Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,390,012 Δ1 bp 5.2% intergenic (+48/+36) yfbP → / ← nuoN TPR‑like repeats‑containing protein/NADH:ubiquinone oxidoreductase, membrane subunit N

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,390,0120G. 5.2% 238.1 / 7.2 77intergenic (+48/+36)yfbP/nuoNTPR‑like repeats‑containing protein/NADH:ubiquinone oxidoreductase, membrane subunit N
Reads supporting (aligned to +/- strand):  ref base G (36/37);  new base . (2/2);  total (38/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.67e-01

GCATTTCAGGAACGCTATGATTTTAATATCGATGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGATACCGCCCGCGCTG  >  NC_000913/2389863‑2390156
                                                                                                                                                     |                                                                                                                                                  
gCATTTCAGGAACGCTATGATTTTAATATCGATGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAg                                                                                                                                                    >  2:661004/1‑150 (MQ=255)
gCATTTCAGGAACGCTATGATTTTAATATCGATGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAg                                                                                                                                                    >  2:613229/1‑150 (MQ=255)
        ggAACGCTATGATTTTAATATCGATGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTcgg                                                                                                                                           <  1:896863/150‑1 (MQ=255)
        ggAACGCTATGATTTTAATATCGATGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTACACTTAACGCTCTGCGTATATCTAATATACCTTCTTTTTCAGAAAAACCGCCGAGCAAGTcgg                                                                                                                                           >  2:195648/1‑150 (MQ=255)
         gAACGCTATGATTTTAATATCGATGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTcggc                                                                                                                                          <  1:375057/150‑1 (MQ=255)
            cGCTATGATTTTAAAATCGATGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGACATGCTcggcggt                                                                                                                                       <  2:1177469/150‑2 (MQ=255)
            agCTATTATTTTAATCTCGATGAATGGCGGGCAAAATTCAGGCAGGTATTTTTTGAGTAGGTTGGCGATAGTCCTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGTGCATGTCggcggc                                                                                                                                      <  2:801153/149‑1 (MQ=255)
              cTATGATTTTAATATCGATGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCtt                                                                                                                                    <  1:1314909/150‑1 (MQ=255)
              cTATGATTTTAATATCGATGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCtt                                                                                                                                    <  2:1198148/150‑1 (MQ=255)
                aTGATTTTAATATAGATGAATGGCGTGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGGCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTc                                                                                                                                  <  1:109458/150‑1 (MQ=255)
                 tGATTTTAATATCGATGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCGTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCt                                                                                                                                 >  1:806522/1‑150 (MQ=255)
                 tGATTTTAATATCGATGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCt                                                                                                                                 >  2:484953/1‑150 (MQ=255)
                   aTTTTAATATCGATGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAGCCGCCGACATGCTCGGCGGtttttctga                                                                                                                              <  1:860736/150‑6 (MQ=38)
                             cGATGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAACACCGCCGCGCATGTCGGCGGCTTTCTGACTTACAAAGt                                                                                                                     >  1:501202/1‑150 (MQ=255)
                                tGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAAc                                                                                                                  <  2:686724/150‑1 (MQ=255)
                                 gAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACa                                                                                                                 <  2:955795/150‑1 (MQ=255)
                                      gctggCATAATTGACGCTGTTATGTTTAGAGTATTTTTGCGATAGTCGTACACAGTTCACTTAATGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGAATGTGGGCGGCTTTCTGACTTACAAAGTAACAGATTa                                                                                                            <  2:574352/147‑1 (MQ=255)
                                       cGGGCAAAATTCAGGCAGTTATGTGTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCAAGTCGGCGGCTTTCTGACTTACAAAGTAACAGATGAc                                                                                                           >  2:207044/1‑150 (MQ=255)
                                         ggCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAACCCGCCGAGCATGGCGGAGGCTTTCTGACTTACAAAGTACCAGATTACAt                                                                                                         >  2:490078/1‑150 (MQ=255)
                                         ggCAAAATTCAGGCAGTTAGGTTTAGAGTATTTTGGCGATAGTCGTGCACAGGTCACTTAACGCTCTGCGTCTATCTAGTAGCCCTTCTTTTTCAGAAAAACCGCGGGGCTTGTCGGGGGCTTTCTGGCTGAGAAAGGAACGGGTTAcct                                                                                                         >  2:851578/1‑148 (MQ=255)
                                          gCAATATTAAGGCAGTGATGTTTAGAGTATTTTGGAGATAGTCGTACACATTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATc                                                                                                        <  2:1170106/150‑1 (MQ=255)
                                          gCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATc                                                                                                        <  2:1051371/150‑1 (MQ=255)
                                                 tCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCa                                                                                                 <  2:856226/150‑1 (MQ=255)
                                                 tCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCa                                                                                                 <  2:194909/150‑1 (MQ=255)
                                                     gCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTTACAGATTACATCAGCGGCATTGc                                                                                             <  1:195887/150‑1 (MQ=255)
                                                     gCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCAGAGCATATCGGCGGCTTTCTGACATAAAAAGTAACAGATTACATCAGCGGCAATGc                                                                                             >  2:1189227/1‑150 (MQ=255)
                                                     gCAGCTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGc                                                                                             <  1:451158/150‑1 (MQ=255)
                                                       aGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCa                                                                                           >  1:772052/1‑150 (MQ=255)
                                                        gTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCaa                                                                                          >  2:775656/1‑150 (MQ=255)
                                                           aTGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGGCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACg                                                                                       <  1:1160301/150‑1 (MQ=255)
                                                            tGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGc                                                                                      >  1:1161203/1‑150 (MQ=255)
                                                                tAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACaa                                                                                  >  2:1239757/1‑150 (MQ=255)
                                                                  gagTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATg                                                                                <  1:661004/150‑1 (MQ=255)
                                                                   agTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGc                                                                               <  1:261585/150‑1 (MQ=255)
                                                                      aTTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTaa                                                                            >  2:554907/1‑150 (MQ=255)
                                                                      aTTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATATACTATCCATTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGAACAATGCTaa                                                                            >  1:1245596/1‑150 (MQ=255)
                                                                       ttttGGCGATAGTCGTACACAGTTCACTTAAGGCGCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGGCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAAt                                                                           <  2:483583/150‑1 (MQ=255)
                                                                        tttGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATc                                                                          >  2:286577/1‑150 (MQ=255)
                                                                        tttGGCGATAGTCGTACACAGTTCACATAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCTCCGAGCATGTCGGCGGCATTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATc                                                                          <  1:286375/150‑1 (MQ=255)
                                                                         ttGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCa                                                                         >  1:1026407/1‑150 (MQ=255)
                                                                          tGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCAGGGCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCCGCGGCATTGCCAAACGCACAATGCTAATCAg                                                                        >  2:798596/1‑150 (MQ=255)
                                                                            gCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTAAATCAGCGGCATTGCCAAACGCACGATGCTAATCAGCg                                                                      >  1:194166/1‑150 (MQ=255)
                                                                              gATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGt                                                                    <  2:1267351/150‑1 (MQ=255)
                                                                                 aGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTtgt                                                                 >  1:1189901/1‑150 (MQ=255)
                                                                                   tCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTgg                                                               <  2:1037936/150‑1 (MQ=255)
                                                                                          caGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATacc                                                        >  1:524793/1‑149 (MQ=255)
                                                                                          caGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCAAGTCGGCGGCATTCTGACTTACAAAGTAACAGATTACATCAGCGTCATTGCCAAACGCACAATACTAATCAGCGGTTGTGGCCAAaca                                                        >  2:62198/1‑150 (MQ=255)
                                                                                           aGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATacac                                                       <  1:361766/150‑1 (MQ=255)
                                                                                           aGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATacac                                                       >  1:414372/1‑150 (MQ=255)
                                                                                              tCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCa                                                    <  2:1012920/150‑1 (MQ=255)
                                                                                               cACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAAAAGATTACATCAGCGGCATTGCCAAACGCACAATTCTAATCAGCGGTTGTGGCCATACACCCAg                                                   >  1:753961/1‑150 (MQ=255)
                                                                                                agttAACTCTCTGGGTGTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAAAAGATTACATCAGCGGCATTGCCAAACGCACAATTCTAATCAGCGGTTGTGGCCATACACCCAgg                                                  <  2:543046/148‑2 (MQ=255)
                                                                                                   tAACGCTCTGCGTCTATCTACTATCCCTTCTATTTCAGAAAAACCGCCGAGCATGGCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCAcc                                               <  2:124421/150‑1 (MQ=255)
                                                                                                      cGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCTGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGt                                            <  1:712664/150‑1 (MQ=255)
                                                                                                                ctatctaCTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACATAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATCCACCCAGCACCAGTACCCACAGTg                                  >  2:550200/1‑150 (MQ=255)
                                                                                                                ctatctaCTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTg                                  <  2:438653/150‑1 (MQ=255)
                                                                                                                 tatctaCTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACAACAGCGGCATTGCCAAACGCACAATGCTAATCAGCGTTTGTGGCAATACACCCAGCACCAGTACCAACAGTGc                                 >  1:619253/1‑150 (MQ=255)
                                                                                                                      actaTCCCTTCTTTTTCAGAACAACCGCCGAGCATGGCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAgaga                            <  2:459139/150‑1 (MQ=255)
                                                                                                                      actaTCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCTGCTTTCTGACTTACAAAGTAACAGATTACATCAGAGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGAGGCCATACACCCAGAACCAGTACAAACAGAGCAgaga                            >  1:198386/1‑150 (MQ=255)
                                                                                                                         aTCCCTTCTTTTTCAGAAAAACCGCCGCGCATGGCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCa                         <  1:697583/150‑1 (MQ=255)
                                                                                                                         aTCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCa                         >  2:456688/1‑150 (MQ=255)
                                                                                                                          tCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAg                        >  1:26524/1‑150 (MQ=255)
                                                                                                                           cccTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGc                       >  1:579153/1‑150 (MQ=255)
                                                                                                                            ccTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGTTTACATCAGCGGCATTGCCAATCGCACAATGCTAATCAGCGGTTGTGGCCATACAGCCAGCACCAGTACCAACAGTGCAGAGATCAGca                      >  1:63911/1‑150 (MQ=255)
                                                                                                                            ccTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCTTTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGca                      <  2:392164/150‑1 (MQ=255)
                                                                                                                            ccTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGca                      <  2:1210263/150‑1 (MQ=255)
                                                                                                                              ttcttTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGcaca                    <  1:574806/150‑1 (MQ=255)
                                                                                                                                    agtcagaaagCCGCCGACATGCTCGGCGGTTTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGAt               >  1:677833/7‑150 (MQ=38)
                                                                                                                                    agtcagaaagCCGCCGACATGCTCGGCGGTTTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGAt               >  1:783312/7‑150 (MQ=38)
                                                                                                                                     tCAGAAAAACGGCCGAGGATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCAGAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGATAc             <  1:80365/150‑1 (MQ=255)
                                                                                                                                     tCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGTCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGATAc             <  1:83390/150‑1 (MQ=255)
                                                                                                                                      cAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGATAcc            >  1:1099758/1‑150 (MQ=255)
                                                                                                                                       aGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGATAccg           <  2:1161203/150‑1 (MQ=255)
                                                                                                                                       aGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGATAccg           >  1:328563/1‑150 (MQ=255)
                                                                                                                                        gAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACAGCAGCGGCATTGCCACACGCACATTGCTAATCCGCGGTTTTGGCCATACACCCCGCACCAGTACCACCAGTGCAGAGATCAGAACAACGATTccgc          >  1:865171/1‑150 (MQ=255)
                                                                                                                                          aaaaCCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGATAccgccc        >  1:863141/1‑150 (MQ=255)
                                                                                                                                           aaaCCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGATAccgcccg       >  1:896718/1‑150 (MQ=255)
                                                                                                                                                gccgAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACCACGATACCGCCCGCGCTg  >  2:747932/1‑150 (MQ=255)
                                                                                                                                                gccgAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGATACCGCCCGCGCTg  <  1:1132746/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
GCATTTCAGGAACGCTATGATTTTAATATCGATGAATGGCGGGCAAAATTCAGGCAGTTATGTTTAGAGTATTTTGGCGATAGTCGTACACAGTTCACTTAACGCTCTGCGTCTATCTACTATCCCTTCTTTTTCAGAAAAACCGCCGAGCATGTCGGCGGCTTTCTGACTTACAAAGTAACAGATTACATCAGCGGCATTGCCAAACGCACAATGCTAATCAGCGGTTGTGGCCATACACCCAGCACCAGTACCAACAGTGCAGAGATCAGCACAACGATACCGCCCGCGCTG  >  NC_000913/2389863‑2390156

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: