Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,046,050 T→G 9.0% intergenic (‑149/+118) ygfF ← / ← gcvP putative NAD(P)‑dependent oxidoreductase/glycine decarboxylase, PLP‑dependent, subunit P of glycine cleavage complex

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,046,0500TG 9.0% 493.6 / 5.8 206intergenic (‑149/+118)ygfF/gcvPputative NAD(P)‑dependent oxidoreductase/glycine decarboxylase, PLP‑dependent, subunit P of glycine cleavage complex
Reads supporting (aligned to +/- strand):  ref base T (64/117);  new base G (10/14);  total (75/131)
Fisher's exact test for biased strand distribution p-value = 6.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 213 total aligned reads displayed.

TTTGTTTTTCCGAGAAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAG  >  NC_000913/3045901‑3046198
                                                                                                                                                     |                                                                                                                                                    
tttGTTTTTCCGAGAAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGt                                                                                                                                                      >  2:3568512/1‑150 (MQ=255)
 ttGTTTTTCCGAGAAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGtt                                                                                                                                                     >  1:2131415/1‑150 (MQ=255)
  ttttttcCCGATAAAAATTATTTCGTTGACTTATCTTTGAGATTGATAACAAACTGGCTCATTTTTCATTGTGGGCACGCTTTTCCCCCTCGCCCTTTCTGGGTGTGGGCGGGGGTGAGGGTAAATATACGCGCCAGTGCCGGCCTGTTc                                                                                                                                                    <  1:450533/148‑1 (MQ=255)
    tttttCCGAGAAAAATTAAGTCGTTGACGTATCTTTGAGATTGCTACCACACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTccc                                                                                                                                                  <  2:2235701/150‑1 (MQ=255)
        tCCGAGAAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCa                                                                                                                                              <  2:824669/150‑1 (MQ=255)
        tCCGAGAAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCa                                                                                                                                              <  2:3539938/150‑1 (MQ=255)
          cGAGAAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCAcc                                                                                                                                            >  2:3854748/1‑150 (MQ=255)
            agaAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTACCCACAccca                                                                                                                                          >  2:204356/1‑149 (MQ=255)
                aaaTTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGGTCCCCGCACCCAAAcc                                                                                                                                      >  2:52427/1‑150 (MQ=255)
                 aattaattCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCCGTGCCGGCCTGTTCCCCTCACCCTAAccc                                                                                                                                     >  2:3608443/1‑150 (MQ=255)
                      attCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTcc                                                                                                                                <  2:803271/150‑1 (MQ=255)
                      attCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTcc                                                                                                                                <  2:2423337/150‑1 (MQ=255)
                           ttGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCACACCCTAACCCACTCCCCcaa                                                                                                                           >  2:298713/1‑150 (MQ=255)
                            tGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAg                                                                                                                          <  2:1478565/150‑1 (MQ=255)
                                gTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGc                                                                                                                      <  1:3169072/150‑1 (MQ=255)
                                    cTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAgg                                                                                                                  <  1:170769/150‑1 (MQ=255)
                                    cTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCGCCCCTTTGGGGAGAgg                                                                                                                  <  2:2008342/150‑1 (MQ=37)
                                    cTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCGCCCCTTTGGGGAGAgg                                                                                                                  <  1:3046425/150‑1 (MQ=37)
                                     tttGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCCCCCAAACCCACACCCCCAAGGGGCGAggg                                                                                                                 >  1:116748/1‑150 (MQ=255)
                                     tttGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAggg                                                                                                                 >  2:4102077/1‑150 (MQ=255)
                                         agaTTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAACTATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACt                                                                                                             <  2:1201966/150‑1 (MQ=255)
                                         agaTTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGGTACCCGCACCCAAACCCACACCCCCACAGGGCGAggggccg                                                                                                             >  1:1813461/1‑147 (MQ=255)
                                          gaTTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGGTCCCCACACCCCCACCCACACCCCCAAGGGGCGAGGGGAcgg                                                                                                            >  1:302474/1‑148 (MQ=255)
                                           aTTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTGTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCGCCCCTTTGGGGAGAgggttaggg                                                                                                           <  2:3786827/150‑7 (MQ=37)
                                            ttGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCACACCCTAACCCACTCCCCCAAGGGGCGAGGGGACTGTc                                                                                                          >  2:2152136/1‑150 (MQ=255)
                                              gCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCACACCCTAACCCGCTCCCCAAAGGGGCGAGGGGGCTGTCTg                                                                                                        >  1:93748/1‑150 (MQ=255)
                                               cTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGa                                                                                                       <  2:1640995/150‑1 (MQ=255)
                                                tAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAg                                                                                                      >  2:4047478/1‑150 (MQ=255)
                                                tAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCGGGTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAg                                                                                                      >  2:2307667/1‑150 (MQ=255)
                                                 aaCAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGc                                                                                                     >  2:1286616/1‑150 (MQ=255)
                                                 aaCAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCGAACCCACTCCCCAAAGGGGCGAGGGGACTGTCTGAGc                                                                                                     >  1:3364286/1‑150 (MQ=255)
                                                  aCAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCACACCCTAACCCACTCCCCAAAGGGGCGCGGGGGCAGGCTGAGCa                                                                                                    >  2:396618/1‑150 (MQ=255)
                                                   cAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGGTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCAc                                                                                                   <  1:2109798/150‑1 (MQ=255)
                                                       cTGGCTCACTCGACCATGTTGGCACGCTTTTCCCCCTCGCCCGTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCCCCCTAACCCTCTCCCCAAAGGGGCGTGGGGACTGTCTGAGCACtttt                                                                                               <  2:2404025/150‑1 (MQ=25)
                                                       cTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCACAGGGGCAAGGGGACTGTCTGAGCACtttt                                                                                               <  2:2424281/150‑1 (MQ=255)
                                                       cTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACtttt                                                                                               <  2:1836594/150‑1 (MQ=255)
                                                          gCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTAAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTACCCTCACCCTAACCCAATCCCCCAAGGGGGGAGGGGGCAGGCTGAGCCCGTGTGTa                                                                                            >  2:1150420/1‑150 (MQ=255)
                                                           cTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTAc                                                                                           >  2:3373044/1‑150 (MQ=255)
                                                              aaTCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACttt                                                                                        <  2:3347936/150‑1 (MQ=255)
                                                              aaTCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACttt                                                                                        <  2:1405004/150‑1 (MQ=255)
                                                              aaTCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACttt                                                                                        <  1:1361927/150‑1 (MQ=255)
                                                              aaTCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACttt                                                                                        <  1:1030425/150‑1 (MQ=255)
                                                               aTCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTg                                                                                       <  1:2815553/150‑1 (MQ=255)
                                                               aTCTTCAATGTGGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTg                                                                                       <  1:2100615/150‑1 (MQ=255)
                                                                tCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGt                                                                                      <  1:1473962/150‑1 (MQ=255)
                                                                 cTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTc                                                                                     <  2:1249740/150‑1 (MQ=255)
                                                                    cAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCGCACCCTAACCCGCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCCCGGTTGTAATTTGTCAta                                                                                  >  1:2993165/1‑149 (MQ=25)
                                                                     aaTGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCt                                                                                 <  1:3685102/150‑1 (MQ=255)
                                                                     aaTGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCt                                                                                 <  1:4099539/150‑1 (MQ=255)
                                                                       tGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGa                                                                               <  1:3568512/150‑1 (MQ=255)
                                                                       tGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGa                                                                               <  2:257458/150‑1 (MQ=255)
                                                                         ttGGCACTCTTTTCCCCCTCGACCTTTCTGGTAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCTCCCTTACCCTCTACCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACt                                                                             <  1:44956/150‑1 (MQ=255)
                                                                              aCGCTTGTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTaaaaa                                                                        <  2:3061589/150‑1 (MQ=255)
                                                                               cGCTTGTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAg                                                                       <  1:698733/150‑1 (MQ=255)
                                                                                gCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTaaaaatg                                                                      >  2:2732544/1‑148 (MQ=255)
                                                                                gCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAgg                                                                      >  2:661613/1‑150 (MQ=255)
                                                                                     tCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCAGACTAAAAAGGCGCCg                                                                 >  2:317100/1‑150 (MQ=255)
                                                                                       ccccTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTACCCACACCCAAAACCACTACCCAAATGGGCGCGGGGGCGGGCTGTGCACATTTGTACGTTTTCAACTGAAAAAAAAAGCGCCCaa                                                               >  1:2120330/1‑150 (MQ=255)
                                                                                        cccTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAACTATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAg                                                              <  2:2227985/150‑1 (MQ=255)
                                                                                        cccTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTGCCCGCACCCAAACCCACAACCCCAACGGGCGAGGGGCCTGTCTGAGCACTGTTGTAAGTTGTAATCAGACAAAAAATTCGCCCAAg                                                              >  1:2015741/1‑150 (MQ=255)
                                                                                          cTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAgcg                                                            <  1:627415/150‑1 (MQ=255)
                                                                                          cTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAgcg                                                            <  2:2484688/150‑1 (MQ=255)
                                                                                           tCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAgcgc                                                           <  1:3284703/150‑1 (MQ=255)
                                                                                           tCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAgcgc                                                           <  2:449116/150‑1 (MQ=255)
                                                                                           tCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCGCCCCCGAAACCACACCCCCAAGGGGCGAGGGGACTGTCTGAGCACGTTTGTACTTTTTAATATTACTAAAAATGCGCCGAAgcgc                                                           >  1:3956833/1‑150 (MQ=255)
                                                                                            cGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGcc                                                          <  1:3883729/150‑1 (MQ=255)
                                                                                             gCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCt                                                         <  2:44678/150‑1 (MQ=255)
                                                                                              tacTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCtt                                                        <  2:3241600/148‑1 (MQ=255)
                                                                                                cTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTa                                                      <  1:3245793/150‑1 (MQ=255)
                                                                                                cTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTa                                                      <  2:3885049/150‑1 (MQ=255)
                                                                                                 tttCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTGCCCTCACCCTAAACCACACCCCAAAGGGGGGTGGGGTCTGTGTGAGCACTTTTTTTCTTTTTGATGTTACTAAAAATGGGCCGAAGCGGCTTTat                                                     >  1:1305946/1‑149 (MQ=255)
                                                                                                 tttCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAg                                                     <  1:3468450/150‑1 (MQ=255)
                                                                                                  ttCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGa                                                    <  2:392439/150‑1 (MQ=255)
                                                                                                  ttCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGa                                                    <  2:1103834/150‑1 (MQ=255)
                                                                                                     aGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGaaaa                                                 <  1:3208711/150‑1 (MQ=255)
                                                                                                       ggAGAGGGCCGGGGTGAGGGTAAATCTTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATa                                               <  1:52427/150‑1 (MQ=255)
                                                                                                       ggAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGGTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAACATa                                               <  1:245893/150‑1 (MQ=255)
                                                                                                       ggAGAGGGCCGGGGTGAGGGGACCTAGTCGCGCCAGTGCCGGCCGGGTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCCTCTGACTCAAAAGGCGCCGAAGCGCCTTTAGAAAATa                                               <  2:1012938/150‑1 (MQ=37)
                                                                                                          gagGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGATCCCCTAACCCAAACCCACTCCCCAAAGGGGCGAGGGGACTGTCTGAGCCCGTTTTTTCGTTGTCAACAGACTAAAAACGCGCCCAAGCGCCGGTAGAAAAAAggc                                            >  2:1095158/1‑148 (MQ=21)
                                                                                                              gCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAAt                                        <  2:4106773/150‑1 (MQ=255)
                                                                                                              gCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAAt                                        <  1:3454561/150‑1 (MQ=255)
                                                                                                                cGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCa                                      <  2:3956833/150‑1 (MQ=255)
                                                                                                                 ggggTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAg                                     <  2:1570563/150‑1 (MQ=255)
                                                                                                                  gggTGAGGGTAAATCTTCGCGCCAGTGCCGGCCTGGGCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGt                                    <  1:4075677/150‑1 (MQ=255)
                                                                                                                  gggTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGt                                    <  2:1813406/150‑1 (MQ=255)
                                                                                                                          gTAAATCTTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCTAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACt                            <  1:3154999/150‑1 (MQ=255)
                                                                                                                          gTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACt                            <  2:760199/150‑1 (MQ=255)
                                                                                                                          gTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACt                            <  2:2874484/150‑1 (MQ=255)
                                                                                                                          gTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCACACCCAAAACCACACCCCCAAGGGGCGAGGGGACTGTCTGAGCCCCGTTGTACGTTGGAAAATGACTAAAAAGGCGCCCAAGCGCCGTGAGAAAAAAGTCGAATAAGTGAAATACt                            >  2:3279863/1‑150 (MQ=255)
                                                                                                                           tAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACACTAACCCACTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAATGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTg                           >  2:599637/1‑150 (MQ=255)
                                                                                                                           tAAATATTCGCGCCAGTGCCGGCCTGTTCCCCAAAACCAAAACCACAACCCAAAGGGGCGAGGGGACTGTCTTATCACTTTTTTAATTTTTCATCTTAATAAAAAAGCGCCGAAGCGCCTTTAGAAAATAGTCGAATAACTGAATTACtt                           >  2:29959/1‑149 (MQ=255)
                                                                                                                            aaaTATTCGCGCCAGTGCCGGCCTGGTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTgg                          <  1:3598413/150‑1 (MQ=255)
                                                                                                                              atatTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTa                        <  2:1032681/150‑1 (MQ=255)
                                                                                                                                atTCGCGCCAGTGCCGGCCTGGTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTAtt                      <  1:204356/150‑1 (MQ=255)
                                                                                                                                 ttCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTTTACTTTGTCATCTGAATAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTAATTGTATTc                     >  2:2297546/1‑150 (MQ=255)
                                                                                                                                   cgcgCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGc                   <  1:1615875/150‑1 (MQ=255)
                                                                                                                                        cAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATc              <  1:2281368/150‑1 (MQ=255)
                                                                                                                                                   tGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGa   >  2:627275/1‑150 (MQ=255)
                                                                                                                                                    gTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAg  >  2:145163/1‑150 (MQ=255)
                                                                                                                                                    gTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAg  <  1:2718569/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TTTGTTTTTCCGAGAAAAATTAATTCGTTGACGTATCTTTGAGATTGCTAACAAACTGGCTCAATCTTCAATGTTGGCACGCTTTTCCCCCTCGCCCTTTCAGGGAGAGGGCCGGGGTGAGGGTAAATATTCGCGCCAGTGCCGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACTGTCTGAGCACTTTTGTACTTTGTCATCTGACTAAAAAGGCGCCGAAGCGCCTTTAGAAAATAGTCGAATCAGTGAATTACTGGTATTCGCTAATCGGTACGCAGGAG  >  NC_000913/3045901‑3046198

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: