:1707974/150‑1 (MQ=255)
                                                                                                            aTAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAAACAAGACAAAAGCGATGTAAAGAACTAAGTTTAAATTTAGCGCCAATCATGGCGCGCACAAGCTATAAAACAAACCAAATTTCTCCACCTCTTAGAGTGACTATAactc                                       >  2:3336491/1‑150 (MQ=255)
                                                                                                             tAACAGTCGTTTTTTTAATTTATAAAATAATTGAAATTAATAAATACAAAATAGATTTTAATGAGTAAGTTTAAATTTGGCGCCAATGGTGGGGCGCACAAGCTATAATAAAAACCTAATTTTTACTCCTCTTAGTGTGAGTatatataa                                      >  2:2817923/1‑146 (MQ=255)
                                                                                                             tAACAGTCGTTTTTTTAATTTATAAAAAAAATCAAAGAAAACAAGACAAAAGCGATGTAAAGCACTAATTTTAAATTTCGCGCCAAACATTGCGCGCCCAACCTATAATAACAAACTAAATTATCCTCCTCTTATAGTTACTATAtatca                                      >  2:2960100/1‑149 (MQ=255)
                                                                                                              aaCAGTCGTTTTTTTAATTTATAAAATAATTAAAATAAAACAAGAAAAAATCGATTTAAATGACAAAGTTTAAATTTGGCGCCCATCATGGCGCGCACAAGCTATAAAAACAAACAAATTTCTACACCACTTAGAGTGACTatatagccg                                     >  2:3893255/1‑145 (MQ=255)
                                                                                                              aaCAGTCGTTTTTTTAATTTAAAAAATAATTAAAAGAAAACAATACAAAAGCGAAGTAAACCACAAAGTTTAAAATGCGCGCCAAACATGGCGCGCACAATCTATAATACCAACATAAATTATACACCGCTTAGTGTGACAAAATCTACt                                     >  2:570560/1‑150 (MQ=255)
                                                                                                                  gTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATa                                 <  1:1194825/150‑1 (MQ=255)
                                                                                                                  ctCGTTTTTATAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGATTAAATTGCGCGCCAAACATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATa                                 <  2:1034320/149‑1 (MQ=255)
                                                                                                                     gTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGaa                              >  1:3018724/1‑150 (MQ=255)
                                                                                                                      tttttttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAAt                             >  2:4106960/1‑150 (MQ=255)
                                                                                                                      tttttttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAAt                             >  1:2669434/1‑150 (MQ=255)
                                                                                                                      tttttttAATTTATAAAATAATCCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAAt                             >  2:4107046/1‑150 (MQ=255)
                                                                                                                          tttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCg                         <  2:804748/150‑1 (MQ=255)
                                                                                                                                 tataAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGa                  <  2:3571623/150‑1 (MQ=255)
                                                                                                                                 tataAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGa                  <  2:290092/150‑1 (MQ=255)
                                                                                                                                    aaaaTAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTa               >  1:1415720/1‑150 (MQ=255)
                                                                                                                                     aaaTAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTAt              >  2:2600602/1‑150 (MQ=255)
                                                                                                                                     aaaTAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTAt              <  1:918690/150‑1 (MQ=255)
                                                                                                                                      aataatTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATc             >  1:1917883/1‑150 (MQ=255)
                                                                                                                                      aataatTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATc             >  2:1255292/1‑150 (MQ=255)
                                                                                                                                         aatTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCaaa          <  1:1850445/150‑1 (MQ=255)
                                                                                                                                            tCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGc       >  2:37284/1‑150 (MQ=255)
                                                                                                                                            tCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGc       >  2:372279/1‑150 (MQ=255)
                                                                                                                                            tCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGc       <  1:2286511/150‑1 (MQ=255)
                                                                                                                                             cAACGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGcc      <  2:3168818/150‑1 (MQ=255)
                                                                                                                                             cAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGcc      <  1:3086288/150‑1 (MQ=255)
                                                                                                                                              aaaGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGTCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGccc     <  1:2204713/150‑1 (MQ=255)
                                                                                                                                              aaaGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGccc     >  2:3024123/1‑150 (MQ=255)
                                                                                                                                               aaGTAATCTAGACAATAGCGATTTAAAGCACTATGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCTAATGCCCt    <  1:2435542/150‑1 (MQ=255)
                                                                                                                                               aaGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCTAATGCCCt    <  1:2435477/150‑1 (MQ=255)
                                                                                                                                               aaGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCt    <  1:3720612/150‑1 (MQ=255)
                                                                                                                                               aaGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCt    >  2:542836/1‑150 (MQ=255)
                                                                                                                                                 gAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGa  <  2:3819032/150‑1 (MQ=255)
                                                                                                                                                 gAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGa  >  2:1887466/1‑150 (MQ=255)
                                                                                                                                                 |                                                                                                                                                     
TCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGA  >  NC_000913/2470286‑2470580

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: